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A modified laser-induced choroidal neovascularization animal model with intravitreal oxidized low-density lipoprotein

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International Journal of Ophthalmology(English edition) 2020年第8期13卷 1187-1194页

作者： Tong Wu Kuan-Rong Dang Ya-Fen Wang bao-zhen lyu Wen-Qin Xu Guo-Rui Dou Jian Zhou Yan-Nian Hui Hong-Jun Du Department of Ophthalmology Xijing HospitalEye Institute of Chinese PLAFourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Anatomy and Histology and Embryology Xi’an Health SchoolXi’an 710032Shaanxi ProvinceChina The Orbital Disease Institute of the Third Medical Center of PLA General Hospital Beijing100039China

AIM: To investigate whether intravitreal injection of oxidized low-density lipoprotein(OxLDL) can promote laserinduced choroidal neovascularization(CNV) formation in mice and the mechanism involved, thereby to develop a better animal ***: C57BL6/J mice were randomized into three groups. Immediately after CNV induction with 532 nm laser photocoagulation, 1.0 μL of OxLDL [100 μg/m L in phosphate-buffered saline(PBS)] was intravitreally injected, whereas PBS and the same volume low-density lipoprotein(LDL;100 μg/m L in PBS) were injected into the vitreous as controls. Angiogenic and inflammatory cytokines were measured by quantitative real-time polymerase chain reaction(q RT-PCR) and Western blotting(WB) after 5 d, and CNV severity was analyzed by choroid flat mount and immunofluorescence staining after 1wk. In vitro, retinal pigment epithelial(RPE) cell line(ARPE19) were treated with OxLDL(LDL as control) for 8 h. Angiogenic and inflammatory cytokine levels were measured. A specific inhibitor of lectin-like oxidized low-density lipoprotein receptor 1(LOX1) was used to evaluate the role of LOX1 in this ***: At 7 d after intravitreal injection of 1 μL(100 μg/mL) OxLDL, T15-labeled OxLDL was mainly deposited around the CNV area, and the F4/80-labeled macrophages, the CD31-labeled vascular endothelial cells number and CNV area were increased. Meanwhile, WB and qR T-PCR results showed that vascular endothelial growth factor(VEGF), CC chemokine receptor 2(CCR2), interleukin-6(IL-6), IL-1β, and matrix metalloproteinase 9(MMP9) expressions were increased, which was supported by in vitro experiments in RPE cells. LOX1 inhibitors significantly reduced expressions of inflammatory factors IL-1β and VEGF. CONCLUSION: A modified laser-induced CNV animal model is established with intravitreal injection of 1 μL(100 μg/mL) of OxLDL at 7 d, which at least partially through LOX1. This animal model can be used as a simple model for studying the role of OxLDL in age-

关键词： age-related macular degeneration choroidal neovascularization oxidized low-density lipoprotein animal model

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Compound heterozygous mutation of AFG3L2 causes autosomal recessive spinocerebellar ataxia through mitochondrial impairment and MICU1 mediated Ca2+overload

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Science China(Life Sciences) 2025年第2期68卷 484-501页

作者： Hongyu Li Qingwen Ma Yan Xue Linlin Cai Liwen bao Lei Hong Yitao Zeng Shu-zhen Huang Richard H.Finnell Fanyi Zeng Shanghai Institute of Medical Genetics Shanghai Children’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200040China Department of Histo-Embryology Genetics and Developmental BiologyShanghai Jiao Tong University School of MedicineShanghai 200025China NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology Shanghai Key Laboratory of Embryo and Reproduction EngineeringShanghai 200040China Center for Precision Environmental Health Baylor College of MedicineHouston 77030USA School of Pharmacy Macao University of Science and TechnologyMacao 999078China

Autosomal recessive spinocerebellar ataxias(SCARs)are one of the most common neurodegenerative diseases characterized by progressive *** SCARs are known to be caused by mutations in multiple genes,there are still many cases that go undiagnosed or are *** this study,we presented a SCAR patient,and identified a probable novel pathogenic mutation(c.1A>G,p.M1V)in the AFG3L2 start *** proband's genotype included heterozygous mutations of the compound AFG3L2(p.[M1V];[R632X](c.[1A>G];[1894.C>T])),which were inherited from the father(c.1A>G,p.M1V)and mother(c.1894C>T,p.R632X).Functional studies performed on hi PSCs(human induced pluripotent stem cells)generated from the patients and HEK293T cells showed that the mutations impair mitochondrial function and the unbalanced expression of AFG3L2 mRNA and protein ***,this novel mutation resulted in the degradation of the protein and the reduction of the stability of the AFG3L2 protein,and MCU(mitochondrial calcium uniporter)complex mediated Ca2+overload.

