检索结果-南通市图书馆

Effects of perinatal stress on the metabolites and lipids in plasma of dairy goats

在线全文

学校读者我要写书评

暂无评论

Stress Biology 2023年第1期3卷 126-144页

作者： Yan Huang Yezi Kong Bowen Li Chenxu zhao Juan J.Loor Panpan Tan Yang Yuan Fangyuan Zeng Xiaoyan Zhu Simeng Qi baoyu zhao Jianguo Wang College of Veterinary Medicine Northwest A&F UniversityYangling 712100ShaanxiChina LipidALL Technologies Company Limited Changzhou 213022JiangsuChina Department of Animal Sciences Division of Nutritional SciencesUniversity of IllinoisUrbanaIL 61801USA

Dairy goats experience metabolic stress during the peripartal period,and their ability to navigate this stage of lactation is related to the occurrence and development of metabolic *** dairy cows,there is a lack of comprehensive analysis of changes in the plasma profiles of peripartal dairy goats,particularly using high-throughput techniques.A subset of 9 clinically-healthy dairy goats were used from a cohort of 96 primiparous Guanzhong dairy goats(BCS,2.75±0.15).Blood samples were collected at seven time points around parturition(d 21,14,7 before parturition,the day of kidding,and d 7,14,21 postpartum),were analyzed using untargeted metabolomics and targeted *** orthogonal partial least squares discriminant analysis model revealed a total of 31 differential metabolites including p-cresol sulfate,pyruvic acid,cholic acid,and oxoglutaric *** pathway enrichment analysis identified phenylalanine metabolism,aminoacyl-tRNA biosynthesis,and citrate cycle as the top three significantly-altered *** Limma package identified a total of 123 differentially expressed ***(PS),free fatty acids(FFA),and acylcarnitines(ACs)were significantly increased on the day of kidding,while diacylglycerols(DAG)and triacylglycerols(TAG)***(Cer)and lyso-phosphatidylinositols(LPI)were significantly increased during postpartum period,while PS,FFA,and ACs decreased postpartum and gradually returned to antepartum *** species of FFA and phosphatidylcholines(PC)were segregated based on the differences in the saturation and length of the carbon ***,this work generated the largest repository of the plasma lipidome and metabolome in dairy goats across the peripartal period,which contributed to our understanding of the multifaceted adaptations of transition dairy goats.

关键词： Dairy goat Transition period Untargeted metabolomics Lipidomics

IL-13/IL-13RA2 signaling promotes colorectal cancer stem cell tumorigenesis by inducing ubiquitinated degradation of p53

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第1期11卷 495-508页

作者： baoyu He Jing Liang Qianqian Qin Yuqin Zhang Shuo Shi Jinghe Cao Zhixin Zhang Qingli Bie Rou zhao Li Wei Baogui Zhang Bin Zhang Department of Laboratory Medicine Affiliated Hospital of Jining Medical UniversityJining Medical UniversityJiningShandong 272000China Postdoctoral Mobile Station of Shandong University of Traditional Chinese Medicine JinanShandong 250355China Department of Reproductive Medicine Affiliated Hospital of Jining Medical UniversityJining Medical UniversityJiningShandong 272000China Department of Gastrointestinal Surgery Affiliated Hospital of Jining Medical UniversityJining Medical UniversityJiningShandong 272000China Institute of Forensic Medicine and Laboratory Medicine Jining Medical UniversityJiningShandong 272067China

Cancer stem cells(CSCs)are considered tumor-initiating cells and the main drivers of disease *** these rare cancer cells,however,remains challenging with respect to therapeutic ***,we report the up-regulation of IL-13RA2 expression in colorectal cancer(CRC)tissues and spheroid *** expression of IL-13RA2 was positively correlated with canonical stemness markers in *** further demonstrated that the level of IL-13 was up-regulated in the serum of CRC ***,recombinant IL-13(rIL13)stimulation promoted the sphere formation,proliferation,and migration of CRC cells in vitro and enhanced tumorigenesis in *** phenotype could be reversed by knocking down ***,IL-13 activated autophagy by inducing LC3I/LC3II transformation in CRC-CSCs,which was crucial for the biological functions of *** further demonstrated that IL-13RA2 acted as a modular link of the E3 ligase UBE3C and the substrate p53 protein,enhancing the interaction of UBE3C and p53,thereby inducing the K48-linked ubiquitination of *** conclusion,the IL-13/IL-13RA2 signaling cascade promotes CRC-CSC self-renewal and tumorigenesis by inducing p53 ubiquitination,adding an important layer to the connection between IL-13 and p53,which can be translated into novel targeted therapies.

