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Profile of Autoantibodies and Clinical Symptoms in Guinean Patients with Connective Tissue Diseases

Open Journal of Immunology 2024年第1期14卷 1-10页

作者： mariama Sadjo Diallo Doufin Traore Adame Diouf Djiba Kaba Issiaga Diallo Doudou Georges massar Niang Alimou Camara Oumar mouctar Diallo Boh Fanta Diane Abdoulaye Barry Ouo-Ouo Yaramon Kolie moustapha mbow Aly Badra Kamissoko Djibril Sylla babacar m.engue mohamed Cisse Faculté des Sciences et Techniques de la Santé Université Gamal Abdel Nasser de Conakry Conakry Guinée Laboratoire de Biologie moléculaire Nestor BANGOURA/Helene LABROUSSE H&#244pital de jour Donka CHU Donka Conakry Guinée Centre d’Excellence Africain Pour la Prévention et le Contrô le des Maladies Transmissibles Conakry Guinée Laboratoire d’Immunologie Université Cheikh Anta Diop Dakar Sénégal Service de Dermatologie-Vénérologie CHU Donka Conakry Guinée Service de Rhumatologie H&#244pital National Ignace Deen Conakry Guinée Service de médecine Interne CHU Donka Conakry Guinée

Connective tissue diseases (CTDs) are Autoimmune diseases (AIDs) characterized by the appearance of autoantibodies, which are diagnostic markers. Investigations of these autoantibodies play a major role in the management of several autoimmune diseases. The objective of this study was to describe the profile of anti-ENA antibodies according to the clinical symptoms of mixed CTDs in Conakry teaching Hospital. We performed a cross-sectional study during six months. A total of 20 patients was recruited and we measured antibodies using the ELISA technique. The mean age of our patients was 36.5 years, with a predominance of females. Cutaneous and rheumatological signs were the main clinical manifestations. SLP was the most frequent CTDs;the threshold of ENA antibodies positivity was higher in scleroderma with and SLP. Anti-ENA identification reveals the frequency of anti-SSA (83.33%), anti-U1RNP (66.66%) and anti-histone (50%) antibodies. Antinuclear antibodies (ANA) react with various components of the cell nucleus. Their detection is of major interest in the diagnosis of CTDs. Our results highlight the importance of determining the specificity of these antibodies to guide differential diagnosis.

关键词： Autoantibodies Extractible Nuclear Antigen (ENA) Connective Tissue Diseases ELISA

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Concurrent PIEZO1 activation and ATP2B4 blockade effectively reduce the risk of cerebral malaria and inhibit in vitro Plasmodium falciparum multiplication in red blood cells

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Genes & Diseases 2023年第6期10卷 2210-2214页

作者： mathieu Adjemout Bruno Pouvelle Fatou Thiam Alassane Thiam magali Torres Samia Nisar babacar m.engue Alioune Dieye Pascal Rihet Sandrine marquet Institut National de la SantéEt de la Recherche médicale(INSERm) UnitéMixte de Recherche(UMR)U1090Aix Marseille UniversitéTAGC Theories and Approaches of Genomic ComplexityInstitut MarMaRaMarseilleFrance Groupe de Recherche Biotechnologie Appliquée et Bioprocédés Environnementaux(GRBA-BE) Ecole Supérieure PolytechniqueUniversitéCheikh Anta Diop de DakarDakar BP5005Senegal Pole D’Immunophysiopathologie&maladies Infectieuses(ImI) Institut Pasteur de DakarDakar BP 220Senegal Service D’Immunologie UniversitéCheikh Anta Diop de DakarDakar BP5005Senegal Aix marseille University CNRSMarseille 13009France

malaria caused by the Plasmodium falciparum parasite is responsible for more than 240 million cases per year and killed 627,000 people in 2020,mostly African *** malaria parasite is transmitted by mosquitos belonging to the genus *** an asymptomatic liver stage,the parasite is released into the bloodstream to invade red blood cells(RBCs)and replicate *** erythrocytic phase is associated with a variety of clinical manifestations,including mild and severe *** malaria(Cm)is one of the most severe forms,characterized by the sequestration of parasitized RBCs in the small capillaries of the brain and the local development of cytokine-mediated *** variants in genes encoding proteins involved in red blood cell physiology are protective factors against severe malaria,as clearly demonstrated for the sickle cell variant of hemoglobin(HbS).

