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应得正义观与运气平等主义

温州大学学报（社会科学版） 2022年第5期35卷 72-86页

作者：谢宝贵贵州中医药大学马克思主义学院贵州贵阳550025

在日常生活中,人们普遍持有一种朴素的应得正义观,即认为正义就在于每个人得到其所应得的。为了明确界定应得正义观,两种不同的“应得”概念被区分开来:前制度性应得与制度性应得。应得正义观一贯使用的应得概念指的是前制度性应得。在... 详细信息

在日常生活中,人们普遍持有一种朴素的应得正义观,即认为正义就在于每个人得到其所应得的。为了明确界定应得正义观,两种不同的“应得”概念被区分开来:前制度性应得与制度性应得。应得正义观一贯使用的应得概念指的是前制度性应得。在当代,这种正义观遭到了罗尔斯的质疑,他认为应得正义观是不适用的和不可行的。虽然罗尔斯对应得正义观的质疑是站不住脚的,但它却促成了运气平等主义。从表面上来看,运气平等主义不但没有否定应得正义观,而且似乎还是对它的一种扩展,但是对二者关系的深入考察表明它们在本质上并不一致。此外,运气平等主义作为一种正义观存在着一个重要缺陷,即它忽略了生产资料私有制所造成的巨大不平等;而应得正义观则不存在这样的缺陷,显得更为合理。

关键词：应得应得正义观罗尔斯平等运气平等主义

Molecular characterization of an outbreak-involved Bacillus anthracis strain confirms the spillover of anthrax from West Africa

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Infectious Diseases of Poverty 2024年第1期13卷 93-94页

作者： Shuchao Wang Roland Suluku Mohamed B.Jalloh Ahmed F.Samba baogui Jiang Yubiao xie Doris Harding Mengyao Zhang Foday Sahr Mahmud E.Sesay James S.Squire Mohamed A.Vandi Moinina N.Kallon Shoufeng Zhang Rongliang Hu Yuee Zhao Zhiqiang Mi Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China Department of Animal Sciences School of Agriculture and Food Sciences Njala University Njala Sierra Leone Department of Microbiology College of Medicine and Allied Health Sciences University of Sierra Leone Freetown Sierra Leone Ministry of Agriculture and Food Sciences Freetown Sierra Leone Beijing Institute of Microbiology and Epidemiology Beijing China Ministry of Health and Sanitation Freetown Sierra Leone Beijing Institute of Biotechnology Beijing China

Background Anthrax,a zoonotic disease caused by the spore-forming bacterium Bacillus anthracis,remains a major global public health concern,especially in countries with limited *** Leone,a West African country historically plagued by anthrax,has almost been out of report on this disease in recent *** this study,we described a large-scale anthrax outbreak affecting both animals and humans and attempted to characterize the *** The causative agent of the animal outbreak in Port Loko District,Sierra Leone,between March and May 2022 was identified using the nanopore sequencing technique.A nationwide active surveillance was implemented from May 2022 to June 2023 to monitor the occurrence of anthrax-specific symptoms in *** cases were subsequently verified using quantitative polymerase chain ***-genome sequencing was accomplished by combining long-read and short-read sequencing *** phylogenetic analysis was performed based on the full-chromosome single nucleotide *** The outbreak in Port Loko District,Sierra Leone,led to the death of 233 animals between March 26th and May 16th,*** ruled out the initial suspicion of Anaplasma species and successfully identified *** as the causative agent of the *** a result of the government's prompt response,out of the 49 suspected human cases identifed during the one-year active surveillance,only 6 human cases tested positive,all within the frst month after the official declaration of the *** phylogenetic analysis indicated that the BaSL2022 isolate responsible for the outbreak was positioned in the ***.153 clade within the TransEuroAsian group of *** We successfully identifed a large-scale anthrax outbreak in Sierra *** causative isolate of ***,BaSL2022,phylogenetically bridged other lineages in ***.153 clade and neighboring genetic groups,***.144 and ***.148,

关键词： Molecular identification Anthrax Bacillus anthracis Sierra Leone Phylogeny Single nucleotide polymorphism

