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New thiopyran-4-spiro-2′-(1,3-dithiolane):Formation mechanism and crystal structure

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Chinese Chemical Letters 2011年第3期22卷 280-283页

作者： Hong Qi li Zhong bao Zhang lin li Key Laboratory of Science & Technology of Eco-Textile Ministry of Education College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China

Ethylene trithiocarbonate reacted with dimethyl acetylenedicarboxylate to furnish tetramethyl thiopyran-4-spiro-2＇-（1,3- dithiolane）-2,3,5,6-tetracarboxylate,a new thiopyran-4-spiro-2＇-（1,3-dithiolane） heterocyclic compound,as the minor product together with the major product dimethyl 2-thioxo-1,3-dithiole-4,*** new heterocycle was probably formed through a[2 ＋ 2]cycloaddition between ethylene trithiocarbonate and dimethyl acetylenedicarboxylate followed by a 1,3-dipolar cycloaddition or[4 ＋ 2]*** structure of the new compound revealed that the thiopyran ring and 1,3-dithiolane moiety were perpendicular to each other.

关键词： Thiopyran-4-spiro-2'-(1 3-dithiolane) Cycloaddition Mechanism Crystal structure

Immunogenicity Analysis of Prokaryotic Expression Products of Kaposi's Sarcoma Associated Herpesvirus orf65

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Virologica Sinica 2008年第3期23卷 196-202页

作者： Bi-shi FU bao-lin li lin-ding WANG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

To purify the protein encoding the small capsid protein （SCP） of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi＇s sarcoma associated-herpesvirus （KSHV） was expressed in a prokaryotic expression system. The expression of recombinant *** containing pQE-80L-orf65 was induced by isopropyl-13-D-thiogalactopyranoside （IPTG） and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate- polyacrylamide gel electrophoresis （SDS-PAGE） and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An EliSA assay was established to analyze the immunogenicity of the purified orf65 protein. The EliSA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using EliSA.

关键词： Kaposi's sarcoma-associated herpesvirus (KSHV) orf65 Chromatography Immunogenicity

Highly diastereoselective cascade dearomatization of 3-(2-isocyanoethyl)indoles with nitrile imines: a facile access to unexpected polycyclic indolines

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Organic Chemistry Frontiers 2022年第5期9卷 1336-1342页

作者： Xiaohu Zhao Yuqiao Zhou bao-lin li Guangxi Du Zhipeng Yu Key Laboratory of Green Chemistry&Technology Ministry of EducationCollege of ChemistrySichuan UniversityChengdu 610064China

A highly efficient dearomatization of 3-(2-isocyanoethyl)indoles with in situ generated nitrile imines has been developed through a nucleophilic/Friedel–Crafts/aza-Mannich type cascade and 1,3-dipolar cyclo-addition reaction under catalyst-free and mild reaction *** strategy offers a novel and con-venient route to the rapid assembly of a wide range of unprecedented polycyclic indoline scaffolds in moderate to excellent yields and diastereoselectivities.

关键词： conditions nitrile indole

Existence and Exponential Stability of Almost Periodic Solution for BAM Neural Networks with Impulse

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维普期刊数据库

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Journal of Mathematical Research and Exposition 2010年第6期30卷 1039-1047页

作者： bao lin li Rong WANG College of Mathematics and Information Science Northwest Normal UniversityGansu 730070P.R.China

This paper discusses a class of the bidirectional associative memories（BAM） type neural networks with *** using the Banach fixed point theory and some analysis technology,we obtain the existence of almost periodic s... 详细信息

关键词： BAM neural networks almost periodic solution impulse stability Banach fixed point theory.

Two New Diterpenoids from Isodon rubescens

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Chinese Chemical Letters 2000年第1期11卷 43-44页

作者： bao lin li Shao Nong CHEN Zhi Xian SHI Xuan TIAN Yao Zu CHEN National Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000 Shanghai Institute of Materia Medica Academia SinicaShanghai 200031 Northwest Plateau Institute of Biology Academia SinicaXining 810001

Two new diterpenoids taibairubescensin A (1) and B (2) have been isolated from Isodon rubescens. The structures of compound 1 and 2 were elucidated as 2 beta,3 beta-diacetoxy-11 beta,13 alpha-dihydroxy-ent-kaur- 16-en-15-one (1) and 3 beta,11 beta-diacetoxy-2 beta,6 alpha-dihydroxy-ent-kaur-16-en-15-one (2) on the basis of spectroscopic analysis.

