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MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device

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Microsystems & Nanoengineering 2020年第1期6卷 145-157页

作者： Yinnian Feng Adam K.White jamin B.Hein Eric A.Appel Polly M.Fordyce Department of Genetics Stanford UniversityStanfordCA 94305USA Department of Bioengineering Stanford UniversityStanfordCA 94305USA Department of Biology Stanford UniversityStanfordCA 94305USA The Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical SciencesUniversity of CopenhagenBlegdamsvej 3b2200 CopenhagenDenmark Department of Materials Science&Engineering Stanford UniversityStanfordCA 94305USA Stanford ChEM-H Stanford UniversityStanfordCA 94305USA Chan Zuckerberg Biohub San FranciscoCA 94110USA

The widespread adoption of bead-based multiplexed bioassays requires the ability to easily synthesize encoded microspheres and conjugate analytes of interest to their ***,we present a simple method(MRBLEs 2.0)for the efficient high-throughput generation of microspheres with ratiometric barcode lanthanide encoding(MRBLEs)that bear functional groups for downstream surface *** production in MRBLEs 2.0 relies on the manual mixing of lanthanide/polymer mixtures(each of which comprises a unique spectral code)followed by droplet generation using single-layer,parallel flow-focusing devices and the off-chip batch polymerization of droplets into *** streamline downstream analyte coupling,MRBLEs 2.0 crosslinks copolymers bearing functional groups on the bead surface during bead *** the MRBLEs 2.0 pipeline,we generate monodisperse MRBLEs containing 48 distinct well-resolved spectral codes with high throughput(>150,000/min and can be boosted to 450,000/min).We further demonstrate the efficient conjugation of oligonucleotides and entire proteins to carboxyl MRBLEs and of biotin to amino ***,we show that MRBLEs can also be magnetized via the simultaneous incorporation of magnetic nanoparticles with only a minor decrease in the potential code *** the advantages of dramatically simplified device fabrication,elimination of the need for custom-made equipment,and the ability to produce spectrally and magnetically encoded beads with direct surface functionalization with high throughput,MRBLEs 2.0 can be directly applied by many labs towards a wide variety of downstream assays,from basic biology to diagnostics and other translational research.

关键词： beads spectral functional

DCAF1 (VprBP): emerging physiological roles for a unique dual-service E3 ubiquitin ligase substrate receptor

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journal of Molecular Cell Biology 2019年第9期11卷 725-735页

作者： N.Max Schabla Koushik Mondal Patrick C.swanson Department of Medical Microbiology and Immunology Creighton University2500 California PlazaOmahaNE 68178USA Present address:Institute of Cellular and Molecular Immunology Georg-August-University of GottingenMedical FacultyHumboldtallee 3437073 GottingenGermany Present address:Department of Ophthalmology Hamilton Eye InstituteUniversity of Tennessee Health Sciences Center930 Madison AvenueMemphisTN 38163USA

Cullin-RING ligases(CRLs)comprise a large group of modular eukaryotic E3 ubiquitin *** this family,the CRL4 ligase(consisting of the Cullin4[CUL4]scaffold protein,the Rbxl RING finger domain protein,the DNA damage-binding protein 1[DDB1],and one of many DDBl-associated substrate receptor proteins)has been intensively studied in recent years due to its involvement in regulating various cellular processes,its role in cancer development and progression,and its subversion by viral accessory *** discovered as a target for hijacking by the human immunodeficiency virus accessory protein r,the normal targets and function of the CRL4 substrate receptor protein DDBl-Cul4-associated factor 1(DCAF1;also known as VprBP)had remained elusive,but newer studies have begun to shed light on these ***,we review recent progress in understanding the diverse physiological roles of this DCAF1 in supporting various general and cell type-specific cellular processes in its context with the CRL4 E3 ligase,as well as another HECT-type E3 ligase with which DCAF1 also associates,called EDD/*** also discuss emerging questions and areas of future study to uncover the dynamic roles of DCAF1 in normal physiology.

关键词： V(D)j recombination Merlin Dicer p53 EDD/DYRK2 TET Hippo

Generation of strong magnetic fields for magnetized plasma experiments at the 1-MA pulsed power machine

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Matter and Radiation at Extremes 2021年第4期6卷 31-42页

作者： V.V.Ivanov A.V.Maximov R.Betti L.S.Leal j.D.Moody K.j.swanson N.A.Huerta Department of Physics University of NevadaRenoNevada 89557USA Laboratory for Laser Energetics University of RochesterRochesterNew York 14623USA Department of Physics and Astronomy University of RochesterRochesterNew York 14623USA Lawrence Livermore National Laboratory LivermoreCalifornia 94550USA

Pulsed power technology provides a platform for investigating plasmas in strong magnetic fields using a university-scale *** here are methods for generating and measuring the 1–4-MG magnetic fields developed for the 1-MA Zebra pulsed power generator at the University of Nevada,Reno.A laser coupled with the Zebra generator produces a magnetized plasma,and experiments investigate how a megagauss magnetic field affects the two-plasmon decay and the expansion of the laser-produced plasma in both transverse and longitudinal magnetic fields.

