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Identifying a melanogenesis-related candidate gene by a high-quality genome assembly and population diversity analysis in Hypsizygus marmoreus

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Journal of Genetics and Genomics 2021年第1期48卷 75-87页

作者： Gang Wang Lianfu Chen Weiqi Tang Yuanyuan Wang Qing Zhang Hongbo Wang Xuan Zhou Haofeng Wu Lin Guo Meijie Dou Lei Liu Baiyu Wang Jingxian Lin baogui xie Zhengchao Wang ZhongJian Liu Ray Ming Jisen Zhang Center for Genomics and Biotechnology Haixia Institute of Science and TechnologyFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCollege of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002China School of Geographical Science Nantong UniversityNantong 226001China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China Institute of Oceanography Marine Biotechnology CenterMinjiang UniversityFuzhou 350108China College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou 350002China Provincial Key Laboratory for Developmental Biology and Neurosciences College of Life SciencesFujian Normal UniversityFuzhou 350007China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray ***,very limited knowledge is known about the genomic structures and the genetic basis for the white/gray diversity of this ***,we report the near-complete high-quality *** genome at the chromosomal *** genomics analysis indicates that chromosome structures were relatively conserved,and variations in collinearity and chromosome number were mainly attributed by chromosome split/fusion events in Aragicales,whereas the fungi genome experienced many genomic chromosome fracture,fusion,and genomic replication events after the split of Aragicales from *** of 57 strains allows us to classify the population into four major groups and associate genetic variations with morphological features,indicating that white strains were not originated *** further generated genetic populations and identified a cytochrome P450 as the candidate causal gene for the melanogenesis in *** based on bulked segregant analysis (BSA)and comparative transcriptome *** high-quality *** genome and diversity data compiled in this study provide new knowledge and resources for the molecular breeding of *** as well as the evolution of Basidiomycete.

关键词： Hypsizygus marmoreus Basidiomycota P450 Bulked segregant analysis(BSA) Melanogenesis

Synthesis of Human Insulin Gene and Transformation into Tremella fuciformis

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食用菌学报 2007年第2期 9-14页

作者： xie baogui, LU Qiquan, RAO Yongbin, SUN Shujing, ZHENG Jingui Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

A human insulin gene was synthesized using PCR-based methodology, cloned into plasmid pUC18, and the sequence of the inserted fragment was confirmed. A Tremella fuciformis expression vector was constructed and used to transform T. fuciformis yeast-like conidia employing Restriction Enzyme-Mediated DNA Integration (REMI). A total of 21 antibiotic resistant colonies were selected at random of which 18 tested positive for β-glucuronidase (GUS) activity. Genomic DNA was extracted from 10 of the 21 positive transformants and PCR performed using genomic DNA as the template and specific primers for amplifying the human insulin gene, the GUS gene and the Tnos sequence. PCR data showed that the expected fragment was amplified from all 10 isolates demonstrating that they were true human insulin gene transformants.

关键词： Human insulin Gene synthesis Gene transformation Tremella fuciformis Yeast-like conidia

RNA-Seq reveals the molecular mechanism of stipe elongation in the straw mushroom Volvariella volvacea

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RNA-Seq reveals the molecular mechanism of stipe elongation ...

中国菌物学会2016年学术年会

作者： Yongxin Tao Arend F.van Peer Yuji Jiang Jian Zhu baogui xie Mycological Research Center Fujian Agriculture and Forestry University

The edible straw mushroom Volvariella volvacea is cultivated on a large scale for its unique flavor and nutritious *** characteristic five-step stages:primordia（PR）,button stage（BU）,egg stage（EG）,elongation stage（EL） and maturation stage（MA）,make it a suitable model for studies on fruiting body *** of the main factors that negatively affect yield and value is the rapid transition from EG to *** understand deep the stipe development,we analyzed genes differential expression in stipe tissue of *** using *** development turned out to be fairly complex,and regulation of stage differences is mediated mainly by changes in expression levels of genes,rather than on/off *** explicit is the strong up-regulation of cell division from BU to EG,and the very strong down-regulation hereof from EG to EL,that continues in the *** and EG share cell division as means of growth,followed by a major developmental shift towards the EL with inactivation of cell *** of regulatory genes up-regulated from EG to EL,identified three potential high upstream regulators for this ***,*** stipe elongation was the result of vertical elongation of stipe cell,not the cell *** addition,stipe elongation is related to reconstruction of stipe cell *** sixteen carbohydrate-active enzymes up-regulated during stipe elongation were *** specific expression pattern and molecular functions hinted that they could hydrolyze glucan or chitin of cell wall to participate in the reconstruction of cell wall as the ‘final operators’ during the rapid stipe *** conclusion,the new insights in stipe dynamics,together with a series of new target genes involved in stipe elongation,helps to construct a draft model of molecular mechanism of *** stipe elongation.

