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Microdissection of chromosome 7B of common wheat and cloning of low-copy specific DNA sequences

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Chinese Science Bulletin 1999年第7期44卷 632-636页

作者： bao liu Junkang Rong Yingshan Dong Fangpu Han Zhenlan liu Mengyuan He Baiqu Huang Shui Hao NE Normal Univ Inst Cytol & Genet Changchun 130024 Peoples R China Weizmann Inst Sci Dept Plant Genet IL-76100 Rehovot Israel

The 7B chromosome of common wheat was microdissected from pollen mother cells of the 7B monosomic line of common wheat cv. Chinese Spring (CS). After proteinase K and DNA topoisomerase Ⅰtreatments, the isolated chromosomes were subjected to 1—3 rounds of DOPPCR amplification, which produced continuous DNA fragments ranging from 150 to 700 bp. Genomic Southern hybridization confirmed that the PCR products were originated from the wheat genome. Cloning of portion ( > 200 bp) of the 3rd round DOP-PCR products (50 μL) could generate about 20 000 recombinant clones. Characterization of 50 randomly chosen clones indicated that 21 clones produced discrete PCR products with the size of 240—600 bp. Dot-blot hybridization showed that among the 21 clones, 11 (～ 55%) were of low-copy nature while 10 (～45%) were repetitive. Southern hybridization with the complete set of the CS 'nullisomic-tetrasomic (NT)' lines demonstrated that all the 6 low-copy clones were specific to either chromosome 7B or the 7th

关键词： wheat chromosome microdissection DOP-PCR specific DNA sequences.

Anemia and risk of periprocedural cerebral injury detected by diffusion-weighted magnetic resonance imaging in patients undergoing transcatheter aortic valve replacement

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World Journal of Emergency Medicine 2022年第1期13卷 32-39页

作者： Stella Ng Qi-feng Zhu Ju-bo Jiang Chun-hui liu Jia-qi Fan Ye-ming Xu Xian-bao liu Jian-an Wang Department of Cardiology the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Zhejiang University School of Medicine Hangzhou 310058China

BACKGROUND: Anemia is prevalent in patients undergoing transcatheter aortic valve replacement(TAVR) and has been linked to impaired outcomes after the procedure. Few studies have evaluated the impact of anemia and new ischemic lesions post ***: We prospectively enrolled 158 patients who received TAVR in our center. Anemia was defined according to the World Health Organization criteria as hemoglobin <12 g/dL in women and <13 g/dL in men. All patients underwent diffusion-weighted magnetic resonance imaging(DW-MRI) procedure before and within 4–7 days after TAVR. RESULTS: Anemia was present in 85(53.8%) patients who underwent TAVR, and 126(79.7%) patients had 718 new DW-MRI positive lesions with a mean of 4.54±5.26 lesions per patient. The incidence of new ischemic lesions was 81.2% in patients with anemia versus 78.1% in patients without anemia(P=0.629). Moreover, anemic patients had bigger total volume/lesions in the anterior cerebral artery/middle cerebral artery(ACA/MCA) and MCA regions compared to the non-anemic patients(31.89±55.78 mm^(3) vs. 17.08±37.39 mm^(3), P=0.049;and 54.54±74.72 mm^(3) vs. 33.75±46.03 mm^(3), P=0.034). Anemia was independently associated with the volume/lesion in the ACA/MCA(β=16.796, 95% confidence interval [95% CI] 2.001 to 31.591, P=0.026) and in the MCA zone(β=0.020, 95% CI 0.001 to 0.040, P=0.041). CONCLUSIONS: Patients with pre-procedural anemia may have bigger total volume/lesions in the ACA/MCA and MCA regions compared to the non-anemic patients. Whether the consequences of bigger total volume/lesions impact neurological and cognitive outcomes remains to be investigated.

关键词： Aortic stenosis Anemia Cerebral ischemic lesions Transcatheter aortic valve replacement

Behavior and controlling factors of methane adsorption in Jurassic continental shale,northeastern Sichuan Basin

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Energy Geoscience 2023年第1期4卷 83-92页

作者： Qian-wen Li Zhong-bao liu Fei-Ran Chen Kun Zhang Ling Tang Sinopec Petroleum Exploration and Production Research Institute Beijing102206China Sinopec Southern Company of Exploration ChengduSichuan 610041China School of Geoscience and Technology Southwest Petroleum UniversityChengduSichuan 610059China CNOOC Research Institute Ltd. Beijing100029China

