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Journal of Ocean University of China 2015年第4期14卷 685-690页

作者： LIAN Shanshan HE Yan LI Xue ZHAO Bosong hou Rui HU Xiaoli ZHANG Lingling bao Zhenmin Key Laboratory of Marine Genetics and Breeding(MGB) of Ministry of Education College of Marine Life Sciences Ocean University of China

DNA methylation is an important epigenetic regulatory mechanism that influences genomic stability, gene activation, X-chromosome inactivation and other factors. A change in DNA methylation is usually associated with aging and cellular senescence. DNA methyltransferase 1(DNMT1) is the most abundant DNA methyltransferase, and it plays an important role in maintaining the established methylation pattern during DNA replication in vertebrates. Although the effect of aging on DNA methylation has been well studied in vertebrates, little research has been conducted in invertebrates, especially in marine bivalves. In this study, we examined global DNA methylation levels in four groups of adult Zhikong scallop Chlamys farreri at different ages. The results showed that both the age and tissue type had a strong effect on the DNA methylation. In addition, a significant decrease in DNA methylation with aging(1–4 years) can be detected in mantle, kidney and hepatopancreas. We further measured the change in DNMT1 transcript abundance using quantitative reverse transcription PCR(q RT-PCR), which revealed that DNMT1 transcription significantly decreased with aging in mantle and hepatopancreas and strongly correlated with DNA methylation(R = 0.72). Our data provided greater insight into the aging-related decline of DNA methylation, which could aid in gaining a better understanding of the relationship between DNA methylation and the aging process in bivalve mollusks.

关键词： aging DNA methylation DNMT1 transcription Chlamysfarreri

DNP NMR reveals the hidden surface C–C bond growth mechanism over ZnAlO_(x) during syngas conversion

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Journal of Energy Chemistry 2022年第4期31卷 640-644页

作者： Pan Gao Dong Xiao Zhenchao Zhao Subhradip Paul Frédéric Blanc Xiuwen Han Guangjin hou Xinhe bao State Key Laboratory of Catalysis National Laboratory for Clean Energy2011-Collaborative Innovation Center of Chemistry for Energy MaterialsDalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian 116023LiaoningChina DNP MAS NMR Facility Sir Peter Mansfield Imaging CentreUniversity of NottinghamNottingham NG72RDUnited Kingdom Department of Chemistry University of LiverpoolCrown StreetLiverpool L697ZDUnited Kingdom Stephenson Institute for Renewable Energy University of LiverpoolCrown StreetLiverpool L697ZDUnited Kingdom

Direct catalytic transformation of syngas(CO/H_(2)) into high value-added hydrocarbons has been one of the most attractive,yet challenging issues in C1 chemistry. In recent years, the concept of oxide-zeolite(OXZEO) bifunctional catalysis has emerged and spurred renewed enthusiasm in this field [1,2]. This method describes the use of selected oxide-zeolite-based composite catalysts for the direct syngas-to-hydrocarbons conversion.

关键词： zeolite hydrocarbons syngas

Biodegradation Kinetics and Mechanism of a Typical Coal Gasification Pollutant:Quinoline

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Journal of Harbin Institute of Technology(New Series) 2014年第6期21卷 121-128页

作者： Peng Xu Hong-Jun Han Sheng-Yong Jia bao-Lin hou Hai-Feng Zhuang Qian Zhao State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

