检索结果-南通市图书馆

Establishment of Cell Free Conversion System With Biotin-labelled Recombinant PrP^(sen) Expressed in ***

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2006年第3期19卷 214-218页

作者： JIN zhang JIAN-MEI GAO FENG LI JUN HAN LAN CHEN bao-yun zhang XIAO-FAN WAMG WEI ZHOU YONG LIU XIAO-PING DONG School of Medicine Xi＇an Jiaotong University Zhuque Street 205 Xi ＇an 710061 China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Yingxin Rd 100 Beijing 100052 China National Laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical College Dongdan Santiao 5 Beijing 100005 China

Objective To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope. Methods A hamster PrP protein （HaPrP） was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrP^sen preparation from scrapie strain 263K. Results Protease-resistant bands were detected after four-day incubation. Conclusion The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.

关键词： Transmissible spongiform eneephalopathies Prion Biotin Cell free conversion E. coli

Establishment of a Stable PrP^(Sc) Panel from Brain Tissues of Experimental Hamsters with Scrapie Strain 263K

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2009年第2期22卷 151-156页

作者： bao-yun zhang CHAN TIAN JUN HAN CHEN GAO QI SHI JIAN-MING CHEN HUI-YING JIANG WEI ZHOU AND XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Objective To establish a stable PrP^Sc panel from brain tissues of experimental hamsters infected with scrapie agent 263K for evaluating diagnostic techniques of human and animals＇ prion diseases. Methods Thirty brain tissue samples from hamsters intracerebrally infected with scrapie strain 263K and another 30 samples from normal hamsters were selected to prepare 10%, 1%, and 0.5% brain homogenates, which were aliquoted into stocks. PrP^Sc in each brain homogenate was determined by proteinase K digestions followed by Western blot assay and partially by immunohistochemistry. Stability and glycoforms of PrP^Sc were repeatedly detected by prp^SC-specific Western blots in half a year and 3 years later. Results PrPsc signals were observed in all 10% brain homogenates of infected hamsters. Twenty out of 30 stocks and 19 out of 30 stocks were PrPsc positive in 1% and 0.5% brain homogenatesof infected hamsters, respectively. TWenty-seven out of 30 stocks presented three positive bands in 10% brain homogenates, whereas none of 1% and 0.5% homogenates contained 3 bands. The detection of prpSc-specific signals stored in half a year and 3 years later demonstrated that the ratio of PrPsc positive samples and glycoforms was almost unchanged. All normal hamsters＇ brain homogenates were PrP^Sc negative. Conclusion A PrP^Sc panel of prion disease can be established, which displays reliably stable prp^Sc-specific signals and glycoforms.

关键词： TSE prp^Sc Panel Glycoforms Stability

T188K-Familial Creutzfeldt–Jacob Disease, Predominant Among Chinese, has a Reactive Pattern in CSF RT-QuIC Different from D178N-Fatal Familial Insomnia and E200K-Familial CJD

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2019年第3期35卷 519-521页

作者： Kang Xiao Qi Shi Wei Zhou bao-yun zhang Yuan Wang Cao Chen Yue Ma Chen Gao Xiao-Ping Dong State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China

Dear Editor,Human prion diseases consist of sporadic, genetic/familial, and acquired forms. The familial form accounts for 5%-15% of all human prion diseases, including familial Creutzfeldt- Jacob disease (fCJD), Gerstmann-Straussler-Scheinker syndrome, and fatal familial insomnia (FFI)[1-3]. All genetic prion diseases are directly associated with mutations (point-mutation or insertion) in the PRNP gene located on human chromosome 20 and encodes prion protein (PrP). So far,>55 mutations in the PRNP gene have been described [4]. Some PRNP mutations and their related genetic prion diseases have been reported worldwide, while others show clear region- or ethnicity-associated features.

