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Open Journal of Inorganic Chemistry 2011年第3期1卷 39-46页

作者： bader Belhaj Ali Mohamed Salah Belkhiria Michel Giorgi Habib Nasri 不详

The bis(nitrito-N) cobalt(III) octaethylporphyrin derivative with the formula [K(18-C-6)(NO2)0.5(H2O)0.5]2 [CoIII(OEP)(NO2)2] (1) (OEP = octaethylporphyrin and 18-C-6 is the crown ether18-crown-6) has been synthesized and characterized by UV-vis, IR and proton NMR spectroscopy which indicate, inter alia, that the cobalt ion presents the oxidation state III. The synthesis utilizes 18-crown-6 to solubilize potassium nitrite in the synthetic procedure. This compound crystallizes in the triclinic system, with the centric space group P-1 and the following unit cell parameters: 11.6724(2) ?, b = 12.5715(2) ?, c = 13.4722(3) ?, α = 101.1290(6)°, β = 106.6498(6)°, γ= 109.1507(6)°, Z = 1 and V = 1696.74(5) ?3. The crystal structure has been solved and refined to R = 0.0499 and R(w) = 0.1319. The X-rays molecular structure of 1 shows that there is a relatively close interaction of both two NO2- axial ligands of the ion complex [CoIII(OEP)(NO2)2]- with the potassium atoms of the two counterions [K(18-C-6) (NO2)0.5(H2O)0.5]0.5+. The title compound exhibits one-dimension polymer-like structure where “monomers” are linked together through strong O(H2O)—H…O(nitrito-O) hydrogen bonds.

关键词： Cobalt(III) Porphyrin X-Rays Diffraction UV-Visible 1H NMR

高效抗逆转录病毒治疗的HIV/HBV重叠感染者耐药HBV毒株C区和P区基因序列分析

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浙江大学学报（医学版） 2003年第6期32卷 507-509页

作者：朱彪吴南屏 Armin bader Norbert Brockmeyer 浙江大学医学院附属第一医院传染病研究所浙江杭州310003 德国鲁尔大学约瑟夫艾滋病研究中心

目的 :进一步研究乙肝病毒 (HBV)耐高效抗逆转录疗法 (HAART)的耐药机制。方法 :用 HBV- DNA荧光定量试剂盒 ,定量检测 36例 HAART治疗的 HIV/ HBV重叠感染者中 HBV- DNA,用 HBV- DNA C区和 P区引物常规扩增 HBV- DNA C区和 P区产物 ,PC... 详细信息

目的 :进一步研究乙肝病毒 (HBV)耐高效抗逆转录疗法 (HAART)的耐药机制。方法 :用 HBV- DNA荧光定量试剂盒 ,定量检测 36例 HAART治疗的 HIV/ HBV重叠感染者中 HBV- DNA,用 HBV- DNA C区和 P区引物常规扩增 HBV- DNA C区和 P区产物 ,PCR产物纯化后测序 ,用 BL AST软件分析序列 ,与国际 HBV基因库对比。结果 :36例 HAART治疗的 HIV/ HBV重叠感染者中 ,HBV- DNA阳性 4例 (11.1% ) ,血清中 HBV- DNA拷贝数 3例在 10 4copies/ ml级 ,1例在 10 7copies/ ml级。对 1例 HBV- DNA在 10 7copies/ ml级标本进行 HBVDNA C区和 P区序列测定 ,用 BL AST软件与国际基因库对比 ,结果 ,C区 nt 2 4 12位 T→ C,nt 2 4 13位 T→ C,P区 nt 74 1位A→ G(YMDD→ YVDD)。结论 :HAART治疗的 HIV/ HBV重叠感染者 ,HBV耐药可能与 HBV- DNA C区和 P区联合变异有关。