关键词： Autophagy Colorectal cancer stem cells IL-13/IL-13RA2 signaling p53

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

电信网络诈骗共犯参与行为的刑事责任分析

法律适用 2023年第12期 105-115页

作者：赵宝玉中国政法大学刑事司法学院北京市第一中级人民法院

电信网络诈骗犯罪成功实施通常需要借助信息技术支持或转账取款两类帮助行为。应当回归传统共犯理论对此两类行为成立帮助犯与否进行分类、规范认定。对于信息技术支持类帮助犯刑事责任的认定应当侧重于主观面向,行为人需对其实施的帮... 详细信息

电信网络诈骗犯罪成功实施通常需要借助信息技术支持或转账取款两类帮助行为。应当回归传统共犯理论对此两类行为成立帮助犯与否进行分类、规范认定。对于信息技术支持类帮助犯刑事责任的认定应当侧重于主观面向,行为人需对其实施的帮助行为以及对正犯所实施的特定构成要件行为达至“双重故意”,并基于默示的意思联络、特别认知与正犯之间形成强烈的组织性联结。对于转账取款类帮助犯刑事责任的认定应当侧重于客观面向,结合电信网络诈骗犯罪特点,将资金转入作为诈骗既遂判定标准,排除诈骗罪既遂后实质性终了前承继帮助犯的认定。在符合传统共犯理论关于帮助犯主观、客观层面认定标准的基础上,电信网络诈骗犯罪的帮助行为满足“量身定制”“组织管理”“长期固定”三种类型特征之一,可认定为诈骗罪帮助犯。

关键词：电信网络诈骗犯罪诈骗罪帮助犯双重故意默示意思联络承继的帮助犯

Er^(3+)/Yb^(3+) Codoped Phosphate Glass for Ion-Exchanged Planar Waveguide Amplifiers

在线全文

学校读者我要写书评

暂无评论

光学学报 2003年第S1期23卷 98-99页

作者： Shilong zhao baoyu Chen Zhuping Liu Lili Hu Shanghai Institute of Optics and Fine Mechanics, The Chinese Academy of Sciences, P.R. China Shanghai Institute of Optics and Fine Mechanics The Chinese Academy of Sciences P.R. China

A novel Er3+/Yb3+ codoped phosphate glass was developed, which exhibited good chemical durability in molten salts and excellent spectroscopic properties. Preliminary results of ion exchange at different time and temperature, and with varying melt concentrations indicated that WM4 glass was suitable for ion-exchange experiments and there was no deterioration of surface quality.

关键词： Er3+ /Yb3+ codoped chemical durability ion exchange waveguide

间作及减量灌溉对农田土壤温室气体排放的互作效应

在线全文

学校读者我要写书评

暂无评论

干旱地区农业研究 2024年第1期42卷 214-225页

作者：殷民兴赵财葛丽丽程宝钰甘肃农业大学农学院省部共建干旱生境作物学国家重点实验室甘肃兰州730070

基于2014年在甘肃省武威市设置的不同灌水水平与种植模式定位试验,设置两种灌水水平,即减量灌水(495 mm, I1)和传统灌水(540 mm, I2);3种种植模式,即玉米‖豌豆(M‖P)、单作豌豆(P)、单作玉米(M),测定土壤呼吸速率和N_(2)O排放速率,并... 详细信息