关键词： magnetic generator fields

Sodium-glucose cotransporter-2 inhibitor use in kidney transplant recipients

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World journal of Transplantation 2023年第5期13卷 239-249页

作者： Pavithra Ramakrishnan Neetika Garg Samantha Pabich Didier A Mandelbrot Kurtis j swanson Department of Medicine University of MinnesotaMinneapolisMN 55455United States Department of Medicine University of Wisconsin School of Medicine and Public HealthMadisonWI 53705United States

Sodium-glucose cotransporter-2 inhibitors(SGLT2i)are novel oral hypoglycemic agents garnering much attention for their substantial *** recent data have positioned SGLT2i at the forefront of diabetic chronic kidney disease(CKD)and heart failure ***2i use post-kidney transplant is an emerging area of *** from this mini review include the following:Empagliflozin is the most prescribed SGLT2i in kidney transplant recipients(KTRs),median time from transplant to initiation was 3 years(range:0.88-9.6 years).Median baseline estimated glomerular filtration rate(eGFR)was 66.7 mL/min/1.73 m2(range:50.4-75.8).Median glycohemoglobin(HgbA1c)at initiation was 7.7%(range:6.9-9.3).SGLT2i were demonstrated to be effective short-term impacting HgbA1c,eGFR,hemoglobin/hematocrit,serum uric acid,and serum magnesium *** are shown to be safe in KTRs with low rates of infections,hypoglycemia,euglycemic diabetic ketoacidosis,and stable tacrolimus *** data is needed to demonstrate long-term ***2i appear to be safe,effective medications for select *** present literature,though limited,is founded on precedent robust research in CKD patients with *** research/utilization of SGLT2i is vital to not only identify long-term patient,graft and cardiovascular outcomes of these agents,but also to augment management in KTRs.

关键词： Sodium glucose cotransporter-2 Sodium glucose cotransporter-2 inhibitor Kidney transplantation Diabetes Post-transplant diabetes mellitus New onset diabetes after transplant

Post-transplant erythrocytosis after kidney transplantation:A review

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World journal of Transplantation 2021年第6期11卷 220-230页

作者： Beyann Alzoubi Abish Kharel Rushad Machhi Fahad Aziz Kurtis j swanson Sandesh Parajuli Department of Medicine University of Wisconsin School of Medicine and Public HealthUniversity of Wisconsin MadisonMadisonWI 53705United States

Post-transplant erythrocytosis(PTE)is defined as persistently elevated hemoglobin>17 g/dL or hematocrit levels>51%following kidney transplantation,independent of *** is a relatively common complication within 8 months to 24 months post-transplantation,occurring in 8%-15%of kidney transplant *** PTE risk factors include male gender,normal hemoglobin/hematocrit pre-transplant(suggestive of robust native kidney erythropoietin production),renal artery stenosis,patients with a well-functioning graft,and dialysis before *** factors play a role in the development of PTE,however,underlying endogenous erythropoietin secretion pre-and post-transplant is *** contributory factors include the renin-angiotensin-aldosterone system,insulin-like growth factors,endogenous androgens,and local renal *** patients with PTE experience mild symptoms like malaise,headache,fatigue,and *** prior investigations showed an increased risk of thromboembolic events,more recent evidence tells a different story-that PTE perhaps has lessened risk of thromboembolic events or negative graft outcomes than previously *** the evaluation of PTE,it is important to exclude other causes of erythrocytosis including malignancy before *** converting enzyme inhibitors(ACE-I)and angiotensin receptor blockers(ARBs)are the mainstays of *** ACE-I/ARB use has likely contributed to the falling incidence of *** this review article,we summarize the current literature in the field of post-transplant erythrocytosis after kidney transplantation.

关键词： Post-transplant erythrocytosis Kidney transplantation Epidemiology Treatment Guidelines Outcomes

MOCS2 links nucleotide metabolism to nucleoli function

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journal of Molecular Cell Biology 2021年第11期13卷 838-840页

作者： Tamaki Suganuma Selene K.swanson Madelaine Gogol Timothy j.Garrett Laurence Florens jerry L.Workman Workman Lab Stowers Institute for Medical ResearchKansas CityMO 64110USA Proteomics Stowers Institute for Medical ResearchKansas CityMO 64110USA Computational Biology Stowers Institute for Medical ResearchKansas CityMO 64110USA Department of Pathology Immunology and Laboratory MedicineUniversity of FloridaCollege of MedicineGainesvilleFL 32610USA

Dear Editor,Three polyamines,putrescine,spermidine,and spermine,are tightly regulated and are synthesized from arginine,ornithine,and methionine(Miller-Fleming et al.,2015;Figure 1A).Polyamine levels are increased in the red blood cells of amyotrophic lateral sclerosis and Parkinson’s disease(PD)patients(Miller-Fleming et al.,2015).Sulfur amino acid catabolism regulated by the molybdopterin synthase associating complex prevents onset of early events in PD and Alzheimer’s disease(Suganuma et al.,2018).MPT synthase,a heterodimer of MOCS2A and MOCS2B encoded by MOCS2,is essential for the biosynthesis of molybdenum cofactor(Moco).