关键词： Isodon rubescens taibairubescensin A taibairubescensin B ent-kaurene diterpenoids

Establishment of an EliSA to Detect Kaposi's Sarcoma-associated Herpesvirus Using Recombinant ORF73

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Virologica Sinica 2010年第3期25卷 168-176页

作者： Xin-xing OUYANG Bi-shi FU bao-lin li Yan ZENG Fan-hong XU lin-ding WANG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Department of Microbiology Anhui Medical University Hefei 230032 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University Shihezi 832002 China Shanghai Institute of Biological Products Shanghai 200052 China

Kaposi's sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman's disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in *** and purified. The expressed recombinant ORF73 was identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). A protein of about 27 kDa was expressed as expected. Western Blotting showed that the purified recombinant ORF73 reacted with KSHV positive serum. The immunogenicity of the recombinant ORF73 was further analysed by EliSA and the optimal conditions were determined. The ORF73 EliSA was used to compare the KSHV seroprevalence between Hubei and Xinjiang Han people. The Han people in Xinjiang have significantly higher KSHV seroprevalence than their counterparts in Hubei (6.7% vs 2.9%, P = 0.005).

关键词： Kaposi's sarcoma-associated herpesvirus (KSHV) ORF73 EliSA Hubei Xinjiang Seroprevalence

Expression of Kaposi's Sarcoma-associated Herpesvirus ORFK8.1 and Its Preliminary Diagnostic Application

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Virologica Sinica 2009年第3期24卷 202-208页

作者： Bi-shi FU bao-lin li Xin-xing OUYANG Yan ZENG Fan-hong XU lin-ding WANG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China Graduate School of the Chinese Academy of Sciences Beejing 100039 China Shanghai Institute of Biological Products Shanghai 200052 China Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University Shihezi 832002 China

The ORFK8.1 of Kaposi＇s sarcoma associated-herpesvirus （KSHV） was expressed in a prokaryotic expression system. The expression of recombinant *** containing pQE-80L-orf KS.1 was induced by isopropyl-b-D-thiogalactopyranoside （IPTG）. The fusion protein was purified by chromatyography. The expressed protein and its purified product were identified by sodium dodecyl sulfate-polyacrylamide gel eletrophoresis （SDS-PAGE）. SDS-PAGE showed that a protein of 26 kDa was visualized as expected. A western blot assay was established to analyze the immunogenicity of purified recombinant ORFKS. 1 protein. The optimal condition of the recombinant ORFKS. 1 EliSA assay was confirmed： the concentration of antigen was 5 μg/mL, the dilution of serum was 1：200. We used the EliSA method to investigate the recombinant ORF KS. 1 protein＇s specificity, the data showed that the specificity of ORF KS.1 to detect KSHV was 100%. At the same time, 560 sera samples from Hubei province were detected by using ORFKS. 1 EliSA to investigate KSHV seroprevalence in this region. The KSHV seroprevalence in Hubei province is shown to be 6.80%.

关键词： Kaposi's sarcoma-associated herpesvirus (KSHV) ORFKS. 1 Enzyme-linked immunosorbent assays (EliSA) Seroprevalence

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滞后型脉冲泛函微分方程周期解的存在性

数学学报（中文版） 2023年第4期66卷 707-716页

作者：李宝麟田瑞西北师范大学数学与统计学院兰州730070

本文借助Mawhin重合度理论中的延拓定理和广义常微分方程周期解的存在性,在滞后型脉冲泛函微分方程与广义常微分方程存在等价关系的条件下,建立了滞后型脉冲泛函微分方程周期解的存在性定理.

关键词：滞后型脉冲泛函微分方程广义常微分方程 Kurzweil积分周期解拓扑度

Quantum public-key crypto via EPR pairs

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： Xiang-Qun Fu Hong-Wei li Jian-Hong Shi Tan li Wan-Su bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs