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Chinese Journal of Chemical Engineering 2023年第12期64卷 1-8页

作者： Xiangyang Cui Xin Zhang baoju wang Yuqi Sun Haikui Zou Guangwen Chu Yong Luo Jianfeng Chen State Key Laboratory of Organic-Inorganic Composites Research Center of the Ministry of Education for High Gravity Engineering and TechnologyBeijing University of Chemical TechnologyBeijing 100029China School of Chemistry and Chemical Engineering Shandong University of TechnologyZibo 255000China

The catalytic hydrogenation of 2-nitro-4-acetylamino anisole(NMA)is a less-polluting and efficient method to produce 2-amino-4-acetamino anisole(AMA).However,the kinetics of catalytic hydrogenation of NMA to AMA remains obscure.In this work,the kinetic models including power-law model and Langmuir-Hinshelwood-Hougen-Watson(LHHW)model of NMA hydrogenation to AMA catalyzed by Raney nickel catalyst were investigated.All experiments were carried out under the elimination of mass transfer resistance within the temperature range of 70–100°C and the hydrogen pressure of 0.8–1.5 MPa.The reaction was found to follow 0.52-order kinetics with respect to the NMA concentration and 1.10-order kinetics in terms of hydrogen pressure.Based on the LHHW model,the dual-site dissociation adsorption of hydrogen was analyzed to be the rate determining step.The research of intrinsic kinetics of NMA to AMA provides the guidance for the reactor design and inspires the catalyst modification.

关键词： Intrinsic kinetics 2-nitro-4-acetylamino anisole Catalytic hydrogenation Raney nickel catalyst

SARS-CoV-2-specific T cell responses wane profoundly in convalescent individuals 10 months after primary infection

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Virologica Sinica 2023年第4期38卷 606-619页

作者： Ziwei Li Tiandan Xiang Boyun Liang Jing Liu Hui Deng Xuecheng Yang Hua wang Xuemei Feng Gennadiy Zelinskyy Mirko Trilling Kathrin Sutter Mengji Lu Ulf Dittmer baoju wang Dongliang Yang Xin Zheng Jia Liu Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Institute for Virology University Hospital of EssenUniversity of Duisburg-EssenEssen45147Germany Joint International Laboratory of Infection and Immunity Huazhong University of Science and TechnologyWuhan430022China

A key question in the coronavirus disease 2019(COVID-19)pandemic is the duration of specific T cell responses against the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)post primary infection,which is difficult to address due to the large-scale COVID-19 vaccination and re-exposure to the virus.Here,we conducted an analysis of the long-term SARS-CoV-2-specific T cell responses in a unique cohort of convalescent individuals(CIs)that were among the first to be infected worldwide and without any possible antigen re-exposure since then.The magnitude and breadth of SARS-CoV-2-specific T cell responses correlated inversely with the time that had elapsed from disease onset and the age of those CIs.The mean magnitude of SARS-CoV-2-specific CD4 and CD8 T cell responses decreased about 82%and 76%,respectively,over the time period of ten months after infection.Accordingly,the longitudinal analysis also demonstrated that SARS-CoV-2-specific T cell responses waned significantly in 75%of CIs during the follow-up.Collectively,we provide a comprehensive characterization of the long-term memory T cell response in CIs,suggesting that robust SARS-CoV-2-specific T cell immunity post primary infection may be less durable than previously expected.

关键词： COVID-19 SARS-CoV-2 T cell response Convalescence

A retrospective cohort study of methylprednisolone therapy in severe patients with COVID-19 pneumonia

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1979-1981页

作者： Yin wang Weiwei Jiang Qi He Cheng wang baoju wang Pan Zhou Nianguo Dong Qiaoxia Tong Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Rheumatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China Department of Hand Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 43000China

Dear Editor,Corona Virus Disease 2019(COVID-19)was first reported in late December 2019,in Wuhan,China.There are over 1,800,000 confirmed cases worldwide.1 The pathological process of severe COVID-19 pneumonia is an inflammation reaction characterized by the destruction of the deep airway and alveolar.2 It is currently considered that lung injury is not only associated with the direct virus-induced damage,but also the immune responses triggered by COVID-19 that lead to the activation of immune cells to release a large number of pro-and anti-inflammatory cytokines.Histologic examination has shown diffuse alveolar damage and mucinous exudate,which is similar to acute respiratory distress syndrome.2 Aggravation of symptoms always occurs during 5–7 days after onset in patients with COVID-19 pneumonia and severe cases develop rapidly to acute respiratory failure.3 Therefore,it is important to strengthen the treatment to suppress the proinflammatory response and control the cytokine storm at this stage.Methylprednisolone are the classical immunosuppressive drugs,which are important to stop or delay the progress of the pneumonia,and have been proved to be effective for the treatment of acute respiratory distress syndrome(ARDS).