关键词： al. metabolism Alzheimer

Atlas of minimally invasive thoracic surgery (VATS)

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Role of novel biomarkers in kidney transplantation

2011年

作者： Robert j. Mckenna jr Ali Mahtabifard Scott j. swanson.

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World journal of Transplantation 2020年第9期10卷 230-255页

作者： Kurtis j swanson Fahad Aziz Neetika Garg Maha Mohamed Didier Mandelbrot Arjang Djamali Sandesh Parajuli Department of Medicine University of Wisconsin School of Medicine and Public HealthMadisonWI 53705United States Department of Surgery University of Wisconsin School of Medicine and Public HealthMadisonWI 53705United States

Clinical application of biomarkers is an integral component of transplant *** and scientists alike are in search of better biomarkers than the current serologic(serum creatinine,donor-specific antibodies),urine-derived(urinalysis,urine protein),and histologic ones we now *** science behind recent biomarker discovery spans across multiple molecular biologic disciplines,including transcriptomics,proteomics,and *** methodology and integration of basic and clinical approaches have allowed researchers to unearth molecular phenomena preceding clinical *** can be classified in several *** this review,we have classified them via their origin and outcome:Primarily immunologic,i.e.,representative of immune regulation and dysfunction and non-immunologic,pertaining to delayed graft function,cardiovascular events/mortality,infection,malignancy,posttransplant diabetes,graft,and patient *** biomarker uses to guide the diagnosis and management of transplant-related outcomes is a promising area of ***,the use of biomarkers to predict outcomes after kidney transplantation is not well *** this review,we summarize the recent studies illustrating biomarker use and transplant outcomes.

关键词： Biomarkers Kidney Transplantation Rejection Infection Mortality Graft survival

Large-diameter trees dominate snag and surface biomass following reintroduced fire

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Ecological Processes 2020年第1期9卷 475-487页

作者： james A.Lutz Soren Struckman Tucker j.Furniss C.Alina Cansler Sara j.Germain Larissa *** Darren j.McAvoy Crystal A.Kolden Alistair M.S.Smith Mark E.swanson Andrew j.Larson Department of Wildland Resources S.J.and Jessie E.Quinney College of Natural ResourcesUtah State University5230 Old Main HillLoganUtah 84322-5230USA School of Environmental and Forest Sciences University of WashingtonBox 352100SeattleWashington 98195USA Management of Complex Systems University of CaliforniaMerced5200 North Lake RoadMercedCalifornia 95343USA Department of Forest Rangelandand Fire SciencesCollege of Natural ResourcesUniversity of Idaho875 Perimeter DriveMoscowIdaho 83844-1133USA School of the Environment Washington State UniversityBox 646410PullmanWashington 99164-6420USA Wilderness Institute W.A.Franke College of Forestry and ConservationUniversity of Montana32 Campus DriveMissoulaMontana 59812USA

The reintroduction of fire to landscapes where it was once common is considered a priority to restore historical forest dynamics,including reducing tree density and decreasing levels of woody biomass on the forest ***,reintroducing fire causes tree mortality that can have unintended ecological outcomes related to woody biomass,with potential impacts to fuel accumulation,carbon sequestration,subsequent fire severity,and forest *** this study,we examine the interplay between fire and carbon dynamics by asking how reintroduced fire impacts fuel accumulation,carbon sequestration,and subsequent fire severity *** pre-fire,and continuing 6 years post-fire,we tracked all live,dead,and fallen trees≥1 cm in diameter and mapped all pieces of deadwood(downed woody debris)originating from tree boles≥10 cm diameter and≥1 m in length in 25.6 ha of an Abies concolor/Pinus lambertiana forest in the central Sierra Nevada,California,*** also tracked surface fuels along 2240 m of planar transects pre-fire,immediately post-fire,and 6 years *** years after moderate-severity fire,deadwood≥10 cm diameter was 73 Mg ha^(−1),comprised of 32 Mg ha^(−1) that persisted through fire and 41 Mg ha^(−1) of newly fallen wood(compared to 72 Mg ha^(−1) pre-fire).Woody surface fuel loading was spatially heterogeneous,with mass varying almost four orders of magnitude at the scale of 20 m×20 m quadrats(minimum,0.1 Mg ha^(−1);mean,73 Mg ha^(−1);maximum,497 Mg ha^(−1)).Wood from large-diameter trees(≥60 cm diameter)comprised 57%of surface fuel in 2019,but was 75%of snag biomass,indicating high contributions to current and future fuel *** of fire does not consume all large-diameter fuel and generates high levels of surface fuels≥10 cm diameter within 6 *** fires are needed to reduce surface fuel loading.

关键词： California Rim Fire Fuel reduction Sierra Nevada mixed-conifer Smithsonian ForestGEO Yosemite Forest Dynamics Plot Yosemite National Park