Identifying a melanogenesis-related candidate gene by a high-quality genome assembly and population diversity analysis in Hypsizygus marmoreus

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Journal of Genetics and Genomics 2021年第1期48卷 75-87页

作者： Gang Wang Lianfu Chen Weiqi Tang Yuanyuan Wang Qing Zhang Hongbo Wang Xuan Zhou Haofeng Wu Lin Guo Meijie Dou Lei Liu Baiyu Wang Jingxian Lin baogui xie Zhengchao Wang ZhongJian Liu Ray Ming Jisen Zhang Center for Genomics and Biotechnology Haixia Institute of Science and TechnologyFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCollege of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002China School of Geographical Science Nantong UniversityNantong 226001China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China Institute of Oceanography Marine Biotechnology CenterMinjiang UniversityFuzhou 350108China College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou 350002China Provincial Key Laboratory for Developmental Biology and Neurosciences College of Life SciencesFujian Normal UniversityFuzhou 350007China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray ***,very limited knowledge is known about the genomic structures and the genetic basis for the white/gray diversity of this ***,we report the near-complete high-quality *** genome at the chromosomal *** genomics analysis indicates that chromosome structures were relatively conserved,and variations in collinearity and chromosome number were mainly attributed by chromosome split/fusion events in Aragicales,whereas the fungi genome experienced many genomic chromosome fracture,fusion,and genomic replication events after the split of Aragicales from *** of 57 strains allows us to classify the population into four major groups and associate genetic variations with morphological features,indicating that white strains were not originated *** further generated genetic populations and identified a cytochrome P450 as the candidate causal gene for the melanogenesis in *** based on bulked segregant analysis (BSA)and comparative transcriptome *** high-quality *** genome and diversity data compiled in this study provide new knowledge and resources for the molecular breeding of *** as well as the evolution of Basidiomycete.

关键词： Hypsizygus marmoreus Basidiomycota P450 Bulked segregant analysis(BSA) Melanogenesis

壳聚糖和ε-聚赖氨酸处理对双孢蘑菇贮藏过程中品质的影响

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菌物学报 2022年第1期41卷 146-154页

作者：魏奇翁馨李婕刘盛荣张维瑞谢宝贵江玉姬宁德师范学院生命科学学院福建宁德352100 福建农林大学食品科学学院福建福州350002 福建农林大学菌物研究中心福建福州350002

以延长双孢蘑菇货架期为目标,探讨壳聚糖和ε-聚赖氨酸处理对采后双孢蘑菇在4℃贮藏过程中生理特性、营养品质和贮藏特性的影响。结果表明:与对照组双孢蘑菇相比,壳聚糖与ε-聚赖氨酸6:4复配溶液处理能够有效抑制双孢蘑菇表面微生物的... 详细信息

以延长双孢蘑菇货架期为目标,探讨壳聚糖和ε-聚赖氨酸处理对采后双孢蘑菇在4℃贮藏过程中生理特性、营养品质和贮藏特性的影响。结果表明:与对照组双孢蘑菇相比,壳聚糖与ε-聚赖氨酸6:4复配溶液处理能够有效抑制双孢蘑菇表面微生物的生长和多酚氧化酶活性的增加,保持双孢蘑菇子实体较高的L*值和硬度,延缓双孢蘑菇的质量损失和细胞膜透性的升高,减少双孢蘑菇子实体的腐烂,保持较高商品率。在4℃条件下,壳聚糖与ε-聚赖氨酸6:4复配溶液对双孢蘑菇的保鲜性能最优,能够有效保持双孢蘑菇的商品品质和延长其贮藏时间。

关键词：双孢蘑菇壳聚糖 ε-聚赖氨酸贮藏特性

Synthesis of Human Insulin Gene and Transformation into Tremella fuciformis

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食用菌学报 2007年第2期 9-14页

作者： xie baogui, LU Qiquan, RAO Yongbin, SUN Shujing, ZHENG Jingui Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

A human insulin gene was synthesized using PCR-based methodology, cloned into plasmid pUC18, and the sequence of the inserted fragment was confirmed. A Tremella fuciformis expression vector was constructed and used to transform T. fuciformis yeast-like conidia employing Restriction Enzyme-Mediated DNA Integration （REMI）. A total of 21 antibiotic resistant colonies were selected at random of which 18 tested positive for β-glucuronidase （GUS） activity. Genomic DNA was extracted from 10 of the 21 positive transformants and PCR performed using genomic DNA as the template and specific primers for amplifying the human insulin gene, the GUS gene and the Tnos sequence. PCR data showed that the expected fragment was amplified from all 10 isolates demonstrating that they were true human insulin gene transformants.