基于2014年在甘肃省武威市设置的不同灌水水平与种植模式定位试验,设置两种灌水水平,即减量灌水(495 mm, I1)和传统灌水(540 mm, I2);3种种植模式,即玉米‖豌豆(M‖P)、单作豌豆(P)、单作玉米(M),测定土壤呼吸速率和N_(2)O排放速率,并计算农田温室气体排放总量、全球增温潜势(GWP)和温室气体排放强度(GHGI)。2021—2022两年结果表明:(1)I1和M‖P处理均可显著降低土壤呼吸速率、N_(2)O排放速率、CO_(2)排放总量和N_(2)O排放总量(P M‖P> P;相关性分析表明,土壤温度和土壤含水量与土壤呼吸速率和N_(2)O排放速率呈显著正相关关系。(3)灌水水平对作物产量无显著影响(P>0.05),种植模式对作物产量影响显著(P<0.01),表现为M‖P处理产量最高;减量灌水与种植模式交互效应对GWP和GHGI影响显著(P<0.05),MPI1处理较MPI2处理的GWP和GHGI分别降低9.45%和10.00%。在绿洲灌区,减量灌水结合玉米‖豌豆种植模式能够有效提高作物产量并减少温室气体排放,可作为河西灌区推广应用的种植模式。

关键词：间作减量灌水土壤温室气体排放玉米豌豆

在线全文

学校读者我要写书评

暂无评论

淀粉湿热处理改性效果强化研究进展

中国粮油学报 2024年第4期39卷 189-201页

作者：柴宝玉叶发银雷琳陈嘉赵国华西南大学食品科学学院重庆400715 川渝共建特色食品重庆市重点实验室重庆400715

湿热处理是一种物理改性方式,因操作简便、安全高效,目前被广泛应用在淀粉的改性当中,但传统湿热处理存在处理时间长、处理效果不足等问题。因此,采取一定方式强化淀粉湿热处理的改性效率和效果具有重要的实际意义。本文系统梳理总结了... 详细信息

湿热处理是一种物理改性方式,因操作简便、安全高效,目前被广泛应用在淀粉的改性当中,但传统湿热处理存在处理时间长、处理效果不足等问题。因此,采取一定方式强化淀粉湿热处理的改性效率和效果具有重要的实际意义。本文系统梳理总结了反复湿热处理、物理强化、化学强化、酶强化以及其他强化对淀粉多尺度结构和理化特性的影响及具体强化机理,为研究开发有效的淀粉湿热处理的强化方法提供一定的参考。

关键词：淀粉强化湿热效果加工特性机理

同方期刊数据库

Crystal structure of a cell surface receptor（uPAR） in its ligand-free conformation reveals the exposure of an unusually large hydrophobic area

在线全文

学校读者我要写书评

暂无评论

Crystal structure of a cell surface receptor（uPAR） in its ...

第四届中国结构生物学学术讨论会

作者： baoyu zhao Cai Yuan Rui Li Michael Ploug Nicolai Sidenius Mingdong Huang Fujian Institute of Research on the Structure of Matter The Chinese Academy of Sciences Finsen Laboratory Rigshospitalet & Biotech Research and Innovation Centre (BRIC) Copenhagen Biocenter Ole Maal?es Vej 5 DK-2200 Copenhagen N Denmark San Raffaele Scientific Institute

Proteins typically fold in a way to have their hydrophobic residues buried in the core of proteins while the hydrophilic residues exposed on protein surface. The exposure of large hydrophobic patch to solvent is thermodynamically unfavorable, and is not commonly observed. Cell surface receptor urokinase-type plasminogen activator receptor（uPAR） has a large hydrophobic patch in its central ligand binding pocket. Upon the ligand（uPA） binding, this hydrophobic patch on uPAR is sequestered, forming a highly stable and tight-binding complex（Qing Huai, 2006）. The crystallization of uPAR ectodomains in its ligand-free conformation is expected to be difficult, but has been a long sought goal. With the help of an anti-uPAR antibody（8B12）, we recently succeed to crystallize the uPAR:8B12 complex in the absence of any ligands, and determined the crystal structure of uPAR in the ligand-free conformation. Surprisingly, uPAR adopts a conformation similar to its ligand-bound state, exposing a large hydrophobic area to solvent. This observation is different from the previous speculation and biochemical studies（Cai Yuan, 2007 and Haydyn D. T. Mertens, 2012）. uPAR is a glycolipid-anchored cell surface receptor and consists of three Ly6/uPAR/α-neurotoxin-like（LU） domains. Extensive degree of inter-domain flexibility was expected and was indeed observed in solution by biochemical studies including small angle X-ray scattering. However, Our recent uPAR:8B12 structure showed no inter-domain flexibility and the conformation of unliganded uPAR is identical to the ligand-bound conformation. Further functional studies are underway to examine the functional consequences of these structural observations.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

