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The Ⅶ International Symposium on the Nutrition of Herbivores(ISNH-7)

作者： Y.Q.Zhang X.Z.Deng Z.M.Zhou L.P.Ren Q.X.Meng~1 State Key Laboratory of animal Nutrition beef cattle research center and college of animal science and technology China Agricultural University Beoing 100094 P.R.China

＜正＞A feeding trial using 12 Holstein bull calves（21±3 d）was conducted to investigate the effect of different processed maize grain and full fat soyabean included in the starter diets on their rumen fermentation and *** treatment starter diets contained different processed maize grain and soyabean:steam-flaked maize and soyabean（SFMS）,extruded maize and soyabean （EMS）,and ground maize and raw soyabean（GMS）.During 10-week trial,total rumen VFA concentration tended to be influenced by different processing methods at wk 6 and 9（P＜0.10）. The molar proportion of butyrate for calves receiving SFMS starter diets was higher than that of EMS and GMS starter diets（P＜0.05）at wk 5 and *** papillae development characteristics expressed by papilla length,papilla width and rumen wall thickness were remarkably different among treatment starter diets（P＜0.05）.

关键词： calves processing maize grain full fat soyabean rumen development

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Effect of supplemental β-carotene in the periparturient diet on plasma vitamin A and β-carotene concentrations and lymphocyte proliferation in Holstein cows

Effect of supplemental β-carotene in the periparturient die...

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The Ⅶ International Symposium on the Nutrition of Herbivores(ISNH-7)

作者： W.J.He~1 Q.D.Zhang~2 L.P.Ren~1 Q.X.Meng~(1 3) ~1State Kev Laboratory of animal Nutrition beef cattle research center and college of animal science and technology China Agricultural University Beijing 100094 P.R.China ~2Chinese Academy of Agricultural Engineering Beijing 100026 P.R.China

＜正＞Thirty six lactating Holstein cows were selected based on identical parity,milk yield,body weight and body condition score to study the effect of supplemental β-carotene in the periparturient diet on plasma vitamin A（VA）and β-carotene concentrations and lymphocyte *** cows were randomly allotted into three groups and individually fed with one of three treatment diets:*** diet（basic diet+150,000 IU VA/cow/day）,*** diet+300 mg β-carotene/ cow/day,and *** diet+600 mg β-carotene/cow/*** results showed that supplemental β-carotene increased the concentration of VA and β-carotene in plasma,and increased the lymphocyte proliferation with adequate VA in the diet during periparturient period（P＜0.01）.

关键词： β-carotene plasma lymphocyte proliferation cows

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Artificial Induction of Twinning by an Active Immunization of beef Cows Against Inhibin Partially Purified from Porcine Seminal Plasma

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Agricultural sciences in China 2002年第4期1卷 466-471页

Two hundred and seventy multiparous Chinese Yellow cattle (beef) were selected at 1 to 3 months postpartum and divided into three groups (90 cows for each). animals were given both a primary and booster immunizations with a total dose of 3 mg (Group Th) or 1.5 mg (Group Tl) of seminal preparation containing inhibin activity, emulsified with Freund's complete adjuvant and incomplete adjuvant (for booster) , at 3 or 4-week intervals. Other cows were treated with the same volume of seminal preparation without inhibin activity as procedures mentioned above to serve as a control (Group C). Artificial inseminations were given twice at 8 - 12 h intervals when the cow was in heat. Jugular venous blood samples were collected from each cow and used to assay the presence of antibody against seminal preparation by double-diffusion in agar precipitation test and to detect the titer of inhibin antibody by an ELISA method. Data from 247 cows showed that 83.9% (73/87) of cows were in estrus and ovulated 89 ova altogether, of which 19 cows ovulated twin ova and 15 cows produced twins in Group Th (n = 87). However, only 61.1% (44/72) of cows in Group TI (n = 72) and 62.5% (55/88) of cows in Group C were in estrus and ovulated 46 and 52 ova altogether respectively. The ovulation rate (1.27 ± 0.03), calving rate (126.3%) and twinning rate (26.3%) in Group Th were greater than those in Groups Tl or C (Panimals (r = 0.7507, P<0.01). These results indicate that active immunization of postpartum cows against inhibin purified from porcine seminal plasma may increase the ovulation rate and induce twinning, suggesting the potential to develop a method to improve fertility in cows.

关键词： beef cows Seminal inhibin Active immunization Twinning Ovulation rate

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A Comparison of Methods to predict genomic breeding values of beef cattle based on genomic information

A Comparison of Methods to predict genomic breeding values o...