关键词： Human insulin Gene synthesis Gene transformation Tremella fuciformis Yeast-like conidia

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奶油绚孔菌菌丝多糖的提取及其抗氧化活性

菌物学报 2022年第12期41卷 2036-2047页

作者：魏奇韩蓉严晶黄骏张维瑞刘盛荣谢宝贵江玉姬宁德师范学院生命科学学院福建宁德352100 闽东特色生物资源福建省高校工程研究中心福建宁德352100 福建农林大学菌物研究中心福建福州350002 福建农林大学食品科学学院福建福州350002

为了获得奶油绚孔菌菌丝多糖的最佳制备工艺并进一步评估其体外抗氧化的作用,本研究以奶油绚孔菌菌丝作为原料,采用中心组合试验法结合响应面分析法对奶油绚孔菌菌丝多糖的提取条件进行优化。结果表明,最佳的提取工艺为:料液比1:32、提... 详细信息

为了获得奶油绚孔菌菌丝多糖的最佳制备工艺并进一步评估其体外抗氧化的作用,本研究以奶油绚孔菌菌丝作为原料,采用中心组合试验法结合响应面分析法对奶油绚孔菌菌丝多糖的提取条件进行优化。结果表明,最佳的提取工艺为:料液比1:32、提取时间1.85h和提取温度85℃,奶油绚孔菌菌丝多糖在此提取工艺条件下的得率为5.68%。奶油绚孔菌菌丝多糖对DPPH、ABTS和羟基自由基有清除能力,其EC50分别为3.74、3.80和15.10 mg/mL。奶油绚孔菌菌丝多糖呈现出一定的还原能力。由此可知,奶油绚孔菌菌丝多糖具有一定的体外抗氧化作用,该研究能够为奶油绚孔菌菌丝多糖的进一步综合开发提供理论支持。

关键词：奶油绚孔菌抗氧化活性多糖

基于二代测序技术的MNP标记鉴别刺芹侧耳菌株

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食用菌学报 2023年第1期30卷 1-9页

作者：魏传正王朦张鹏刘芳严俊杰谢宝贵邓优锦谢路昱福建农林大学菌物研究中心福建福州350002 中国农业科学院都市农业研究所四川成都610213 新加坡国立大学计算机学院肯特岗117417

以31个刺芹侧耳(Pleurotus eryngii)菌株及福建省主栽品种‘Ple 0100’为材料,经二代测序与标记序列筛选,构建32个菌株的MNP标记数据库,结果表明,32个菌株之间的遗传相似度为1.79%~99.60%,其中DUS16与DUS17的遗传相似度为99.60%,DUS02与... 详细信息

以31个刺芹侧耳(Pleurotus eryngii)菌株及福建省主栽品种‘Ple 0100’为材料,经二代测序与标记序列筛选,构建32个菌株的MNP标记数据库,结果表明,32个菌株之间的遗传相似度为1.79%~99.60%,其中DUS16与DUS17的遗传相似度为99.60%,DUS02与DUS23、DUS30的遗传相似度均为99.01%,DUS23与DUS30的遗传相似度为99.20%。拮抗实验结果表明,遗传相似度高的菌株之间无拮抗现象,可能是极近似品种或相同品种。对3家刺芹侧耳生产企业的子实体、子实体组织分离物、废菌渣、菇脚进行MNP检测,发现这些样品与‘Ple 0100’的遗传相似度均为100.00%,表明利用MNP标记进行品种鉴别的材料不限于菌丝体。对5个自交菌株及其亲本进行MNP标记检测,结果表明,自交菌株之间遗传相似度为26.84%~61.43%,与亲本之间的遗传相似度为28.43%~78.33%,表明刺芹侧耳减数分裂过程中存在广泛的染色体重组和同源染色体交换。对国内52家刺芹侧耳生产企业的56份鲜菇样品的组织分离物进行MNP检测,发现与‘Ple 0100’的相似度均为100.00%,表明国内刺芹侧耳栽培品种高度一致。研究结果表明基于二代测序技术的MNP标记技术可用于刺芹侧耳菌株间遗传相似度分析及新菌株鉴别。