关键词：人类免疫缺陷病毒逆转录病毒科/药物作用肝炎病毒,乙型重叠感染序列分析 DNA 高效抗逆转录疗法耐药

Vulnerability of Sunflower Germination and Metal Translocation under Heavy Metals Contamination

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American Journal of Plant Sciences 2019年第5期10卷 738-751页

作者： Rumana Sadiq Nazimah Maqbool bader-Un-Nisa &ensp Kauser Parveen Mumtaz Hussain Faculty of Science and Technology Government College Women University Faisalabad Pakistan Department of Botany University of Sargodha Lyallpur Campus Faisalabad Pakistan Department of Botany University of Agriculture Faisalabad Pakistan

The germination and metal translocation ability of two sunflower seedlings were compared to identify the cultivar differences towards metal contamination at juvenile stage. The heavy metal treatments include: 0, 50, 100, 150 and 200 mM Ni, Cd and Pb applied in sand filled pots to Hysun-33 and FH-533 sunflower. The highest germination percentage (79%) and vigor index were recorded for Hysun-33 with no heavy metal treatment. Pb and Cd treatments reduced the growth attributes of 20 days old seedlings of both the cultivars. The Ni translocation effectively enhanced the shoot and root biomass of Hysun-33. The high concentration of 150 and 200 mM Cd and Pb drastically reduced Mn and K contents, vigor, length and biomass of two sunflower cultivars. Among three of the heavy metals, Cd was found more toxic than Pb and Ni. Roots of 20 days old seedlings of Hysun-33 were able to hold more Cd metal and stop its translocation to epigenous parts. Although 150 and 200 mM Ni effects the germination and vigor of sunflower cultivars more than 50 and 100 mM Ni, it is found less toxic in comparison to Cd and Pb. The Cd accumulation in roots suggests that it is physiologically most active sink for Cd metal while epigenous parts of sunflower cultivars are sink for Pb and Ni metal as shoot of sunflower cultivars accumulates high contents of Pb and Ni.

关键词： Translocation Germination Heavy Metals Contamination Sunflower

HIV感染者高效抗逆转录病毒治疗失败后的耐药研究

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浙江大学学报（医学版） 2003年第2期32卷 104-106页

作者：邹薇吴南屏 Armin bader Norbert Brockmeyer 浙江大学附属第一医院传染病研究所浙江杭州310003 德国鲁尔大学约瑟夫艾滋病研究中心

目的 :研究抗 - HIV治疗失败患者体内 HIV RNA和 DNA的基因变异情况 ,探讨其耐药机理从而指导临床用药 ,并对现有耐药数据库进行补充。方法 :对德国鲁尔大学艾滋病中心 10例抗 HIV耐药患者血浆中抽提HIV RNA和 DNA,分别对其逆转录酶区 (... 详细信息

目的 :研究抗 - HIV治疗失败患者体内 HIV RNA和 DNA的基因变异情况 ,探讨其耐药机理从而指导临床用药 ,并对现有耐药数据库进行补充。方法 :对德国鲁尔大学艾滋病中心 10例抗 HIV耐药患者血浆中抽提HIV RNA和 DNA,分别对其逆转录酶区 (RT)和蛋白酶区 (PI)进行 PCR扩增 ,产物回收后进行测序 ,得到的序列与国际标准株 HXB2 CG和 Standford大学耐药数据库比较 ,从而找出变异位点 ,并结合临床进行分析讨论。结果 :我们发现了一些能引起对某些药物产生相应耐药的有意义的突变位点 ,并与临床结果一致。我们还发现在一些患者中存在的突变 ,在 Standford大学耐药数据库中还未报道。结论 :HAART治疗失败者存在不同程度的逆转录酶区和蛋白酶区变异 ,逆转录酶区 V197I,蛋白酶区 K2 0 T,K2 0 I等变异可能与耐药有关。

关键词： HIV感染高效抗逆转录病毒治疗耐药机理失败基因变异艾滋病

Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment

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World Journal of Stem Cells 2023年第12期15卷 1093-1103页