A strain Pseudomonas ***1 was isolated from activated sludge and characterized by morphological observation,biochemical and physiological identification as well as 16 S rRNA gene sequence *** was a gram-negative,non-motile,rod-shaped with colonies which were white,opaque,small and wrinkled on solid *** strain was negative for catalase,gelatin liquefaction,M.R and V.P tests and was unable to reduce / restore *** 1406 bp 16 S rRNA gene fragment of PP1 was more than 99% identical to the Pseudomonas *** of quinoline as single substrate was conducted in batch experiments at different temperatures( 15- 40 ℃) and p H values( 5- 10).The results indicate that the optimum conditions for the degradation of quinoline by PP1 were 30 ℃ and p H *** ***1 was able to degrade 97.1%,95.4%,94.8%,63.6% and 40.4% quinoline when initial concentrations of quinoline were 50,100,200,300,and 400 mg / L,respectively,and lag phases were prolonged from 2 h to 10 *** maximum degradation rate( q) was obtained at an initial quinoline concentration( S0) of about 100 mg/L with q of *** experimentally obtained q values at various initial S0 were fitted by Haldane model,Yano model,Aiba model,Edward model and Webb model,and the results demonstrated that Haldane model gives the best fit for strain PP1 with coefficient of determination,R2= 0.9124 and SDavg= *** metabolic intermediates were identified by high performance liquid chromatograph( HPLC) and GC / ***,a possible pathway containing 5,6-dihydroxy-2-oxo-1,2-dihydroquinoline as an intermediate was proposed for the first time.

关键词： pseudomonas sp. quinoline biodegradation kinetics pathway

Origin of Negative Imaginary Part of Effective Permittivity of Passive Materials

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Chinese Physics Letters 2017年第9期34卷 105-108页

作者： Kai-Lun Zhang Zhi-Ling hou Ling-bao Kong Hui-Min Fang Ke-Tao Zhan School of Science ＆ Beijing Laboratory of Enyironmentally Harmful Chemicals Assessment Beijing University of Chemical Technology Beijing 100029 Key of Science Beijing Technology and Business University Beijing 100048

The anti-resonant phenomenon of effective electromagnetic parameters of metamaterials has aroused controversy due to negative imaginary permittivity or permeability. It is experimentally found that the negative imaginary permittivity can occur for the natural passive materials near the Fabry Perot resonances. We reveal the nature of negative imaginary permittivity, which is correlated with the magnetoelectric coupling. The anti-resonance of permittivity is a non-inherent feature for passive materials, while it can be inherent for devices or metamaterials. Our finding validates that the negative imaginary part of effective permittivity does not contradict the second law of thermodynamics for metamaterials owing to the magnetoelectric coupling.

关键词： Origin of Negative Imaginary Part of Effective Permittivity of Passive Materials FPR

Identification of microRNA-like RNAs in Ophiocordyceps sinensis

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Science China(Life Sciences) 2019年第3期62卷 349-356页

作者： Wen Zhang Xiaona Li Lina Ma Uzair Urrehman Xilinqiqige bao Yujing Zhang Chen-Yu Zhang Dongxia hou Zhen Zhou State Key Laboratory of Pharmaceutical Biotechnology Jiangsu Engineering Research Center for microRNA Biology and Biotechnology NJU Advanced Institute for Life Sciences (NAILS) School of Life Sciences Nanjing University The Drug Laboratory of Mongolian Medicine and Pharmacy Institute Inner Mongolia International Mongolian Hospital

Ophiocordyceps sinensis is well known as a traditional Chinese medicine and has widely been used for over 2,000 years to stimulate immune system, decrease blood pressure and to inhibit tumor growth. While miRNAs are increasingly recognized for their roles in post-transcriptional regulation of gene expression in animals and plants, miRNAs in fungi were less studied until the discovery of microRNA-like RNA(milRNA). High-throughput sequencing and bioinformatics approaches were used to identify conserved and novel milRNAs in O. sinensis. 40 conserved milRNAs were identified, while 23 pre-miRNA candidates encoding 31 novel milRNAs were predicted. Furthermore, the potential target genes of milRNAs in human were predicted and gene ontology analysis was applied to these genes. Enrichment analysis of GO-represented biological process showed that target genes of both conserved and novel milRNAs are involved in development, metabolic and immune processes, indicating the potential roles of milRNAs of O. sinensis in pharmacological effects as health food and traditional Chinese medicine. This study is the first report on genome-wide analysis of milRNAs in O. sinensis and it provides a useful resource to further study the potential roles of milRNAs as active components of O. sinensis in health food or traditional Chinese medicine.