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中国畜牧兽医学会养牛学分会2011年学术研讨会

作者： Meng Zhang,Junya Li,Zhengkui Zhou, Huijiang Gao,Xue Gao,Shangzhong XU (Institute of animal science,beef cattle research center,Chinese Academy of Agricultural sciences, Key Laboratory of Farm animal Genetic Resources and Utilization of Ministry Agriculture,Beijing 100193,China)

INTRODUCTION Traditional genetic evaluation has successfully applied to beef cattle breeding in last decades. Henderson’s best linear unbiased prediction has been used as the statistical method for the estimated breeding value(VanRaden and Wiggans.1991).The availability of high throughput genotyping technologies and development of the computer technology have enabled more accurate genetic prediction using the massive genomic *** proposed the concept of Genomic selection(Meuwissen et al.

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Association of Polymorphisms in the Pit-1 Intron 5 with Growth Traits in Chinese cattle

Association of Polymorphisms in the Pit-1 Intron 5 with Grow...

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第十次全国畜禽遗传标记研讨会

作者： YANG Dong-ying, CHEN Hong, LAN Xian-yong, ZHANG Zheng-feng, TANG Li-gang, ZHANG Liang-zhi, WANG Xin-zhuang, WANG Yi-min, NIU Hu (college of animal science and technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China The research center of beef cattle Engineering and technology in Henan, Zhengzhou, Henan 450003, China)

The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to detecte polymorphism in the Pit-1 gene including intron 5. One single nucleotide polymorphism was found in intron 5 of Pit-1. The frequencies of alleles A/B in Nanyang cattle(NY), Qinchuan cattle(QC). Jiaxian Red cattle(JXR), Xizhen cattle(XZ), Luxi cattle(LX) and Holstein cow(H) populations were 0.444/0.556 , 0.477/0.523, 0.538/0.462, 0.421/0.579, 0.523/0.477, 0.475/0.525 respectively. Associations of the polymorphisms with growth traits in Nanyang cattle were analyzed using a general linear model procedure. The following parameters were greater in individuals with genotype AA than with genotype AB: body weight, average daily gain, body height, chest girth at 6, 12, 18 and 24 months(P allele A in other age groups. Therefore, genotype BB maybe a dominant genotype and allele B may be a dominant allele. These results imply that allele B of Pit-1 gene likely positively affects growth traits.

关键词： beef cattle Growth traits Pit intron 5 Polymorphism

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Effect of processing dejuiced sweet sorghum residues on ruminal fermentation characteristics in vitro

Effect of processing dejuiced sweet sorghum residues on rumi...

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The Ⅶ International Symposium on the Nutrition of Herbivores(ISNH-7)

作者： W Du~1 L.Wan~1 C.M.Sun~1 H.Song~2 Q.X.Meng~(1,3) ~1college of animal science and technology and beef cattle research Centre,China Agricultural University Beijing 100094,P.R.China ~2Nonghua Economy and Trade Company Beijing 100026,PR.China

The objective of this study was to examine the effect of processing dejuiced sweet sorghum residues on ruminal fermentation characteristics in *** sweet sorghum residues(DSSR) were subjected to three treatments:*** ensilage(E),*** of distillers after distillation of DSSR(ED),and *** distillers after distillation of DSSR(DD).After fermentation,E and ED treatments both had perfect silage fermentation *** the three treatments,E had the highest gas production,in vitro DM and NDF digestibilities,followed by ED and *** treatment had no significant effect(P>0.05)on in vitro NDF digestibility,the concentration of NH-N, total VFA,and individual VFA molar proportions,except for the molar proportions of acetic acid and butyric acid(P<0.01).The results indicate that treatment of dejuiced sweet sorghum residues with directly ensiling or ensiling distillers DSSR can improve their nutritive value.

关键词： sweet sorghum residues in vitro rumen fermentation gas production

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The polymorphisms of bovine VEGF gene and their associations with cattle growth traits

The polymorphisms of bovine VEGF gene and their associations...

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中国畜牧兽医学会第七届养牛学分会2009年学术研讨会

作者： Yonghong Pang~1,Chuzhao Lei~1,Chunlei Zhang~2,Xianyong Lan~1,Wangping Yue~2, Chuanwen Gu~2,Danxia Chen~2 and Juqiang Wang~3,Hong Chen~(1*) (1.college of animal science and technology,Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling,Shaanxi 712100,China 2.Institute of Cellular and Molecular Biology,Xuzhou Normal University, Xuzhou,Jiangsu 221116,China 3.research center of cattle Engineering technology in Henan Zhengzhou,Henan 450003,China)

＜正＞Introduction Vascular endothelial growth factor（VEGF） is a key regulator of vascular development in physiological and pathological conditions,with crucial roles in developmental blood vessel formation and hypoxia-induced tissue *** findings demonstrate that VEGF is an essential survival factor for muscle capillarity,bone formation,bone healing and regulation of ***,it may be a potential candidate

关键词： Chinese cattle VEGF gene Single nucleotide polymorphism(SNP)

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牛源A型多杀性巴氏杆菌分离鉴定及毒力基因检测

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黑龙江八一农垦大学学报 2019年第3期31卷 38-44页

