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低钠配方对酱牦牛肉食用品质及氧化特性的影响

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食品工业科技 2024年第14期45卷 62-70页

作者：王雨祺许必锋蔡克周徐宝才王秋雨张树林兰道亮王琳琳西南民族大学食品科学与技术学院四川成都610041 中国安徽六安索伊电器制造有限公司安徽六安237000 合肥工业大学食品与生物工程学院安徽合肥230009 西南民族大学畜牧兽医学院四川成都610041

为了探究不同低钠配方对牦牛肉制品食用品质及氧化特性的影响,明确酱牦牛肉制品腌制的最佳复合盐比例。本试验以牦牛前腿肉为主要原料,添加不同比例的氯化钾(KCl)和焦磷酸钠替代部分NaCl对肉样进行腌制,分析不同比例低钠复合盐对酱牦牛... 详细信息

为了探究不同低钠配方对牦牛肉制品食用品质及氧化特性的影响,明确酱牦牛肉制品腌制的最佳复合盐比例。本试验以牦牛前腿肉为主要原料,添加不同比例的氯化钾(KCl)和焦磷酸钠替代部分NaCl对肉样进行腌制,分析不同比例低钠复合盐对酱牦牛肉出品率、食用品质、脂质氧化和蛋白氧化等各项指标的影响,并优化得到钠盐复合替代物最佳复配比例。结果表明,随着焦磷酸钠和KCl替代量的不断增加,产品的出品率显著增加(P<0.05),肉色及其稳定性显著提高(P<0.05);与完全使用NaCl腌制的空白组相比各组TBARS值均呈显著降低趋势(P<0.05),各组羰基含量均低于空白组,且呈显著下降趋势(P<0.05),巯基含量的下降受到显著抑制(P<0.05),脂质氧化和蛋白氧化程度被显著抑制(P<0.05)。综上所述,利用焦磷酸钠和KCl替代量部分NaCl时,酱牦牛肉的食用品质提升、氧化程度降低,当复合盐比例为NaCl含量50%、KCl含量30%、焦磷酸钠含量20%时效果最佳。本研究结果为改善低钠牦牛肉制品品质提供了一定数据支持和新的思路。

关键词：牦牛肉低钠配方钠盐替代品质特性氧化特性

维普期刊数据库博看期刊

利用陆海杂种BC_1群体构建棉花遗传连锁图谱并初步定位产量性状相关的QTL

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中国农学通报 2009年第9期25卷 11-18页

作者：李骏智杨泽茂李俊文石玉真刘爱英陈琴李爱国张保才刘广平蒋建雄王涛袁有禄湖南农业大学生物科学技术学院长沙410128 中国农业科学院棉花研究所农业部棉花遗传改良重点实验室河南安阳455004 湖北长江大学农学院湖北荆州434025

利用陆地棉推广品种中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建回交群体（BC1F1,BC2F1和BC1S1）。用亲本和F1对新开发的2102对SSR引物进行多态性筛选,共筛选到317对含有海1显性带的引物,占筛选引物总数的15.08%;最终对其中... 详细信息

利用陆地棉推广品种中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建回交群体（BC1F1,BC2F1和BC1S1）。用亲本和F1对新开发的2102对SSR引物进行多态性筛选,共筛选到317对含有海1显性带的引物,占筛选引物总数的15.08%;最终对其中的275对引物进行了BC1F1群体扩增,获得306个SSR标记差异位点。连锁分析表明（LOD=6.5）,有254个标记位点连锁,分布在42个连锁群中,覆盖2252.36cM,约占棉花基因组的50.05%;平均每个连锁群有6.08个标记,覆盖53.63cM;标记间平均间距为8.87cM。利用BC1F1、BC2F1和BC1S1三个不同世代分离群体产量性状数据,共定位16个产量性状QTL,解释表型变异5.77%~19.86%。其中,衣分6个,铃重6个,籽指4个。有9个增效基因来自陆地棉亲本中棉所36,7个增效基因来自海岛棉亲本海1,说明了表型性状较差的品种同样可能含有可用于性状改良的增效基因。控制衣分的3个QTL可在不同的世代稳定检测到,效应稳定,增效基因均来自高值亲本陆地棉,为进一步分子标记辅助选择奠定了基础。

关键词：陆海回交群体遗传图谱产量性状 QTL

全乳切除术后放射治疗对前哨淋巴结微转移早期乳腺癌患者预后影响的Meta分析

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东南大学学报（医学版） 2024年第1期43卷 97-104页

