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Primarily screening and analyzing ESTs differentially expressed in rats' primary liver cancer

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Chinese Journal of Cancer Research 2013年第1期25卷 71-78页

作者： Dandan Liu Lijuan Zhi Mingxia Ma Dan Qiao Meijuan Wang Yawei Wang baijie Jin Anqi Li Guting Liu Yiqing Zhang Yanyan song Hongxu Zhang College of Life Science Henan Normal University Xinxiang University

Objective： To screen and analyze key express sequence tags （ESTs） which were differentially displayed in every period of SD rats＇ primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Methods： Using diethylnitrosamine （DENA） as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique （DDRT-PCR）. After the fragments were sequenced, bioinformatics were .used to analyze the results. Results： Twelve differentially expressed cDNA fragments were obtained. Nine fragments had the homology with known cDNA clones, especially EST-7 was similar to BN/SsNHsdMCW mitochondrion gene and the identity was 100% which suggested EST-7 may be the part of BN/SsNHsdMCW mitochondrion gene. In contrast, other three fragments （EST-1, EST-3 and EST-5） had extremely low identity to any genes registered in GENBANK databases. Conclusions： BN/SsNHsdMCW mitochondrion gene was expressed in different periods of hepatocarcinogenesis. Moreover, EST-I, EST-3 and EST-5 were suggested to contribute to the development of rat hepatocarcinogenesis, and thus may be candidates of new targets of oncogenes or cancer suppressor genes.

关键词： Animal models of primary liver cancer DDRT-PCR (differential display reverse transcription PCR) ESTs (express sequence tags) mitochondrion gene

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功能MRI研究特发性耳鸣机制进展

中国医学影像技术 2023年第1期39卷 113-116页

作者：李柏洁张雪婷付渊博刘慧林沈琳博于寒宋玉强首都医科大学附属北京中医医院针灸科(神经内科) 北京100010

特发性耳鸣是临床最常见耳鸣类型,指患者在无外界刺激情况下自觉耳内或颅内鸣响;因其发病原因及机制尚不明了,亦无特效治疗方法。功能MRI(fMRI)现已逐渐用于研究耳鸣患者大脑结构和功能变化,在研究耳鸣的中枢机制中发挥重要作用。本文就... 详细信息

特发性耳鸣是临床最常见耳鸣类型,指患者在无外界刺激情况下自觉耳内或颅内鸣响;因其发病原因及机制尚不明了,亦无特效治疗方法。功能MRI(fMRI)现已逐渐用于研究耳鸣患者大脑结构和功能变化,在研究耳鸣的中枢机制中发挥重要作用。本文就fMRI研究特发性耳鸣机制进展进行综述。

关键词：耳鸣磁共振成像中枢机制

基于VOSviewer和CiteSpace的针灸治疗功能性消化不良随机对照试验的可视化分析

同方期刊数据库博看期刊评论

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中国医药导报 2024年第26期21卷 161-167页

作者：徐怡宋玉强杜鑫袁芳王柏清李柏洁李青黛付渊博首都医科大学附属北京中医医院针灸科北京100010

目的基于VOSviewer、CiteSpace对针灸治疗功能性消化不良的随机对照试验(RCT)文献进行可视化分析,总结该领域的研究现状、热点及未来发展趋势。方法检索中国知网、万方数据知识服务平台、维普网、中国生物医学文献数据库、PubMed、Web o... 详细信息

目的基于VOSviewer、CiteSpace对针灸治疗功能性消化不良的随机对照试验(RCT)文献进行可视化分析,总结该领域的研究现状、热点及未来发展趋势。方法检索中国知网、万方数据知识服务平台、维普网、中国生物医学文献数据库、PubMed、Web of Science核心合集数据库中有关针灸治疗功能性消化不良的RCT文献,检索时限为2004年1月至2023年12月,利用VOSviewer、CiteSpace对该领域作者、机构、关键词进行可视化分析。结果最终纳入中文文献565篇、英文文献12篇。发文量排名前3位的作者为周利、刘存志、常小荣;发文最多的机构为湖北中医药大学;主要关键词为功能性消化不良、针刺、临床疗效;关键词聚类共10类;突现强度最大的关键词为辨证。结论针灸治疗功能性消化不良临床RCT研究目前正处于蓬勃发展阶段,不同疗法及穴位的选择是当前研究热点,研究趋势包括评价指标的选取及效应机制的探索。

