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In Situ Determination of Iron Distribution and Oxidation State in Broiler Duodenal Wall by Synchrotron Radiation μ-XRF and μ-XANES

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Journal of Northeast Agricultural University(English Edition) 2020年第2期27卷 73-80页

作者： Sun Rui-tao Liu Da-sen Zhang Mei-mei babarinde emmanuel Liu Hua-wei Zhang Peng Shi Ru-bin College of Animal Sciences and Technology Northeast Agricultural UniversityHarbin 150030China College of Science Northeast Agricultural UniversityHarbin 150030China College of Life Sciences Northeast Agricultural UniversityHarbin 150030China

Micro-X-ray fluorescence(μ-XRF)and micro-X-ray absorption near edge structure(μ-XANES)have not been widely used in animal *** situ determination of iron distribution and oxidation state in broiler duodenal wall was done byμ-XRF andμ-XANES *** newly hatched healthy(1-day-old)Arbor Acres commercial male broilers were used in this *** chicks were fed a corn-soybean meal basal diet(96.00 mg of Fe/kg diet)from the 1st day to the 21st day,but were fed a semi-purified diet(12.50 mg of Fe/kg diet)after 21 days to deplete the body Fe *** the 28th day,after an overnight fast,all the birds were randomly allotted to four different Fe sources[FeSO4,Fe-Gly(II),FeCl3 and Fe-Gly(Ⅲ)].Three points five mL nutrient solution was injected to a broiler’s duodenum by technology of in situ ligated intestinal loops and a perfusion group without Fe sources added to the media was designed to exclude the effect of endogenous *** incubated for 30 min,duodenal midpieces sections were collected for analyses byμ-XRF andμ-XANES *** was found that Fe concentration for ferrous Fe sources[FeSO4 and Fe-Gly(Ⅱ)]was significantly higher than that for ferric Fe sources[FeCl3 and Fe-Gly(Ⅲ)]in the duodenal wall,moreover,Fe-Gly(Ⅱ)group samples had the largest amount of Fe.μ-XANES spectra of Fe for the four sources were basically the same in the duodenal wall,which indicated that despite the ferric iron supplied to duodenum,ferrous iron could also be absorbed into the duodenal wall.

关键词： μ-XRF μ-XANES Fe source Fe concentration duodenal wall

Clinico-Pathological Study of Invasive Fungal Sinusitis in Ibadan, Nigeria: A Case Series

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Case Reports in Clinical Medicine 2020年第4期9卷 87-95页

作者： Oluyemi Fasina Adekunle Daniel Gabriel Olabiyi Ogun John A. babarinde Department of Ophthalmology University College Hospital University of Ibadan Ibadan Nigeria Department of Otorhinolaryngology University College Hospital University of Ibadan Ibadan Nigeria Pathology Department University College Hospital University of Ibadan Ibadan Nigeria

Background: Invasive fungal sinusitis is one of the less common forms of fungal infection more commonly described in North Africa and Asia. It affects healthy and immunologically competent individuals typically complicating chronic rhinosinusitis. Surgical debridement and adjuvant antifungal treatment are the mainstay of management of this condition. Objective: To describe the clinical presentation, pathological features and management of patients with invasive fungal sinusitis managed at a tertiary health facility, southwestern Nigeria. Method: Medical records of all patients with invasive fungal sinusitis managed between January 2009 and December 2018 were retrospectively reviewed. Results: Six patients with invasive fungal sinusitis were managed during the study period. All patients were immunocompetent and the mean age at presentation was 30.7 years ± 7.2 years. The average duration of symptoms prior to presentation was 18.5 months (Interquartile range, 67.5 months), and all six patients had orbital involvement and intracranial extension of the mass at presentation. They all had surgical debridement, and the specimens were sent for histological examination. Mycological studies carried out on two specimens isolated Aspergillus fumigatusas the fungal agent and all patients were started on post-operative oral antifungal chemotherapy. Five patients completed their chemotherapy and fared well with no recurrence while one patient had irregular and incomplete antifungal treatment, and developed recurrent infection before abandoning further treatment. Conclusion: Chronic invasive fungal sinusitis is an uncommon infection and fairly difficult to manage. However, timely diagnosis and combined surgical and medical treatment can give good outcomes in the patients.