关键词： pneumonia damage drugs

Safety and immunogenicity of a SARS-CoV-2 inactivated vaccine in patients with chronic hepatitis B virus infection

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Cellular & Molecular Immunology 2021年第12期18卷 2679-2681页

作者： Tiandan Xiang Boyun Liang Hua wang Xufeng Quan Shengsong He Helong Zhou Yongwen He Dongliang Yang baoju wang Xin Zheng Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Joint International Laboratory of Infection and Immunity Huazhong University of Science and TechnologyWuhanChina

Coronavirus disease 2019(COVID-19),caused by SARS-CoV-2 infection,has become a major global public health threat.Although significant advances have been made in developing and applying different vaccines in clinical trials[1,2],data are limited on the safety and efficacy of the inactivated vaccine in patients with chronic liver disease[3].Recent studies have preliminarily described the safety and immunogenicity of SARS-CoV-2 vaccines in patients with nonalcoholic fatty liver disease and in liver transplant recipients[4,5].

关键词： patients infection liver

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外源褪黑素对水培韭菜生长和品质的影响

园艺学报 2024年第4期51卷 847-858页

作者：郝金倩王宝驹佟静刘明池武占会王素娜刘宁河北工程大学园林与生态工程学院河北邯郸056038 北京市农林科学院蔬菜研究所国家蔬菜工程技术研究中心农业农村部华北都市农业重点实验室北京100097 北京市农林科学院蔬菜研究所蔬菜生物育种全国重点实验室北京100097

水培韭菜辛辣风味不足是限制产业发展难题之一,其主要原因是韭菜风味前体物质S–烃基半胱氨酸亚砜[S-alk(en)yl cysteine sulfoxide,CSO]积累不足。为提升水培韭菜风味,以‘791’韭菜为试材,研究含有不同浓度(25、50、75、100、150μmol... 详细信息

水培韭菜辛辣风味不足是限制产业发展难题之一,其主要原因是韭菜风味前体物质S–烃基半胱氨酸亚砜[S-alk(en)yl cysteine sulfoxide,CSO]积累不足。为提升水培韭菜风味,以‘791’韭菜为试材,研究含有不同浓度(25、50、75、100、150μmol·L^(-1))褪黑素的水培营养液对韭菜生长发育和品质的影响。结果表明:水培韭菜的株高、叶宽、叶长、根系活力、单株干(鲜)质量、理论产量等指标随外源褪黑素浓度的增加呈现先上升后下降的趋势,在褪黑素浓度为100μmol·L^(-1)时达到峰值;褪黑素处理显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)活性,显著降低丙二醛(MDA)和过氧化氢(H_(2)O_(2))含量;75或100μmol·L^(-1)褪黑素处理能有效促进韭菜可溶性糖、可溶性蛋白、维生素C、总黄酮、总酚等营养物质的积累,硝酸盐含量略有增加;褪黑素处理能显著提升韭叶辛辣度,编码CSO合成关键酶基因AtuFMO4/5/6的表达量也显著增加,它们可能与韭叶辛辣风味提高密切关联。综上,外源褪黑素处理有助于提升水培韭菜的产量和品质,75μmol·L^(-1)褪黑素处理能够兼顾韭菜产量、品质和生产成本,实现优质水培韭菜生产。

关键词：水培韭菜褪黑素 S–烃基半胱氨酸亚砜品质风味

基于迁移光子雪崩效应的单束低功率激光三维多色超分辨显微成像技术

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Science Bulletin 2024年第4期69卷 458-465页

作者：朱志旻梁宇森赵琪吴蕙潘彬雄乔书倩王保举詹求强 Centre for Optical and Electromagnetic Research Guangdong Engineering Research Centre of Optoelectronic Intelligent Information PerceptionSouth China Academy of Advanced OptoelectronicsSouth China Normal UniversityGuangzhou 510006China

