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Integrated tool for microsatellite isolation and validation from the reference genome and their application in the study of breeding turnover in an endangered avian population

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Integrative Zoology 2018年第5期13卷 553-568页

作者： Xian HOU Pengwei XU Zhenzhen LIN Josephine d’URBAN-JACKSON AndrewdIXON Batbayar BOLd Jiliang XU Xiangjiang ZHAN Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijingChina School of Nature Conservation Beijing Forestry UniversityBeijingChina BGI-Tech BGI-ShenzhenShenzhenChina Milner Centre for Evolution Department of Biology and BiochemistryUniversity of BathBathUK Emirates Falconers’Club Abu DhabiUnited Arab Emirates Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research BeijingChina Wildlife Science and Conservation Center UlaanbaatarMongolia Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingChina

Accurate individual identification is required to estimate survival rates in avian *** endangered species,non-invasive methods of obtaining individual identification,such as using molted feathers as a source of dNA for microsatellite markers,are preferred because of less disturbance,easy sample preparation and high *** the availability of many avian genomes,a few pipelines isolating genome-wide microsatellites have been published,but it is still a challenge to isolate microsatellites from the reference genome ***,we have developed an integrated tool comprising a bioinformatic pipeline and experimental procedures for microsatellite isolation and validation based on the reference *** have identified over 95000 microsatellite loci and established a system comprising 10 highly polymorphic markers(PIC value:0.49–0.93,mean:0.79)for an endangered species,saker falcon(Falco cherrug).These markers(except 1)were successfully amplified in 126 molted feathers,exhibiting high amplification success rates(83.9–99.7%),high quality index(0.90–0.97)and low allelic dropout rates(1–9.5%).To further assess the efficiency of this marker system in a population study,we identified individual sakers using these molted feathers(adult)and 146 plucked feathers(offspring).The use of parent and offspring samples enabled us to infer the genotype of missing samples(N=28),and all adult genotypes were used to ascertain that breeding turnover is a useful proxy for survival estimation in *** study presents a cost-effective tool for microsatellite isolation based on publicly available reference genomes and demonstrates the power of this tool in estimating key parameters of avian population dynamics.

关键词： breeding turnover Falco cherrug feather microsatellite isolation parentage analysis reference genome saker falcon

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A review of reflux esophagitis around the world

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World Journal of Gastroenterology 1998年第4期4卷 8-12页

作者： Edwin J. Zarling, M.d.

ＡｒｅｖｉｅｗｏｆｒｅｆｌｕｘｅｓｏｐｈａｇｉｔｉｓａｒｏｕｎｄｔｈｅｗｏｒｌｄＥｄｗｉｎＪ．Ｚａｒｌｉｎｇ，Ｍ．Ｄ．Ｓｕｂｊｅｃｔｓｈｅａｄｉｎｇｓｇａｓｔｒｏｅｓｏｐｈａｇｅａｌｒｅｆｌｕｘ／ｅｐｉｄｅｍｉｏｌｏ... 详细信息

ＡｒｅｖｉｅｗｏｆｒｅｆｌｕｘｅｓｏｐｈａｇｉｔｉｓａｒｏｕｎｄｔｈｅｗｏｒｌｄＥｄｗｉｎＪ．Ｚａｒｌｉｎｇ，Ｍ．Ｄ．Ｓｕｂｊｅｃｔｓｈｅａｄｉｎｇｓｇａｓｔｒｏｅｓｏｐｈａｇｅａｌｒｅｆｌｕｘ／ｅｐｉｄｅｍｉｏｌｏｇｙ；ｇａｓｔｒｏｅｓｏｐｈａｇｅ...

关键词： gastroesophageal reflux/epidemiology gastroesophageal reflux/pathophysiology gastroesophageal reflux/complications gastroesophageal reflux/diagnosis gastroesophageal reflux/therapy

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俄罗斯堪察加中部Baranevskoy金-银矿床矿物学与流体包裹体研究

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地学前缘 2020年第5期27卷 136-150页

作者： Victor M.Okrugin Elena d.SKilskaia Institute of Volcanology and Seismology Far Eastern Branch of Russian Academy of SciencesPiip Boulevard9Petropavlovsk-Kamchatsky 683006Russia