关键词： Ophiocordyceps sinensis O.sinensis miRNA milRNA high-throughput sequencing

Improving the energy efficiency of surface dielectric barrier discharge devices for plasma nitric oxide conversion utilizing active flow control

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Chinese Journal of Chemical Engineering 2023年第1期53卷 270-279页

作者： An Wang Zhongyu hou National Key Laboratory of Science and Technology on Micro/Nano Fabrication Shanghai Jiao Tong UniversityShanghai 200240China Department of Micro/Nano Electronics School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Improving energy efficiency in plasma NO removal is a critical *** the surface dielectric barrier discharge(SDBD)device is considered as a combination of multiple plasma actuators,the induced plasma aerodynamic effect cannot be ignored,which can affect the mass transfer,then affect the chemical *** SDBD devices with different electrode arrangements are studied for NO *** correspond to different flow *** find that the energy efficiency in an SDBD device with a common structure(Type 1)is 28%lower than that in SDBD devices with a special arrangement(Types 2–5).Two reasons may explain the ***,fewer active species are produced in Type 1 because the development of discharge is hindered by the mutually exclusive electric field forces caused by the symmetrically distributed charged ***,the plasma wind induced by the plasma actuator can enhance the mass and heat *** mixing of reactants and products is better in Types 2–5 than Type 1 due to higher turbulence kinetic energy.

关键词： Flue gas Radical Oxidation Surface dielectric barrier discharge(SDBD) Plasma aerodynamic effect Plasma NO conversion

Evidence for the role of KIF17 in fish spermatid reshaping:expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

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Journal of Oceanology and Limnology 2021年第6期39卷 2322-2335页

作者： Jingqian WANG Xinming GAO Xuebin ZHENG Chen DU Congcong hou Qingping XIE bao LOU Feng LIU Shan JIN Junquan ZHU Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Zhejiang Academy of Agricultural Sciences Hangzhou 310021China

The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in ***,its role in fish spermiogenesis has not been ***,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in Larimichthys polyactis *** full-length cDNA of *** kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino *** analyses showed that *** KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail *** Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the *** in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this *** immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during *** findings suggested that KIF17 is involved in *** *** particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid *** addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in ***.

关键词： KIF17 spermiogenesis Larimichthys polyactis spermatid reshaping

Environmentally friendly expanded graphite-doped ZnO superhydrophobic coating with good corrosion resistance in marine environment

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Rare Metals 2023年第9期42卷 3075-3087页

作者： Si-Wei Wu Quan-Tong Jiang Shuai Yuan Qing-Kun Zhao Chang Liu Heng Tang Qiang Sun Ji-Zhou Duan bao-Rong hou Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou510301China CAS Key Laboratory of Marine Environmental Corrosion and Bio-Fouling Institute of OceanologyChinese Academy of SciencesQingdao266071China Sanya Institute of Ocean Eco-Environmental Engineering Sanya572000China University of Chinese Academy of Sciences Beijing100049China Open Studio for Marine Corrosion and Protection Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266071China

Designing and fabricating the marine anti-corrosion materials without fluorine by superhydrophobic method is a huge challenge. In this study, an environmentally friendly composite coating was prepared by combing modified expanded graphite(EAG) with nano zinc oxide(ZnO). This coating showed superhydrophobic surface and good corrosion resistance. Fourier transform infrared spectroscopy(FITR), X-ray diffraction(XRD),and scanning electron microscopy(SEM) were used to characterize the materials in fabricating process of the coating. The properties of three composite coatings(ZnO,EAG, and EAG@ZnO) were analyzed, including surface roughness, water contact angle, corrosion resistance, selfcleaning and anti-fouling. The combination of ZnO and EAG caused a big water contact angle, leading superhydrophobic surface of the composite coatings. The electrochemical results showed that the superhydrophobic EAG@ZnO coating had a larger capacitive arc diameter and charge transfer resistance, indicating the enhanced anti-corrosion resistance. Meanwhile, the EAG@ZnO coating also showed good self-cleaning and anti-fouling performance according to solid and liquid pollutants *** addition, the mechanical properties and stability of the superhydrophobic EAG@ZnO coatings were evaluated by knife peeling and finger scratch tests. In summary, these superhydrophobic and anti-fouling EAG@ZnO composite coatings provide a potential application in marine corrosion and protection field.