关键词： Human prion diseases sporadic ethnicity-associated features

Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2005年第4期18卷 273-280页

作者： XIN-LI XIAO HUI-YING JIANG JIN zhang JUN HAN KAI NIE XIAO-BO ZHOU YIN-XIA HUANG LAN CHEN WEI ZHOU bao-yun zhang YONG LIU XIAO-PING DONG School of Medicine Xi＇an Jiao-Tong University Zhu-Que Street 205 Xi＇an 710061 Shaanxi China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Hngxin Rd. 100 Beijing 100052 China National Laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical College Dong Dan San Tiao 5 Beijing 100005 China

To prepare the PrP specific monoclonal antibodies （mAbs） that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein （HaPrP）. Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrP^Sc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPc from several other mammalian species, including humans and cattles, lmmunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPso. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.

关键词： Prions Hamster prion protein Monoclonal antibodies

Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2007年第3期20卷 198-202页

作者： YAN-JUN GUO JUN HAN HAI-LAN YAO bao-yun zhang JIAN-MEI GAO JIN zhang XIN-LI XIAO XIAO-FAN WANG WEI-QIN ZHAO DE-XIN WANG XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Department of Neurology Beijing Friendship HospitalCapital Medical University Beijing 100050 China

Objective To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K. Methods Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots. The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted. PrP^5c in brain tissues of the infected animals was detected by PrP specific Western blot assays. Results Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner. Compared with the control group after incubated for 53.75±0.50 days, the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5±1.73 and 59.5±0.58 days （P〈0.05）. Conclusion Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.

关键词： Scrapie Prion Tetracycline Protease-resistant activity Animal experiment

Establishment of a Sandwich ELISA Method for Detection of Vascular Endothelial Growth Factor in Serum Samples of Hepatocellular Carcinoma Patients

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2008年第1期21卷 69-74页

作者： BING SHAN CHEN GAO JIAN-MING CHEN XIN-Yu BI bao-yun zhang YAN GUO CHEN-FANG DONG RUN AN QI SHI JING-QUN HU PING ZHAO JUN HAN XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences Beijing 100021 China

Objective To establish a sandwich ELISA method for detecting vascular endothelial growth factor （VEGF） in sera of population and the patients with hepatocellular carcinoma （HCC）. Methods Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF proteins were expressed and purified from E. coli in His-Trx or GST fusion forms. The specific VEGF antibodies were elicited in experimental rabbits and mice by immunization of the full length VEGF fusion protein His-Trx-VEGF1-165. After purification of antibodies with chromatograph of Protein G, a sandwich ELISA technique was established. Serum VEGF levels were evaluated in 229 adults and 291 HCC patients. Results SDS-PAGE displayed that the molecular weights of the expressed full length （His-Trx-VEGF1-165）, N-terminal （His-Trx-VEGF1-100） and C-terminal （GST-VEGF100-165） human VEGF fusion proteins were about 38KD, 31KD, and 33KD, respectively. Western blots confirmed that the prepared antisera were able to recognize both prokaryoticly and eukaryoticly expressed recombinant VEGF proteins. Assays of serially diluted His-Trx-VEGF1-100 by the established sandwich ELISA method showed that the linear range of the standard curve was 0.625-320 ng/mL, with the squared correlation coefficient R^2=0.991. Screening of a serum panel containing 291 serum samples of HCC patients and 229 health adults revealed that the average VEGF level in HCC patients was higher than that in healthy controls, with a statically significant difference. Conclusion The established sandwich ELISA reflects the level of serum VEGF and provide scientific basis for screening metastasis and recurrence of HCC using serum VEGF as an index.

关键词： Hepatocellular carcinoma Vascular endothelial growth factor Enzyme-linked immunosorbent assay

Visible-light-responsive homologous heterostructure of oxynitrides constructed to boost charge separation and water splitting

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2025年第1期68卷 186-191页

作者： Hai Zou yunfeng bao Xueshang Xin Yu Qi Tengfeng Xie Fuxiang zhang State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University College of Chemistry Jilin University

Constructing type II heterostructures represents a widely recognized strategy for enhancing charge separation and photocatalytic efficiency,but it remains challenging to fabricate homologous heterostructures based on visible-light-responsive semiconductors,especially for popularly investigated （oxy）nitrides with low thermal ***,we synthesized a homologous heterostructure （denoted as YTON＿H） based on the oxynitrides of YTaO4-xNyand YTa *** heterostructure exhibits type II band structure alignment and intimate interface achieved through a magnesium （Mg） powder-assisted nitridation *** with widely reported heterologous heterostructures,the as-obtained YTON＿H homologous heterostructure could similarly exhibit enhanced charge separation as well as photocatalytic water splitting performance with respect to its counterparts(YTaO4-xNyand YTaON2).Meanwhile,detailed structural characterizations and spectroscopy analysis have been made to confirm the formation of heterostructure and promotion of spatial charge separation.