The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modalities require either complicated/costly purpose-built systems such as multiple-beam architectures or complex post-processing procedures with intrinsic artifacts.Achieving three-dimensional(3D)or multi-channel sub-diffraction microscopic imaging using a simple method remains a challenging and struggling task.Herein,we proposed 3D highly-nonlinear super-resolution microscopy using a singlebeam excitation strategy,and the microscopy principle was modelled and studied based on the ultrahigh nonlinearity enabled by photon avalanches.According to the simulation,the point spread function of highly nonlinear microscopy is switchable among different modes and can shrink three-dimensionally to sub-diffraction scale at the photon avalanche mode.Experimentally,we demonstrated 3D optical nanoscopy assisted with huge optical nonlinearities in a simple laser scanning configuration,achieving a lateral resolution down to 58 nm(λ/14)and an axial resolution down to 185 nm(λ/5)with one single beam of low-power,continuous-wave,near-infrared laser.We further extended the photon avalanche effect to many other emitters to develop multi-color photon avalanching nanoprobes based on migrating photon avalanche mechanism,which enables us to implement single-beam dual-color sub-diffraction super-resolution microscopic imaging.

关键词： Super-resolution microscopy Nonlinear optics Upconversion nanoparticles Photon avalanches

氮硫互作对韭菜总辛辣程度及其同化关键酶的影响

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中国农业大学学报 2024年第8期29卷 95-108页

作者：张烨达佟静王宝驹刘宁季延海梁浩王丽萍武占会北京市农林科学院蔬菜研究所/农业农村部华北都市农业重点实验室北京100097 河北工程大学园林与生态工程学院河北邯郸056038 北京市农林科学院国有资产与基地管理处北京100097

为明确氮硫互作对韭菜植株总辛辣程度和氮、硫素同化关键酶活性的影响,以韭菜品种‘京韭一号’为试材,采用水培模式处理,试验设定3个氮浓度(6、12和18 mmol/L),3个硫浓度(2、4和8 mmol/L),随机区组试验,在植株生长至第7、14、21和28天这... 详细信息

为明确氮硫互作对韭菜植株总辛辣程度和氮、硫素同化关键酶活性的影响,以韭菜品种‘京韭一号’为试材,采用水培模式处理,试验设定3个氮浓度(6、12和18 mmol/L),3个硫浓度(2、4和8 mmol/L),随机区组试验,在植株生长至第7、14、21和28天这4个不同生育周期节点时分别测定韭菜叶片的酶促丙酮酸(EPY)含量及硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、ATP-硫酸化酶(ATPS)和氧乙酰丝氨酸(硫醇)裂解酶(OAS-TL)活性的动态变化。结果表明:EPY含量在植株生长至7、14与21 d时明显低于28 d;不同生育周期节点NR活性差异显著,氮硫素交互作用明显;GS活性响应程度剧烈并以植株长至28 d时水平最低;ATPS活性在植株长至7 d时水平最低且在中后期响应并不十分显著;OAS-TL活性呈现出平稳上升的趋势,但对氮硫素水平的敏感程度不及其他同化酶。韭菜发育过程中,氮素、硫素单一效应总体远低于元素间交互作用,氮硫素交互作用占据主导地位。韭菜植株硫素同化能力的提升与氮素水平密切相关,氮、硫素参与彼此的同化过程。综合考虑,建议水培韭菜营养液的氮素浓度为12 mmol/L,硫素浓度为2~4 mmol/L。该结论为水培韭菜氮、硫元素合理配施提供理论依据。

关键词：韭菜氮硫辛辣程度酶活性互作效应

中国旱獭Ⅰ型干扰素受体β亚基克隆、表达及功能初步鉴定

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临床肝胆病杂志 2024年第2期40卷 278-283页

作者：陶颖杨东亮王宝菊刘艺桂文甲李智樊和斌中国人民解放军中部战区总医院感染科武汉430030 华中科技大学同济医学院附属协和医院感染科武汉430030

目的克隆中国旱獭Ⅰ型干扰素受体β亚基(mhIFNAR2)的基因,并进行抗体制备及功能鉴定。方法应用RT-PCR技术,从中国旱獭脾组织中扩增得到序列,克隆至原核表达载体p RSET-B,表达重组蛋白,电泳和Western Blot法鉴定;重组蛋白常规免疫BALB/c... 详细信息

