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Molecular characterization of an outbreak-involved Bacillus anthracis strain confirms the spillover of anthrax from West Africa

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Infectious Diseases of Poverty 2024年第1期13卷 93-94页

作者： Shuchao Wang Roland Suluku Mohamed B.Jalloh Ahmed F.Samba baogui jiang Yubiao Xie Doris Harding Mengyao Zhang Foday Sahr Mahmud E.Sesay James S.Squire Mohamed A.Vandi Moinina N.Kallon Shoufeng Zhang Rongliang Hu Yuee Zhao Zhiqiang Mi Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China Department of Animal Sciences School of Agriculture and Food Sciences Njala University Njala Sierra Leone Department of Microbiology College of Medicine and Allied Health Sciences University of Sierra Leone Freetown Sierra Leone Ministry of Agriculture and Food Sciences Freetown Sierra Leone Beijing Institute of Microbiology and Epidemiology Beijing China Ministry of Health and Sanitation Freetown Sierra Leone Beijing Institute of Biotechnology Beijing China

Background Anthrax,a zoonotic disease caused by the spore-forming bacterium Bacillus anthracis,remains a major global public health concern,especially in countries with limited *** Leone,a West African country historically plagued by anthrax,has almost been out of report on this disease in recent *** this study,we described a large-scale anthrax outbreak affecting both animals and humans and attempted to characterize the *** The causative agent of the animal outbreak in Port Loko District,Sierra Leone,between March and May 2022 was identified using the nanopore sequencing technique.A nationwide active surveillance was implemented from May 2022 to June 2023 to monitor the occurrence of anthrax-specific symptoms in *** cases were subsequently verified using quantitative polymerase chain ***-genome sequencing was accomplished by combining long-read and short-read sequencing *** phylogenetic analysis was performed based on the full-chromosome single nucleotide *** The outbreak in Port Loko District,Sierra Leone,led to the death of 233 animals between March 26th and May 16th,*** ruled out the initial suspicion of Anaplasma species and successfully identified *** as the causative agent of the *** a result of the government's prompt response,out of the 49 suspected human cases identifed during the one-year active surveillance,only 6 human cases tested positive,all within the frst month after the official declaration of the *** phylogenetic analysis indicated that the BaSL2022 isolate responsible for the outbreak was positioned in the ***.153 clade within the TransEuroAsian group of *** We successfully identifed a large-scale anthrax outbreak in Sierra *** causative isolate of ***,BaSL2022,phylogenetically bridged other lineages in ***.153 clade and neighboring genetic groups,***.144 and ***.148,

关键词： Molecular identification Anthrax Bacillus anthracis Sierra Leone Phylogeny Single nucleotide polymorphism

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河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究

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中华疾病控制杂志 2024年第1期28卷 83-91页

作者：刘万双陈浩荣代科蒋宝贵陈津津田珅吴家红刘玮方立群贵州医科大学公共卫生学院流行病与卫生统计学系贵阳550025 北京化工大学生命科学与技术学院制药工程学系北京100029 军事医学研究院微生物流行病研究所病原微生物生物安全国家重点实验室北京100071 广州医科大学公共卫生学院流行病与卫生统计学系广州511436

目的通过开展发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)自然疫源地动物宿主感染新布尼亚病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)的调查研究,明确SFTS自然疫源地SFTSV的传播风险与影响因素,为SFTS的针对性防控提供科学依据。方法采用卫星定位技术连续跟踪商城县邱湾村、平塘村和龙头桥村3个村庄部分村民家养狗日常活动轨迹2周并采集血清样本,使用间接荧光抗体试验(indirect immunofluorescence assay,IFA)法检测家养狗血清SFTSV⁃IgG抗体,使用logistic回归分析模型分析影响家养狗感染SFTSV的潜在环境影响因素;采用夹(笼)夜法和粘鼠板法对家养狗活动区域内的啮齿动物及小型哺乳动物种群进行密度调查,针对保守基因进行PCR种类鉴定,并采用荧光PCR和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测啮齿动物及小型哺乳动物SFTSV感染情况。结果通过卫星定位共追踪26条家养狗的日常活动范围信息,采集37条家养狗52份血清,SFTSV⁃IgG抗体总阳性率为59.46%;共捕获啮齿动物及小型哺乳动物419只,优势种为黄胸鼠、小家鼠、黑线姬鼠,未有SFTSV阳性标本检出;家养狗活动中心离主干道路距离远和到过林地的频次多是感染SFTSV的危险因素,家养狗活动中心离林地距离远是感染SFTSV的保护因素。结论商城县家养狗SFTSV抗体阳性率较高,感染SFTSV与生态环境相关,建议进一步扩大家养动物的种类,开展SFTSV感染情况的研究;啮齿动物的SFTSV感染率低,在SFTSV自然循环过程中的作用需进一步调查。

