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Ochratoxigenic Black Aspergilli Isolated from Dried Agricultural Products in Yogyakarta, Indonesia

Journal of Food Science and Engineering 2013年第9期3卷 472-480页

作者： Apriyanto Dwi Nugroho Francis Maria Constance Sigit Setyabudi baharuddin salleh Endang Sutriswati Rahayu Faculty of Agricultural Technology Universitas Gadjah Mada Yogyakarta Indonesia School of Biological Science Universiti Sains Malaysia Penang Malaysia

Black aspergilli, the potential ochratoxin A （OTA） producers, were predominant fungi in fermented cocoa bean, coffee bean and dried cassava in Yogyakarta. Identification of black aspergilli at species level will be useful to make clear link between OTA contamination on food product and the toxin producer. The objective of this study was to identify the species of the black aspergilli producing OTA which contaminated dried agriculture products. In this study, 16 isolates were obtained, and four isolates among of them （YAC-9, YAK-6, YAK-12 and YAG-2） were found as OTA producing-strains, with the highest OTA found on Yeast Extract Sucrose （YES） solid medium of 57.68 ppb. Based on morphological characters, 16 isolates can be grouped into four species, after confirmation by molecular data based on PCR method, the groups were identified as A. carbonarius, A. niger, A. tubingensis and A. aculeatus. OTA producing-strains were identified as A. carbonarius and A. niger, meanwhile, A. tubingensis and A. aculeatus were found as non-OTA producing-strains.

关键词： Black aspergilli ochratoxin A (OTA) PCR.

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Synthesis, Crystal Structure and Antimicrobial Activity of (E)-ethyl-4-(2-oxoacenaphthylen-1(2<i>H</i>)-ylideneamino) benzoate

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Journal of Crystallization Process and Technology 2013年第2期3卷 69-73页

作者： Yi-Chen Chan Abdussalam Salhin Mohamed Ali Melati Khairuddean baharuddin salleh School of Biological Sciences Universiti Sains Malaysia Penang Malaysia School of Chemical Sciences Universiti Sains Malaysia Penang Malaysia

A Schiff base, (E)-ethyl-4-(2-oxoacenaphthylen-1(2H)-ylideneamino)benzoate, (E4AB) had been synthesized in good yield by the acid-catalyzed condensation reaction of acenaphthenequinone and ethyl-4-aminobenzoate in methanolic solution. The synthesized compound was elucidated by elemental analysis (CHN), FTIR, 1H-NMR, 13C-NMR and single crystal X-ray diffraction. E4AB crystallized in the monoclinic crystal system with space group P21/c, Z = 4, V = 1569.3(2) ?3 and unit cell parameters a = 9.1589(8) , b = 21.2003(17)?, c =8.4502(7) ?, β= 106.972(2)°. The crystal structure of the compound is stabilized by intermolecular C-H···O hydrogen bonds and weak intermolecular π···π interactions. The title compound had been tested for the antimicrobial activity against Bacillus subtilis (B. subtilis), Enterobacter and Fusarium oxysporum f. sp. Cubense (Foc) by disc-diffusion method. E4AB is relatively active against Foc which is a pathogen that cause Wilt disease (also well known as Panama disease) in banana plantation.

关键词： Schiff Base Acenaphthenequinone Ethyl-4-aminobenzoate Antimicrobial Panama Disease

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