作者： Ejlal Abu-El-Rub Fatimah Almahasneh Ramada R Khasawneh Ayman Alzu'bi Doaa Ghorab Rawan Almazari Huthaifa Magableh Ahmad Sanajleh Haitham Shlool Mohammad Mazari Noor S bader Joud Al-Momani Department of Basic Medical Sciences Faculty of MedicineYarmouk UniversityIrbid 21163Jordan Department of Pathology Faculty of MedicineMansoura UniversityMansoura 35516Egypt Department of Basic Medical Sciences Yarmouk UniversityIrbid 21163Jordan

BACKGROUND Mesenchymal stem cells(MSCs)are a type of stem cells that possess relevant regenerative abilities and can be used to treat many chronic *** mellitus(DM)is a frequently diagnosed chronic disease characterized by hyperglycemia which initiates many multisystem complications in the *** patients can benefit from MSCs transplantation to curb down the pathological consequences associated with hyperglycemia persistence and restore the function of damaged *** therapeutic outcomes are found to last for short period of time and ultimately these regenerative cells are eradicated and died in DM disease *** To investigate the impact of high glucose or hyperglycemia on the cellular and molecular characteristics of *** Human adipose tissue-derived MSCs(hAD-MSCs)were seeded in low(5.6 mmol/L of glucose)and high glucose(25 mmol/L of glucose)for 7 ***,viability,mitochondrial dynamics,and apoptosis were deplored using specific *** blotting was performed to measure the protein expression of phosphatidylinositol 3-kinase(PI3K),TSC1,and mammalian target of rapamycin(mTOR)in these *** hAD-MSCs cultured in high glucose for 7 d demonstrated marked decrease in their viability,as shown by a significant increase in lactate dehydrogenase(P<0.01)and a significant decrease in Trypan blue(P<0.05)in these cells compared to low glucose *** membrane potential,indicated by tetramethylrhodamine ethyl ester(TMRE)fluorescence intensity,and nicotinamide adenine dinucleotide(NAD+)/NADH ratio were significantly dropped(P<0.05 for TMRE and P<0.01 for NAD+/NADH)in high glucose exposed hAD-MSCs,indicating disturbed mitochondrial ***3K protein expression significantly decreased in high glucose culture MSCs(P<0.05 compared to low glucose)and it was coupled with significant upregulation in TSC1(P<0.05)and downregulation in mTOR protein expression(P<0.05).Mitochondrial complexes I,IV,and V were downregul

关键词： Mesenchymal stem cells High glucose Mitochondrial dynamics Apoptosis Poor survival Phosphatidylinositol 3-kinase/mammalian target of rapamycin pathway

Diagnosis and Damage Assessment of Weathered Quartzite Colossi of 18<sup>th</sup>Dynasty from Karnak Temple, Egypt

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Open Journal of Geology 2017年第1期7卷 51-68页

作者： Abd Elhakim A. El-Badry Abdelkareem E. Ahmed Nabil A. bader Ministry of State for the Antiquities Affairs Cairo Egypt Conservation Department Faculty of Archaeology South Valley University Qena Egypt

The Egyptian Pharaonic temples are traditionally made of different stones (limestone granite, diorite, or sandstone and quartzite) shaped into large heavy blocks or as Colossal statues. One of these is the colossi of Thutmose II and Amenhotep III at Karnak temple which made out of red quartzite from Gebel el-Ahmar, located at north-east of Cairo. Quartzite is one of the famous stones that were widely used during the heights of ancient Egyptian civilization. The Colossi of Thutmose II and Amenhotep III were subjected to many exogenous and endogenous deterioration factors which causes of a severe damage of the stone materials. In this study we documented the weathering of these quartzite colossal statues using field recording and laboratory analysis to evaluate their conservation state. Weathering and deterioration aspects noted through light optical microscope (LOM), polarizing microscope (PM) and scanning electron microscope (SEM). The mineralogical characterization was performed using X-ray powder diffraction (XRD) and Energy dispersive analysis (EDX). The mineralogy and weathering study of quartzite on the Colossi of Thutmose II and Amenhotep III illustrate a succession of geochemical processes which have taken place at the colossi and it revealed that, they need for quick intervention.