关键词： Coating Superhydrophobic Anti-corrosion Anti-fouling Environmentally friendly

Effects and Mechanism of Action of Artemisinin on Mitochondria of Plasmodium berghei

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Chinese Journal of Integrative Medicine 2020年第4期26卷 277-282页

作者： hou Hong-ping ZHANG Guang-ping MA Li-na SU Ping ZHANG Zhong-xiu DAI bao-qiang YE Zu-guang Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing(100700)China

Objective:To study the antimalarial effects and mechanisms of artemisinin(Qinghaosu in Chinese,QHS)on mitochondria in mice infected with Plasmodium ***:A total of 108 C57 mice infected with Plasmodium berghei were randomly divided into 3 groups by weight:the control group,200 and 400 mg/kg QHS *** two QHS treatment groups were further divided into 4 sub-groups with 12 animals each time according to the treatment time,0.5,1,2,and 4 *** saline was intragastrically(i.g.)administered to the control *** other two groups received different doses of QHS by *** were treated once with QHS for different detection time as follows:0.5,1,2,and 4 *** mitochondrial energy metabolism,oxidative damage,membrane potential,and membrane permeability and other indexes were ***:After administration of 200 and 400 mg/kg QHS,adenosine triphosphate(ATP)levels in Plasmodium and its mitochondria were reduced(P<0.05),the levels of reactive oxygen species(ROS)and malondialdehyde(MDA)were in creased(P<0.05),and the activity of superoxide dismutase(SOD)was also increased(P<0.05).At the same time,the membrane potential of the mitochondria was reduced and the degree to which the membrane permeability transition pore was ope ned was irreversibly in creased(P<0.05).Conclusions:Mitoch on dria in Plasmodium were the targets of QHS,which can adversely affect mitochondrial energy metabolism,oxidative damage,membrane potential,and membrane opening,and ultimately exert an antimalarial effect.

关键词： Qinghaosu Clinese medicine Plasmodium mitochondria antimalarial effect

Unveiling the promotion role of ZnO on Zn-Al spinel oxide for CO2 hydrogenation

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Journal of Energy Chemistry 2025年第1期 18-25页

作者： Tongyao Wang Xinlong Yao Lixin Liang Hongyu Chen Pan Gao Guangjin hou State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

The Zn-Al spinel oxide stands out as one of the most active catalysts for high-temperature methanol synthesis from CO2hydrogenation. However, the structure–activity relationship of the reaction remains poorly understood due to challenges in atomic-level structural characterizations and analysis of reaction intermediates. In this study, we prepared two Zn-Al spinel oxide catalysts via coprecipitation（ZnAl-C）and hydrothermal（ZnAl-H） methods, and conducted a comparative investigation in the CO2hydrogenation reaction. Surprisingly, under similar conditions, ZnAl-C exhibited significantly higher selectivity towards methanol and DME compared to ZnAl-H. Comprehensive characterizations using X-ray diffraction（XRD）, Raman spectroscopy and electron paramagnetic resonance（EPR） unveiled that ZnAl-C catalyst had abundant ZnO species on its surface, and the interaction between the ZnO species and its ZnAl spinel oxide matrix led to the formation of oxygen vacancies, which are crucial for CO2adsorption and activation. Additionally, state-of-the-art solid-state nuclear magnetic resonance（NMR） techniques,including ex-situ and in-situ NMR analyses, confirmed that the surface ZnO facilitates the formation of unique highly reactive interfacial formate species, which was readily hydrogenated to methanol and DME. These insights elucidate the promotion effects of ZnO on the ZnAl spinel oxide in regulating active sites and reactive intermediates for CO2-to-methanol hydrogenation reaction, which is further evidenced by the significant enhancement in methanol and DME selectivity observed upon loading ZnO onto the ZnAl-H catalyst. These molecular-level mechanism understandings reinforce the idea of optimizing the ZnO-ZnAl interface through tailored synthesis methods to achieve activity-selectivity balance.

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