作者：何泊宁赫鸣睿张世勋赵尚琪王天王丽吴陈华黄雯静刘宇岳山刘珊珊朱战波黑龙江八一农垦大学动物科技学院黑龙江省牛病防控工程技术研究中心黑龙江省兽医科学研究所

为了确定黑龙江省两个规模化奶牛场犊牛急性呼吸道感染的病因,进行了病原的分离纯化,细菌16S rDNA基因和多杀性巴氏杆菌特异性基因OmpH鉴定、生化试验、药敏试验、半数致死量试验、荚膜血清型分型及22种毒力因子的检测,确定主要病原的... 详细信息

为了确定黑龙江省两个规模化奶牛场犊牛急性呼吸道感染的病因,进行了病原的分离纯化,细菌16S rDNA基因和多杀性巴氏杆菌特异性基因OmpH鉴定、生化试验、药敏试验、半数致死量试验、荚膜血清型分型及22种毒力因子的检测,确定主要病原的种类、型别及生物学特性。结果表明,从死亡牛肺脏和患病牛鼻拭子分离的两株病原菌均为荚膜A型多杀性巴氏杆菌,分别被命名为WC-16551和LY-01,序列分析结果表明其与牛源多杀性巴氏杆菌同源性达到99%以上,药敏试验显示两株菌均对恩诺沙星、环丙沙星高度敏感,半数致死量结果表明LY-01菌株的毒力明显强于WC-16551菌株,两株分离菌均携带19种毒力因子,未检出hgbB、toxA和nanB三种基因。研究表明导致两个牛场发病的主要病原菌为荚膜血清型A型的多杀性巴氏杆菌。

关键词：牛 A型多杀性巴氏杆菌荚膜血清型分型毒力因子

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利用淘汰奶牛卵巢生产体外受精胚胎

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上海交通大学学报（农业科学版） 2003年第2期21卷 91-93,105页

作者：张德福王英张忠明韩增生陈晓羽吕俊张军民徐直上海市农业科学院畜牧兽医研究所上海201106 西北农业科技大学动物科技学院杨陵712100 上海奶牛育种中心上海200025 天津市农业科学院畜牧兽医研究所天津300112

以淘汰奶牛卵巢为材料,研究了卵巢采集方法、卵丘细胞和颗粒细胞对卵母细胞体外受精后发育的影响。结果表明奶牛屠宰后取其卵巢,用剖解法(平均9.1枚/卵巢),可比抽吸法(平均6.5枚/卵巢)得到更多的可用卵母细胞(p<0.01)。周围无卵丘细胞... 详细信息

以淘汰奶牛卵巢为材料,研究了卵巢采集方法、卵丘细胞和颗粒细胞对卵母细胞体外受精后发育的影响。结果表明奶牛屠宰后取其卵巢,用剖解法(平均9.1枚/卵巢),可比抽吸法(平均6.5枚/卵巢)得到更多的可用卵母细胞(p<0.01)。周围无卵丘细胞包围的裸露卵母细胞经成熟培养和体外受精后,卵裂率为41.8%,但仅27.5%停留在8～16-细胞期,最高发育阶段为16-细胞期;有3层以上卵丘细胞紧密包围的卵丘-卵母细胞复合体(Cumulus-OocyteComplex,COC)经成熟培养和体外受精后,卵裂率(64.5%VS54.7%,p<0.01)和囊胚发育率(34.0%VS18.2%,p<0.01)显著高于卵丘细胞包被不全的卵母细胞。在培养液中添加颗粒细胞虽然没有促进牛受精卵的卵裂率,但提高了囊胚率及其细胞数(p<0.01).

关键词：淘汰奶牛卵巢体外受精胚胎卵丘细胞颗粒细胞

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THE DEVELOPMENT AND DESIGN OF DAIRY cattle EXPERT DECISION-MAKING SYSTEM （DCEDS）

THE DEVELOPMENT AND DESIGN OF DAIRY CATTLE EXPERT DECISION-M...

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Progress of Information technology in Agriculture

作者： Zhang Xuewei Song Zhiwen Song Guimin Lu Wenlong Ma Wenzhi Zhao Xianjian Zhang YiTianjin Agricultural college Post Code: 300384 Tianjin China Institution of Tianjin Agricultural science Post Code: 300192 Tianjin China

＜正＞ On the basis of the characteristic description and requirement of dairy production, by using the powerful technique performance of PAID 3.0, with the help of the new technique of network, such as uncertainty-reasoning, multimedia, friendly human-machine cooperation, the Dairy cattle Expert Decision-Making System （ DCEDS ） has been developed. The functions of the system are to spread the knowledge consultation and technological decision-making of dairy production for local enterprise users and to speed the development of dairy production in our city even in the whole country.

关键词： PAID3.0 dairy production expert system decision-making consultation

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