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Fundamentals and application in phytoremediation of an efficient arsenate reducing bacterium pseudomonas putida ARS1

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Journal of Environmental Sciences 2024年第3期137卷 237-244页

作者： Ze-Wen Wang Guang Yang Jian Chen Yaoyu Zhou Avelino Nunez Delgado Hui-Ling Cui Gui-Lan Duan barry p.rosen Yong-Guan Zhu Henan Institute of Advanced Technology Zhengzhou UniversityZhengzhou 450052China State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Department of Cellular Biology and pharmacology Florida International UniversityHerbert Wertheim College of MedicineMiamiFL33199USA University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China College of Resources and Environment Hunan Agricultural UniversityChangsha 410128China Department of Soil Science and Agricultura Chemistry Engineering Polytechnic SchoolUniversity of Santiago de CompostelaCampus Univ.s/n27002LugoSpain

Arsenic is a ubiquitous environmental ***-mediated arsenic biotransformations significantly infuence arsenic mobility and *** transformations by soil and aquatic organisms have been well documented,while little is known regarding effects due to endophytic *** endophyte pseudomonas putida ARS1 was isolated from rice grown in arsenic contaminated *** ARS1 shows high tolerance to arsenite(As(Ⅲ))and arsenate(As(V)),and exhibits efficient As(V)reduction and As(Ⅲ)effux *** exposed to 0.6 mg/L As(V),As(V)in the medium was completely converted to As(Ⅲ)by *** ARS1 within 4 *** sequencing showed that *** ARS1 has two chromosomal arsenic resistance gene clusters(arsRCBH)that contribute to efficient As(V)reduction and As(Ⅲ)effux,and result in high resistance to *** globosa is a strong arsenic accumulator with high potential for arsenic phytoremediation,which takes up As(Ⅲ)more efficiently than As(V).Co-culture of *** ARS1 and *** enhanced arsenic accumulation in *** by 69%,and resulted in 91%removal of arsenic(at initial concentration of 0.6 mg/L As(V))from water within 3 *** study provides a promising strategy for in situ arsenic phytoremediation through the cooperation of plant and endophytic bacterium.

关键词： Arsenic pseudomonas putida Arsenate reduction Endophyte Wolffia globosa phytoremediation

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丛毛单胞菌JL40中pIB-type ATpase AntA介导的锑抗性解毒机制

丛毛单胞菌JL40中PIB-type ATPase AntA介导的锑抗性解毒机制

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第十届重金属污染防治技术及风险评价研讨会

作者：安立进罗雄吴明晗奉礼玲史凯祥王革娇 barry p.rosen 李明顺华中农业大学生命科学技术学院农业微生物国家重点实验室

锑与砷一样,属于第五主族的毒性类金属,广泛分布于环境中。我们通过锑抗性细菌*** JL40的比较蛋白质组学发现了一个锑抗性操纵子(ant operon)。该操纵子包括三个基因:antR,antC和antA。AntR属于ArsR/SmtB金属调控蛋白家族,作为响应锑的...

锑与砷一样,属于第五主族的毒性类金属,广泛分布于环境中。我们通过锑抗性细菌*** JL40的比较蛋白质组学发现了一个锑抗性操纵子(ant operon)。该操纵子包括三个基因:antR,antC和antA。AntR属于ArsR/SmtB金属调控蛋白家族,作为响应锑的转录调控因子调控锑操纵子的表达。AntA属于一种新型的pIB-type ATpase,它在保守氨基酸结构以及转运底物上均不同于已报道的七种pIB-type ATpase,我们将其定义为pIB-8 ATpase。在*** AW3110中异源表达AntA赋予了菌株对Sb(III)的抗性,同时降低了细胞内Sb(III)的积累,而AntA对As(III)等其他金属没有外排作用,说明AntA具有Sb(III)的底物特异性。通过膜翻转实验进一步证明了AntA的Sb(III)特异性。AntC是一种具有潜在Sb(III)结合位点的小蛋白,AntC与AntA的共表达增加了对Sb(III)的抗性。我们推测AntC可作为AntA的Sb(III)伴侣蛋白,从而进一步促进Sb(III)的外排。新型Sb(III)外排pIB-type ATpase的鉴定增强了我们对环境中微生物的锑代谢的了解,为锑污染的生物治理奠定了理论基础。

