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Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus

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Asian Pacific Journal of Tropical Medicine 2012年第5期5卷 355-357页

作者： Jahangir Abdi bahram kazemi Mohammad Hasan Karimfar Mohammad Bagher Rokni Department of Medical Parasitology and Mycology School of ParamedicineIlam University of Medical SciencesHornIran Cellular and Molecular Biology Research Centre Shahid Reheshti University(Medical Campus)Tehran.Iran Department of Medical Parasitology and Mycology School of ParamedicineIlam University of Medical SciencesIlam.Iran Department of Medical Parasitology and Mycology School of Public HealthTehran University of Medical SciencesTehranIran

Objective:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus(***) and measuring polyclonal antibody and humoral immune response using ELISA and gel ***:Two mentioned antigens were cloned and expressed in expression vector and purified by affinity *** young rabbits were selected and challenged intradermallt with yielded recombinant ***’ sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last ***:The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post ***: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.

关键词： Cystic echinococcosis Hydatidosis Polyclonal antibody

First molecular identification of Leishmania species in a new endemic area of cutaneous leishmaniasis in Lorestan,Iran

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Asian Pacific Journal of Tropical Medicine 2013年第9期6卷 713-717页

作者： Farnaz Kheirandish Ali Chegeni Sharafi bahram kazemi Mojgan Bandehpour Mohamad javad Tarahi Ali Khamesipour Razi Herbal Medicines Research Center Department of Parasitology and MycologyLorestan University of Medical Sciences Department of Communicable Disease Control and Prevention Deputy of HealthLorestan University of Medical Sciences Cellular and Molecular Biology Research Center Shahid Beheshti University Department of Parasitology and Mycology School of MedicineShahid Beheshti University of Medical Sciences Department of Biotechnology School of MedicineShahid Beheshti University of Medical Sciences Department of Epidemiology School of Public HealthTehran University of Medical.Sciences Center for Research and Training in Skin Disease and Leprosy Tehran University of Medical Sciences

Objective:To identify Leishman{u using ***:This studs was conducted from April2009 to March 2011 in order to identify Leishmania species in a new endemic area of CL in *** were taken from 62 patients that referred to the health centers in different cities of Lorestan province,the presence of Leishmcania was confirmed using direct smear and then grown in NNN media and mass cultured in RPM!1640 medium supplemented with 10%heat-inactivated fetal bovine *** was extracted from cultured promastigotes and used in ***:45(72.6%)samples out of 62 showed a hand in the range of 485 hp and 17(27.4%)with a hand in the range of 626 hp which were similar to standard strains of Leichmania tropica(*** and Leishnrania major(***),respectively.50(65.80%)of samples were collected from people with no history of travel in at least a year prior to the onset which shows that indigenous source of ***:Since the vector and reservoir of the two species are *** precise and extensive control and prevention methods should be designed and earned out.

关键词： Cutaneous leishmaniasis Leishmania tropica Leishmania major PCR Lorestan Iran

Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells(PBMCs)expressing ectopic hCG,and hCG-activated PBMCs

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Asian pacific Journal of Reproduction 2023年第2期12卷 90-96页

作者： Delsuz Rezaee Mojgan Bandehpour bahram kazemi Saiyad Bastaminejad Sajad Najafi Mohammad Salehi School of Allied Medical Sciences Ilam University of Medical SciencesIlamIran Department of Medical Biotechnology School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran Cellular and Molecular Biology Research Center Shahid Beheshti University of Medical SciencesTehranIran

Objective:To compare the effect of human chorionic gonadotropin(hCG)-producing peripheral blood mononuclear cells(PBMCs)and PBMCs activated by hCG in vitro and expressions of related immune genes in mouse ***:hCG-producing PBMCs(transfected PBMC)and PBMCs activated by hCG in vitro were introduced into isolated mouse endometrial cells,while cell cultures were divided into four groups:the control,PBMC,transfected,and activated PBMC *** expression of studied genes(IL-1β,IL-6,Lif,and Vegf)was evaluated and blastocyst attachment on the cocultured cells(isolated endometrial cells and PBMC cells)was monitored in all four ***:Data showed that expression decreased in the PBMC group compared to the treated PBMC(transfected and activated PBMCs)and increased in transfected PBMC compared to the activated *** and migration of blastocysts were dramatically enhanced in the transfected PBMC group compared to the activated PBMC group(P<0.05).Conclusions:Use of hCG-producing PBMCs(transfected PBMC)has more influence on endometrial receptivity.

关键词： Peripheral blood mononuclear cells hCG Embryo attachment Immune response Immune cells In vitro Expression gene

Cloning and expression of Neisseria meningitides luxS gene

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Asian Pacific Journal of Tropical Medicine 2008年第2期1卷 16-20页

作者： bahram kazemi Nazila Baghalzadeh-Mohammadi Reza Hadavi Mojgan Bandehpour Elham Ghayour Majid Moghbeli Kazem Parivar Cellular and Molecular Biology Research Center Shahid Beheshti UniversityM.C.TehranI.R.Iran Parasitology Department Shahid Beheshti UniversityM.C.TehranI.R.Iran TehranI.R.Iran Islamic Azad University Jahrom BranchJahromI.R.Iran

Neisseria meningitides is a Gram-negative bacterium which is an important causative agent of septicemia and *** pathogenic bacteria contain luxS,which is required for autoinducer-2 *** meningitides contains a functional copy of luxS that is necessary for full meningococcal *** meningitides DNA was extracted and its luxS gene was amplified by nested *** product was purified and cloned in to pQE-30 expression *** plasmid was transformed and mass *** was expressed in *** and confirmed by western blot *** this study,*** luxS was amplified, cloned and expressed *** sequencing of PCR product confirmed that amplified gene was luxS. Gene was expressed and observed in *** was reacted by his tag monoclonal antibody through western blot analysis.

关键词： Neisseria meningitides luxS auto inducer 2 cloning

Influence of random heterogeneity of shear wave velocity on sliding mass response and seismic deformations of earth slopes

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Earthquake Engineering and Engineering Vibration 2020年第2期19卷 269-287页

作者： Pourya kazemi Esfeh bahram Nadi Nicholas Fantuzzi Department of Civil ChemicalEnvironmentaland Materials EngineeringUniversity of BolognaItaly Department of Civil Engineering Azad University of NajafabadIran

Soil shear wave velocity has been recognized as a governing parameter in the assessment of the seismic response of *** spatial variability of soil shear wave velocity can influence the seismic response of sliding mass and seismic ***,most analyses of sliding mass response have been carried out by deterministic *** paper stochastically investigates the effect of random heterogeneity of shear wave velocity of soil on the dynamic response of sliding mass using the correlation matrix decomposition method and Monte Carlo simulation(MCS).The software FLAC 7.0 along with a Matlab code has been utilized for this *** influence of statistical parameters on the seismic response of sliding mass and seismic displacements in earth slopes with different inclinations and stiffnesses subject to various earthquake shakings was *** results indicated that,in general,the random heterogeneity of soil shear modulus can have a notable impact on the sliding mass response and that neglecting this phenomenon could lead to underestimation of sliding deformations.

关键词： random heterogeneity random fields Monte Carlo simulation sliding mass response seismic deformations