关键词： Clinico-Pathological Features Ibadan Immunocompetent Invasive Fungal Sinusitis Nigeria

Comparative Study of Proximate,Chemical and Physicochemical Properties of Less Explored Tropical Leafy Vegetables

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Journal of Northeast Agricultural University(English Edition) 2018年第1期25卷 16-23页

作者： Grace Oluwakemi babarinde Victoria Funmilayo Abioye Odunayo Omobitan Kassim Raji Department of Food Science and Engineering Ladoke Akintola University of Technology

The list of underutilized green leafy vegetables is enormous: ranging from commonly consumed and underutilized vegetables. There are data on commonly consumed vegetables, but information is sparse on nutritional, chemical and anti-nutritional properties of less explored vegetables. This work aimed to evaluate some attributes of less explored or underutilized leafy vegetables. Some nutritional and anti-nutritional contents of 10 under-utilized leafy vegetables(Amaranthus spinosus Linnaeus, Basella alba Linnaeus, Corchorous tridens L., Cyrtosperma senegalense(Schott), Erigeron floribundus Sch, Hoslundia opposita Vahl, Lagenaria siceraria(Molina) Standl, Ocimum basilicum L., Solanum aethiopicum L. and Talinum portulacifolium(Forssk.) Asch. ex Schweinf) were investigated. Cyrtosperma senegalense had the highest protein value(5.93%). Hoslundia opposita had the highest fat content(0.54%). Amaranthus spinosus was high in β-carotene content(346.3 mg · 100 g-1); Cyrtosperma senegalense had the highest moisture content. A. spinosus had the highest ascorbic acid content(108.1 mg · 100 g-1). The highest tannin level(0.0057%) was found in C. senegalense and the lowest(0.0003%) was found in Solanum aethiopicum. Saponin was the highest in C. tridens(0.0052%) and the lowest in T. portulacifolium(0.00014%). C. senegalense and S. aethiopicum had their highest and the lowest phytate values, respectively. Although anti-nutrients could interfere with nutrient utilization, their values in these vegetables were not at toxic levels.

关键词： leafy vegetable nutritional attribute anti-nutritional property

Chemical Composition and Bioactivity of Lippia adoensis Hochst ***(Verneneaceae) Leaf Essential Oil Against Callosobruchus maculatus Fabricius(Coleoptera: Chrysomelidae)

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Journal of Northeast Agricultural University(English Edition) 2016年第4期23卷 8-14页

作者： babarinde Samuel Adelani Oladoye Sunday Olusegun Adesina Gabriel Olulakin Adebayo Moses Adeolu Department of Crop and Environmental Protection Ladoke Akintola University of Technology Department of Pure and Applied Chemistry Ladoke Akintola University of Technology Department of Crop Production and Soil Science Ladoke Akintola University of Technology

Essential oil （EO） of Nigeria-grown Lippia adoensis leaf was analyzed using gas chromatography mass spectrometry （GCMS） and its fumigant and repellent properties against Callosobruchus maculatus were evaluated. Sixteen compounds predominated by monoterpenes were identified. The major compounds were Eucalyptol （28.36%）, α-Terpineol （25.99%）, γ-Terpinene （15.24%）, α-Pinene （5.08%）, 1H-Cyclopropa[a]naphthalene （4.25%） and 1, 3, 6, 10-Dodeeatetraene （3.74%）. Percentage mortality due to fumigant toxicity was dose- and exposure period-dependent. One hour after treatment （HAT）, application of L. adoensis leaf EO at 107 μL· L^-1 air caused significantly （p〈0.05） higher mortality （50.00%） than 0.00% mortality observed at 0-53 μL· L^-1 air, but not significantly （p〉0.05） different from 22.50% observed in 80 μL· L^-1 air. At 3 HAT, application ofL. adoensis EO at 80 μL· L^-1 air caused significantly higher mortality （90.00 %） than mortality observed at 0 μL· L^-1 air. At 6 HAT, application of L. adoensis EO at 53-107 μL· L^-1 air caused significantly higher mortality （100.00 %） than that was observed in the control. The same trend was observed at 12 HAT where 100 % mortality observed in 27-107 μL· L^-1 air was significantly greater than 13.33 % observed in the control. At 3 HAT, percentage repellence was significantly （p〈0.05） affected by doses. Application of EO at 10-30 μL· cm^2 caused class V repellence （86.67%-100%） compared with the control which caused class I repellence （0-20%）.