关键词： malaria falciparum Plasmodium

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Comparison of Two molecular Diagnostic Tests for COVID-19: Abbott RealTime SARS-CoV-2 and Allplex™2019-nCoV, in the Epidemic Context in Senegal

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American Journal of molecular Biology 2023年第1期13卷 46-56页

作者： babacar Faye Oulimata Gueye Doudou Georges massar Niang babacar m.engue Alioune Dièye Laboratory of molecular Biology Military Hospital of Ouakam Dakar Senegal AIDS Program of the Senegalese Armed Forces Military Hospital of Ouakam Dakar Senegal Service of molecular Biology Department of Pharmacy Faculty of Medicine Pharmacy and Odonto-Stomatology Cheikh Anta Diop University Dakar Senegal Service of Immunology Department of Pharmacy Faculty of Medicine Pharmacy and Odonto-Stomatology Cheikh Anta Diop University Dakar Senegal

Background: The persistence of the rapid spread of the COVID-19 pandemic is linked to the appearance of several variants of SARS-CoV2 with an impact on biological diagnosis, treatment and vaccination. The United States Food and Drug Administration (FDA) has granted several SARS-CoV-2 detection tests Emergency Use Authorization (EUA) for diagnosis and better epidemiological surveillance. Thus, multiple RT-PCR tests have been developed and brought to market in order to meet the urgent need for the diagnosis of COVID-19. However, comparative data between these tests in clinical laboratories are scarcely available to assess their performance. Objective: To compare two molecular methods for detecting SARS-CoV-2: the RT-PCR, Allplex™2019-nCoV tests on CFX96 Bio-Rad and the Abbott m2000sp/rt RealTime SARS-CoV-2. materials and methods: Nasopharyngeal and oropharyngeal swabs were taken from patients to diagnose SARS-CoV-2 infection. For each sample, we searched for the virus with two different RT-PCR tests: 1) first on Abbott m2000 SARS-CoV-2 targeting the N and RdRp genes, 2) then on Allplex™2019-nCoV Assay looking for the E, N and RdRp genes. Results: Percentages of the agreement were calculated. A total of 100 samples that tested negative and 90 positives on Abbott m2000 SARS-CoV-2 were retested on Allplex™2019-nCoV. Overall agreement was 74.74% on all samples. The specific agreement was 84% and 64.4% respectively for negative and positive samples with the RealTime SARS-CoV-2 test. A positive correlation (r2 = 0.63;p Conclusion: Our results showed good overall agreement between RT-PCR, Allplex™2019-nCoV and Abbott RealTime SARS-CoV-2 tests in the diagnosis of COVID-19. As the concordance is low for small viremias, the RT-PCR Allplex™2019-nCoV Assay would be better indicated during the acute and symptomatic phase of the disease.

关键词： SARS-CoV-2 COVID-19 Diagnostics RT-PCR Abbott RealTime Seegene-Allplex

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molecular Genotyping of Human Papillomavirus (HPV) in Hypopharyngeal Cancer

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American Journal of molecular Biology 2023年第4期13卷 227-240页

作者： babacar Faye Soukeynatou Diabira Omar Bassoum Doudou G. m. Niang malick Ndiaye Baye Karim Diallo Alioune Dièye Laboratory of molecular Biology Military Hospital of Ouakam (HMO) Dakar Senegal AIDS Program of the Senegalese Armed Forces Military Hospital of Ouakam (HMO) Dakar Senegal Service of molecular Biology Department of Pharmacy Faculty of Medicine Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar Dakar Senegal Public Health Unit Faculty of Medecine Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University of Dakar Dakar Senegal Service of Immunology Department of Pharmacy Faculty of Medicine Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar Dakar Senegal Service of ORL Fann Hospital Dakar Senegal