关键词： AFG3L2 gene autosomal recessive spinocerebellar ataxia mitochondrial impairment Ca2+overload

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Study on the Radiation Synthesis Mechanism and the Electrochemical Property of Polyaniline

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Chinese Journal of Polymer Science 2025年第2期43卷 328-340页

作者： Shuo Yan Zi-Fang Guo Zhi-Qing Ge bao-Ying Zhang Ya-Ru Zhang Yi-Shao Li Mo-zhen Wang Xue-Wu Ge State Key Laboratory of Precision and Intelligent Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei230026China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei230026China

As a highly promising conductive polymer material,the synthesis method,structure regulation,and performance improvement of polyaniline(PANI)are hot research *** this work,the radiation-induced polymerization of aniline in HNO_(3)solution was successfully achieved at room temperature without the use of chemical *** the analysis of the radiation chemical reactions of inorganic acids and nitrate salt solutions,the characterization of the intermediate free radicals in the irradiated systems,and the influence of the pH of the solutions on the polymerization activity and product morphologies,the radiation-induced polymerization mechanism of aniline is discussed in detail and *** at a condition of[HNO_(3)]>[aniline],i.e.,pH<2.5,PANI can be successfully obtained underγ-ray *** polymerization begins with the oxidation of aniline cations to aniline cation radicals by·NO_(3)generated by radiolysis reactions,and undergoes repeated three steps of monomer free radical recombination,deprotonation,and oxidation reaction of·NO_(3),thus forming a PANI *** addition to the polymerization reaction,the aniline units are protonated and oxidized because of the strongly acidity and oxidation of the reaction system under γ-ray irradiation,which means that the molecular chain structure of the radiation-synthesized PANI can be regulated by pH,nitrate concentration,and irradiation ***-synthesized PANI has a moderate protonation and oxidation state,which can be used for the preparation of PANI supercapacitors with better electrochemical properties than those prepared by chemical oxidation under the same *** work presents a new radiation-synthesis method and polymerization mechanism of PANI,which not only expands the application of radiation technique in the field of polymer synthesis,but also provides a new idea for the structural regulation and electrochemical property optimization of PANI.

关键词： Aniline Radiation-induced polymerization Polyaniline Electrochemical property

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Evidence-based control of stress response on intraoperative physiological indexes and recovery of patients undergoing gastrointestinal surgery

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World Journal of Gastroenterology 2025年第8期31卷 72-81页

作者： Chen-Dong Yuan bao-Zhu Zhou Ning-Yan Wang Qing-Qing Wan zhen-zhen Hu Department of General Surgery The First Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Department of Anesthesiology The First Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Although the 2021 Chinese Clinical Practice Guidelines for Enhanced Recovery after Surgery(ERAS)provide recommendations for ERAS in gastrointestinal surgery,the clinical application of standard ERAS nursing models is challenging due to the variety of diseases involved in gastrointestinal surgery and the com-plex factors contributing to patient stress ***,stress responses are more severe in older adult ***,precision medicine is required to improve the quality of nursing care and promote postoperative recovery in gastrointestinal *** demonstrate nursing benefits through clinical *** This randomized clinical trial first established an evidence-based nursing ERAS protocol in older adult patients based on literature related to perioperative nursing measures for gastrointestinal surgery stress ***,392 older adult patients who underwent gastrointestinal surgery and were admitted to our hospital between December 2021 and June 2023 were categorized into two groups to receive evidence-based(study group)or conventional(control group)ERAS nursing models,*** physiological parameters during surgery and postoperative recovery indicators were compared between the *** Among 64 domestic and international studies,the stress responses of older adult patients mainly included emotional anxiety,sleep disorders,gastrointestinal discomfort,physical weakness,pain,and *** appropriate nursing interventions included comprehensive psychological counseling,pre-and postoperative nutritional support,temperature control,pain management,and rehabilitation *** with the control group,the study group showed lower heart rate,mean arterial pressure,blood glucose level,and adrenaline level;shorter duration of drainage tube placement,time to first flatus,time to first ambulation,and postoperative hospital stay;lower anxiety scores on postoperative day 3;and lower incidences of po