关键词： Lippia adoensis Callosobruchus maculatus GCMS bioactivity repellence fumigant

Combination of Piper guineense Essential Oil with Cowpea Varietal Resistance in Control of Cowpea Seed Beetle, Callosobruchus maculatus Fabricius (Coleoptera： Chrysomelidae： Bruchinae)

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Journal of Northeast Agricultural University(English Edition) 2017年第3期24卷 10-18页

作者： Samuel Adelani babarinde Ebenezer Oluwaseun Esan Olagoke Zacchaeus Olatunde David Seye Ajayi Jesusegun Paul Olaniyi Department of Crop and Environmental Protection Ladoke Akintola University of TechnologyP.M.B.400Ogbomoso 210001Nigeria Maize Improvement Program for Southern Ecology Institute of Agricultural Research and TrainingMoor PlantationIbadan 200262Nigeria Department of Pure and Applied Chemistry Ladoke Akintola University of TechnologyP.M.B.400Ogbomoso 210001Nigeria

To reduce the risks associated with over-dependence on synthetic insecticides and ensure leguminous food security, an experiment was conducted to evaluate the potentials of integrating Piper guineense essential oil （PgEO） with cowpea varietal resistance in the control of cowpea seed beetle, Callosobruehus maculatus Fabricius. Four cowpea varieties （Sokoto, Drum, Oloyin and Gwallam） were studied. Data collected included oviposition, progeny emergence, Reproductive Efficiency （RE） and Percentage Seed Damage （PSD）. At first filial generation, numbers of eggs laid on Oloyin variety treated with 25 μL PgEO per 25 g cowpea seeds （2.15） were significantly （p〈0.05） lower than the values obtained in untreated cowpea （3.61） and hexane-treated seeds （3.07）. C. maculatus RE on Oloyin （33.73） was significantly （p〈0.05） lower than values obtained on other varieties （47.34-54.67） for seeds without PgEO; and were significantly greater than 0.00 observed on Oloyin seeds treated with 50 pL PgEO. PSD on Oloyin （6.63%） was significantly （p〈0.05） lower than the values obtained on Sokoto （10.04%） and Drum （9.79%） for seeds without PgEO, while there was no damage in Oloyin treated with 50 pL PgEO. Significantly （p〈0.05） lower F2 progeny emergence occurred on Oloyin （4.02） and Sokoto （4.71） compared with Drum （9.03） and Gwallam （6.07） from the untreated cowpea seeds. The results implied that application of PgEO to Oloyin gave better protection from infestation of C. maculatus than other varieties. Hence, combination of varietal resistance with PgEO for management ofbruchid could be an effective mean of improving cowpea food security.

关键词： cowpea variety Callosobruchus maculatus varietal resistance Piper guinensee filial generation integrated pest management

Efficacy of Botanical Insecticides in Control of Flea Beetle (Phyllotreta cruciferae) on Cabbage (Brassica oleracea Linn) on Field

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Journal of Northeast Agricultural University(English Edition) 2017年第3期24卷 30-36页

作者： Odewole Adeola Foluk babarinde Samuel Adelani Adebayo Timothy Abiodun Ojo Ayomide Roseline Department of Crop Protection and Environmental Protection Ladoke Akintola University of Technology P.M.B 4000 Ogbomoso Nigeria

An investigation of effect of Latana camara, Tephrosia vogelii and Zingiber officinale applied at 5%, 10% and 25% concentrations on flea beetle of cabbage was conducted on the Teaching and Research Farm of the Ladoke Akintola University of Technology Ogbomoso in the early seasons of 2011 and 2012. The experiment consisted of 11 treatments laid in a Randomized Complete Block Design （RCBD） with three replications. Data were subjected to analysis of variance and significant means were compared using the Least Significant Difference （LSD） at p〈0.05. All the tested plant extracts exhibited moderate efficacy in the control of flea beetle. The treatment plant extracts performed as well as synthetic insecticides （0.76±0.05 and 0.76±0.05, respectively）. It was discovered that 25% w/v of Z. officinale gave the best yield of 0.50 kg · hm-2. The result showed that extract of T. vogelii （0.71±0.00） and L. camara （0.96±0.23） could suppress flea beetle population at different hours of spraying, while Z. officinale showed an effect at 48 HAS （0.71±0.00） and 72 HAS （0.76±0.05） alter spraying.

关键词： Deltamethrin Latana camara Tephrosia vogelii Zingiber officinale insect pest