Background: In recent years, head and neck cancers have become common worldwide, ranking sixth in incidence. In 2007, in France the incidence increased by 14,697 including 11,158 among men, which places them in fourth place. The same year, 32,268 patients were hospitalized for this pathology, but 95% are associated with alcohol and tobacco poisoning. Few data exist on these cancers in Africa and Senegal. In recent years, many studies have hypothesized that about 25% of head and neck cancers are associated with high-risk oncogenic human papillomaviruses (HPV) whose role in cervical cancer was already widely established. Objective: To know the prevalence and genotypes of HPV in head and neck cancers, particularly hypopharyngeal cancer. material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the molecular Biology Unit of the Ouakam military hospital (HmO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNATm miniprep Plus kit” https://***/. molecular HPV detection and genotyping were performed by multiplex RT-PCR with the Seegene AnyplexTm II HPV28 kit Detection on a Biorad CFX96 automaton according to the manufacturer’s protocol for the simultaneous genotyping of 28 types of HPV including 19 at High Risk (HR) and 9 low risk (LR). Results: 156 patients were sampled, 61 Hypopharynx cancer biopsies and 95 healthy tissues. The median age of the general population was 36.5 years [12, 73];the median age of the population with hypopharyngeal cancer of 40 years. Of the general study population 24.36% (38/156) was infected with HPV. In populations with hypopharyngeal cancer, HPV prevalence was 19.67% (12/61), 17.84% (5/28) in men and 21.21% (7/33) in women. HPV6 was the most frequently encountered genotype in t

关键词： HPV Genotypes RT-PCR ENT Cancer Hypopharynx

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molecular Genotyping of Human Papillomaviruses (HPV) in HIV+ and HIV−Women in Senegal

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American Journal of molecular Biology 2022年第2期12卷 54-66页

作者： babacar Faye Serigne A. A. m. Gueye Jean A. D. Tine Hameth Sarr Alioune Dièye Laboratory of molecular Biology Military hospital of Ouakam (HMO) Dakar Senegal AIDS Program of the Senegalese Armed Forces Dakar Senegal Service of Immunology Department of Pharmacy Faculty of Medicine Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University Dakar Senegal Public Health Unit Faculty of Medecine Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University Dakar Senegal

Background: Different studies have demonstrated high prevalence of HPV infection and dysplastic lesions of the cervix in immunocompromised patient such as women living with HIV. Is this high prevalence due to a greater susceptibility to HPV infection, which is known to be frequent in its latent form in women? Objective: This study aims to identify HPV genotypes in HIV+ and HIV−women to understand HPV molecular epidemiology in Senegal. material and method: Endocervical samples from 331 HIV+ and HIV−women, sexually active, were collected. The molecular identification of the 28 genotypes studied (19 HPV-HR and 9 HPV-LR) was carried out after DNA extraction, by multiplex PCR with the Anyplex™II HPV28 detection kit from Seegene on CFX96™Bio-Rad machine. The comparisons were made by calculating the p-value and odds ratio with R Studio software (version 4.1.0). The results were considered significant if p Results: The general prevalence of HPV was significantly higher in HIV+ women with 78.95% vs 64.65% for HPV;72.18% vs 57.07% for HPV-HR;57.14% vs 34.34% for HPV-BR (p −women. Conclusion: Our results showed that the prevalence of HPV, HPV-HR and HPV-BR was significantly higher in HIV+ women. Non-vaccine genotypes were among the most found genotypes. Groups of HIV+ women aged between 35 and 50, married and using contraception were significantly more infected with HPV than the same groups of HIV-women.