Baranevskoy金-银矿床产于巴尔喀什火山的火山口,该火山坐落在堪察加中部矿区东南部。本文基于矿物学原理和流体包裹体数据分析探讨了Baranevskoy金-银矿床的成矿环境及其物理化学条件。Baranevskoy金-银矿床的围岩为中新世—上新世的... 详细信息

Baranevskoy金-银矿床产于巴尔喀什火山的火山口,该火山坐落在堪察加中部矿区东南部。本文基于矿物学原理和流体包裹体数据分析探讨了Baranevskoy金-银矿床的成矿环境及其物理化学条件。Baranevskoy金-银矿床的围岩为中新世—上新世的安山岩和玄武岩。热液蚀变活动随深度逐渐变化,从而可以进一步划分出最深部的石英带、中部的石英-绢云母(明矾石)-黄铁矿-铁钛氧化物带及其伴生的石英-绢云母-伊利石-黄铁矿矿物组合和浅部的石英-冰长石-水云母-黏土矿物-碳酸盐岩带。成矿早期存在密集浸染的铜矿化,主要矿石矿物有黄铜矿、斑铜矿、砷黝铜矿-黝铜矿,并在Rhzavaya矿脉中存在少量的自然金。其中砷黝铜矿-黝铜矿系列以砷黝铜矿和黝铜矿两个端员作为代表,且以黝铜矿为主。成矿后期产出代表晚期金-银矿化的自然金、黄铁矿、黄铜矿、闪锌矿、方铅矿、碲化物和硫酸盐等标志性矿物。早期铜矿化(第一期)被认为是中硫阶段,紧随其后的为低硫型金-银矿化(第二期和第三期)。金从第一期到第三期都有沉淀。经研究发现,自然金也赋存于变质围岩的岩石裂隙内。早期的自然金相对富银,其中金的摩尔分数为59%~65%,低于后期(第二、第三期)自然金中金的摩尔分数(64%~72%)。流体包裹体显微测温结果显示,位于中部(Central)矿脉的包裹体均一温度为190~280℃,Rzhavaya矿脉的包裹体为190~240℃,产出自然金的蚀变围岩中石英的包裹体温度为230~310℃。包裹体总体表现出低盐度(0.9%~2.4%NaCleq)特征,推测存在大气水的混入。

关键词：金矿床火山弧斑铜矿气液包裹体堪察加

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Characterization of mechanical properties and microstructures of spark plasma sintered and cryo-rolled AA2024−Y composites

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Transactions of Nonferrous Metals Society of China 2020年第6期30卷 1439-1451页

作者： CH.S.VIdYASAGAR d.B.KARUNAKAR Metallurgical and Materials Engineering department Indian Institute of TechnologyRoorkee-247667India Mechanical and Industrial Engineering department Indian Institute of TechnologyRoorkee-247667India

The relationship between the microstructure and mechanical properties of the spark plasma sintered AA2024 Y composites subjected to cryo-rolling was investigated. Yttrium addition enhances the mechanical properties of the composites by promoting grain refinement and precipitation. However, there is a clear trend of initial increase and later decrease in the properties. Also, it is observed that 0.3 wt.% of yttrium is the optimum amount of reinforcement content to obtain the highest mechanical properties. To further improve the tensile strength of the composites, cryo-rolling was performed on the composites under standard cryogenic conditions by several passes up to a reduction of 25%. The mechanical properties and the corresponding microstructures of composites after cryo-rolling were correlated. The SEM and TEM microstructures reveal that the samples exhibit dual size grains, i.e., nanograins are formed as sub-grains within the actual grain. due to the grain size reduction and the increase in the dislocation density, the tensile properties are remarkably improved compared to those of the composites before cryo-rolling. The highest mechanical properties like hardness, YS and UTS are found to be 153 HV, 539 MPa and 572 MPa, respectively, with a reasonable ductility in the composite with 0.3 wt.% Y.