The behavior and controlling factors of natural gas adsorption in the Jurassic continental shale in the northeastern Sichuan Basin are studied based on the organic geochemical features,mineral compositions and pore structure parameters through a series of experiments on samples from the *** show that the total gas content of the shale measured on-site is 0.1-5.3 cm^(3)/g,with an average of 0.7 cm^(3)/*** methane isothermal adsorption curves show a trend of increasing first and then decreasing,indicating an obvious excessive *** shale has a maximum adsorption capacity(V^(L))of 0.44-3.59 cm^(3)/g,with an average of 1.64 cm^(3)/g,lower than that of marine shale in the same *** organic matter content and pore structure characteristics are identified as the two main factors controlling the adsorption capacity of the *** in the shale are the main storage space for gas to be *** to well developed shell laminae and interlayers in the shale,calcite plays a more important role than clay minerals in affecting the adsorption of gas to the *** formation temperature and water content also significantly inhibit the gas adsorption to the *** with marine shale in the basin,the Jurassic continental shale is more heterogeneous and lower in TOC ***,with a more widely developed clayey shale lithofacies and shell limy shale lithofacies as well as relatively less developed organic pores and micropores,the continental shale is inferior to marine shale in terms of gas adsorption capacity.

关键词： Methane adsorption Controlling factors Continental shale Jurassic Sichuan basin

RESEARCH IN ENERGY SYSTEMS ENGINEERING IN CHINA—A SURVEY

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天津大学学报 1982年第4期 123-133页

作者： liu bao

In this paper the recent research results in energy systems engineering in China are surveyed. According to the different research projects in energy systems engineering field have been done or under way in China, this brief survey is divided into three parts. The first part is the research in energy demand and supply forecasting. The second part is the research of energy models, namely, departmental energy models, regional energy models, national energy models and other energy models. The third part is the research of decision methodology.

关键词： A SURVEY RESEARCH IN ENERGY SYSTEMS ENGINEERING IN CHINA

An alternative approach to match field production data from unconventional gas-bearing systems

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Petroleum Science 2020年第5期17卷 1370-1388页

作者： Zhi-Gang Zhang Yan-bao liu Hai-Tao Sun Wei Xiong Kai Shen Quan-Bin Ba National Key Laboratory of Gas Disaster Detecting Preventing and Emergency ControllingChongqing 400037China Chongqing Research Institute of China Coal Technology and Engineering Group Crop. Chongqing 400037China School of Resources and Safety Engineering Chongqing UniversityChongqing 400045China

Nowadays,the unconventional gas-bearing system plays an increasingly important role in energy *** performances of the current history-matching techniques are not satisfied when applied to such *** overcome this shortfall,an alternative approach was developed and applied to investigate production data from an unconventional gas-bearing *** this approach,the fluid flow curve obtained from the field is the superposition of a series of Gaussian *** automatic computing program was developed in the MATLAB,and both gas and water field data collected from a vertical well in the Linxing Block,Ordos Basin,were used to present the data processing *** the reservoir study,the automatic computing program was applied to match the production data from a single coal seam,multiple coal seams and multiple vertically stacked reservoirs with favourable fitting *** with previous approaches,the proposed approach yields better results for both gas and water production data and can calculate the contributions from different *** start time of the extraction for each gas-containing unit can also be *** new approach can be applied to the field data prediction and designation for the well locations and patterns at the reservoir scale.

关键词： Gaussian function Flow rate Unconventional gas Ordos Basin Gas-bearing reservoirs

Leucospilotaside C,a new sulfated triterpene glycoside from sea cucumber Holothuria leucospilota

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Chinese Chemical Letters 2008年第12期19卷 1462-1464页

作者： Hua Han Yang Hua Yi bao Shu liu Xiao Hua Wang Min Xiang Pan Research Center for Marine Drugs School of Pharmacy Second Military Medical University Shanghai 200433 China College of Animal Sciences Zhejiang University Hangzhou 310029 China

A new triterpene glycoside, leucospilotaside C, along with two known saponin, was isolated from sea cucumber Holothuria leucospilota collected from the South China Sea, and its structure was elucidated as 3-0-{4′-O-sodiumsulfate-β′-D-xylopyranosyl}- holosta-22,25-epoxy-9-ene-3β,12α,17α-triol （1） by extensive spectroscopic analysis and chemical methods. The glycosides have the same triterpene aglycone, but differ in the oligosaccharide moieties.

关键词： Holothuria leucospilota Triterpene glycoside Leucospilotaside C

Mesenterico-portal vein invasion should be an important factor in TNM staging for pancreatic ductal adenocarcinoma: Proposed modification of the 8th edition of the American Joint Committee on Cancer staging system

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World Journal of Gastroenterology 2019年第46期25卷 6752-6766页

作者： Hong-Yu Chen Xing Wang Hao Zhang Xu-bao liu Chun-Lu Tan Department of Pancreatic Surgery West China HospitalSichuan University