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The European registry of quality outcomes for cataract and refractive surgery(EUREQUO):a database study of trends in volumes,surgical techniques and outcomes of refractive surgery

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Eye and Vision 2015年第1期2卷 76-84页

作者： Mats Lundström Sonia Manning peter barry Ulf Stenevi Ype Henry paul rosen Department of Clinical Sciences OphthalmologyFaculty of MedicineLund UniversityLundSweden Department of Ophthalmology Mater Misericordiae University HospitalDublinIreland Department of Ophthalmology St.Vincent’s University HospitalDublinIreland Department of Ophthalmology Sahlgren’s University HospitalMolndalSweden Department of Ophthalmology VumcAmsterdamThe Netherlands Oxford Eye Hospital OxfordUK.

Background:A European web-based registry for refractive surgery was established in 2008;The European Registry of Quality Outcomes for Cataract and Refractive Surgery(EUREQUO).The aim of the registry was to improve treatment and standards of care for refractive *** aims were to offer a tool for benchmarking by establishing a reference database and for surgeons to enter and analyze their own *** purpose of this study was to characterize the registry and analyze the data collected during its first ***:The characteristics of the web-based registry are *** collected from February 4^(th) 2004 until June 30th 2014 are included in the *** database is analyzed in terms of surgical technique,indications for surgery,complications,and refractive and visual ***:Data have been reported from 47 centers in 14 countries until *** 4,000 procedures were reported *** most frequent procedure was laser-assisted in-situ keratomileusis(LASIK)with 11697 reported *** time in the database,LASIK declined(pphotorefractive keratectomy(pRK)and refractive lens exchange(RLE)increased(pprocedures).The indications for surgery,in terms of preoperative refraction and age,were stable over time,for all types of *** complications were reported infrequently and with a well-known relationship to the type of surgical *** reported refractive outcomes were *** visual outcomes indicate a significant increase of visual acuity after high myopia treatment by phakic intraocular lens in the anterior(phakic IOL AC)and the posterior(phakic IOL pC)chamber and a poorer visual outcome,after both myopia and hyperopia treatment,by epithelial LASIK(Epi-LASIK).Conclusions:We describe the establishment of a European registry for refractive *** database increases at a rate of approximately 4000 refractive procedures per *** most frequent procedure is LASIK,but

关键词： Refractive surgery Registry European database Outcomes

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Functional oesophago-gastric junction imaging

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World Journal of Gastroenterology 2006年第18期12卷 2818-2824页

作者： barry p McMahon Asbjφrn M Drewes Hans Gregersen Department of Medical physics and Clinical Engineering Adelaide and Meath Hospital DublinIncorporating the National Children's HospitalTallaghtDublin 24Ireland Centre for Visceral Biomechanics & pain Aalborg HospitalAalborgDenmark

Despite its role in disease there is still no definitive method to assess oesophago-gastric junction competence （OGJ）. Traditionally the OGJ has been assessed using manometry with lower oesophageal sphincter pressure as the indicator. More recently this has been shown not to be a very reliable marker of sphincter function and competence against reflux. Disorders such as gastro-oesophageal reflux disease and to a lesser extend achalasia still effects a significant number of patients. This review looks at using a new technique known as impedance planimetry to profile the geometry and pressure in the OGJ during distension of a bag. The data gathered can be reconstructed into a dynamic representation of OGJ action. This has been shown to provide a useful representation of the OGJ and to show changes to the competence of the OGJ in terms of compliance and distensibility as a result of endoluminal therapy.

关键词： Oesophagogastric junction Lower oesophageal sphincter Competence Distensibility Functional imaging

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Do we really understand the role of the oesophagogastric junction in disease?