关键词： charge separation heterostructure oxynitride photocatalysis visible light

Mesh-supported V2O5-WO3/TiO2 nanosheet array catalysts for efficient removal of NOx

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Tungsten 2025年第1期7卷 100-111页

作者： Zhi-Qi zhang Liu Yang Wen Luo Vladimir An Jing Li bao-Dan Liu School of Materials Science and Engineering Northeastern University Foshan Graduate School of Innovation Northeastern University Kizhner Research Center School of Advanced Manufacturing Technologies Tomsk Polytechnic University

The design and synthesis of vanadium-based denitration catalysts with low-temperature activity pose significant challenges in the field of toxic gas *** this study,we have developed a novel approach to fabricate highly efficient V2O5-WO3/TiO2monolithic mesh catalysts for NOxreduction at low ***,TiO2nanosheets with strong adhesion and large surface area were grown on Ti mesh using plasma electrolytic oxidation technology and hydrothermal ***,super-small traces of WO3and V2O5active nano-species were subsequently deposited on TiO2nanosheets using the deposition-precipitation *** to traditional ceramic-based V2O5-WO3/TiO2catalysts,our V2O5-WO3/TiO2monolithic mesh catalyst exhibits superior mechanical performance,excellent thermal conductivity,lower pressure drop,higher utilization of active components,and stronger synergistic ***,the V2O5-WO3/TiO2monolithic mesh catalysts exhibited excellent low-temperature catalytic activity with 98% of NOxconversion at 220℃,and remarkable long-term SO2tolerance and water resistance in the selective catalytic reduction（SCR） of NOxwith *** SCR reaction of tiny V2O5and WO3active species supported on TiO2nanosheets directly demonstrated to follow the Eley-Rideal mechanism by in situ infrared spectra.

关键词： Low temperature activity Monolithic mesh catalysts NH3-SCR SO2 tolerance V2O5-WO3/TiO2

Coordinated Control Strategy of New Energy Power Generation System with Hybrid Energy Storage Unit

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Energy Engineering 2025年第1期122卷 167-184页

作者： yun zhang ZifenHan Biao Tian Ning Chen Yi Fan School of Mechano-Electronic Engineering Xidian UniversityXi’an710071China Electric Power Control Center State Grid Gansu Electric Power CompanyLanzhou730030China Electric Power Control Center State Grid Gansu Electric Power Company Zhangye Power Supply BranchZhangye734000China

The new energy power generation is becoming increasingly important in the power *** as photovoltaic power generation has become a research hotspot,however,due to the characteristics of light radiation changes,photovoltaic power generation is unstable and random,resulting in a low utilization rate and directly affecting the stability of the power *** solve this problem,this paper proposes a coordinated control strategy for a newenergy power generation system with a hybrid energy storage unit based on the lithium iron phosphate-supercapacitor hybrid energy storage ***,the variational mode decomposition algorithm is used to separate the high and low frequencies of the power signal,which is conducive to the rapid and accurate suppression of the power fluctuation of the energy storage ***,the fuzzy control algorithm is introduced to balance the power between energy *** this paper,the actual data is used for simulation,and the simulation results show that the strategy realizes the effective suppression of the bus voltage fluctuation and the accurate control of the internal state of the energy storage unit,effectively avoiding problems such as overshoot and over-discharge,and can significantly improve the stability of the photovoltaic power generation systemand the stability of the Direct Current *** is of great significance to promote the development of collaborative control technology for photovoltaic hybrid energy storage units.

关键词： Photovoltaic power suppression hybrid energy storage unit variationalmodal decomposition fuzzy control power distribution control