关键词：新布尼亚病毒蜱传疾病啮齿动物血清学危险因素血小板减少综合征发热

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Isolation and in vitro cultivation of Babesia venatorum(Apicomplexa:Babesiidae),a zoonotic hemoprotozoan from Ixodes persulcatus ticks in China

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Biosafety and Health 2021年第4期3卷 210-216页

作者： Yi Sun baogui jiang Weiqing Zheng Hong Wang Ruiruo jiang Xin Wang Na Jia Fang Yang Haiying Chen Jiafu jiang Wuchun Cao State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne DiseasesNanchang Center for Disease Control and PreventionNanchang 330038China Beijing Institute of NBC Defense Beijing 102205China Qingjiangpu District Center for Disease Control and Prevention Huai'an 223002China Mudanjiang Customs of the People's Republic of China Mudanjiang 157001China

The emerging Babesia venatorum upsurges as a potential health threat occurring in China and other endemic *** attempts to isolate and culture the Babesia species had been conducted in *** collected the questing Ixodes persulcatus from identified endemic areas and allowed them to feed on the severe combined immunodeficiency(SCID)*** positive mice were chosen to provide positive erythrocytes with asexual *** for continuous culture in mouse or human erythrocytes in vitro,with RPMI 1640 medium and appropriate *** *** in the SCID mice,erythrocytes were cultured in vitro for confirmation by morphological observations with transmission electron *** of *** were then identified by way of conventional PCR *** counts monitored the growth of *** on thin blood smears and real-time quantitative PCR in *** expected,*** positive mice were harvested by sufficient attacks of *** *** erythrocytes of the infected mice were then inoculated and successfully cultured in donated erythrocytes from humans and mice in RPMI 1640 culture *** growth of *** was well demonstrated in human erythrocytes with 3.3 times parasite-load when compared with a mouse under similar *** the increased subcultures,a prolonged period of detectable parasitemia with much higher peak parasitemia and shorter time to reach peak parasitemia were observed in the following subcultures.A new strategy for isolation and in vitro culture of *** has been provided with a continuous supply of sufficient pathogens to satisfy human babesiosis's testings and clinical therapies.

关键词： Babesia Ixodes persulcatus Isolation and identification Culture characteristics China

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Outbreaks of Acute Respiratory Disease Associated with Human Adenovirus Infection in Closed Camps,China,December 2011–March 2014

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China CDC weekly 2021年第38期3卷 793-798,I0001-I0006页

作者： Juan Du Xiaodong Zhao Fang Tang Doudou Huang Guangqian Pei Xiaoai Zhang baogui jiang Qingbin Lu Wei Liu Yigang Tong Department of Laboratorial Science and Technology School of Public HealthPeking UniversityBeijingChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina Center for Diseases Control and Prevention of Chinese People’s Armed Police Forces BeijingChina The Affiliated Drum Tower Hospital of Nanjing University Medical School NanjingJiangsuChina College of Life Science and Technology Beijing University of Chemical TechnologyBeijingChina.

Summary What is already known on this topic?Human adenovirus(HAdV)was frequently associated with acute respiratory disease(ARD)outbreaks in military *** is added by this report?HAdV-B7,HAdV-B14,and HAdV-B55 were determined to be responsible for 3,2,and 4 ARD outbreaks in military camps,China,respectively,with a total attack rate of 28.0%during 2011 to 2014.