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World Journal of Gastroenterology 2009年第2期15卷 144-150页

作者： barry p McMahon Blair A Jobe John E pandolfino Hans Gregersen Department of Medical physics & Clinical Engineering Adelaide & Meath Hospital Tallaght Dublin 24 Ireland Associate professor of Surgery Director of Esophageal Diagnostics & Therapeutic Endoscopy Heart Lung and Esophageal Surgery Institute University of Pittsburgh Medical Centre Shadyside Medical Building Suite 715 Pittsburgh PA 15232 United States Division of Gastroenterology Northwestern Memorial University Hospital 676 N. St. Clair St. Suite 1400 Chicago IL 60611 United States professor of Biomechanics Director of Research Mech-Sense Aalborg Hospital Science and Innovation Centre Sφndre Skovvej 15 9000 Aalborg Denmark

The role of the oesophago-gastric junction (OGJ) in gastro-oesophageal reflux disease is still not completely understood, and there is no clinically used method to assess the OGJ function in patients. Only indirect methods such as pH studies are carried out. The OGJ acts a valve controlling the flow of solids, liquids and gases between the oesophagus and the stomach. Manometry can determine if a sphincter is toned or relaxed; but, it cannot confirm that the sphincter region is actually open. Distension is a new technique for measuring function in the OGJ. By measuring the cross-sectional area through the narrow region in the junction during distension of a catheter mounted bag, much more information on the opening and closing patterns of the junction can be determined. This technique has already been demonstrated to show changes in the OGJ after surgical treatments for reflux disease. New measurement ideas around the concept of distending the OGJ offer new hope that a clinically useable test for compliance at the junction can be developed and could potentially help in determining appropriate therapy.

关键词： Achalasia Distensibility Impedance planimetry Intraluminal impedance manometry Oesophago-gastric junction

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Computation of flow through the oesophagogastric junction

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World Journal of Gastroenterology 2007年第9期13卷 1360-1364页

作者： barry p McMahon Karl D Odie Kenneth W Moloney Hans Gregersen Department of Medical physics and Clinical Engineering Adelaide and Meath Hospital Dublin Incorporating the National Children’s Hospital Tallaght Dublin 24Ireland IMed Centre Department of Mechanical Engineering Institute of Technology Tallaght Dublin 24 Ireland Center for Visceral Biomechanics and pain Aalborg Hospital Aalborg DK-9100 DenmarkIMed Centre Institute of Technology Tallaght Dublin 24 Ireland

Whilst methods exist to indirectly measure the effects of increased flow or gastro-oesophageal refluxing, they cannot quantitatively measure the amount of acid travelling back up into the oesophagus during reflux, nor can they indicate the flow rate through the oesophagogastric junction (OGJ). Since OGJ dysfunction affects flow it seems most appropriate to describe the geometry of the OGJ and its effect on the flow. A device known as the functional lumen imaging probe (FLIp) has been shown to reliably measure the geometry of and pressure changes in the OGJ. FLIp cannot directly measure flow but the data gathered from the probe can be used to model flow through the junction by using computational flow dynamics (CFD). CFD uses a set of equations known as the Navier-Stokes equations to predict flow patterns and is a technique widely used in engineering. These equations are complex and require appropriate assumptions to provide simplifications before useful data can be obtained. With the assumption that the cross-sectional areas obtained via FLIp are circular, the radii of these circles can be obtained. A cubic interpolation scheme can then be applied to give a high-resolution geometry for the OGJ. In the case of modelling a reflux scenario, it can be seen that at the narrowest section a jet of fluid squirts into the oesophagus at a higher velocity than the fluid surrounding it. This jet has a maximum velocity of almost 2 ms-1 that occurs where the OGJ is at its narrowest. This simple prediction of acid ‘squirting’ into the oesophagusillustrates how the use of numerical methods can be used to develop a better understanding of the OGJ. This initial work using CFD shows some considerable promise for the future.

关键词： Computational flow dynamics model Oesophagus

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Non-Uniformity and Generalised Sacks Splitting

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Acta Mathematica Sinica,English Series 2002年第2期18卷 327-334页

作者： COOpER S.barry Department of pure Mathematics School of Mathematics University of Leeds Leeds UK Institute of Software Chines Academy of Sciences Beijing P. R. China

We show that there do not exist computable fimetions f_1(e,i).f_2(e,i).g_1(e,i),g_2(e,i)such that for all e,i ∈ω, (1)(W_(f_1)(e,i)-W_(f_2)(e,i))≤T(W_e-W_1): (2)(W_(g_1)(e,i)-W_(g_2)(e,i))≤T(W_e-W_i): (3)(W_w-W_i)≤T(W_(f_1)(e,i)-W_(f_2)(e,i))⊕(W_(g_1)(e,i)-W_(g_2)(e,i)): (4)(W_e-W_i)T(W_(f_1)(e,i)-W_(f_2)(e,i))uuless(W_e-W_i)≤T:and (5)(W_e-W_i)T(E_(g_1)(e,i)-W_(g_2)(e,i))unless(W_w-W_i)≤T. It follows that the splitting theorems of Sacks and Cooper cannot be combined uniformly.

