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Prevalence and haemato—biochemical profile of Anaplasma marginale infection in dairy animals of Punjab(India)

Asian Pacific Journal of Tropical Medicine 2013年第2期6卷 161-166页

作者： Ashuma Amrita Sharma Lachhman Das Singla Paramjit kaur Mandeep Singh Bal balwinder kaur batth Prayag Dutt Juyal Department of Veterinary Parasitology College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences UniversityLudhiana-141004India Animal Disease Research Centre Guru Angad Dev Veterinary and Animal Sciences UniversityLudhiana-141004India

Objective:To do the systematic comparison of prevalence of anaplasmosis by PCR and Giemsa stained thin blood smear(GSTBS) based parasitological assays in dairy cattle of Punjab,which has not been reported *** analyse the haematobiochemical alterations in infected animals to arrive at the conclusion regarding the pathogenicity induced by Anaplasma marginale (***) in latent and patent ***:Study was conducted on 320 animals (236 cows,62 calves and 22 buffaloes) of Punjab,*** on genome of *** was performed by targeting msp1βgene using specific primers BAP-2/AL34S,amplifies products of size 407 *** based data on the characteristics of the infected animals and management strategies of the farm were collected and ***:Higher prevalence and more significant association was observed in the PCR based molecular diagnosis(P=0.00012) as compared to that in GSTBS(P=0.028 8) based diagnosis with various regions under *** respect to the regions,highest prevalence was recorded in Ferozepur by PCR based diagnosis, while that in Jalandhar by GSTBS *** marked significant association of the PCR based diagnosis with the age of the animals under study(P=0.00013) was observed elucidating no inverse age resistance to *** in cow *** profile of infected animals revealed marked anemia,liver dysfunction and increase globulin concentrate indicating rise in immunoglobulin level to counteract ***:PCR is far more sensitive in detecting the disease even in latent infection which may act as nidus for spread of anaplasmosis to susceptible animals in endemic *** of anaemia and liver dysfunction were comparable both in patent as well as latent infection indicating pathogenicity of both.

关键词： Anaplasmosis Dairy animals Haemato-biochemical Prevalence Polymerase chain reaction

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