关键词： secondary processing cryo-rolling nanograins yttrium addition dual size grains spark plasma sintering

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Corrosion inhibition effect of N, N′-bis(2-pyridylmethylidene)-1,2-diiminoethane on az91d magnesium alloy in acidic media

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Transactions of Nonferrous Metals Society of China 2014年第11期24卷 3441-3451页

作者： d.SEIFZAdEH A.BEZAATPOUR R.ASAdPOUR JOGHANI Physical Chemistry Research Laboratory Applied Chemistry Department Faculty of ScienceUniversity of Mohaghegh Ardabili Inorganic Chemistry Research Laboratory Applied Chemistry Department Faculty of Science University of Mohaghegh Ardabili

The inhibition effect of electrochemical noise, EIS and surface analysis to evaluate N'-bis (2-pyridylmethylidene)- 1,2-diiminoethane (BPIE) Schiff base against az91d alloy corrosion in 0.01 mol/L HCl was investigated by different electrochemical methods. Potentiodynamic polarization curves revealed that the BPIE acts as a mixed-type corrosion inhibitor. Electrochemical impedance spectroscopy (EIS) measurements confirmed the corrosion inhibition effect of the BPIE. As the inhibitor concentration increased, the charge transfer resistance increased and the double layer capacitance decreased due to more inhibitor adsorption on the surface. The results obtained by analysis of electrochemical noise (EN) data in time and frequency domains are in good agreement with EIS and polarization results. Moreover, scanning electron microscopy (SEM), X-ray diffraction (XRd) and energy dispersive X-ray (EdX) were used to investigate the corrosion inhibition of the BPIE. SEM images showed that the corrosion damage of the alloy surface reduced in the presence of BPIE. The intensity of the XRd peaks corresponding to magnesium-rich α phase increased in the presence of BPIE, indicating lower corrosion of alloy sample. Also, EdX analysis approved the corrosion inhibition performance of the BPIE. The studied Schiff base compound acts by physical adsorption on the alloy surface and its adsorption obeys the Langmuir isotherm.

关键词： az91d magnesium alloy corrosion acidic media inhibition Schiff base

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Microbial Activity and Culturable Bacterial diversity in Sediments of the Great Rann of Kachchh,Western India

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Pedosphere 2014年第1期24卷 45-55页

作者： G.SUBRAHMANYAM N.KHONdE d.M.MAURYA L.S.CHAMYAL G.ARCHANA department of Microbiology and Biotechnology Centre Faculty of ScienceThe Maharaja Sayajirao University of Baroda department of Geology Faculty of ScienceThe Maharaja Sayajirao University of Baroda

The Great Rann of Kachchh, a vast expanse of salt desert in Western India is a unique hostile ecosystem posing an extreme environment to life forms due to high salt content, hyper-axid climate, seasonal water logging and extremes of temperature. In the virtual absence of natural vegetation, soils and sediments of Rann of Kachchh axe microbially dominated ecosystems. In the present study microbial activity and the diversity of cultivated heterotrophic bacteria were investigated in the sediments collected along a 5-m exposed section at Khadir Island in the Great Rann of Kachchh. Microbial activity （as an index of sediment enzymes） was found to be high in the middle of the section （200-280 cm）. dehydrogenase （dHA）, substrate-induced dHA and alkaline phosphatase activities revealed the oligotrophic nature of the basal portion （320-480 cm）. Abundant bacterial isolates obtained from different depths were found to be clustered in 12 different phylogenetic groups by amplified ribosomal dNA restriction analysis. 16S rRNA gene sequencing revealed the dominant bacterial ribotypes to be affiliated to Firmicutes （Families Bacillaceae and Staphyloeoccaeeae） and Aetinobaeteria （Family Brevibaeteriaceae） with minor contribution of Proteobacteria （Families Phyllobacteriaeeae and Bartonellaceae）, pointing their endurance and adaptability to environmental stresses. Statistical analysis indicated that sediment organic carbon, salinity, total available nitrogen and total available phosphorous are most likely critical determinants of microbial activity in the Khadir Island sediments.

关键词： amplified ribosomal dNA restriction analysis enzymes extreme environment heterotrophic bacteria 16S rRNA gene sequencing

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Performance of engineered cementitious composite containing stone slurry powder waste

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Journal of Central South University 2021年第7期28卷 1990-2002页

作者： Maninder SINGH Babita SAINI H.d.CHALAK department of Civil Engineering National Institute of Technology KurukshetraHaryana 136119India

Most of the materials used in engineered cementitious composite are fine in size to achieve ductile *** slurry powder(SSP)is an inert material obtained from stone industries as by-product which may cause hazardous impact on *** this research work,partial replacement of silica sand(SS)and fine sand(FS)by SSP with different contents(25%and 50%each)for making engineered cementitious composite has been *** performance was evaluated on the basis of strength,tensile strain,mid span deflection capacity,ultra-sonic pulse velocity and *** strength was found to be increased at 25%SSP in both replacements;whereas,strength decreased slightly at 50%*** strain,mid span deflection and quality of concrete were enhanced with increase in SSP *** SSP formed denser cementitious composite can help to save the natural resources and contribute in making green cementitious composite.