作者：格桑卓嘎韩丽飞王宝偲张亚男胡浩霖东南大学医学院江苏南京210009 东南大学附属中大医院乳腺病诊疗中心江苏南京210009

目的:荟萃文献,对比分析前哨淋巴结微转移早期乳腺癌患者全乳切除术后行与未行放射治疗的预后。方法:检索中国生物医学文献数据库(China Biology Medicine,CBM)、中国期刊全文数据库(China National Knowledge Infrastructure,CNKI)、维... 详细信息

目的:荟萃文献,对比分析前哨淋巴结微转移早期乳腺癌患者全乳切除术后行与未行放射治疗的预后。方法:检索中国生物医学文献数据库(China Biology Medicine,CBM)、中国期刊全文数据库(China National Knowledge Infrastructure,CNKI)、维普(VIP)、万方数据库、PubMed数据库、Embase数据库、Web of science(WOS)数据库、Cochrane数据库,由2名研究者各自独立对入选研究的有关试验设计、研究对象特征、研究结果等进行摘录,采用纽卡斯尔渥太华量表进行质量评价,采用Stata 17.0及Review Manager 5.4进行Meta分析。结果:最终筛选出9篇相关文献,均为回顾性研究。中位随访30~98个月。术后放射治疗组与非放射治疗组乳腺癌特异性生存期(breast cancer-specific survival,BCSS)差异无统计学意义(HR=1.18,95%CI:0.94~1.48,Z=1.43,P=0.15);两组无病生存期(disease-free survival,DFS)差异亦无统计学意义(HR=0.51,95%CI:0.07~3.62,Z=-0.668,P=0.50);两组总生存期(overall survival,OS)差异亦无统计学意义(HR=1.02,95%CI:0.93~1.12,Z=0.39,P=0.70)。结论:前哨淋巴结微转移早期乳腺癌患者(T0、T1~2NmiM0)全乳切除术后予以放射治疗并不能改善患者的BCSS、DFS、OS。

关键词：乳腺肿瘤前哨淋巴结微转移全乳切除术放射治疗 Meta分析

基于超声熔盐复合清洗的多物理场仿真研究及其关键参数优化

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工具技术 2018年第12期52卷 49-53页

作者：杨明斌贾秀杰李方义孙一航张保财辛本礼王光存山东大学机械工程国家级实验教学示范中心(山东大学) 江苏徐工工程机械研究院有限公司

根据前期调研并结合再制造清洗对象特点,建立了适用于再制造毛坯件超声熔盐复合清洗的三维仿真模型,并根据清洗位置范围以及全面客观地反映清洗槽内声场分布,合理选取了声场评价面。运用Comsol Multiphysics软件对300℃温度场中不同超... 详细信息

根据前期调研并结合再制造清洗对象特点,建立了适用于再制造毛坯件超声熔盐复合清洗的三维仿真模型,并根据清洗位置范围以及全面客观地反映清洗槽内声场分布,合理选取了声场评价面。运用Comsol Multiphysics软件对300℃温度场中不同超声振子排布及频率参数条件下的声场分布进行了仿真研究,根据得到的仿真结果选择最优复合清洗参数。最后,通过铝箔腐蚀法验证了仿真结果的准确性,对于促进再制造产业发展,提升行业再制造水平具有指导意义。

关键词：再制造超声熔盐复合清洗声场分布仿真研究

Does not hUTP14a promoter form a regulation feedback loop with P53?

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Chinese Journal of Cancer Research 2014年第2期26卷 159-165页

作者： Jingyi zhang Yafei Guo Xiaojuan Du baocai Xing Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Hepatic Biliary & Pancreatic Surgery IPeking University Cancer Hospital & Institute Department of Cell Biology Peking University Health Science Center