关键词： Colossi Quartzite Karnak Temple Deterioration Conservation Concepts

HIV重叠肝炎病毒感染者血清IL-18和IL-10水平分析

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浙江大学学报（医学版） 2003年第2期32卷 94-96页

作者：吴南屏李丹 Armin bader Hoxtermann Stefan Norbert Brockmeyer 浙江大学医学院附属第一医院传染病研究所浙江杭州310003 德国鲁尔大学约瑟夫艾滋病研究中心

目的 :探讨细胞因子在 HIV- HBV/ HCV重叠感染中的意义。方法 :采用 ELISA法检测血清 IL- 18和 IL- 10的水平 ,荧光定量 PCR计数测定 HIV- RNA滴度 ,流式细胞仪检测 CD4 + T细胞。结果 :HIV感染后 ,血清 IL- 18显著高于正常对照 (P<0 .0... 详细信息

目的 :探讨细胞因子在 HIV- HBV/ HCV重叠感染中的意义。方法 :采用 ELISA法检测血清 IL- 18和 IL- 10的水平 ,荧光定量 PCR计数测定 HIV- RNA滴度 ,流式细胞仪检测 CD4 + T细胞。结果 :HIV感染后 ,血清 IL- 18显著高于正常对照 (P<0 .0 5 )。随着疾病的进展 ,IL- 18逐渐上升而 IL - 10逐渐下降。重叠感染组血清 IL - 18和 IL- 10显著高于单纯 HIV感染组。结论 :肝炎病毒感染是影响 HIV感染者血清细胞因子水平的一个协变量 ,上调 IL- 18和下调 IL - 10可能起到改善重叠感染的预后的作用。

关键词： HIV感染重叠感染肝炎病毒感染血清 IL-18 IL-10

Association of Human Herpesvirus 6 and 8 in Relapsing Remitting Multiple Sclerosis Type during Exacerbation

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Neuroscience & Medicine 2010年第1期1卷 33-37页

作者： Hassan Salama Mohamed El-Khateeb Rawya bader Mohamed Saleh Enas Hamad Department of Neurology Mansoura University ElJomhoria st Mansoura University Department of Microbiology and Immunology Mansoura University

Multiple Sclerosis (MS) is a chronic demyelinating disease of the CNS with assumed autoimmune etiology. Human herpes viruses have probable effects on relapsing-remitting MS pathogenesis presumably through molecular mimicry and/or bystander mechanisms. In this study we probed the possible contribution of the two herpes viruses, human herpesvirus 6 (HHV-6) and human herpesvirus 8 (HHV-8), in clinically definite multiple sclerosis (CDMS) pa-tients-relapsing remitting type (RRMS) during clinical exacerbations. All patients had no history of immune modulating or suppressing drugs intake in the last 6 months. The peripheral blood samples, from CDMS patients (n = 20) (13F/7M, age (y) = 30.3 ± 3.21) and other immune mediated neurological disorders (OIND) (11F/9M, age = 25.2 ± 12.1), (My-asthenia Gravis, Guillain Barré Syndrome, ischemic stroke in adolescent and young adult with no clear risk factors), as a control group, had been enrolled within 15 months (January-2007-- March -2008). We investigated the existence of specific deoxyribonucleic acid (DNA) sequences belonging to HHV-6 and HHV-8, using polymerase chain reaction (PCR) in the isolated peripheral blood mononuclear cells (PBMCs) and in plasma. PCR demonstrated HHV-6 DNA in 7 cases (35%), HHV-8 sequences in only one cases (5%) in PBMCs from 20 relapsed CDMS patients;all HHV-6 posi-tive cases showed positive plasma results, while the blood samples from 20 OIND patients showed negative results ex-cept one case (5%) out of 9 cases of GBS was positive for HHV-8 in PBMCs. We consequently concluded that there is considerable evidence in this study that proposed the roles of HHV-6 and HHV-8 in MS pathogenesis and clinical ex-acerbation.

