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The Reliability of Plasma Exosome Concentrations in Healthy Male Individuals

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Journal of Health Science 2017年第2期5卷 81-94页

作者： Eman Abu-seer Oxford Brookes University Gipsy Lane Oxford OX3 0BP UK

Exosomes are nanometer sized vesicles （30-100 nm）, released from cells following the fusion of multivesicular bodies with the plasma membrane. Exosomes have a major role in intracellular communication, immune response modulation, physiological and pathological functions, and an important use in different drug therapies. Moreover, they have been identified as potential biomarkers and are involved in cancer and many other disease processes. Despite the developments in this area, no studies exist that assess the variability of plasma exosomes measures within and between subjects. The purpose of this study was to assess within and between day variability of plasma exosomes concentration in healthy individuals. Also, the stability of exosomes during freeze/thawing cycles was measured in this study. Eleven healthy men were assessed for reliability of exosome concentrations taken over the same sampling period, over 10 hours of a day and over five days. Exosomes were isolated by differential ultracentrifugation and characterized by their size distribution and morphology by electron microscopy and using the Nanosight tracking analysis respectively. Mean plasma exosome values werel.505-2.245 x 10^8/mL）. Within day variability was not significantly different （P = 0.95）, and between day variability was not significantly different （P = 0.42）. The interclass correlation coefficients （ICC） of 0.80 for within sample showed good reliability; ICC of 0.84 for between day plasma exosomes concentrations showed good reliability, and the within day ICC of 0.70 showed a moderate reliability. There was no significant difference observed in using fresh or frozen plasma in exosome quantification or exosome concentration. The statistics show that there is little significant variability in plasma exosome concentrations taken over a single sampling period, over a day or between days, which is vital for plasma exosome studies.

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菜鸟版Exploit编写指南之四十七：The短er， the better——精简你的数字字母ShellCode

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黑客防线 2008年第8期 44-49页

作者： seer

新东方戴云教主尝曰：“The长er，the better”，是乃应试写作之要义。然shellcode之编写则反之，故曰：“The短er，thebetter”。

关键词：溢出 Sehllcode 算法

后SQL注入时代的饕餮盛宴——_动易网站管理系统***注入漏洞分析与利用

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黑客防线 2007年第12期 20-22页

作者： seer

欢呼吧，入侵者们．新的漏洞已经横空出世．收割的时节即将来临。

关键词：网站管理系统 SQL注入漏洞分析入侵者

菜鸟版Exploit编写指南之五十：Thinking in MS09-002——IE7内存破坏漏洞原理分析

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黑客防线 2009年第4期 57-61页

作者： SAI seer

2月11日，微软发布了等级为“严重”的MS09-002漏洞的安全更新程序。于是，又一个老少皆宜，雅俗共赏，居家旅行必备的挂马漏洞诞生了。在公开后的短时间内，MS09-002便一跃为目前危害最严重的漏洞，大有取代去年的XML漏洞之势。

关键词：漏洞 ShellCode MS09—002

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微软MPEG-2栈溢出漏洞分析手记

黑客防线 2009年第8期 14-17页

作者： --seer SAI

DirectShow 0Day第二波爆发!7月5日，针对微软MPEG－2视频解析模块0Day漏洞的网马开始大规模“上架”，该漏洞利用了MPEG－2视频组件注册的ActiVex控件***中的一个缓冲区溢出漏洞，可以覆盖到堆栈中的SEH指针。

关键词： MPEG-2 漏洞分析微软 MPEG-2视频 DirectShow 栈溢出 ActiVex控件缓冲区溢出漏洞

榨干Yahoo！的最后一滴血——Yahoo Widget ActiveX溢出漏洞利用

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黑客防线 2007年第9期 16-20页

作者： seer[L.S.T]＆[N.N.U]

各位溢出爱好者们．看文章标题．我们又有的玩了。从Yahoo！Messager那几个ActiveX溢出被发现开始．Yahoo！就一直是漏洞大户。而现在．终于轮到了Yahoo！Widget了。

关键词： Yahoo 溢出漏洞 ActiveX Widget 文章标题

菜鸟版Exploit编写指南之四十八：MS08-078启示录——IE7 XML远程代码执行漏洞分析与利用

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黑客防线 2009年第1期 58-62页

作者： seer[N.N.U] ＆ SAI[N.N.U]

微软2008年末巨献，MS08—078XML远程代码执行漏洞在12月震撼登场。朋友们enjoy hacking !

关键词：漏洞 ShellCode XML MS08-078

APPLICATION OF THE DC RESISTIVITY METHODS TO THE STUDY OF GROUNWATER IN THE WESTERN PART OF AMMAN BASIN, JORDAN

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Scientia Geologica Sinica 2001年第4期 244-255页

作者： Abdallah S. Al- Zoubi and Atef Kharabsheh Al- Balqa Applied University Al- Salt, Jordan Address: Amman 11844, Bayder Wadi El- seer, P.O. Box 142237, Jordan.

Electrical resistivity measurements by utilizing the Schlumberger array were accomplished at the Western Amman Catchment Basin in Jordan. The objective of this work was to study the groundwater potential in order to clarify the subsurface geology of the study area, and finally to locate productive borehole sites. Thirteen vertical Electrical sounding (VES) were conducted by IRIS resistivity instrument in 1999. The AB/2 spacing for VES is 500m. The VES curves obtained were interpreted by using master curves and software modeling techniques. Results of the quantitative interpretation indicate that there are four groups of resistivity values in the study area, corresponding to four geological layers. Besides, two subsurface faults were also detected.

关键词： VES JORDAN APPLICATION OF THE DC RESISTIVITY METHODS TO THE STUDY OF GROUNWATER IN THE WESTERN PART OF AMMAN BASIN DC

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互联网天地 2006年第12期 61-61页

作者： seer

2001年春节从老家回到北京，我的毛裤里是母亲仔细缝着的2000块钱，也就是我一个学期的生活费。钱虽然不少，但是那个时候刚到北京半年多，还没见过什么世面，看到很多新鲜好玩的东西，什么超薄的Sony Walkman、复读机啦、Addis很奇怪... 详细信息

2001年春节从老家回到北京，我的毛裤里是母亲仔细缝着的2000块钱，也就是我一个学期的生活费。钱虽然不少，但是那个时候刚到北京半年多，还没见过什么世面，看到很多新鲜好玩的东西，什么超薄的Sony Walkman、复读机啦、Addis很奇怪的篮球鞋啦。

关键词： Walkman Sony 复读机篮球鞋北京超薄