Objective： We previously found that hUTP14a binds P53 and promotes P53 degradation. However, if hUTP14a is a downstream gene of P53 remains to be determined. This study aimed to identify the promoter of h UTP14a and investigate if h UTP14a is regulated by P53. Methods： The hUTPI4a promoter region was cloned into pGL3-Basic-luciferase reporter plasmid to get pGL3-hUTP14a-luc. The reporter plasmid was transfected into 293T cells and luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Putative transcription factors were identified through searching Matlnspector Professional and Algorismica i Genetica databases. Either pGL3-hUTPI4aluc or p21 promoter reporter plasmid was co-transfected with increasing dose of p53 plasmid, and luciferase activity was evaluated. A series of deletion constructs of pGL3-hUTP14a-luc were constructed and minimal promoter region of hUTP14a was determined. Differences of the lnciferase activities between different groups were assessed by statistical analysis. Results： The hUTP14a gene promoter reporter construct was correctly cloned and was demonstrated to possess promoter activity. The transcription of hUTP14a was not regulated by P53. The minimal promoter region of h UTP14a gene is located between -203 to -100 of the transcription initiation site. Conclusion： Unlike other P53-interacting proteins such as MDM2, Pirh2 and Cop I which promote P53 degradation and whose transcriptions are regulated by P53, does not hUTP14a transcription form a regulation feedback loop with P 53.

关键词： hUTP 14a luciferase activity promoter P5 3 transcription factor

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福建省山药种质资源品质比较与综合评价

食品研究与开发 2024年第9期45卷 75-85页

作者：张武君刘保财陈菁瑛黄颖桢赵云青福建省农业科学院作物研究所/福建省农业科学院药用植物研究中心福建福州350013

该研究较为全面地收集福建省6个地市45份山药主栽种质资源,测定块茎的9个品质指标,利用隶属函数法、主成分分析和聚类分析对山药的营养品质、加工品质和综合品质进行评价。结果表明,测定的9个指标中,淀粉含量和可食率的变异系数低于8%,... 详细信息

该研究较为全面地收集福建省6个地市45份山药主栽种质资源,测定块茎的9个品质指标,利用隶属函数法、主成分分析和聚类分析对山药的营养品质、加工品质和综合品质进行评价。结果表明,测定的9个指标中,淀粉含量和可食率的变异系数低于8%,其余7个指标的变异系数大于20%,其中块茎鲜重的变异系数高达59.90%。筛选出可溶性糖含量、淀粉含量、可溶性蛋白含量、尿囊素含量、折干率、可食率、块茎鲜重7项指标,构建营养品质和加工品质相结合的品质综合评价体系。聚类分析将福建山药种质资源聚为4类,第Ⅰ类营养和加工品质较一般;第Ⅱ类适合加工;第Ⅲ类适合鲜食;第Ⅳ类鲜食销售和加工均有优势。

关键词：山药资源营养加工综合评价

AB-QTL法定位陆海杂种棉花产量相关性状

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分子植物育种 2008年第3期6卷 504-510页

作者：李爱国张保才李俊文石玉真刘爱英杨泽茂刘志余筱南袁有禄湖南农业大学农学院长沙410128 中国农业科学院棉花研究所农业部棉花遗传改良重点实验室安阳455000 湖南农业大学生物科学技术学院长沙410128

本研究利用中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建高代回交群体。利用3223对SSR引物筛选亲本和F1,筛选到294对含有海1显性带的引物,占筛选引物总数的9.12%。最终对其中的267对引物进行了群体扩增,获得277个SSR标记差异... 详细信息

本研究利用中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建高代回交群体。利用3223对SSR引物筛选亲本和F1,筛选到294对含有海1显性带的引物,占筛选引物总数的9.12%。最终对其中的267对引物进行了群体扩增,获得277个SSR标记差异位点。其中,217个标记位点连锁,构建44个连锁群,平均每个连锁群包含4.93个标记位点。图谱覆盖1908.67cM,占棉花基因组的42.89%,平均每个连锁群覆盖43.38cM,标记间平均相距8.80cM。利用陆海杂种BC1F1、BC2F1和BC1S1的表型数据,通过复合区间作图,共检测到9个QTL,解释表型变异6.90%￣19.17%。其中3个增效基因来自海岛棉亲本海1,6个增效基因来自陆地棉亲本中棉所36。此外,控制衣分的2个QTL在3个世代都能够检测到,分别解释6.90%和19.17%的表型变异,效应稳定,为进一步分子标记辅助选择奠定了基础。

关键词：连锁图谱产量性状 AB-QTL

Multi-omics Analysis of Primary Cell Culture Models Reveals Genetic and Epigenetic Basis of Intratumoral Phenotypic Diversity

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Genomics, Proteomics & Bioinformatics 2019年第6期17卷 576-589页