关键词： Volvariella volvacea Stipe elongation Cell division Cell wall degradation enzymes

来源： cnki会议评论

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金针菇菌丝与原基差异表达基因分析

食用菌学报 2014年第1期21卷 1-7页

作者：刘芳王威谢宝贵福建农林大学菌物研究中心福建福州350002

以金针菇(Flammulina velutipes)1123菌株的单孢W23基因组为参考完成其菌丝和原基转录组测序与数据分析,研究两样本间差异基因,并对差异基因进行GO功能和KEGG Pathway显著性富集分析。结果表明:两个样本中共有显著性差异表达的基因3310... 详细信息

以金针菇(Flammulina velutipes)1123菌株的单孢W23基因组为参考完成其菌丝和原基转录组测序与数据分析,研究两样本间差异基因,并对差异基因进行GO功能和KEGG Pathway显著性富集分析。结果表明:两个样本中共有显著性差异表达的基因3310个,其中在原基中上调、下调的基因数分别为1686、1624个,只在原基中表达的基因有26个。GO功能分析结果表明,在原基中膜封闭腔、内膜系统、细胞器腔、核糖核蛋白复合体、翻译调节器、发育过程、免疫系统过程、多细胞生物过程这8个GO基本单元中的差异基因全部呈现上调表达。Pathway功能富集分析结果表明,核糖体与DNA复制中的差异基因全部呈现上调表达,糖酵解途径中从D-葡萄糖转化成丙酮酸过程相关的11个差异基因全部呈下调表达,磷酸戊糖途径中相关的13个差异基因中有11个基因呈下调表达。

关键词：原基差异基因表达 GO功能 Pathway分析

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柯恩论剥削

山东科技大学学报（社会科学版） 2021年第6期23卷 104-112页

作者：谢宝贵贵州中医药大学马克思主义学院贵州贵阳550025

英国政治哲学家柯恩是当代西方分析的马克思主义学派的一位重要代表人物,他对剥削的论述突破了马克思主义对资本主义剥削的传统理解。他指出对剥削的理解无需依赖于本身就存在问题的劳动价值论;他否认了马克思主义把资本(生产资料)的不... 详细信息

英国政治哲学家柯恩是当代西方分析的马克思主义学派的一位重要代表人物,他对剥削的论述突破了马克思主义对资本主义剥削的传统理解。他指出对剥削的理解无需依赖于本身就存在问题的劳动价值论;他否认了马克思主义把资本(生产资料)的不平等分配看成是剥削产生的根源这种看法;他揭示了马克思主义的剥削理论隐含着一种自我所有权原则,并阐明了对自我所有权的限制或削弱并不一定构成剥削。尽管柯恩对剥削作了突破性的理解,但是并没有否定资本主义剥削的存在。他只是在传统马克思主义的基础上,扩展和深化了对剥削的理解和批判,进而为如何减少乃至消灭剥削指明了现实的方向。在这个意义上说,柯恩的剥削观并没有违背马克思主义的基本精神,反而可以说是发展了马克思主义。

关键词：柯恩剥削劳动价值论资本自我所有权

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应得正义观与运气平等主义

温州大学学报（社会科学版） 2022年第5期35卷 72-86页

作者：谢宝贵贵州中医药大学马克思主义学院贵州贵阳550025

在日常生活中,人们普遍持有一种朴素的应得正义观,即认为正义就在于每个人得到其所应得的。为了明确界定应得正义观,两种不同的“应得”概念被区分开来:前制度性应得与制度性应得。应得正义观一贯使用的应得概念指的是前制度性应得。在... 详细信息

在日常生活中,人们普遍持有一种朴素的应得正义观,即认为正义就在于每个人得到其所应得的。为了明确界定应得正义观,两种不同的“应得”概念被区分开来:前制度性应得与制度性应得。应得正义观一贯使用的应得概念指的是前制度性应得。在当代,这种正义观遭到了罗尔斯的质疑,他认为应得正义观是不适用的和不可行的。虽然罗尔斯对应得正义观的质疑是站不住脚的,但它却促成了运气平等主义。从表面上来看,运气平等主义不但没有否定应得正义观,而且似乎还是对它的一种扩展,但是对二者关系的深入考察表明它们在本质上并不一致。此外,运气平等主义作为一种正义观存在着一个重要缺陷,即它忽略了生产资料私有制所造成的巨大不平等;而应得正义观则不存在这样的缺陷,显得更为合理。

关键词：应得应得正义观罗尔斯平等运气平等主义

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对平等主义的两个挑战:优先主义与充足主义

山东科技大学学报（社会科学版） 2017年第1期19卷 82-90页

作者：谢宝贵贵州师范学院马列主义教学部贵州贵阳550018

平等主义在当代政治哲学中已成为一种重要的分配正义观,但是还有另外两种学说对之构成了两个挑战,它们就是优先主义和充足主义。尽管这两种学说各自的侧重点不同,但是它们至少有一点是一致的:它们都不承认平等本身是好的。平等主义区别... 详细信息

平等主义在当代政治哲学中已成为一种重要的分配正义观,但是还有另外两种学说对之构成了两个挑战,它们就是优先主义和充足主义。尽管这两种学说各自的侧重点不同,但是它们至少有一点是一致的:它们都不承认平等本身是好的。平等主义区别于它们的本质之处就在于,平等主义承认平等本身就是好的,因而具有内在价值。在对优先主义与充足主义进行了若干分析与批判之后,它们对平等主义的挑战就显得站不住脚。基于比较性公平的观点,可以比较合理地论证平等的内在价值。而且,从这一论证中,还可以引出一种"比例性平等观",它可以成为平等主义所要追求的新目标。

关键词：平等平等主义优先主义充足主义比例性平等