关键词： Computably enumerable(c.e.) Difference of computably enumerable sets(d.c.e.,or 2-c.e.) Turing degrees Splitting and nonsplitting

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A History of Fischer-Tropsch Wax Upgrading at Bp-from Catalyst Screening Studies to Full Scale Demonstration in Alaska

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Journal of Natural Gas Chemistry 2006年第1期15卷 1-10页

作者： John p. Collins Joep J. H. M. Font Freide barry Nay Bp Exploration America Inc. Bp Exploration Operating Company

Conversion of Fischer-Tropsch wax into high quality synthetic crude or finished transportation fuels such as premium diesel has been studied over the past 15 years within Bp. Catalyst screening and selection was carried out in dedicated micro-reactors and pilot plants, whose designs are critical to the performance selection. Variation in catalyst composition and defining the gas to oil feed ratios with the operating temperature are a few of the parameters studied. product selection and maximizing diesel yield combined with stability （catalyst life） were the ultimate drivers. The selected catalyst was then tested under commercial conditions in a dedicated 300 barrel per day demonstration plant. The products were also tested in engines to assess their combustion characteristics.

关键词： Fischer-Tropsch wax upgrading hydrocracking synthetic crude diesel naphtha engine emission

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Impact of a single bout of high-intensity interval exercise and short-term interval training on interleukin-6, FNDC5, and METRNL mRNA expression in human skeletal muscle

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Journal of Sport and Health Science 2018年第2期7卷 191-196页

作者： Malcolm Eaton Cesare Granata Julianne barry Adeel Safdar David Bishop Jonathan p.Little School of Health and Exercise Science University of British Columbia Okanagan Institute of Sport Exercise and Active Living (ISEAL)College of Sport and Exercise ScienceVictoria University Department of pediatrics Mc Master University

Background: Exercise promotes numerous phenotypic adaptations in skeletal muscle that contribute to improved function and metabolic capacity. An emerging body of evidence suggests that skeletal muscle also releases a myriad of factors during exercise, termed "myokines". The purpose of this study was to examine the effects of high-intensity interval training(HIIT) on the acute regulation of the mRNA expression of several myokines, including the prototypical myokine interleukin-6(IL-6), and recently identified myokines fibronectin type III domain-containing protein 5(FNDC5)(irisin) and meteorin-like protein(METRNL).Methods: Both before and after a 20-day period of twice-daily high-volume HIIT, 9 healthy males(20.5 ± 1.5 years performed a standardized bout of high-intensity interval exercise(HIIE; 5 × 4 min at ~80% pretraining peak power output) with skeletal muscle biopsy samples(vastus lateralis) obtained at rest, immediately following exercise, and at 3 h ***: Before training, a single bout of HIIE increased IL-6(p p pression measured at 3 h recovery when compared to rest. Following 20 days of HIIT, IL-6 and FNDC5 mRNA were increased at 3 h recovery from the standardized HIIE bout when compared to rest(both p pression were higher following training(p post-training(main effect of training, p pregulation of skeletal muscle IL-6 mRNA both before and after a period of intensified HIIT;(2) Resting and overall FNDC5 mRNA expression is increased by 20 days of HIIT; and(3) METRNL mRNA expression is responsive to both acute HIIE and short-term intense HIIT. Future studies are needed to confirm these findings at the protein and secretion level in humans.

关键词： Brown adipose tissue Exerkines High-intensity interval training Intermittent exercise Myokine Obesity

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

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Frontiers of Chemical Science and Engineering 2018年第2期12卷 311-314页

作者： Adam C. Sedgwick Alex Hayden barry Hill Steven D. Bull Robert B. p. Elmes Tony D. James Department of Chemistry University of Bath Bath BA2 7AY UK Department of Chemistry Maynooth University Maynooth Co. Kildare Ireland

A simple nitrobenzyl-umbelliferone （NCOU1） was synthesised containing a nitroreductase （NTR） trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

关键词： fragmentation NCOU1 NTR nitroreductase nitrobenzyl-umbelliferone

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