关键词： stone slurry powder fine sand scanning electron microscopy energy dispersive X-ray spectroscopy ultrasonic pulse velocity

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3d Monte-Carlo model to study the transport of hot electrons in the context of inertial confinement fusion. Part Ⅱ

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Matter and Radiation at Extremes 2022年第6期7卷 64-75页

作者： A.Tentori A.Colaïtis d.Batani Centre Lasers Intenses et Applications CELIAUniversitéBordeaux CEA-CNRSUMR 5107F-33405 TalenceFrance

We describe two numerical investigations performed using a 3d plasma Monte-Carlo code,developed to study hot-electron transport in the context of inertial confinement *** code simulates the propagation of hot electrons in ionized targets,using appropriate scattering differential cross sections with free plasma electrons and ionized or partially ionized *** this paper,we show that a target in the plasma state stops and diffuses electrons more effectively than a cold target(i.e.,a target under standard conditions in which ionization is absent).This is related to the fact that in a plasma,the nuclear potential of plasma nuclei has a greater range than in the cold case,where the screening distance is determined by the electronic structure of ***,in the ablation zone created by laser interaction,electrons undergo less severe scattering,counterbalancing the enhanced diffusion that occurs in the *** also show that hard collisions,i.e.,collisions with large polar scattering angle,play a primary role in electron beam diffusion and should not be *** application of the plasma MonteCarlo model to typical shock ignition implosions suggests that hot electrons will not give rise to any preheating concerns if their Maxwellian temperature is lower than 25–30 keV,although the presence of populations at higher temperatures must be *** result does not depend strongly on the initial angular divergence of the electron beam set in the simulations.

关键词： electrons diffusion neglected

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GPS资料获取的四川地区应变率演化状态

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西南交通大学学报 2018年第2期53卷 344-350页

作者：徐锐 d. Sarah.Stamps 黄圣睦四川省地震局四川成都610031 弗吉尼亚理工大学地球科学学院美国弗吉尼亚24061

为了研究汶川5·12地震事件对青藏高原东南缘及四川盆地的应力再分配作用及该区域现今的地震危险性,使用1997—2015年共计355个GPS站点观测资料获取了该区域1997—2008、2009—2011、2011—2013、2013—2015年共计4期独立的速度场,基于... 详细信息

为了研究汶川5·12地震事件对青藏高原东南缘及四川盆地的应力再分配作用及该区域现今的地震危险性,使用1997—2015年共计355个GPS站点观测资料获取了该区域1997—2008、2009—2011、2011—2013、2013—2015年共计4期独立的速度场,基于规则格网和张力样条插值方法计算了上述速度结果对应的面膨胀率、垂直轴旋转率及最大剪应变率.以2008年汶川Ms8.0级地震为主要节点,以芦山2013年Ms7.0级地震、康定2014年Ms6.3级地震为参考节点,分析了应变率场的时空演化过程及其与地震事件的潜在关联性.研究结果显示:随着汶川震后的时间推移,沿龙门山断裂的3种应变率分布均有减弱趋势,但至今仍未恢复至震前状态,说明该区域的震后回弹受下地壳黏弹性流变物质的弛豫影响较为显著;安宁河—则木河断裂及周边的面压缩率在汶川地震之后有增强趋势,该趋势可能与区域的地震危险性有所关联;安宁河—则木河断裂带及鲜水河断裂北段是未来一段时间该研究区域内应该持续关注的主要地震危险区.

关键词： GPS 速度场应变率演化地震危险性

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FORM FACTORS FOR RAdIATIVE PION ANd KAON dECAYS

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Chinese Physics C 2016年第10期40卷 685-686页

作者： M. Bychkov G. d'Ambrosio University of Virginia INFN Sezione di Napoli

The radiative decays, π^± → l^±vγ and K^± →l^±vγ, with 1 standing for an e or a μ, and γ for a real or virtual photon （e＋e- pair）, provide a powerful tool to investigate the hadronic structure of pions and kaons. The structuredependent part Sdi of the amplitude describes the emission of photons from virtual hadronic states, and is parametrized in terms of form factors V,A, （vector, axial vector）,

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