关键词： Multiple Sclerosis HHV6 HHV8 Antiviral Drugs

HIV感染对记忆型和处女型CD4^+T细胞上CD95的影响

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浙江大学学报（医学版） 2003年第2期32卷 90-93页

作者：吴南屏李丹 Armin bader Hoxtermann Stefan Norbert Brockmeyer 浙江大学医学院附属第一医院传染病研究所浙江杭州310003 德国鲁尔大学约瑟夫艾滋病研究中心

目的 :了解 CD95在 HIV感染者记忆型和处女型 CD4 + T细胞表面的表达情况 ,探讨 Fas/ CD95在 HIV感染中的作用。方法 :采用流式细胞检测仪 (FACS)对 HIV感染者外周血 CD4 + T细胞表面 CD95、CD4 5 RA、CD4 5 RO的表达进行分析 ,用 EL IS... 详细信息

目的 :了解 CD95在 HIV感染者记忆型和处女型 CD4 + T细胞表面的表达情况 ,探讨 Fas/ CD95在 HIV感染中的作用。方法 :采用流式细胞检测仪 (FACS)对 HIV感染者外周血 CD4 + T细胞表面 CD95、CD4 5 RA、CD4 5 RO的表达进行分析 ,用 EL ISA检测血清中 Fas水平。结果 :HIV感染后 ,血清 Fas水平随疾病的进展而逐渐升高 ,同时伴有处女型 T细胞表面 CD95表达增高以及记忆型 T细胞 CD95表达逐渐下降。结论 :Fas参与了 HIV的感染过程。研究 Fas和 CD95、CD4 5 RA、CD4 5 RO之间的关系 ,可以加深对 HIV致病机制的认识 ,并可了解疾病的进展情况和指导治疗。

关键词： HIV感染记忆型处女型 CD4^-T细胞 CD95 艾滋病流式细胞检测

高效抗逆转录病毒治疗对T细胞亚群上第二受体影响的研究

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浙江大学学报（医学版） 2003年第2期32卷 97-100页

作者：李丹吴南屏 Armin bader Hoxtermann Stefan Norbert Brockmeyer 浙江大学医学院附属第一医院传染病研究所浙江杭州310003 德国鲁尔大学约瑟夫艾滋病研究中心

目的 :研究高效抗逆转录病毒治疗 (HAART)后 T细胞亚群上第二受体的变化及其意义。方法 :采用流式细胞仪检测 CD4 + 、CD8+ 、CD4 + CD4 5 RA+ 以及 CD4 + CD4 5 RO+ 细胞表面 CCR5及 CXCR4的表达水平。结果 :HIV感染后 ,第二受体在 CD4... 详细信息

目的 :研究高效抗逆转录病毒治疗 (HAART)后 T细胞亚群上第二受体的变化及其意义。方法 :采用流式细胞仪检测 CD4 + 、CD8+ 、CD4 + CD4 5 RA+ 以及 CD4 + CD4 5 RO+ 细胞表面 CCR5及 CXCR4的表达水平。结果 :HIV感染后 ,第二受体在 CD4和 CD8以及记忆型和处女型 CD4 T细胞亚群上的表达水平 ,与正常对照没有显著性差异。 HAART治疗仅能在一定程度上改变细胞表面的第二受体水平。结论 :第二受体参与了 HIV的致病过程 ,B期之前开始抗病毒治疗将有较好的疗效。

关键词：高效抗逆转录病毒治疗 T细胞亚群第二受体 HIV 艾滋病