关键词：针灸疗法功能性消化不良随机对照试验研究热点研究趋势 VOSviewer CiteSpace 可视化分析

Ecological Theory and Practice in Tourism Research in the New Era

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Journal of Resources and Ecology 2022年第1期13卷 142-160页

作者： SUN Yehong YAO Cancan CHEN Yuexin song Yuxin WANG Ying Tourism College of Beijing Union University Beijing 100101China Culture and Tourism Research Base of Beijing Union University Beijing 100101China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China College of Arts and Science of Beijing Union University Beijing 100083China Institute of Rural Development Zhejiang Academy of Agricultural SciencesHangzhou 310021China

With the development of tourism, the number of tourists and the scope of tourism activities are increasing, and the impact of tourism on the ecological environment is gradually revealed. The ecological environment is the foundation of sustainable tourism development. Some tourist destinations lack systematic and scientific evaluation and the proper planning of tourism resources, which makes it difficult for local areas to cope with the influx of a large number of people. In these destinations, the internal system is perturbed by a series of disturbances, which leads to bad effects on the local ecological environment. In addition to endangering the development of tourism, the quality of life of local residents is also threatened. Consequently, the issues between tourism and environment have become a hot topic in the field of tourism research. Through the collection of domestic and foreign tourism literature related to ecological theories since 1990 to 2020, the status of the application of various ecological theories in the field of tourism is revealed, such as life cycle theory, landscape ecology theory, carrying capacity theory, biodiversity, ecological niche theory and ecological footprint theory. This analysis found that the application of these theories to tourism mainly focuses on empirical research, and there are few studies on the basic theories related to it. In the empirical research, innovation of the research methods is basically absent. Therefore, future research should pay more attention to the theoretical research of ecological theory in the field of tourism,and combine modern science and technology in the practical application to strengthen the innovation of research methods. In addition, the prospect of applying ecological theories in future tourism research is discussed.

关键词： tourism research ecological theory research progress

Sustainable or Not?Tourism Development in Agricultural Heritage Sites

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Journal of Resources and Ecology 2021年第4期12卷 543-554页

作者： SUN Yehong song Yuxin CHEN Yuexin YAO Cancan LI Wenhua Tourism College of Beijing Union University Beijing 100101China Culture and Tourism Research Base of Beijing Union University Beijing 100101China Institute of Geographic Science and Natural Resources Research Chinese Academy of SciencesBeijing 100101China College of Arts and Science of Beijing Union University Beijing 100083China

Tourism is often considered as one of the dynamic conservation and adaptive management approaches in Agricultural Heritage *** has been over 15 years since the GIAHS programme was initiated in China,and tourism developed quickly in the Agricultural Heritage Sites,to some extent because many researchers consider tourism as a significant engine of the local ***,this is contrary to the original intention of agricultural heritage tourism as it was proposed in the first ***,there are some overt problems during the tourism development process,which are mainly as follows:Some threats to Agricultural Heritage Systems are ubiquitous;The tourism development mode in Agricultural Heritage Sites is questionable;Community involvement is difficult to implement;And the negative environmental impacts are easy to *** the context of global development,the UNWTO sustainable tourism criteria provide some guidance for agricultural heritage *** on the Global Sustainable Tourism Criteria,combined with previous survey experiences and related researches,this paper analyzes the tourism sustainability of all the 15 GIAHS sites in China,and explores the current sustainable tourism development *** this basis,an agricultural heritage sustainable tourism development framework was built in an attempt to find the road to sustainability for agricultural heritage *** framework in the global and local contexts is trying to connect all the important elements related to agricultural heritage tourism according to the UNWTO sustainable tourism criteria.

关键词： sustainable tourism Agricultural Heritage Systems tourism development

地下水流数值模拟在白界水源地开采方案设计中的应用

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广东化工 2015年第9期42卷 168-170页

作者：宋宝德广东省环境科学研究院广东广州510045

在详细分析白界水源地水文地质条件的基础上,建立了计算区的水文地质概念模型及数值模型,并应用Visual MODFLOW软件对模型进行了求解。通过模型识别及验证表明模型可以用来描述地下水流特征。根据预设的开采方案,通过对预测结果的分析... 详细信息

在详细分析白界水源地水文地质条件的基础上,建立了计算区的水文地质概念模型及数值模型,并应用Visual MODFLOW软件对模型进行了求解。通过模型识别及验证表明模型可以用来描述地下水流特征。根据预设的开采方案,通过对预测结果的分析选出了方案4为最优开采方案,其包含49口抽水井,在预定分布模式下,抽水量为163.27 m3/d。