苦马豆素历史、来源及生物活性研究进展

中国兽医学报 2022年第11期42卷 2326-2336页

作者：黄睿杰马永嘉赵世姣王帅吴晨晨路浩赵宝玉西北农林科技大学动物医学院陕西杨凌712100 阿拉善左旗动物疫病预防控制中心内蒙古阿拉善750300 昌都市畜牧总站西藏昌都854000

苦马豆素是一种含羟基吲哚里西啶生物碱,从发现命名至今已经有40多年的历史,起初被确证是疯草类有毒植物的毒性成分,研究重点主要是侧重其毒性与毒理方面,证实苦马豆素可特异性的抑制动物机体α-甘露糖甘酶活性,导致细胞溶酶体甘露聚糖... 详细信息

苦马豆素是一种含羟基吲哚里西啶生物碱,从发现命名至今已经有40多年的历史,起初被确证是疯草类有毒植物的毒性成分,研究重点主要是侧重其毒性与毒理方面,证实苦马豆素可特异性的抑制动物机体α-甘露糖甘酶活性,导致细胞溶酶体甘露聚糖蓄积,造成细胞空泡变性,引起器官组织损伤和神经功能障碍。随着研究的深入,人们又发现苦马豆素具有抗肿瘤、抗病毒和免疫调节等生物活性,从此掀起了苦马豆素药理作用研究的热潮,引起人类医学研究领域学者的关注和极大兴趣。现根据国内外苦马豆素相关研究文献,从其研究历史、主要来源、毒性与毒理及药理活性等方面进行归纳总结,并对今后的研究方向进行展望,以期为人们较全面地掌握苦马豆素的历史、最新研究进展、以及科学认识苦马豆素的两重性提供重要理论参考。

关键词：苦马豆素历史来源毒性药理活性疯草

The Mechanism of Swainsonine Causing Early Pregnancy Abnormal Decidualization and Inducing Abortion by Changing Glycosylation Modification

在线全文

学校读者我要写书评

暂无评论

Advances in Reproductive Sciences 2018年第3期6卷 70-101页

作者： Yi Wang Xin Gao Ming Peng Yanfei You Fangyun Shi Yazhou Guo baoyu zhao Chenchen Wu Northwest A & F University Animal Medical College Yangling China

Locoweed is a poisonous plant that severely harms the development of grass-land and animal husbandry throughout the world. Oxytropis and Astragalus are the Latin names for the poisonous plants commonly known as locoweed. The main toxic substance in these plants is swainsonine. It is a strong inhibitor of lysosomal α-mannosidase I and Golgi body α-mannosidase II, resulting in the accumulation of oligosaccharides and glycoproteins in lysosomes, interfering with the molecular and cellular recognition of glycans, and causing tissue-cell vacuolar degeneration. Livestock shows a series of clinical and pathological symptoms, mainly for the decline of reproductive performance. The effects in dams are abortion, weak fetus, and fetal malformations. The key step in maintaining pregnancy in dams is early endometrial decidualization, because reproductive hormones, decidual cell apoptosis, endoplasmic reticulum (ER) stress, and other immune factors are negatively affected by swainsonine. This article addresses the modifications that occur through glycan processing and glycosylation resulting in the change of post-translational modification of the protein and the activity of the glycoprotein, and then direct and indirect mechanisms of ER stress, apoptosis, reproductive hormones, immune factors, and cell cycle and other pathways. Our aim is to find new methods of prevention and treatment of swainsonine poisoning in grassland animals.

关键词： Swainsonine Glycosylation Modification Abortion Decidua Hormone Cell Apoptosis Endoplasmic Reticulum Stress