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The Chinese-German Journal of Clinical Oncology 2010年第2期9卷 93-96页

作者： baoshan wang jihua zhang dongmei song ying zhang Department of Otolaryngology-Head and Neck Surgery The First Hospital Hebei Medical University Shijiazhuang 050031 China

Objective： The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by ^60Co γ-ray. Methods： Human laryngeal cancer Hep-2 cells were divided into 3 groups： group A （normoxia）, group B （hypoxia）, and group C （reoxygenation after hypoxia）. All of the cells were exposed to 5 Gy dosage of γ-ray. Flow cytometry （FCM） was used tomeasure the protein levels of HIF-1α and p53 and to detect cell apoptosis. The protein levels of HIF-la and p53 were also determined by immunohistochemistry and Western blotting. The expression of HIF-la mRNA was determined by RT-PCR. Results： The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A. The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in group C was higher than that in group B. Conclusion： Hypoxia decreased the effect of apoptosis induced by ^60Co γ-ray in Hep-2 human laryngeal cancer cell line. The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.

关键词： γ-ray hypoxia apoptosis p53 Hep-2 cell

The impact of ^(60)Co γ-ray on cycles to Hep-2 human laryngeal cancer cell in the condition of hypoxia

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The Chinese-German Journal of Clinical Oncology 2010年第7期9卷 373-377页

作者： baoshan wang Guibo Li dongmei song jihua zhang Department of Otolaryngology-Head and Neck Surgery The First Hospital Hebei Medical University Shijiazhuang 050031 China Family Planning Service Station of Haigang area Qjnhuangdao 066000 China

Objective： The aim of the study was to investigate the impact of 60Co y-ray on apoptosis, cell cycles and the expression of protein hypoxia-inducible factor-1α （HIF-1α） to Hep-2 cell line in the conditions of normoxia and hypoxia. Methods： Hep-2 cell were divided into 2 groups： group A （normoxia） and group B （hypoxia）. All of the ceils were exposed to y-ray with dosage being 0, 1, 3, 5, 10, 20, and 40 Gy. Flow cytometry was used to measure the protein level of HIF-1α and to detect apoptosis and cell cycles. The protein level of HIF-1α was also determined by immunohistochemistry and Western blotting. Results： The protein level of HIF-1α in group B was significantly higher than that in group A. In group A, low doses （1-5 Gy） of y-ray had caused G0/G1 cell cycle arrest and high doses （10-40 Gy） had caused G2/M cell cycle arrest. In group B, without exposure of y-ray （0 Gy） had caused G0/G1 cell cycle arrest, all of the different dosage of y-ray could cause G2/M cell cycle arrest. The curve of apoptosis rate in group A was a parabola, the apoptotic rate was related to the dosage of y-ray in a dosage dependent manner. The peak was at the point of 5 Gy. The apoptosis rate in group A was significantly higher than that in group B. Conclusion： Different doses of y-ray could cause different cell cycles arrest then make different impact on apoptosis to Hep-2 ceil. The lower apoptosis rate in condition of hypoxia maybe has a relationship with G2/M cell cycle arrest. Up-regulated HIF-1α protein may be one of the reasons for G2/M cell cycle arrest.

关键词： cell cycle y-ray apoptosis hypoxia-inducible factor-1α (HIF-1α) Hep-2 cell

The impact on secreted VEGF of Hep-2 human laryngeal cancer cell induced by Rg3

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The Chinese-German Journal of Clinical Oncology 2013年第10期12卷 477-480页

作者： jihua zhang Lai wang Caili Han dongmei song baoshan wang Suqin Shi Sha Liu Department of Otolaryngology-Head and Neck Surgery The First Hospital of Hebei Medical University Intensive Care Unit the First Hospital of Hebei Medical University Hebei Medical University

Objective：The aim of this study was to investigate the af ecting of Rg3 to secreted VEGF of human laryngeal carcinoma Hep-2 cells and its mechanism of inhibition to tumor angiogenesis. Methods：Cultured human laryngeal cancer cellline Hep-2 and human vascular endothelial cells in vitro, cells got into the period of exponential phase of growth, was diviced into 3 groups：group I （control group）, group II （DDP group）, group III （Rg3 group）. Added to the Hep-2 cells Rg3 and DDP, made Rg3 final concentration was 300μg/mL, and DDP was 3μg/mL. 48 h later, specimens from sample to be done immunocytochemistry, and the protein of VEGF in Hep-2 cells to be detected. Col ecting Hep-2 cells supernatant, some was used to measure the protein level of VEGF in Hep-2 cells supernatant by ELISA. Some was used to culture HVEC. 24 h later, cellgrowth inhibition rate of human vascular endothelial was determined by MTT. Results：The protein level of VEGF was evi-dently higher in group I compared to group II and group III, it was not only in Hep-2 cells, but also in supernatant of Hep-2 cells. There was no significantly dif erent between group II and group III. MTT results showed that, the human vascular endothelial cellgrowth inhibition rate of group I was significantly lower than that of group II and group III （P〈0.05）. At the same time the HVEC growth inhibition rate of group II was significantly lower than that of group III （P〈0.05）. Conclusion：The inhibition to tumor angiogenesis of Rg3 is stronger than traditional chemotherapy drug cisplatin. It worke by reducing the biological ef ects of secreted VEGF, But the ef ecting worke by reducing the activity of secreted VEGF itself or af ecting endothelial function of VEGF receptor or some other ways to be further studied.