关键词： Older adult patients Gastrointestinal surgery Stress response Evidence-based nursing Enhanced recovery after surgery

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4-Octyl itaconate inhibits synovitis in the mouse model of post-traumatic osteoarthritis and alleviates pain

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Chinese Journal of Traumatology 2025年第1期28卷 50-61页

作者： Yu-zhen Tang Wan Chen bao-Yun Xu Gang He Xiu-Cheng Fan Kang-Lai Tang Department of Orthopaedics/Sport Medicine Center First Affiliated Hospital of Army Medical UniversityChongqing400000China Department of Training Injury Prevention Army Medical UniversityChongqing400000China

Purpose:To investigate the pathological changes of the synovium in mice with post-traumatic osteoarthritis(PTOA)treated with 4-octyl itaconate(4-OI)and evaluate the therapeutic effects of ***:In the phenotypic validation experiment,the mice were randomly divided into 3 groups:wildtype(WT)group,sham group,and destabilization of the medial meniscus(DMM)*** MRI,micro-CT,and histological analysis,it was determined that the DMM surgery induced a mouse PTOA model with significant signs of *** 12 weeks post-DMM surgery,synovial tissues from the DMM group and WT group mice were collected for ribonucleic acid sequencing *** the 4-OI treatment experiment,mice were randomly divided into the sham group,DMM group,DMM+4-OI(50 mg/kg)group,and DMM+4-OI(100 mg/kg)*** Frey tests and open field tests were conducted at intervals during the 12 weeks following the DMM *** 12 weeks of surgery,the efficacy of 4-OI treatment on PTOA in mice was evaluated using MRI,micro-CT,histological analysis,and quantitative real-time polymerase chain ***,we utilized network pharmacology analysis to predict the mechanism of 4-OI in treating PTOA synovitis and conducted preliminary *** analysis was performed using one-way ANOVA and the Kruskal-Wallis *** was considered statistically significant at p<***:The DMM surgery effectively induced a PTOA mouse model,which displayed significant symptoms of *** symptoms included a notable increase in both the number of calcified tissues and osteophytes(p<0.001),an enlargement of the calcified meniscus and synovial tissue volume(p<0.001),and thickening of the synovial lining layer attributable to M1 macrophage accumulation(p=0.035).Additionally,we observed elevated histological scores for synovitis(p<0.001).Treatment with 4-OI inhibited the thickening of M1 macrophages in the synovial lining layer of PTOA mice(p<0.001)and reduced fibrosis in the synovi

关键词： 4-Octyl itaconate Synovitis Post-traumaticosteoarthritis Pain

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Systemic thrombosis with prothrombin Belgrade mutation in a Chinese patient:A case report

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World Journal of Clinical Cases 2025年第10期13卷 35-39页

作者： Yan-Feng Wu Yan Huang bao-Hui Weng Shan Deng Li-Ya Pan zhen Li Department of Neurology Liuzhou Worker’s HospitalLiuzhou 545007Guangxi Zhuang Autonomous RegionChina Department of Neurology Tianjin Medical University General HospitalTianjin 300052China

BACKGROUND Thrombophilia contributes to a significant increased risk of venous thromboembolism and can be either inherited or *** thrombophilia may arise from various gene mutations,some of which have not even been adequately reported or poorly *** studies reported a rare and novel missense mutation in the prothrombin gene(***596Gln),known as prothrombin *** mechanisms and therapeutic strategies associated with prothrombin Belgrade mutation have not been fully *** SUMMARY We present the case of a 26-year-old woman with recurrent systemic thrombosis induced by prothrombin Belgrade *** patient suffered from cerebral venous sinus thrombosis that rapidly progressed to systemic thrombosis,alongside a family history of cerebral thrombosis,and no traditional risk factors or abnormal coagulation ***-genome sequencing detected a novel and rare heterozygous prothrombin missense mutation,c.1787G>T(***596Gln),which was responsible for the major etiology of the systemic *** This case strengthens our understanding about hereditary basis of thrombophilia and provokes considerations for therapeutic options on prothrombin Belgrade mutation.