关键词：遗传相似度品种鉴定二代测序多聚单核苷酸多态性拮抗反应

RNA-Seq reveals the molecular mechanism of stipe elongation in the straw mushroom Volvariella volvacea

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RNA-Seq reveals the molecular mechanism of stipe elongation ...

中国菌物学会2016年学术年会

作者： Yongxin Tao Arend F.van Peer Yuji Jiang Jian Zhu baogui xie Mycological Research Center Fujian Agriculture and Forestry University

The edible straw mushroom Volvariella volvacea is cultivated on a large scale for its unique flavor and nutritious *** characteristic five-step stages:primordia（PR）,button stage（BU）,egg stage（EG）,elongation stage（EL） and maturation stage（MA）,make it a suitable model for studies on fruiting body *** of the main factors that negatively affect yield and value is the rapid transition from EG to *** understand deep the stipe development,we analyzed genes differential expression in stipe tissue of *** using *** development turned out to be fairly complex,and regulation of stage differences is mediated mainly by changes in expression levels of genes,rather than on/off *** explicit is the strong up-regulation of cell division from BU to EG,and the very strong down-regulation hereof from EG to EL,that continues in the *** and EG share cell division as means of growth,followed by a major developmental shift towards the EL with inactivation of cell *** of regulatory genes up-regulated from EG to EL,identified three potential high upstream regulators for this ***,*** stipe elongation was the result of vertical elongation of stipe cell,not the cell *** addition,stipe elongation is related to reconstruction of stipe cell *** sixteen carbohydrate-active enzymes up-regulated during stipe elongation were *** specific expression pattern and molecular functions hinted that they could hydrolyze glucan or chitin of cell wall to participate in the reconstruction of cell wall as the ‘final operators’ during the rapid stipe *** conclusion,the new insights in stipe dynamics,together with a series of new target genes involved in stipe elongation,helps to construct a draft model of molecular mechanism of *** stipe elongation.

关键词： Volvariella volvacea Stipe elongation Cell division Cell wall degradation enzymes

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金针菇菌丝与原基差异表达基因分析

食用菌学报 2014年第1期21卷 1-7页

作者：刘芳王威谢宝贵福建农林大学菌物研究中心福建福州350002

以金针菇(Flammulina velutipes)1123菌株的单孢W23基因组为参考完成其菌丝和原基转录组测序与数据分析,研究两样本间差异基因,并对差异基因进行GO功能和KEGG Pathway显著性富集分析。结果表明:两个样本中共有显著性差异表达的基因3310... 详细信息

以金针菇(Flammulina velutipes)1123菌株的单孢W23基因组为参考完成其菌丝和原基转录组测序与数据分析,研究两样本间差异基因,并对差异基因进行GO功能和KEGG Pathway显著性富集分析。结果表明:两个样本中共有显著性差异表达的基因3310个,其中在原基中上调、下调的基因数分别为1686、1624个,只在原基中表达的基因有26个。GO功能分析结果表明,在原基中膜封闭腔、内膜系统、细胞器腔、核糖核蛋白复合体、翻译调节器、发育过程、免疫系统过程、多细胞生物过程这8个GO基本单元中的差异基因全部呈现上调表达。Pathway功能富集分析结果表明,核糖体与DNA复制中的差异基因全部呈现上调表达,糖酵解途径中从D-葡萄糖转化成丙酮酸过程相关的11个差异基因全部呈下调表达,磷酸戊糖途径中相关的13个差异基因中有11个基因呈下调表达。

关键词：原基差异基因表达 GO功能 Pathway分析