关键词：数值模拟地下水最优开采方案白界水源地

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猪八戒“食”“色”特性缘由探析

柳州师专学报 2009年第1期24卷 31-33页

作者：宋兴昌孟智西藏民族学院人文学院陕西咸阳712000

《西游记》中,猪八戒的"食"、"色"本性表现得淋漓尽致,猪八戒这一艺术形象也因此而深入人心。但是为何猪八戒就应该被赋予"食"、"色"特性呢?为何必须是被赋予了"食"、"色"特性的猪八戒才应该出现在取经队伍之中呢?笔者从中国传统文化中... 详细信息

《西游记》中,猪八戒的"食"、"色"本性表现得淋漓尽致,猪八戒这一艺术形象也因此而深入人心。但是为何猪八戒就应该被赋予"食"、"色"特性呢?为何必须是被赋予了"食"、"色"特性的猪八戒才应该出现在取经队伍之中呢?笔者从中国传统文化中猪"意象"的阐释出发,结合《西游记》文本的成文背景,探析与解答这两个问题。

关键词：猪八戒食色本性意象

白竭散对肛瘘术后创面愈合及瘢痕形成的影响观察

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中国肛肠病杂志 2019年第9期39卷 24-26页

作者：张宏吴松王建民安徽六安世立医院安徽六安237000 安徽中医药大学第一附属医院安徽合肥230000

为研究白竭散外敷对肛瘘术后创面愈合及瘢痕形成的影响,将90例肛瘘手术患者按入院ID号分为治疗组和对照组。治疗组45例采用白竭散创面换药,对照组45例采用重组牛碱性成纤维细胞生长因子创面换药。比较2组术后瘢痕大小、并发症、创面愈... 详细信息

为研究白竭散外敷对肛瘘术后创面愈合及瘢痕形成的影响,将90例肛瘘手术患者按入院ID号分为治疗组和对照组。治疗组45例采用白竭散创面换药,对照组45例采用重组牛碱性成纤维细胞生长因子创面换药。比较2组术后瘢痕大小、并发症、创面愈合时间及愈合率。结果显示,治疗组创面愈合时间(15.63±3.27)d明显短于对照组(21.82±4.03)d;术后4d、9d及14d创面愈合率明显高于对照组;瘢痕形成状况明显优于对照组;并发症发生率(2.22%)明显低于对照组(13.33%),2组比较差异均有统计学意义(P<0.05)。结果表明,白竭散外敷能明显缩短创面愈合时间,改善瘢痕形成状况,减少并发症发生,值得临床推广应用。

关键词：肛瘘白竭散创面愈合瘢痕

不同品种桂花转录组分析及桂花精油成分差异的初步探讨

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天然产物研究与开发 2016年第4期28卷 529-535页

作者：张雪松裴建军赵林果汤锋房仙颖解静聪南京林业大学南京210037 江苏农林职业技术学院句容212400 国际竹藤中心北京100714

为丰富桂花转录组数据信息,阐明不同品种桂花精油合成的分子差异,本研究选择三种不同品种桂花,通过Illumina Hiseq2000平台测序,总计产出14.4G数据,三个品种的桂花样品共获得70029个Unigene序列,平均长度762 nt,N50达到1183 nt。通过Uni... 详细信息

为丰富桂花转录组数据信息,阐明不同品种桂花精油合成的分子差异,本研究选择三种不同品种桂花,通过Illumina Hiseq2000平台测序,总计产出14.4G数据,三个品种的桂花样品共获得70029个Unigene序列,平均长度762 nt,N50达到1183 nt。通过Unigene的表达差异分析与通路富集分析,发现与桂花精油物质合成途径有关的差异基因主要注释在二萜合成、萜类骨干化合物合成、单萜合成、倍半萜与三萜合成、苯丙烷类合成、苯丙酸类代谢、亚麻酸代谢以及亚油酸代谢8个通路中。而三种桂花差异基因均显著富集的通路则注释在苯丙烷类合成、苯丙酸类代谢、二萜合成以及萜类骨干化合物合成通路,共获得650个编码精油物质合成途径中54个差异酶的差异基因。本研究建立了以金球桂、白洁和日香桂为代表的桂花转录组数据库。通过转录组分析,确定了不同品种桂花精油物质合成差异的表达途径,初步分析了转录组数据库中与精油物质合成途径相关的差异基因。本研究将为桂花精油成分代谢调控及体外表达提供依据。

关键词：桂花精油合成转录组分析 RNA-seq