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Uncovering responsive miRNAs in tea plant(Camellia sinensis)against leaf spot infected by Phoma segeticola *** using high-throughput sequencing and prediction of their targets through degradome

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Uncovering responsive miRNAs in tea plant(Camellia sinensis)...

中国植物病理学会2019年学术年会

作者： Li Dongxue Wang Xue Yin Qiaoxiu Ren Yafeng Bao Xingtao Dharmasena Dissanayake-saman-pradeep Wu Xian song baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University

Tea plant(Camellia sinensis) was widely grown in Guizhou Province,in China,with the grown area reached at the top *** leaf spot caused by Phoma segeticola *** was firstly reported in tea plantation in Guizhou Province,which lead to a huge loss of tea *** response mechanism of tea host under the stress of the pathogen would contribute for the future research on hostpathogen interactions,determination of trait-specific genes and plant-host adaptation mechanisms.970 known miRNAs or conservative miRNAs and 4730 novel miRNAs were identified from two treatment groups inoculated by Phoma segeticola *** and health tea *** with the treatment group of inoculation pathogen and the healthy treatment group,the number of miRNAs with up-regulated trends or down-regulated trends was 17 and 15 at P<***-MIR6149 a-p3

关键词： Biotic stress Plant-pathogen interaction miRNA Target genes molecular mechanism

The Identification of Tea Gray Blight Disease in Huishui County,Guizhou Province,China

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The Identification of Tea Gray Blight Disease in Huishui Cou...

中国植物病理学会2017年学术年会

作者： Li Dongxue Zhao Xiaozhen Bao Xingtao Ren Yafeng Wang Yong Li Xiangyang Yu Haiyou Tan Xiaofeng Chen Zhuo song baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University Agricultural College Guizhou University

Tea gray blight disease is an important disease of the tea tree,which can harm tea seedlings,as well as the shoots,fresh leaves and mature leaves of trees for various growth *** disease can cause the tea plants weakened and severethe production loss of tea *** gray blight disease is one of the most important diseases in Guizhou tea *** this work,the pathogenic fungus was isolated,purified from the diseased tea leaves,and confirmed the pathogenicity of the pathogen according to Koch’s *** isolated strain of DX-1 was further identified by the morphology and molecular *** study indicated that DX-1 represents the features as following;on PDA medium,the colonyis obviously wheel round like,margin regular of the colonies;Reverse represents light yellow colony with white or colorless *** observation using the optical microscope and SEM indicated that theconidia represent fusi form with 4 diaphragms and 2 thin ends,and 2-3 appendages and1 appendage exist onthe top and the tail end of the cell,*** with（16.07±1.24） μm×（4.23±0.20） μm is colorless in the early stage,and the 3 septum near the center change brown in the late *** genes of EF-1α,β-tubulin and LSU were further amplified,purified and sequenced,and then the phylogenetic trees were constructed using MEGA6.0 software according to Neighbor-joining *** results indicated that DX-1 strain was closely related to Pestalotiopsis ***1（KJ623232.1） and YN1 B4（KU252565.1） against two phylogenetic treesof EF-1α and β-tubulin;and then clustered together with *** strain 3.919 2（JN940840.1） in a branch,and closely related to *** stain 3.909 8（JN940825.1） for the phylogenetic tree of LSU.

关键词： Tea gray blight disease Pathogenicity Pestalotiopsis theae

A pathogenic fungus Pestalotiopsis trachicarpicola was firstly identified from tea leaf spot disease in Yuqing county,Guizhou Province,China

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A pathogenic fungus Pestalotiopsis trachicarpicola was first...

中国植物病理学会2019年学术年会

作者： Yin Qiaoxiu Ren Yafeng Li Dongxue Wang Xue Wu Xian Jiang Shilong Wang Yong Wu Xian song baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease caused by pathogenic fungus seriously damaged the young leaves and shoots tea,and then affects the yield and quality of tea leaves in Yuqing county,Guizhou *** was an important disease of tea plant(Camellia sinensis) in this *** symptom of the disease represents the characteristic as follow:Purple-brown or reddish-brown dots appeared on leaves in the initial stage,and then the lesions developed into round or amorphous dark *** haloed yellow lesions were around the radial *** this study,we isolated and identified some fungi from tea leaves samples with the typical symptoms,and then conducted the pathogenicity test using Koch’s *** colonies of these isolates represent whitish to pale yellow,dense aerial mycelium,circular on PDA,with fimbriate edge,and produce black fruiting bodies,with developing in concentric *** colonies display yellow on reverse *** conidiomata was globose,black,semi-immersed on *** conidiogenous cells were fusiform,hyaline,short and thin *** consist of five cells [per size(17.49-30.16) μm×(4.74-7.4) μm].Basal cell represents conic to acute,hyaline,thin-walled,verruculose,2.66 to 7.70 μ*** apical cell represents conic to subcylindrical,hyaline,verruculose,2.99 to 6.69 μ*** middle three cells represent olive color,from the top the second cell was 3.52 to7.02 μm,the third cell was 3.37 to 6.82 μm,the fourth cell was 3.11 to 7.26 μm,two to three apical appendages,length of 6.69 to 14.85 μm,and a basal appendage,length of 2.66 to 7.70 μ*** primers ITS4/ITS5 for internal transcribed spacer(ITS) region,T1/BT2 B for β-tubulin gene(TUB),and EF1-728 F/EF-2 for translation elongation factor 1-alpha(TEF-1α) was used to amplify,the sequences were deposited in *** phylogenetic tree was constructed by PAUP v 4.0 b 10 software coupled with the Maximum Parsimony method,and the isolates were identical to reference strains Pestalotiopsis trachicarpicola MFLUCC 12-0266,and the