关键词： Arg596Gln Belgrade mutation Thrombophilia Prothrombin Case report

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内质网应激在氧化低密度脂蛋白诱导的人RPE细胞凋亡中的作用

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国际眼科杂志 2020年第10期20卷 1688-1692页

作者：吴桐党宽荣苏静波吕葆真陆新婷惠延年杜红俊空军军医大学西京医院眼科全军眼科研究所中国陕西省西安市710032

目的:观察内质网应激(ERS)在氧化低密度脂蛋白(OxLDL)诱导的人视网膜色素上皮(RPE)细胞凋亡中的作用。方法:人RPE细胞系ARPE19采用低糖DMEM培养基和10%胎牛血清进行常规培养。实验分为3组:对照组(常规培养的ARPE19)、OxLDL组(加入5、10... 详细信息

目的:观察内质网应激(ERS)在氧化低密度脂蛋白(OxLDL)诱导的人视网膜色素上皮(RPE)细胞凋亡中的作用。方法:人RPE细胞系ARPE19采用低糖DMEM培养基和10%胎牛血清进行常规培养。实验分为3组:对照组(常规培养的ARPE19)、OxLDL组(加入5、10、25、50、100μg/mL OxLDL)和LDL组(加入5、10、25、50、100μg/mL LDL)培养24h。分别采用细胞计数试剂盒(CCK8)检测各组细胞活性,流式细胞仪检测凋亡比例,蛋白质印迹(Western blotting)检测ERS相关蛋白及凋亡相关酶的表达。激光共聚焦显微镜观察人RPE细胞吞噬红色荧光探针Dil标记的氧化低密度脂蛋白(Dil-OxLDL)情况。结果:CCK8结果显示:对照组细胞存活率为(100±5.637)%,加入5、10、25、50、100μg/mL OxLDL后细胞活力分别为(105.298±9.395)%、(97.106±5.417)%、(77.015±4.055)%、(67.613±3.853)%和(43.872±9.532)%(P0.05)。流式细胞仪结果显示浓度为25μg/mL的OxLDL会明显诱导细胞凋亡,对照组、OxLDL组(25μg/mL)和LDL(25μg/mL)组凋亡率分别是(5.271±0.519)%、(41.23±1.686)%和(13.07±2.579)%(P0.05)。结论:ERS参与了OxLDL诱导的人RPE细胞凋亡,调控ERS可能抑制人RPE细胞凋亡,从而治疗RPE细胞凋亡相关疾病。

关键词：年龄相关性黄斑变性视网膜色素上皮氧化应激内质网应激凋亡

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数字共享型老年友好社会构建

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东南大学学报(哲学社会科学版) 2025年第1期27卷 105-114,151,152页

作者：代宝珍付方腾东南大学公共卫生学院江苏南京210009 东南大学老龄文明智库江苏南京210024

数字共享型老年友好社会的价值培育在“冲突—调适—平衡”的循环中更迭焕新。消弭数字鸿沟,让处于不同发展阶段的成员共享数字经济发展成果,是推进数字共享型老年友好社会治理和拓展新质生产力的关键,也是老龄文明和全龄友好的进步。... 详细信息