作者： Sixue Liu Zuyu Yang Guanghao Li Chunyan Li Yanting Luo Qiang Gong Xin Wu Tao Li Zhiqian zhang baocai Xing Xiaolan Xu Xuemei Lu CAS Key Laboratory of Genomics and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Invasive Pathogens Laboratory Institute of Environmental Science and ResearchPorirua 5022WellingtonNew Zealand Department of Cell Biology Key Laboratory of Carcinogenesis and Translational ResearchCenter for Molecular and Translational MedicinePeking University Cancer Hospital and InstituteBeijing 100142China Department of Hepatobiliary Surgery I Peking University Cancer Hospital and InstituteBeijing 100142China National Key Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

Uncovering the functionally essential variations related to tumorigenesis and tumor progression from cancer genomics data is still challenging due to the genetic diversity among patients,and extensive inter-and intra-tumoral heterogeneity at different levels of gene expression regulation,including but not limited to the genomic, epigenomic, and transcriptional levels. To minimize the impact of germline genetic heterogeneities, in this study, we establish multiple primary cultures from the primary and recurrent tumors of a single patient with hepatocellular carcinoma(HCC). Multiomics sequencing was performed for these cultures that encompass the diversity of tumor cells from the same patient. Variations in the genome sequence, epigenetic modification, and gene expression are used to infer the phylogenetic relationships of these cell cultures. We find the discrepancy among the relationships revealed by single nucleotide variations(SNVs) and transcriptional/epigenomic profiles from the cell cultures. We fail to find overlap between sample-specific mutated genes and differentially expressed genes(DEGs), suggesting that most of the heterogeneous SNVs among tumor stages or lineages of the patient are functionally insignificant. Moreover, copy number alterations(CNAs) and DNA methylation variation within gene bodies, rather than promoters, are significantly correlated with gene expression variability among these cell cultures. Pathway analysis of CNA/DNA methylation-related genes indicates that a single cell clone from the recurrent tumor exhibits distinct cellular characteristics and tumorigenicity, and such an observation is further confirmed by cellular experiments both in vitro and in vivo. Our systematic analysis reveals that CNAs and epigenomic changes, rather than SNVs, are more likely to contribute to the phenotypic diversity among subpopulations in the tumor. These findings suggest that new therapeutic strategies targeting gene dosage and epigenetic modification s

关键词： Intra-individual tumor heterogeneity Parallel primary culture Multi-omics Functional phenotypic diversity

Non-fullerene acceptor pre-aggregates enable high efficiency pseudo-bulk heterojunction organic solar cells

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Science China Chemistry 2022年第2期65卷 373-381页

作者： Donghui Li Chuanhang Guo Xue zhang baocai Du Cong Yu Pang Wang Shili Cheng Liang Wang Jinlong Cai Hui Wang Dan Liu Huifeng Yao Yanming Sun Jianhui Hou Tao Wang School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China State Key Laboratory of Polymer Physics and Chemistry Beijing National Laboratory for Molecular SciencesCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China School of Chemistry Beihang UniversityBeijing 100191China

Pseudo-bulk heterojunction(BHJ)fabricated by sequential casting of donor and acceptor layers has been recently demonstrated as a superior structure to prepare organic solar cells(OSCs)with enhanced efficiency compared to the conventional BHJ OSCs cast from a common solution of donors and ***,molecular diffusion and aggregation within the pseudo-BHJ layer bring great challenges to fully realize the advantage of pseudo-BHJ ***,a solution-incubated pre-aggregation strategy is employed to tune the nanoscale aggregates of non-fullerene acceptor(NFA)BTP-e C11 and N3 to substantially enhance device power-conversion efficiency(PCE).NFA pre-aggregates are incubated in solutions via aging or adding antisolvent,and then sequentially cast onto D18 fibrillar network,which then penetrate to form a pseudo-BHJ structure with appropriate domain sizes to ensure superior charge *** the conventional pseudo-BHJ OSCs obtain inferior PCEs below 17%compared with normal BHJ OSCs,NFA pre-aggregates help to achieve remarkable PCEs of 17.7%and 17.5%for D18/BTP-e C11 and D18/N3 pseudo-BHJ *** work demonstrates that the solution-incubated nanoscale pre-aggregation is an efficient approach to regulate molecular diffusion and aggregation to guarantee high performance pseudo-BHJ OSCs.

关键词： non-fullerene acceptors pre-aggregate pseudo-bulk heterojunction organic solar cells