关键词： Pestalotiopsis trachicarpicola identification pathogenicity test leaf spot

Metabolome changes of tea leaf spot pathogen Phoma segeticola *** cultured on PDA with tea liquor

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Metabolome changes of tea leaf spot pathogen Phoma segeticol...

中国植物病理学会2019年学术年会

作者： Ren Yafeng Li Dongxue Wang Xue Yin Qiaoxiu Dharmasena Dissanayake-saman-pradeep Jiang Shilong Wu Xian song baoan Chen Zhuo Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University College of Agriculture Guizhou University

The fungal pathogen Phoma segeticola *** causes leaf spot on tea(Camellia sinensis) in Guizhou Province,*** study the mechanism of metabolomics during the stage of the pathogen infection,we design the experiment scheme of the pathogen culturing on PDA or PDA with tea liquor(Tea variety:Fudingdabaicha).The samples were detected using Nano-LC-MS-MS(TripleTOF 5600) under the mode of positive ion and negative ion,and then the data of the metabolites was further identified and quantified using the software XCMS and *** MS and MS-MS of the metabolites were annotated using the software metaX and in-house fragment spectrum *** were12 624 features in the positive mode,and 524 could be identified by ***,1 1008 features were detected in the negative mode,and 323 could be identified by *** addition,10850 high quality features were used to quantity following condition:ratio≥2 or ratio≤1/2;q value≤0.05;VIP≥1.122 2 and 1 048 were up-or down-regulated in the positive mode,*** the negative mode,9917 high quality features were used to quantity,and 1 192 and 1 078 features were up-or down-regulated,*** to the partial least-squares discrimination analysis,R value was 1.00,and Q2 value was 0.98 in the positive *** R value was 1.00,and Q2 value was 0.99 in the negative *** different metabolites were identified in 117 pathways,such as Biosynthesis of secondary metabolites(204),Biosynthesis of antibiotics(128),Microbial metabolism in diverse environments(95) and Arachidonic acid metabolism(45) et *** data would provide important reference for the pathogenesis of tea leaf spot.

关键词： Phoma segeticola var.camelliae Camellia sinensis metabolome

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新型光学活性α-氨基膦酸及其酯合成进展

有机化学 2003年第9期23卷 933-943页

作者：宋宝安吴扬兰何湘琼胡德禹刘刚杨松金林红蒋木庚贵州大学精细化工研究开发中心贵阳550025 南京农业大学理学院南京210095

α 氨基膦酸酯是一类很重要的天然氨基酸类似物 ,具有新颖的化学结构和多种生理活性 .综述了近年来光学活性α 氨基膦酸及酯的合成进展 .

关键词：光学活性 α-氨基膦酸 α-氨基膦酸酯合成立体化学化学结构

维普期刊数据库

基于课程模块化的教学改革探析——以高职院校工程经济学课改为例

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黑龙江科学 2021年第23期12卷 40-42页

作者：宋宝安南京城市职业学院南京211200

以高职院校工程经济学课改为例,对基于课程模块化的教学改革进行探析。从课程特点和教学中所遇到的问题出发,结合高职院校培养目标、课程标准以及模块化教学要求,以问题为导向,提出了课程改革的思路,对课程的模块化大体结构进行划分。... 详细信息

以高职院校工程经济学课改为例,对基于课程模块化的教学改革进行探析。从课程特点和教学中所遇到的问题出发,结合高职院校培养目标、课程标准以及模块化教学要求,以问题为导向,提出了课程改革的思路,对课程的模块化大体结构进行划分。在课程模块化的大前提下,提出了将传统教学方法与具体内容相结合的教学策略,概括成了“透”“明”“专”“精”“融”五字要诀,使大模块与模块内部教学方式、教学手段的小循环之间形成有机互动,相互补充。实践证明,课程模块化的教学改革取得了一定的积极效果,但也有一些问题值得思考和关注,这是未来需要进一步改进优化的地方。

关键词：课程模块化教学改革高职院校工程经济学课改

维普期刊数据库

Investigating the antifungal activity and mechanism of a microbial pesticide Ningnanmycin against Phoma segeticola ***

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Investigating the antifungal activity and mechanism of a mic...

