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Insect Science 2011年第1期18卷 67-77页

作者： Peng Wang Di-Bing Sun bao-li qiu Shu-Sheng liu Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences Zhejiang University Hangzhou Department of Entomology South China Agricultural University Guangzhou China

Recent phylogenetic analysis using mitochondrial cytochrome oxidase I （mtCOI） sequences ofBemisia tabaci worldwide indicates that the whitefly comprises at least 24 morphologically indistinguishable but genetically distinct cryptic species. While evidence of reproductive isolation has been reported for some of the putative species, more extensive crossing experiments are required to clarify the systematics of this species com- plex. In this study, we established laboratory cultures for six putative species ofB. tabaci collected in China. We conducted 22 inter-species crosses among the six putative species. The data and those reported previously were collated, and the combined dataset covered all the 30 possible inter-species crosses among the six putative species. Intra-species controls always produced female and male progeny and the proportions of females in the first gen- eration （F1） ranged from 56% to 70%. However, in inter-species crosses female progeny were rarely produced, and the few F1 females produced in four of the 30 inter-species crosses were either sterile or significantly weaker in viability. These results demonstrate a pattern of complete reproductive isolation among the six putative species and show that they are six cryptic species in the B. tabaci complex.

关键词： Bemisia tabaci biological invasion genetic diversity reproductive incompatibility systematics

Genetic mutations associated with chemical resistance in the cytochrome P450 genes of invasive and native Bemisia tabaci （Hemiptera： Aleyrodidae） populations in China

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Insect Science 2009年第3期16卷 237-245页

作者： bao-li qiu li liu Xiao-Xi li Vartika Mathur Zhen-Qiang Qin Shun-Xiang Ren Department of Entomology South China Agricultural University Guangzhou China Department of Zoology Sri Venkateswara CollegeUniversity of Delhi Delhi 110021 India

Bemisia tabaci （Gennadius） is a species complex, and its two most damaging biotypes B and Q are globally distributed pests. Despite increasing biological and economic impacts, little is known about the evolutionary mechanisms that favor their competition with native populations. Here, we investigated the genetic mutations in the P450 gene of the invasive B, Q biotypes and the native Cv population. Four mutations associated with chemical resistance, Pro-Leu, Ala-Ser, Ser-Phe and Trp-Leu, were found in the cytochrome P450 CYP6C and CYP9F genes of the B and Q biotypes. Bioassay results also revealed that both the B and Q biotypes have about 12-47 times more resistance to acephate, beta- cypermethrin, methomyl, and 5-7 times more resistance to imidacloprid insecticide than Cv population. Our results provide a molecular approach for better understanding and monitoring the pesticide resistances of invasive and native B. tabaei populations in China.

关键词： Bemisia tabaci biotype cytochrome P450 gene genetic mutation pesticide resistance

Delayed development of the whitefly （Bemisia tabaci） and increased parasitism by Encarsia bimaculata in response to sublethal doses of piperonyl butoxide

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Insect Science 2012年第3期19卷 403-411页

作者： Ming-Hui Qian Qiong-Bo Hu Shun-Xiang Ren Nasser S.Mandour bao-li qiu P.A.Stansly Department of Forest Protection Guangdong Academy of Forestry College of Natural Resources and Environment South China Agricultural UniversityGuangzhouChina Department of Plant Protection Faculty of AgricultureSuez Canal UniversityIsmailiaEgypt University of Florida-IFAS ImmokaleeFloridaUSA

Effects of sublethal piperonyl butoxide （PB） on parasitization of Bemisia tabaci （Gennadius）（Hemiptera： Aleyrodidae） by Encarsia bimaculata Heraty et Polaszek （Hymenoptera： Aphelinidae） were evaluated both in cage and greenhouse experiments. When first, second and third instar B. tabaci nymphs were treated with PB, all but the first instar were significantly prolonged. Data indicated that sublethal PB could improve E. bimaculata parasitism rates without influencing parasitoid eclosion rates. Prolonged development increased rates of parasitism by E. bimaculata, from 17.6% to 24.7% in cages, presumably by increasing the duration of host exposure. Sublethal PB combined with E. bimaculata as an integrated approach to control B. tabaci was evaluated using life table parameters under greenhouse conditions. Indices of population trend （I） calculated from life tables were estimated at 4.6 for B. tabaci exposed to PB and parasitoids compared to 14.1 with parasitoids alone and 23.5 in un- treated controls. The results showed that after PB was sprayed and parasitoids introduced, development ofB. tabaci was delayed and the peak of each stage was postponed. The older nymphal stage had highest mortality, primarily due to mortality caused by parasitism by E. bimaculata.