BACKGROUND The 8^th edition of the American Joint Committee on Cancer(AJCC)staging system for pancreatic ductal adenocarcinoma(PDAC)excludes extrapancreatic extension from the assessment of T stage and restages tumors with mesenterico-portal vein(MPV)invasion into T1-3 diseases according to tumor ***,MPV invasion is believed to be correlated with a poor *** To analyze whether the inclusion of MPV invasion can further improve the 8th edition of the AJCC staging system for *** This study retrospectively included 8th edition AJCC T1-3N0-2M0 patients undergoing pancreaticoduodenectomy/total pancreatectomy from two cohorts and analyzed survival *** the first cohort,a total of 7539 patients in the surveillance,epidemiology,and end results database was included,and in the second cohort,689 patients from the West China Hospital database were *** Cox regression analysis showed that MPV invasion is an independent prognostic factor in both *** the MPV-group,all pairwise comparisons between the survival functions of patients with different stages were significant except for the comparison between patients with stage IIA and those with stage ***,in the MPV+group,pairwise comparisons between the survival functions of patients with stage IA,stage IB,stage IIA,stage IIB,and stage III were not significant.T1-3N0 patients in the MPV+group were compared with the T1N0,T2N0,and T3N0 subgroups of the MPV-group;only the survival of MPV-T3N0 and MPV+T1-3N0 patients had no significant *** comparisons of patients with stage IIA and subgroups of stage IIB showed(1)no significant difference between the survival of T2N1 and T3N0 patients;(2)a longer survival of T1N1 patients that was shorter than the survival of T2N0 patients;and(3)and a shorter survival of T3N1 patients that was similar to that of T1-3N2 *** The modified 8th edition of the AJCC staging system for PDAC proposed in this study,which i

关键词： Pancreatic ductal adenocarcinoma Portal vein Mesenteric veins Neoplasm staging Pancreaticoduodenectomy

Duodenum and ventral pancreas preserving subtotal pancreatectomy for low-grade malignant neoplasms of the pancreas: An alternative procedure to total pancreatectomy for low-grade pancreatic neoplasms

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World Journal of Gastroenterology 2017年第35期23卷 6457-6466页

作者： Xing Wang Chun-Lu Tan Hai-Yu Song Qiang Yao Xu-bao liu Department of Pancreatic Surgery West China HospitalSichuan University West China School of Public Health Sichuan University

AIM To describe the indications, technique and outcomes of the novel surgical procedure of duodenum and ventral pancreas preserving subtotal pancreatectomy(DVPPSP).METHODS Data collected retrospectively from 43 patients who underwent DVPPSP and TP between 2009 and 2015 in our single centre were analysed. For enrolment, only patients with low-grade pancreatic neoplasms, such as pancreatic neuroendocrine tumors, intraductal papillary mucinous neoplasms(IPMNs), and solid pseudopapillary tumors, were included. Ten DVPPSP(group 1) and 13 TP(group 2) patients were selected in this *** There were no significant differences in age, gender, comorbidities, preoperative symptoms, American Society of Anesthesiologists score or indications for surgery between the two groups. The most common indication was IPMN for DVPPSP and TP(60% vs 85%, P = 0.411). Compared with the TP group, the DVPPSP group had comparable postoperative morbidities(P = 0.405) and mortalities(both nil), but significantly shorter operative time(232 ± 19.6 min vs 335 ± 32.3 min, P < 0.001). DVPPSP preserved better long-term pancreatic function with less supplementary therapy(P < 0.001) and better quality of life(Qo L) after surgery, including better scores in social(P = 0.042) and global health(P = 0.047) on functional scales and less appetite loss(P = 0.049) on the symptom scale. CONCLUSION DVPPSP is a feasible and safe procedure that could be an alternative to TP for low-grade neoplasms arising from the body and tail region but across the neck region of the pancreas; DVPPSP had better metabolic function and Qo L after surgery.

关键词： Low-grade malignant neoplasm Ventral pancreas preserving Subtotal pancreatectomy Quality of life

Prospects and applications of enucleation in solid pseudopapillary neoplasms of the pancreas

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1227-1238页

作者： Rui Wang Jing Li Chun-Lu Tan Xu-bao liu Yong-Hua Chen Department of Pancreatic Surgery West China Hospital of Sichuan UniversityChengdu 610037Sichuan ProvinceChina Department of Operating Room West China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu 610037Sichuan ProvinceChina

Solid pseudopapillary neoplasms(SPNs)of the pancreas are rare,low-grade,malignant neoplasms that are mostly seen in young women in the second and third decades of life and are quite uncommon in *** resection for benign and borderline neoplasms of the pancreas is associated with a substantial risk of postoperative morbidity and long-term functional impairment,whereas enucleation leads to less morbidity and preserves healthy parenchyma as well as exocrine and endocrine *** of SPNs has been increasingly reported to be feasible and safe for preserving the normal physiological function of the pancreas,especially in teenagers and *** review summarizes findings published in recent years on the enucleation of SPNs as well as potential future developments and *** has undoubtedly come to stay as an alternative surgical procedure for ***,many questions remain unresolved,and future directions toward the best surgical indication,the prevention and intervention of complications,especially pancreatic fistula,intraoperative resection margin safety assessment,and long-term oncology prognosis remain to be evaluated and should be explored in future clinical trials.

关键词： Solid pseudopapillary neoplasm Enucleation Pancreatic tumor Distal pancreatectomy Pancreaticoduodenectomy