关键词： acute respiratory Acute

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The Establishment and Application of Mobile Electronic Surveillance System for Infectious Diseases with the Help of China—Sierra Leone,2016-Present

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China CDC weekly 2021年第36期3卷 763-768页

作者： Guangyu Zhao Haorong Chen Yanfeng Yan Jiafu jiang Lei Lin baogui jiang Foday Sahr Stephen Sevalie Qiang Xu Jinjin Chen Henry Saidu Bangura Kandeh Bassie Kargbo Yajun Song Wei Liu Liqun Fang Yansong Sun State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina College of Life Science and Technology Beijing University of Chemical TechnologyBeijingChina College of Medicine and Allied Health Sciences University of Sierra LeoneFreetownSierra Leone Military Hospital WilberforceFreetownSierra Leone.

Introduction:Infectious disease surveillance has long been a challenge for low-income countries like Sierra *** approaches based on paper and Short Message Service(SMS)were subject to severe delays in obtaining,transmitting,and analyzing ***:During the China aid operation for fighting Ebola since the end of 2014,a mobile electronic surveillance system for infectious diseases(MESSID)was developed in collaboration with the Republic of Sierra Leone Armed Forces(RSLAF),which comprised an Android-based reporting system and a complementary web-based program designed by Active Server ***(***)with the main functions including surveillance,real-time reporting,and risk assessment of infectious ***:MESSID was successfully registered in June 2016 and had been used by all medical and health institutions in *** June 1,2016 to July 5,2021,34,419 cases were diagnosed with 47 infectious diseases of 5 categories,with a total of 42 clinical *** to traditional approaches based on paper and SMS,the MESSID showed flexibility,high efficiency,convenience,and ***:MESSID is an accessible tool for surveillance of infectious diseases in Sierra Leone and possibly in other African countries with similar needs,capable of improving timeliness of disease reporting,thus rendering a timely outbreak detection and response.

关键词： rendering Server Sierra

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Epidemiological and Clinical Features of Patients with Scrub Typhus—Guangzhou City,Guangdong Province,China,2012–2018

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China CDC weekly 2021年第51期3卷 1079-1083,I0001-I0006页

作者： Yuehong Wei Xiugang Guan Shixia Zhou Anran Zhang Qingbin Lu Ziwei Zhou Jinjin Chen Haiyang Zhang Yang Ji baogui jiang Yang Yang Zhicong Yang Hao Li Liqun Fang Wei Liu Guangzhou Center for Disease Control and Prevention GuangzhouGuangdongChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina School of Public Health Anhui Medical UniversityHefeiAnhuiChina School of Public Health Cheeloo College of MedicineShandong UniversityJinanShandongChina Department of Laboratorial Science and Technology School of Public HealthPeking UniversityBeijingChina Department of Biostatistics College of Public Health and Health Professionsand Emerging Pathogens InstituteUniversity of FloridaFloridaUSA

Summary What is already known about this topic?Scrub typhus(ST)causes public health challenges in the“tsutsugamushi triangle”in the Asia-Pacific area greater than 13 million square kilometers,affecting an estimated one million people each *** is added by this report?A retrospective study based on 4,501 hospitalized patients with ST in Guangzhou City,China,described the epidemiological and clinical characteristics,laboratory findings of ST.

关键词： Guangzhou triangle Guangdong

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解磷解钾自生固氮菌的分离筛选及鉴定

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华中农业大学学报 2005年第1期24卷 43-48页

作者：蒋宝贵赵斌华中农业大学农业微生物国家重点实验室武汉430070

　从华中农业大学农场试验田不同土壤中分离到一系列解磷、解钾及自生固氮菌,经比较筛选得到 1组菌株PJ07、P01、HZ03、NY03,它们分别具有较强的降解难溶态的磷、分解有机磷、降解难溶态的钾和固定空气中游离态氮的能力。各菌株经鉴定... 详细信息

