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功能化外泌体重编程免疫细胞与肿瘤细胞之间靶向识别

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中国生物工程杂志 2023年第10期43卷 1-9页

作者：项建叶邦策尹斌成华东理工大学生物反应器工程国家重点实验室上海200237 石河子大学化学化工学院石河子832003

目的:通过基因工程技术制备表面展示具有促进膜融合作用的水泡性口炎病毒糖蛋白G(vesicular stomatitis virus glycoprotein,VSVG)的外泌体VSVG-Exos,利用核酸探针介导DNA杂交链式反应在其膜表面修饰靶向树突状细胞间黏附分子-3-结合非... 详细信息

目的:通过基因工程技术制备表面展示具有促进膜融合作用的水泡性口炎病毒糖蛋白G(vesicular stomatitis virus glycoprotein,VSVG)的外泌体VSVG-Exos,利用核酸探针介导DNA杂交链式反应在其膜表面修饰靶向树突状细胞间黏附分子-3-结合非整合素DC-SIGN的核酸适配体,构建功能化外泌体,通过重编程肿瘤细胞与树突细胞之间的靶向识别过程增强相互作用。方法:以小鼠乳腺癌细胞4T1和小鼠树突状细胞DC2.4为研究对象,通过共聚焦成像和流式细胞术等实验证明VSVG-Exos以膜融合方式特异性结合4T1细胞,以及DC-SIGN适配体具有特异性靶向DC2.4细胞的能力。结果:功能化外泌体可以重编程修饰4T1细胞,增强与DC2.4细胞之间的靶向识别效应。结论:功能化外泌体有效地输送具有特定功能的分子到肿瘤细胞表面,对其进行重编程修饰,提高免疫细胞精准定位和高效攻击肿瘤细胞的能力,为靶向清除肿瘤细胞提供了新的思路和策略。

关键词：功能化外泌体靶向识别核酸适配体膜融合水泡性口炎病毒糖蛋白

Semibatch Emulsifier-free Emulsion Copolymerization of Methyl Methacrylate and n-Butyl Acrylate in the Presence of 2-Hydroxyethyl Methacrylate

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Chinese Chemical Letters 1998年第7期9卷 683-686页

作者： Tian Ying GUO Mou Dao SONG Qing Ye ZHOU bang Hua ZHANG jian Biao MA Bing Lin he (The State key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chendstry, Nankai University, Tianin, 300071) State Key Lab. Funct. Poly. M.A.S. Institute of Polymer Chemistry Nankai University Tianjin 300071 China

The semibatch emulsifier-free emulsion copolymerization of methyl methacrylate (MMA) and n-butyl acrylate (BA) in the presence of 2-hydroxyethyl methacrylate (heMA) initiated by K2S2O8 (PSP) was studied. The average particle diameter increases with an increase of total solids content, heMA content, PSP content, ionic strength of the system and monomer feed rate, and decreases with the monomer feed ratio from 3/1 (MMA/BA mole.) to 1/3. The stability of this reaction system is improved by adding heMA as nonionic comonomer. The high solids content (50%) latex with monodisperse particle can be obtained using this process.

关键词： emulsion copolymerization semibatch emulsifier-free MMA and BA latex stability

A Universal Formula for the Secondary Electron Yield of Metals at an Incident Angle of θ

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Chinese Physics Letters 2011年第9期28卷 253-256页

作者：谢爱根张健王铁邦 College of Math and Physics Nanjing University of Information Science and TechnologyNanjing 210044

Based on the main physical processes,we deduce the relationships among the incident energy Wp0 of the primary electron,the number of released secondary electrons(i.e.δ_(PEθ))per primary electron entering the metal at incident angleθand the angleθ*** addition,the relationship ofδPEθatθ=0°,i.e.δ_(PE0),with Wp0 is *** the experimental results,the relationship of the ratio atθ=0°,i.e.β_(0) which is the ratio of the average number of released secondary electrons generated by a single primary electron backscattered at the metal surface to that generated by a single primary electron entering the metal,with Wp0 is ***,the relationships among the ratioβθ,Wp0 andθare *** on the relationships among the secondary electron yield atθ(i.e.δθ),the yield atθ=0°(i.e.δ_(0)),the backscattering coefficient atθ(i.e.η_(θ)),the coefficient atθ=0°(i.e.η0),δ_(PEθ)andδ_(PE0),we deduce the universal formula forδ_(θ),δ_(0),η_(θ),η_(0),and W_(p0) for the primary electrons at an incident energy of 2–10 *** secondary electron yields calculated from the universal formula and the experimental yields of some metals are compared,and the results suggest that the proposed formula is universal for estimation of secondary electron yields atθ=0°−80°.

