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In vitro germination and propagation of a threatened medicinal orchid,Cymbidium aloifolium(L.) *** artificial seed

Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 971-976页

作者： Shreeti Pradhan babulal tiruwa Bijay Raj Subedee Bijaya Pant Plant Biotechnology and Biochemistry Laboratory Central Department of BotanyTribhuvan University

Objective:To study the in vitro germination and plantlet regeneration from artificial seeds of Cymbidium aloifolium(***),a highly threatened medicinal orchid of ***:Artificial seeds were produced in vitro by encapsulation of protocorms with 4%sodium alginate and 0.2 mol/L calcium chloride *** vitro germination and plantlet regeneration of the artificial seeds were tested by culturing them on different strength of Murashige and Skoog(MS) liquid media(0.25,0.5 and 1.0) and MS liquid medium supplemented with 0.5 mg/L benzyl amino purine and 0.5 mg/L naphthalene acetic *** produced artificial seeds were stored up to 28 d at 4 ℃.In order to check the viability,storage artificial seeds were treated with five different sterilization techniques(T_1 T_2,T_3 T_4,T_5) and inoculated on full strength(1.0) of MS liquid medium after each 7 d of interval upto28 th ***:The highest percentage of germination(100%) of artificial seed was obtained on quarter(0.25),half(0.5) and full(1.0) strength of MS liquid ***,full strength of MS liquid medium was more effective for earlier seedling development of *** seeds were successfully stored at 4 ℃ till 28 th *** T_1 and T_2showed 97.5%viability of storage artificial seeds and hence considered as the most effective sterilization techniques to recover the plant from storage artificial *** developed from artificial seeds were successfully acclimatized in potting mixture containing cocopeat,litter and sphagnum moss with 85%survival ***:The present study revealed that artificial seeds are the good alternative explants for in vitro mass propagation and short term conservation of ***.

关键词： Media Propagation Protocorms Encapsulation Artificial seed

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Efficient Plant Regeneration of Cymbidium aloifolium (L.) Sw., a Threatened Orchid of Nepal through Artificial Seed Technology

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American Journal of Plant Sciences 2016年第14期7卷 1964-1974页

作者： Shreeti Pradhan Babu Lal tiruwa Bijay Raj Subedee Bijaya Pant Plant Biotechnology and Biochemistry Laboratory Central Department of Botany Tribhuvan University Kirtipur Kathmandu Nepal Research Centre for Applied Science and Technology Tribhuvan University Kirtipur Kathmandu Nepal

Artificial seed technology is a method of considerable potential for mass propagation and conservation of rare, endangered and threatened species. In the present investigation, artificial seeds were obtained through encapsulation of three weeks old protocorm (3.0 ± 1.0 mm diameter) of Cymbidium aloifolium with calcium alginate. Artificial seeds were cultured on liquid Murashige and Skoog (MS) or Knudson (Kn C) medium at different strength (×1.0, 0.5, 0.25) and full strength (1.0) of both media supplemented with 0.5 mg/l 6-benzyl aminopurine (BAP) and 0.5 mg/l α-naphthalene acetic acid (NAA). Full strength of MS medium without plant growth regulators was found to be the most favourable condition for efficient plantlet regeneration of C. aloifolium (9.83 shoot and 2.66 roots per culture). The storage potential of artificial seed was tested at 4°C and room temperature (RT, 21°C ± 2°C) for up to 90 days on both media and found 83.33% viability at 4°C storage on MS media. Eighty five percent of plantlets regenerated from artificial seeds culture were successfully hardened in a potting mixture of cocopeat, clay and sphagnum moss (2:1:1). Hence, the methodology can be used for propagation and conservation of C. aloifolium through artificial seed system.

关键词： Artificial Seed Protocorms In Vitro Germination Encapsulation Media

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Diversity of Orchids in Terms of Their Distribution, Uses and Conservation in Annapurna Conservation Area of Nepal

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American Journal of Plant Sciences 2024年第6期15卷 422-440页

作者： Babu Lal tiruwa Basu Dev Neupane Rabin Kadariya Chiranjibi Prasad Pokheral Bijaya Pant National Trust for Nature Conservation (NTNC)-Annapurna Conservation Area Project (ACAP) Pokhara Nepal Central Department of Botany Tribhuvan University Kathmandu Nepal

The Annapurna Conservation Area (ACA), the first conservation area and the largest protected area (PA) in Nepal, is incredibly rich in biodiversity. Notwithstanding this, orchids in the ACA have not been explored enough yet thus making the need for ambitious research to be carried out. Previous study only included 81 species of orchids within ACA. This study aims to update the record of species and genera richness in the ACA. In total 198 species of orchids, belonging to 67 genera (40% and 62% of the total recorded orchid species and genera in Nepal) has been recorded in ACA. This represents an increase of 144% in species and 56% in genera over the previous data. Out of the 198 species, 99 were epiphytes, 6 were holomycotrophic and 93 were terrestrial. Among the 67 genera, Bulbophyllum (17) species were dominant, followed by Dendrobium (16), Herminium (10), Coelogyne, Plantanthera (9 each), Eria, Habenaria, Oberonia (8 each), Calanthe (7), and Liparis (6). Fifty-six species were found to be ornamentally significant and 85 species medicinally significant.

关键词： Orchids Annapurna Conservation Area Diversity Distribution Uses

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