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中国作物学会2013年学术年会

作者： Yachun Su Shanshan Wang Jinlong Guo bantong xue Liping Xu Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding Ministry of Agriculture/Fujian Agriculture and Forestry University

Sporisorium scitamineum is a fungal smut pathogen epidemic in sugarcane producing *** detection and proper identification of the smut are an essential requirement in its management *** this study,we developed a TaqMan real-time PCR assay using specific primers(bEQ-F/bEQ-R) and a TaqMan probe (bEQ-P) which were designed based on the bE(b East mating-type) gene(Genbank Accession No.U61290.1). Standard curves revealed that the new TaqMan real-time PCR procedure has high amplification efficiency when applied to a range of samples,*** pbE DNA,and plant DNA extracted from diseased *** method was more sensitive(a detection limit of 10 ag pbE DNA and 0.8 ng sugarcane genomic DNA) than that of conventional PCR(10 fg and 100 ng,respectively).Reliability was demonstrated through the positive detection of samples collected from artificially-inoculated sugarcane plantlets(FN40).This TaqMan assay was capable of detecting the smut pathogen at the initial stage(12 h) of infection and suitable for inspection of sugarcane pathogen-free seed cane and ***,quantification of pathogen was verified in pathogen challenged buds in different sugarcane *** values and copy numbers showed a significant difference between the varieties of Yacheng05-179(resistant) and "ROC"22(susceptible) suggesting this method can be used for evaluation of smut resistance in different sugarcane *** process of teliospore germination and the curve of mycelial growth was observed in order to get a better determination on the key time of sugarcane-smut *** together,this novel assay can be used as a diagnostic tool for sensitive,accurate,fast and quantitative detection of the pathogen especially for asymptomatic seed cane or plants and evaluation of smut resistance of sugarcane genotypes.

关键词： Saccharum officinarum Sporisorium scitamineum TaqMan real-time PCR Standard curve Detection Quantification

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Selection of endogenous reference genes for copy number detection in transgenic sugarcane

Selection of endogenous reference genes for copy number dete...

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中国作物学会2013年学术年会

作者： bantong xue Jinlong Guo Youxiong Que Zhiwei Fu Wu Lu-guang Liping Xu Key Laboratory of Sugarcane Biology and Genetic Breeding Fujian Agriculture and Forestry UniversityMinistry of Agriculture School of Agriculture and Food Sciences University of Queensland

Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic *** real-time PCR technique,which depends on the use of appropriate endogenous reference genes,has been widely accepted as an accurate and time-saved method in the copy number detection of transgenic plants and GM detection to meet the regulatory and legislative *** date,most transgene crops have been developed corresponding endogenous reference genes,some species have multiple applicable endogenous reference genes,but limited in ***,further development of exogenous reference genes for polyploid and aneuploid sugarcane is very *** this study,we evaluated a set of potential ’single copy’ genes including P4H,APRT,ENOL,CYC,TST and PRR,through qualitative PCR and absolute quantitative PCR. On the basis of copy number comparison in different sugarcane genotypes,including 5 Saccharum officinarum, one *** and two ***,the copy number of these endogenous genes fell into three groups:a high copy number group with only ENOL③,a medium copy number group containing TST①and PRR①,and a low copy number group with P4H③,APRT②and CYC②.Besides,the most stable reference genes were P4H, APRT and CYC,while the least stable genes were ENOL and TST in different sugarcane ***, primer pairs P4H③,APRT②and CYC②were selected as the suitable reference gene prime pairs in sugarcane. The test of multi-target reference genes plasmid on revealed that the APRT gene was a specific amplicon within the detection using the primers APRT②,and thus this gene was assumed to be the most suitable endogenous reference gene in *** results should be helpful for the analyses of the copy number of exogenous genes in transgenic sugarcane,and are also of vital significance for sugarcane transgenic breeding and its commercial application.

关键词： Sugarcane Endogenous reference genes qRT-PCR Copy number

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福清市机收再生稻生产现状及其发展对策

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福建稻麦科技 2024年第1期42卷 67-70页

作者：薛扳佟福建省福清市乡村振兴发展中心福建福州350300

再生稻作为“一次播种,两季收获”的种植模式,对稳定粮食面积,提高粮食产量,增加农民种粮效益起到关键性作用。介绍了福清市机收再生稻生产现状和发展潜力,分析再生稻发展存在的问题,对进一步发展再生稻高效生产提出对策和建议。

关键词：机收再生稻生产现状发展对策

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甘蔗葡聚糖酶ScBG1基因的电子克隆及序列分析

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中国农学通报 2013年第12期29卷 80-90页

作者：薛扳佟郭晋隆苏亚春罗俊游倩阙友雄福建农林大学/农业部福建甘蔗生物学与遗传育种重点实验室福州350002

为了揭示甘蔗β-1,3-葡聚糖酶基因的结构特点和功能特性,从而为该基因的研究和应用提供基础。以水稻β-1,3-葡聚糖酶基因（U72255.1）为搜寻探针,运用电子克隆技术,获得一条甘蔗β-1,3-葡聚糖酶基因全长cDNA,命名为ScBG1,并对其进行生... 详细信息

为了揭示甘蔗β-1,3-葡聚糖酶基因的结构特点和功能特性,从而为该基因的研究和应用提供基础。以水稻β-1,3-葡聚糖酶基因（U72255.1）为搜寻探针,运用电子克隆技术,获得一条甘蔗β-1,3-葡聚糖酶基因全长cDNA,命名为ScBG1,并对其进行生物信息学分析。结果表明：该基因的cDNA序列全长为1842bp,包含一个1488bp完整的开放读码框,编码495个氨基酸残基,属于糖基水解酶第十七家族,为疏水性蛋白,含有信号肽。将ScBG1与其他植物种的β-1,3-葡聚糖酶基因进行序列比对,发现该基因具有保守和典型的葡聚糖酶结构域,碱基序列的保守性为45%~73%。研究结果为该基因的实验克隆奠定基础,也为甘蔗β-1,3-葡聚糖酶基因的分子功能研究提供参考。

关键词：甘蔗 β-1,3-葡聚糖酶基因电子克隆生物信息学

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