关键词： Bemisia tabaci Encarsia bimaculata parasitization sublethal piperonyl butoxide

Silencing of V-ATPase-E gene causes midgut apoptosis of Diaphorina citri and affects its acquisition of Huanglongbing pathogen

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Insect Science 2023年第4期30卷 1022-1034页

作者： Chang-Fei Guo Jun-Hong qiu Yu-Wei Hu Pei-Ping Xu Ying-Qi Deng ling Tian Yi-Yun Wei Wen Sang Yu-Tao liu bao-li qiu Engineering Research Center of Biocontrol Ministry of Education and Guangdong ProvinceSouth China Agricultural UniversityGuangzhouChina Guangdong Laboratory for lingnan Modern Agriculture GuangzhouChina Chongqing Key Laboratory of Vector Insects College of Life SciencesChongqing Normal UniversityChongqingChina Key Laboratory of South China Modern Biological Seed Industry MARANational S&T Innovation Center for Modern Agricultural IndustryGuangzhouChina College of Animal Science South China Agricultural UniversityGuangzhouChina

The Asian citrus psyllid,Diaphorina citri Kuwayama,is among the most im-portant pests of *** is the main vector of the Huanglongbing(HLB)pathogen Can-didatus liberibacter asiaticus(CLas),which causes severe losses in citrus *** of *** is therefore of paramount importance to reduce the spread of *** this re-gard,using RNA interference(RNAi)to silence target genes is a useful strategy to control *** this study,using RNAi,we examined the biological functions of the V-ATPase subunit E(V-ATP-E)gene of ***,including its effect on acquisition of *** amino acid sequence of V-ATP-E from *** had high homology with proteins from other insects.V-ATP-E was expressed at all *** life stages analyzed,and the expres-sion level in mature adults was higher than that of teneral *** of V-ATP-E resulted in a significant increase in mortality,reduced body weight,and induced cell apop-tosis of the *** *** reduced expression of V-ATP-E was indicated to inhibit CLas passing through the midgut and into the hemolymph,leading to a majority of CLas being confined to the *** addition,double-stranded RNA of *** V-ATP-E was safe to non-target parasitic *** results suggest that V-ATP-E is an effective RNAi target that can be used in *** control to block CLas infection.

关键词： apoptosis CLas infection Diaphorina citri RNA interference V-ATPase subunit E

Antagonistic interaction between male-killing and cytoplasmic incompatibility induced by Cardinium and Wolbachia in the whitefly,Bemisia tabaci

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Insect Science 2021年第2期28卷 330-346页

作者： Ning Lv Jing Peng Xin-Yi Chen Chang-Fei Guo Wen Sang Xing-Min Wang Muhammad ZAhmed Yong-Yu Xu bao-li qiu Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province South China Agricultural UniversityGuangzhouChina Engineering Research Center of Biocontrol Ministry of EducationGuangzhouChina Maoming Branch Guangdong Laboratory of Lingnan Modern AgricultureMaomingGuangdong ProvinceChina Florida Department of Agriculture and Consumer Services 1911 SW 34th StreetGainesvilleUSA College of Plant Protection Shandong Agricultural UniversityTaianShandong ProvinceChina

Cardinium and Wolbachia are maternally inherited bacterial symbionts of arthropods that can manipulate host reproduction by increasing the fitness of infected ***,we report that Cardinium and Wolbachia coinfection induced male-killing and cytoplasmic incompatibility(CI)when they coexisted in a cryptic species of whitefly,Bemisia tabaci Asia *** and Wolbachia symbionts were either singly or simul-taneously localized in the bacteriocytes placed in the abdomen of *** nymphs and ***-Wolbachia coinfection induced male-killing and resulted in a higher female sex ratio in the intraspecific amphigenetic progeny of Asia II7 IcwH and IcwL lines;interestingly,male-illing induction was enhanced with increased Cardinium ***,single infection of Wolbachia induced partial CI in the Asia II7 Iw line and resulted in reduced fecundity,higher embryonic mortality,and lower female sex *** uninfected Asia II7 Iu line had significantly higher fecundity,lower embryonic and nymphal mortalities,and a lower level of CI than both the Wolbachia infected Asia II7 Iw line and the Cardinium--Wolbachia-coinfected Asia II7 IcwH *** findings indicate that Cardinium-Wolbachia coinfection induced male-killing,which may have had antag-onistic effects on Wolbachia-induced CI in the Asia II7 *** the first time,our study revealed that *** Asia II7 reproduction is co-manipulated by Cardinium and Wolbachia endosymbionts.

关键词： antagonistic interaction Bemisia tabaci coinfection endosymbiont repro-ductive manipulation sex ratio

Host plants and natural enemies of Bemisia tabaci （Hemiptera： Aleyrodidae）in China

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Insect Science 2011年第1期18卷 101-120页

作者： Shao-Jian li Xia Xue Muhammad Z. Ahmed Shun-Xiang Ren Yu-Zhou Du Jian-Hui Wu Andrew G. S. Cuthbertson bao-li qiu Department of Entomology South China Agricultural University Guangzhou School of Horticulture and Plant Protection Yangzhou University Yangzhou Jiangsu Province China The Food and Environment Research Agency Sand Hutton York UK

The sweetpotato whitefly, Bemisia tabaci, has been a destructive pest in China for over the past two decades. It is an extremely polyphagous insect, being recorded feeding on hundreds of host plants around the world. Potential host plants and natural enemies of B. tabaci in the south, southeast, middle, north and northwest of China were investigated during the last decade. In total 361 plant species from 89 families were recorded in our surveys. Plants in the families Compositae, Cruciferae, Cucurbitaceae, Solanaceae and Leguminosae were the preferred host species for B. tabaci, which therefore suffered much damage from this devastating pest due to their high populations. In total, 56 species of parasitoids, 54 species of arthropod predators and seven species of entomopathogenic fungi were recorded in our surveys. Aphelinid parasitoids from Encarsia and Eretrnocerus genera, lady beetles and lacewings in Coleoptera and Neuroptera were found to be the dominant arthropod predators of B. tabaci in China. The varieties of host plant, their distribution and the dominant species of natural enemies of B. tabaci in different regions of China are discussed.

关键词： Bemisia tabaci China host plant natural enemy survey

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论王国维、蔡元培美育思想中的审美与功利性

扬州大学学报（人文社会科学版） 2007年第3期11卷 47-50页

作者：包莉秋扬州大学文学院江苏扬州225002

在中国,美育思想古已有之,但作为学术话语却是在近代由王国维从西方引入,蔡元培等继而进一步阐释的。在他们看来,美育具有双重功用,即集超功利(即审美)与功利于一体:前者认为美育的目的是使人放弃欲望的纠缠而获得纯粹的快乐,令人愉悦,... 详细信息

在中国,美育思想古已有之,但作为学术话语却是在近代由王国维从西方引入,蔡元培等继而进一步阐释的。在他们看来,美育具有双重功用,即集超功利(即审美)与功利于一体:前者认为美育的目的是使人放弃欲望的纠缠而获得纯粹的快乐,令人愉悦,归根结底是一种情感教育;后者以为美育的目的在于辅助德育和智育,从而帮助教育塑造完美人格。

关键词：美育审美功利王国维蔡元培

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基于大数据的园林割草机器人轨迹规划仿真

计算机仿真 2020年第8期37卷 261-265页

作者：宝秋利梁显丽内蒙古农业大学内蒙古包头014109

传统割草机器人轨迹规划未能规划出最佳轨迹,导致规划能力较低,园林实施全方位割草作业效率降低。提出基于大数据的园林割草机器人轨迹规划方法。将CCD摄像头安装于割草机器人,检测草坪区域,分辨出草坪与非草坪区域;复杂环境下通过构建... 详细信息

传统割草机器人轨迹规划未能规划出最佳轨迹,导致规划能力较低,园林实施全方位割草作业效率降低。提出基于大数据的园林割草机器人轨迹规划方法。将CCD摄像头安装于割草机器人,检测草坪区域,分辨出草坪与非草坪区域;复杂环境下通过构建机器人运动模型,操控割草机器人,通过FPGA与神经网络相结合,调整权值来定位与导航机器人运动路线,规划出割草机器人运行的最佳轨迹;通过实验证明,所提方法可以准确穿越障碍物,具有精准的运动轨迹,并且适用于任何复杂园林环境,为城市园林绿化提供了更加便捷的方式。

关键词：大数据割草机器人神经网络轨迹规划运动模型

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论消费文化背景下文学研究的与时俱进

涪陵师范学院学报 2006年第5期22卷 9-13,24页

作者：包莉秋扬州大学文学院江苏扬州225002

消费文化下的文学研究是当前文艺学的热点之一。消费文化是在消费成为一种主流时,社会主流群体迫不得已而为之的一种集体意识的能动反映,具有多元性、包容性和开放性,在此情景下的文学研究所面临的是后现代语境和文学上启蒙范式的失落... 详细信息

消费文化下的文学研究是当前文艺学的热点之一。消费文化是在消费成为一种主流时,社会主流群体迫不得已而为之的一种集体意识的能动反映,具有多元性、包容性和开放性,在此情景下的文学研究所面临的是后现代语境和文学上启蒙范式的失落。消费文化对文学研究的激荡与提升可使其更加关注视觉文化与文学接受者的地位,并且更加关注自身的理论建设。当然,文学研究还需对自身进行及时重建与调整,体现与时俱进的时代特征。

关键词：消费文化文化研究文学研究文学启蒙范式

从1895-1916年小说报刊的办刊宗旨看近代小说的功用观

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西华师范大学学报（哲学社会科学版） 2010年第3期 15-20页

作者：包莉秋长江师范学院文学与新闻学院重庆408100

小说因"小说界革命"一改其在文体中的地位,小说报刊随之纷纷创刊。综观1895-1916年小说报刊的发刊词,其宗旨可分为三类:一是承认小说具有巨大的社会功用,是唤醒国民、开通民智的重要工具;二是以为小说具有审美性,是文学倾向于美的一种;... 详细信息

小说因"小说界革命"一改其在文体中的地位,小说报刊随之纷纷创刊。综观1895-1916年小说报刊的发刊词,其宗旨可分为三类:一是承认小说具有巨大的社会功用,是唤醒国民、开通民智的重要工具;二是以为小说具有审美性,是文学倾向于美的一种;三是主张小说有消遣、娱乐的功能。特殊的时代背景决定了小说在当时主要是为政治、社会服务的,而小说的审美性、娱乐性则丰富了小说的功用。

关键词：小说功利审美娱乐