目的克隆中国旱獭Ⅰ型干扰素受体β亚基(mhIFNAR2)的基因,并进行抗体制备及功能鉴定。方法应用RT-PCR技术,从中国旱獭脾组织中扩增得到序列,克隆至原核表达载体p RSET-B,表达重组蛋白,电泳和Western Blot法鉴定;重组蛋白常规免疫BALB/c小鼠制备其胞外段多克隆抗体,免疫组化、免疫荧光和Western Blot法鉴定;再通过si RNA阻断的方法检测其功能。计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验。结果从mhIFNAR2扩增出149~1300 bp片段,其同源性在分析的种属中以土拨鼠最高,可达98.05%。成功地构建了表达胞外段mhIFNAR2_((50-181aa))蛋白的原核表达质粒,命名为pRSET-B.mhIFNAR2;其表达重组蛋白分子量27 kD,纯化后纯度约为95%,浓度约为160μg/mL。用纯化的重组蛋白常规免疫BALB/c小鼠后,获得1∶1000的特异性多克隆抗体,用免疫组化及免疫荧光可见细胞膜、细胞质有表达。合成的三条siRNA,其中有一条起始于277位点的siRNA(siRNA277)与空白对照及阴性对照相比,可以沉默目的基因的表达,并能减弱干扰素的信号通路(P值均<0.05)。结论获得mhIFNAR2的部分序列,成功地制备出抗mhIFNAR2胞外段多克隆抗体,该抗体有较高的效价和特异性,并能用于免疫组化、免疫荧光及Western Blot的检测。用siRNA277可以抑制目的基因的表达,并能阻断干扰素的信号通路。

关键词：受体干扰素αβ 克隆乙型肝炎

Dual-Roof Solar Greenhouse—A Novel Design for Improving the Heat Preserving Capacity in Northern China

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Natural Resources 2014年第12期5卷 681-686页

作者： Lilong Chai baoju wang Mingchi Liu Zhanhui Wu Yong Xu National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China Key Laboratory of Urban Agriculture (North) Ministry of Agriculture Beijing China National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China Key Laboratory of Urban Agriculture (North) Ministry of Agriculture Beijing China

Dual-roof solar greenhouse, a new style of solar greenhouse, was designed in this study intending to reduce heat loss in cold time and improve land use efficiency in Beijing, the Capital city of China. Designing and applying the dual-roof greenhouse in metropolitan area had dual effects of saving energy and enhancing land use efficiency. According to the monitoring study and analysis conducted in winter of 2012, the averaged night temperature of south room was about 12.1°C in December, which was satisfying for growing average leaf vegetables. Total energy saved by dual-roof in whole winter was quantified as 1.1 × 107 MJ.yr-1 (winter), potentially about 37.4 t coal was saved in Beijing area during whole winter-growing period. Considering the application of north room, the land use efficiency was improved by 62.5% in dual-roof solar greenhouse.

关键词： Agricultural Resource Dual-Roof Greenhouse Solar Energy Land Use Efficiency Heat Storage

Robustness Assessment of Wind Power Generation Considering Rigorous Security Constraints for Power System: A Hybrid RLO-IGDT Approach

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CSEE Journal of Power and Energy Systems 2024年第2期10卷 518-529页

作者： Lianyong Zuo Shengshi wang Yong Sun Shichang Cui Jiakun Fang Xiaomeng Ai baoju Li Chengliang Hao Jinyu Wen State Key Laboratory of Advanced Electromagnetic Technology School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430073China State Grid Jilin Electric Power Company Changchun 130021China

Fossil fuel depletion and environmental pollution problems promote development of renewable energy(RE)glob-ally.With increasing penetration of RE,operation security and economy of power systems(PS)are greatly impacted by fluctuation and intermittence of renewable power.In this paper,information gap decision theory(IGDT)is adapted to handle uncertainty of wind power generation.Based on conventional IGDT method,linear regulation strategy(LRS)and robust linear optimization(RLO)method are integrated to reformulate the model for rigorously considering security constraints.Then a robustness assessment method based on hybrid RLO-IGDT approach is proposed for analyzing robustness and economic performance of PS.Moreover,a risk-averse linearization method is adapted to convert the proposed assessment model into a mixed integer linear programming(MILP)problem for convenient optimization without robustness loss.Finally,results of case studies validate superiority of proposed method in guaranteeing operation security rigorously and effectiveness in assessment of RSR for PS without overestimation.Index Terms-Hybrid RLO-IGDT approach,information gap decision theory(IGDT),operation security,robustness assessment,robustness security region(RSR).

关键词： IGDT approach information gap decision theory(IGDT) operation security robustness assessment robustness security region(RSR)