关键词： Rg3 secreted VEGF Hep-2 cell HVEC

阻塞性睡眠呼吸暂停低通气综合征患者颞横回氢质子波谱研究

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听力学及言语疾病杂志 2014年第3期22卷 264-267页

作者：宋冬梅张继华刘涛吕欣薛静王宝山河北医科大学第一医院耳鼻咽喉科石家庄050031

目的探讨磁共振氢质子波谱(1 H—MRS)用于阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者听皮层神经细胞损伤的预警作用。方法随机抽取经PSG监测确诊的OSAHS患者95例,根据纯音听阈将其分为OSAHS并双侧耳聋组(18例)、OSAHS并单侧耳聋组(27例... 详细信息

目的探讨磁共振氢质子波谱(1 H—MRS)用于阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者听皮层神经细胞损伤的预警作用。方法随机抽取经PSG监测确诊的OSAHS患者95例,根据纯音听阈将其分为OSAHS并双侧耳聋组(18例)、OSAHS并单侧耳聋组(27例,左15例,右12例)、单纯OSAHS组(50例),选择健康志愿者15例为正常对照组,各组分别行双侧颞横回听皮层1 H—MRS检测,根据神经代谢产物其峰下面积计算N-乙酰天门冬氨酸/肌酸(NAA/Cr)、胆碱/肌酸(Cho/Cr)、肌醇/肌酸(mI/Cr)、N-乙酰天门冬氨酸/胆碱(NAA/Cho);用ROC曲线面积>50%为判断上述各组指标诊断早期OSAHS并感音神经性听力损失的灵敏度及特异度。结果与正常对照组相比,OSAHS组以及OSAHS并耳聋组NAA/Cho降低(P0.05);NAA/Cho的ROC曲线下面积为73%,其余指标ROC曲线下的面积均<50%。结论 NAA/Cho有可能作为判定OASHS患者合并感音神经性听力损失的早期听皮层代谢预警指标。

关键词：呼吸睡眠暂停低通气综合症感音神经性聋磁共振氢质子波谱 Obstructive sleep apnea hypopnea syndrome (OSAHS ) 1H magnetic resonance spectroscopy(1H -MRS)

HIF-1α与LOX、PINCH蛋白在人喉鳞状细胞癌中的表达

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中国耳鼻咽喉头颈外科 2012年第11期19卷 588-592页

作者：董凯峰宋冬梅吕欣王宝山刘志明张继华河北医科大学第一医院耳鼻咽喉头颈外科河北石家庄050031

目的检测缺氧诱导因子-1α(Hypoxia-inducible transcription factor-1α,HIF-1α)、赖氨酰氧化酶(lysyloxidase,LOX)和PINCH(particularyinteresting new cysteine-histidine rich protein)蛋白在人喉鳞状细胞癌(简称鳞癌)中的表达,探讨三者在喉鳞癌发生发展、转移及预后的意义。方法采用免疫组织化学方法检测38例喉鳞癌组织中HIF-1α、LOX及PINCH的表达情况,并分析其表达的相关性。结果①HIF-1α、LOX和PINCH在喉鳞癌组织中表达显著高于癌旁黏膜组织;②HIF-1α和PINCH在淋巴结转移组与无转移组表达阳性率分别为100.0%、100.0%和67.7%、70.8%,统计学差异具有显著性意义;LOX在淋巴结转移组与无转移组表达,无显著性差异;③HIF-1α、LOX、PINCH的表达与患者的年龄、性别、原发部位、T分级及临床分期等临床病理因素无关;④HIF-1α在喉鳞癌中的表达与PINCH、LOX的表达存在相关性。结论 LOX、PINCH在人喉鳞癌中的表达存在相关性,可作为喉癌治疗的一个新靶点。HIF-1α的高表达可能是造成LOX、PINCH表达变化的重要影响因素之一。

关键词：癌鳞状细胞缺氧诱导因子1 免疫组织化学预后赖氨酰氧化酶 PINCH

不同人类干扰背景下横断山南段保护优先区规划研究

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生态学报 2021年第24期41卷 9655-9668页

作者：宋成文王文礼刘冬梅代光辉刘维鸿李泞吕朱颖张志明中国环境科学研究院北京100012 云南大学生态与环境学院昆明650223 云南省生态环境科学研究院昆明650034

建立自然保护区是保护和维持生物多样性最有效、最基本的措施。在有限的资金和人力条件下,如何参考不同人类干扰在保护优先区建设中的影响并选择合适的保护规划方案,在更大程度上的保护本区域的生物多样性,一直以来都是保护生物学家争... 详细信息

建立自然保护区是保护和维持生物多样性最有效、最基本的措施。在有限的资金和人力条件下,如何参考不同人类干扰在保护优先区建设中的影响并选择合适的保护规划方案,在更大程度上的保护本区域的生物多样性,一直以来都是保护生物学家争论的焦点。以横断山南段区为例,重点关注保护区建设过程中不同的人类干扰程度,以人类干扰的高低为切入点,基于多准则决策分析的原理和方法,以横断山南植被生态系统和人类干扰强度因子为基础,对比分析横断山南的生态系统保护价值分布、人类干扰格局和保护成效,结果显示:一是区域内森林生态系统(针阔混交林、常绿阔叶林、落叶阔叶林、落叶混交林、常绿针叶林)、湿地生态系统(湖泊、河流、草本湿地)、高山生态系统(冰川和永久积雪、高寒草甸、高寒草原)价值高且分值在21分以上;二是从人类干扰高低分析入手,基于维持和提升区域生物多样性的角度出发,提出了不同的保护优先区域和保护策略。三是识别出横断山南段区的保护优先区主要包括峨边县、马边县、石棉县、越西县、保山市、腾冲市、维西县、德钦县、察隅县等区域。

关键词：横断山南段区生态系统人类干扰保护优先区