数字共享型老年友好社会的价值培育在“冲突—调适—平衡”的循环中更迭焕新。消弭数字鸿沟,让处于不同发展阶段的成员共享数字经济发展成果,是推进数字共享型老年友好社会治理和拓展新质生产力的关键,也是老龄文明和全龄友好的进步。将数字共享型老年友好社会的构建置于中国情境下,勾勒其社会缘起和认知实践轨迹,分析其概念意涵和特质属性,梳理我国数字共享型老年友好社会的现实表征,剖析当前面临的老龄数字建设需求高涨与供给匹配错位、数字化支撑老龄社会治理乏力、老龄事业治理的国内外环境趋于复杂等挑战,并提出我国数字共享型老年友好社会的构建路径:以切实满足老年人口需求为基本出发点构建数字共享型老年友好社会,以“共建、共治、共享”新理念推动老年友好社会治理迈向数字新阶段,以数字基层治理为关键抓手扎实提升老年友好社会治理能力,以新发展格局赋予数字共享型老年友好社会治理新机遇。

关键词：数字共享老年友好社会社会治理积极老龄化

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基于车辆轨迹预测与宏微观指标聚合的临近交叉口路段交通运行风险综合评估

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东南大学学报(自然科学版) 2025年第1期55卷 230-238页

作者：鲍琼吴臻陈建明沈永俊韩天园东南大学交通学院南京211189 苏州万集车联网技术有限公司苏州215000

针对城市道路临近交叉口路段的复杂交通流运行特点,提出了一种基于车辆轨迹预测与宏微观指标聚合的实时交通运行风险综合评估方法。首先,构建了基于长短时记忆网络的驾驶人意图识别模型,以实现车辆轨迹的准确预测。然后,基于预测的轨迹... 详细信息

针对城市道路临近交叉口路段的复杂交通流运行特点,提出了一种基于车辆轨迹预测与宏微观指标聚合的实时交通运行风险综合评估方法。首先,构建了基于长短时记忆网络的驾驶人意图识别模型,以实现车辆轨迹的准确预测。然后,基于预测的轨迹数据,提取交通流宏观运行指标和车辆微观行驶风险指标,采用数据包络分析方法构建了临近交叉口路段交通运行风险综合评价模型。最后,对比基于实际数据和预测数据的风险评价结果,以验证模型的有效性。结果表明,该方法在高风险等级的召回率为0.955,评价结果差异较小,能够有效预测交通流运行风险,为临近交叉口路段的交通安全主动管控提供了有力的决策依据。

关键词：临近交叉口路段运行风险评价指标风险预测

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Cross-species single-cell transcriptomics reveals neuronal similarities and heterogeneity in amniote pallium

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Zoological Research 2025年第01期 193-208页

作者： Fu-bao-Qian Huang Kuo Liao Yu-Nong Sun Zi-Hao Li Yan-Ru Zhang Ping-Fang Liao Si-Yuan Jiang Zhi-Yong Zhu Duo-Yuan Chen Ying Lei Shi-Ping Liu You-Ning Lin zhen-Kun Zhuang School of Biology and Biological Engineering South China University of Technology BGI Research College of Life Sciences University of Chinese Academy of Sciences

The amniote pallium, a vital component of the forebrain,exhibits considerable evolutionary divergence across species and mediates diverse functions, including sensory processing, memory formation, and learning. However, the relationships among pallial subregions in different species remain poorly characterized, particularly regarding the identification of homologous neurons and their transcriptional signatures. In this study, we utilized singlenucleus RNA sequencing to examine over 130?000 nuclei from the macaque（Macaca fascicularis） neocortex,complemented by datasets from humans（Homo sapiens）,mice（Mus musculus）, zebra finches（Taeniopygia guttata）,turtles（Chrysemys picta bellii）, and lizards（Pogona vitticeps）,enablingcomprehensivecross-species comparison. Results revealed transcriptomic conservation and species-specific distinctions within the amniote pallium. Notable similarities were observed among cell subtypes, particularly within PVALB+inhibitory neurons,which exhibited species-preferred subtypes. Furthermore,correlations between pallial subregions and several transcription factor candidates were identified, including RARB, DLX2, STAT6, NR3C1, and THRB, with potential regulatory roles in gene expression in mammalian pallial neurons compared to their avian and reptilian counterparts. These results highlight the conserved nature of inhibitory neurons, remarkable regional divergence of excitatory neurons, and species-specific gene expression and regulation in amniote pallial neurons. Collectively,these findings provide valuable insights into the evolutionary dynamics of the amniote pallium.

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