　从华中农业大学农场试验田不同土壤中分离到一系列解磷、解钾及自生固氮菌,经比较筛选得到 1组菌株PJ07、P01、HZ03、NY03,它们分别具有较强的降解难溶态的磷、分解有机磷、降解难溶态的钾和固定空气中游离态氮的能力。各菌株经鉴定分别属于或接近假单胞菌属(Pseudomonas)、巨大芽孢杆菌 (Bacillusmegateri um)、胶质芽孢杆菌(Bacillusmucilaginosus)和圆褐固氮菌(Azotobacterchrococcum)。统计学分析表明由各菌株组成的复合菌剂在玉米盆栽试验中可显著增加作物的总生物量。

关键词：解磷细菌解钾细菌自生固氮菌筛选与鉴定

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小学音乐教育“体验学习”的认识与实践

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家长 2020年第21期 142-143页

作者：蒋保贵甘肃省兰州市永登县城关回民小学

伴随着时代的发展与社会的进步,我国对于教学中的教学观念也发生了一定的转变,我国对于小学音乐的教学所需要的工作已经越发重视。在未来的发展中,该怎么更好地进行小学音乐教育,也就成了小学音乐教师在工作中的主要问题。为了能够进一... 详细信息

伴随着时代的发展与社会的进步,我国对于教学中的教学观念也发生了一定的转变,我国对于小学音乐的教学所需要的工作已经越发重视。在未来的发展中,该怎么更好地进行小学音乐教育,也就成了小学音乐教师在工作中的主要问题。为了能够进一步解决这项问题,体验学习这一学习方法也随之出现,而这种教学方法也能帮助小学音乐教师得到更多的教学思路启发。

关键词：小学音乐教育体验学习理论创新

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Advances in Triboelectric Nanogenerators for Blue Energy Harvesting and Marine Environmental Monitoring

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Engineering 2024年第2期33卷 204-224页

作者： Yang jiang Xi Liang Tao jiang Zhong Lin Wang CAS Center for Excellence in Nanoscience Beijing Key Laboratory of Micro–Nano Energy and SensorsBeijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijing 101400China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing 100049China Georgia Institute of Technology AtlantaGA 30332USA

Blue energy,which includes rainfall,tidal current,wave,and water-flow energy,is a promising renewable resource,although its exploitation is limited by current technologies and thus remains *** form of energy is mainly harvested by electromagnetic generators(EMGs),which generate electricity via Lorenz force-driven electron *** nano genera tors(TENGs)and TENG networks exhibit superiority over EMGs in low-frequency and high-entropy energy harvesting as a new approach for blue energy harvesting.A TENG produces electrical outputs by adopting the mechanism of Maxwell’s displacement *** date,a series of research efforts have been made to optimize the structure and performance of TENGs for effective blue energy harvesting and marine environmental *** the great progress that has been achieved in the use of TENGs in this context so far,continuous exploration is required in energy conversion,device durability,power management,and environmental *** review reports on advances in TENGs for blue energy harvesting and marine environmental *** introduces the theoretical foundations of TENGs and discusses advanced TENG prototypes for blue energy harvesting,including TENG structures that function in freestanding and contact-separation *** enhancement strategies for TENGs intended for blue energy harvesting are also ***,marine environmental applications of TENGs based on blue energy harvesting are discussed.

关键词： Triboelectric nanogenerator TENG networks Blue energy Energy harvesting Ocean sensors

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An improved initial rotor position estimation method using high-frequency pulsating voltage injection for PMSM

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Defence Technology（防务技术） 2024年第3期33卷 19-29页

作者： Yang jiang Ming Cheng School of Electrical Engineering Southeast UniversityNanjing 210096China

High frequency pulsating voltage injection method is a good candidate for detecting the initial rotor position of permanent magnet synchronous ***,traditional methods require a large number of filters,which leads to the deterioration of system stability and dynamic *** order to solve these problems,a new signal demodulation method is proposed in this *** proposed new method can directly obtain the amplitude of high-frequency current,thus eliminating the use of filters,improving system stability and dynamic performance and saving the work of adjusting filter *** addition,a new magnetic polarity detection method is proposed,which is robust to current measurement ***,experiments verify the effectiveness of the method.

关键词： Initial position detection Signal demodulation algorithm Magnetic polarity detection Filter elimination

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