关键词： incident deduce coefficient

Characterization of molten 2LiF–BeF_2 salt impregnated into graphite matrix of fuel elements for thorium molten salt reactor

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Nuclear Science and Techniques 2019年第5期30卷 32-39页

作者： Hong-Xia Xu Jun Lin Ya-Juan Zhong Zhi-Yong Zhu Yu Chen jian-Dang Liu bang-Jiao Ye Center for Thorium Molten Salt Reactor System Shanghai Institute of Applied PhysicsChinese Academy of Sciences State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China USTC

The impregnation behavior of molten 2LiF–BeF_2(FLiBe) salt into a graphite matrix of fuel elements for a solid fuel thorium molten salt reactor(TMSR-SF) at pressures varying from 0.4 to 1.0 MPa was studied by mercury intrusion, molten salt impregnation, X-ray diffraction, and scanning electron microscopy *** was found that the entrance pore diameter of the graphite matrix is less than 1.0 μm and the contact angle is about 135°. The threshold impregnation pressure was found to be around 0.6 MPa experimentally, consistent with the predicted value of 0.57 MPa by the Washburn equation. With the increase of pressure from 0.6 to 1.0 MPa, the average weight gain of the matrix increased from 3.05 to 10.48%,corresponding to an impregnation volume increase from 2.74 to 9.40%. The diffraction patterns of FLiBe are found in matrices with high impregnation pressures(0.8 MPa and1.0 MPa). The FLiBe with sizes varying from tens of nanometers to a micrometer mainly occupies the open pores in the graphite matrix. The graphite matrix could inhibit the impregnation of the molten salt in the TMSR-SF with a maximum operation pressure of less than 0.5 MPa.

关键词： Keywords Molten salt reactor FLiBe Impregnation Graphite matrix

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柔性薄壁轴承的周期性冲击背景特性及其分离

振动工程学报 2022年第3期35卷 735-744页

作者：赵学智叶邦彦陈统坚华南理工大学机械与汽车工程学院广东广州510641

柔性薄壁轴承的内外圈是椭圆,这使得它和普通滚动轴承的振动特性完全不同。这种轴承旋转过程中椭圆长短轴会对轴承造成周期性冲击,这种正常的周期性冲击和轴承元件损伤引起的故障周期性冲击混合在一起,掩盖了故障周期性冲击。分析了这... 详细信息

柔性薄壁轴承的内外圈是椭圆,这使得它和普通滚动轴承的振动特性完全不同。这种轴承旋转过程中椭圆长短轴会对轴承造成周期性冲击,这种正常的周期性冲击和轴承元件损伤引起的故障周期性冲击混合在一起,掩盖了故障周期性冲击。分析了这种正常周期性冲击的频率分布特点,根据奇异值和频率的内在关系,提出利用奇异值分解(Singular Value Decomposition,SVD)来消除这种正常周期性冲击,选择正常周期性冲击的频率成分对应的奇异值进行SVD重构,可以准确地分离出这种正常冲击,从而消除其对故障周期性冲击的干扰。进而采用连续Morlet小波变换对消除了正常周期性冲击的柔性薄壁轴承振动信号进行故障冲击特征提取,选择峭度最大的尺度为故障特征尺度,清晰地提取到了柔性薄壁轴承的故障冲击特征,故障特征提取效果优于没有消除正常冲击时的故障特征提取效果。

关键词：故障诊断柔性薄壁轴承正常周期性冲击奇异值分解连续小波变换峭度

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工程化外泌体介导巨噬细胞清除肿瘤外泌体

中国生物工程杂志 2022年第6期42卷 1-11页

作者：王璐陈梦丽何芳项建尹斌成叶邦策华东理工大学生物反应器国家重点实验室上海200237 浙江工业大学药学院长三角绿色制药协同创新中心杭州310032 石河子大学化学化工学院石河子832003

目的:通过膜表面修饰改造技术构建工程化外泌体(engineered exosomes,enExos),并以此介导巨噬细胞特异性清除膜表面富含表皮生长因子受体(epidermal growth factor receptor,EGFR)的肿瘤外泌体。方法:利用表面展示技术获得膜表面展示趋... 详细信息

目的:通过膜表面修饰改造技术构建工程化外泌体(engineered exosomes,enExos),并以此介导巨噬细胞特异性清除膜表面富含表皮生长因子受体(epidermal growth factor receptor,EGFR)的肿瘤外泌体。方法:利用表面展示技术获得膜表面展示趋化因子(chemokine 8,CXCL8)的外泌体,同时在其磷脂双分子层上修饰EGFR核酸适配体制备工程化外泌体;纳米颗粒跟踪和纳米粒度电位分析enExos的尺寸、电位;CCK-8试剂盒检测细胞活力;透射电子显微镜观察enExos与高表达EGFR的肿瘤外泌体的特异性结合;荧光成像技术及流式细胞术分析探究enExos靶向趋化巨噬细胞吞噬高表达EGFR的肿瘤外泌体。结果:成功构建膜表面展示EGFR与CXCL8的工程化外泌体,enExos可以特异性识别并捕获高表达EGFR的肿瘤外泌体,同时利用其趋化因子CXCL8特异性靶向巨噬细胞膜表面趋化因子受体CXCR1/CXCR2,刺激巨噬细胞对肿瘤外泌体的捕获及清除。结论:工程化外泌体促进了特定肿瘤外泌体的清除,为后续深入研究工程化外泌体抑制癌症转移的作用奠定基础,并期望为癌症转移治疗提供新的研究方向。

关键词：工程化外泌体肿瘤外泌体表皮生长因子受体白细胞介素-8 吞噬