中国植物病理学会2019年学术年会

Phoma segeticola *** was isolated from tea leaf spot disease in tea garden,Shiqian county,Guizhou *** leaf spot disease affected seriously the quality and yield of *** present,there are no effective measures of prevention and ***(NNM) is a new cytosine nucleoside peptide antibiotic with high efficiency and low *** method of mycelial growth rate was used to determine the antifungal activity of NNM against *** *** strain GZSQ-4,with EC of 1 287.54 U/*** order to further study the mechanism of NNM on GZSQ-4,the mycelium treated with NNM at the dosage of EC for 1 h and 14 h was observed by optical microscope,scanning electron microscopy and transmission electron microscopy,*** was found that the mycelium swelled,the mycelial cytoplasmic density decreased,the granular content appeared and the number of mitochondria granules *** GZSQ-4 being treated by NNM at the dosage of EC for 1 h,RNA-seq showed that 1 363 differentially expressed genes(DEGs) were identified with 743 DEGs upregulated and 620 DEGs *** GO enrichment analysis,the DEGs were most enriched in the structural constituent of ribosome of molecular function,ribosome of cellular component and the oxidation-reduction process of biological process including 56,38 and 116 DEGs,respectively(P<0.05).By KEGG enrichment analysis,the DEGs were most enriched in the pathway of ribosome including 58 *** selected randomly DEGs from treatment group of NNM for 1 h were verified by *** results showed that the expression level of DEGs was consistent with the result of *** results indicated that NNM may affect the processes of translation of *** study will provide useful information for management of tea leaf spot caused by *** *** and increase our understanding of action mechanism of NNM against the pathogen.

关键词： tea disease Phoma segeticola var.camellia Ningnanmycin antifungal activity action mechanism

超声辐射下O,O'-二烷基-α-取代苯基-α-苯磺酰氧基甲基膦酸酯的合成与生物活性

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有机化学 2013年第10期33卷 2226-2231页

作者：杨家强谷晴胡月维束波宋宝安刘静姿遵义医学院药学院遵义563003 遵义医学院生物化学与分子生物学教研室遵义563003 贵州大学精细化工研究中心贵阳550025

结合药物设计原理,对前期研究的活性结构类型进行优化.在超声辐射下,以α-羟基膦酸酯、苯磺酰氯为原料设计合成系列新型O,O'-二烷基-α-取代苯基-α-苯磺酰氧基甲基膦酸酯衍生物,考察不同溶剂、反应温度、反应时间、反应功率对产率的影... 详细信息

结合药物设计原理,对前期研究的活性结构类型进行优化.在超声辐射下,以α-羟基膦酸酯、苯磺酰氯为原料设计合成系列新型O,O'-二烷基-α-取代苯基-α-苯磺酰氧基甲基膦酸酯衍生物,考察不同溶剂、反应温度、反应时间、反应功率对产率的影响,得到最佳反应条件.该方法简便可行、时间较短、产率较高.目标物结构经1H NMR,IR,MS及元素分析确认,并研究了质谱裂解规律.初步生物活性测试表明:部分目标物对肿瘤细胞A-549有较好的抑制作用.

关键词： α-羟基膦酸酯磺酸酯超声合成抗肿瘤活性

维普期刊数据库

苯胺—三唑基—甲烷新化合物合成与构效关系的研究

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贵州科学 1987年第1期 59-66页

作者：宋宝安贵州大学

三唑酮是苯氧基——三唑基——甲烷类的化合物,它是酵母和真菌的麦角甾醇生物合成的一种强抑制剂。我们设计并合成了它们的生物电子等排体——苯胺——三唑——甲烷。这些化合物培养皿试验表明:三个新化合物对棉花枯萎病菌、棉花红腐病... 详细信息

三唑酮是苯氧基——三唑基——甲烷类的化合物,它是酵母和真菌的麦角甾醇生物合成的一种强抑制剂。我们设计并合成了它们的生物电子等排体——苯胺——三唑——甲烷。这些化合物培养皿试验表明:三个新化合物对棉花枯萎病菌、棉花红腐病菌、水稻纹枯病菌等有抑菌活性,用CNDO法分析了这些化合物结构与抑菌活性的关系。

关键词：化合物合成三唑构效关系苯胺基生物合成棉花红腐病水稻纹枯病菌棉花枯萎病菌苯氧基麦角甾醇