关键词： HPV Prevalence Genotypes HIV PCR

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Relationship between Circulating Plasma Galectin-3 Levels and T-Cell Activation during Cervical Cancer Chemotherapy

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Open Journal of Immunology 2023年第1期13卷 14-31页

作者： Folly m. Gaba maïmouna Diop Doudou G. m. Niang Sidy Ka Doudou Diouf moussa Ndour Comlan J. G. montcho moustapha mbow babacar Faye Rokhaya N. Diallo maguette S. Niang Ahmadou Dem babacar m.engue Alioune Dieye Immunology Department Faculty of Medicine Pharmacy and Odontology University of Cheikh Anta Diop Dakar Senegal Immunology Laboratory Aristide Ledantec Hospital (HALD) Dakar Senegal Oncology Department Juliot Curie Institute Aristide Ledantec Hospital (HALD) Dakar Senegal Human Genetic Department Faculty of Medicine Pharmacy and Odontology University of Cheikh Anta Diop Dakar Senegal

Objective: Despite the existence of several therapeutic strategies, the management of cervical cancer remains challenging. Our region has very little data on the interaction between the immune system and the clinical response to chemotherapy. This work examines plasma levels of galectin-3 (Gal-3) and percentages of activated T cells in patients with cervical cancer treated with chemotherapy and investigates if there is a relationship between the rates of these two elements. methods: We compared data from 37 patients with cervical cancer undergoing chemotherapy and 42 controls with normal cervical cytology. Plasma Gal-3 concentrations were assessed by ELISA and expression of activation markers by T cells (CD69 and HLA-DR) was assessed by flow cytometry at three different time points during chemotherapy. Results: Our results showed that patients had a significantly higher concentration of Gal-3 compared to controls (4.025 vs. 1.340, p 0.001), similarly, they had a significantly high percentage of activated lymphocytes (2.610 vs. 0.731;p 0.0001). According to the response to treatment, patients with no response to treatment had a lower concentration of circulating Gal-3 but had approximately the same percentage of activated CD4 and CD8 lymphocytes as patients with a partial or total response. In addition, we found a positive correlation between the Gal-3 level and CD4 T cells expressing the activation marker CD69 (p 0.05;rho = 0.44). Conclusion: In conclusion, our results show that there would be a relationship between circulating galectin-3 and the percentage of peripheral CD4+CD69+ cells in cervical cancer.

关键词： Uterine Cervical Neoplasm Chemotherapy Galectin 3 T-Lymphocytes Activation

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Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis methods

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Food and Nutrition Sciences 2022年第11期13卷 950-961页

作者： Rokhaya Gueye Viviane Chatchueng Sandefo babacar Beye Elhadji Ousmane Faye Amadou Diop Serigne Omar Sarr Bara Ndiaye Yérim mbagnick Diop Laboratoire de Chimie Analytique et Bromatologie Faculté de Médecine de Pharmacie et d’Odontologie-Stomatologie Université Cheikh Anta DIOP Dakar Sénégal Laboratoire de mycotoxines Institut de Technologie Alimentaire Route des Pères Maristes Dakar Sénégal

Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.

关键词： Poultry Feed Aflatoxin High Performance Liquid Chromatography Thin-Layer Chromatography Dakar

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Prevalence and Resistance Profile of muenchen Cefotaximase (CTX-m) Group 1 Extended Spectrum Beta-Lactamase (ESBL)-Producing Uropathogenic Escherichia coli Strains in Dakar, Senegal

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Open Journal of medical microbiology 2023年第2期13卷 137-145页

作者： Abdoulaye Seck babacar Ndiaye Abdou Diop malick Ndao Cheikh Fall Assane Dieng Thierno Abdoulaye Diallo Chantal mahou Philippe Dubrous Laboratoire de Biologie médicale Institut Pasteurde Dakar Dakar Senegal Laboratoire de Bactériologie-Virologie Université Cheikh Anta Diop Dakar Senegal Laboratoire National de Santé Publique Thies Senegal Laboratoire de Bactériologie-Virologie H&#244pital Aristide Le Dantec Dakar Senegal

Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the muenchen Cefotaximase (CTX-m) described all over the world of which the most frequent is the CTX-m of group 1 particularly the CTX-m-15 variant. The objective of this study was to determine the prevalence of CTX-m group 1 ESBL-producing Escherichia coli strains and to test their antibiotics susceptibility profile. methodology: A retrospective cross-sectional descriptive study was conducted to detect ESBL-secreting Escherichia coli strains by the synergy test. Identification of CTX-m type ESBL from group 1 was performed using the NG-Test CTX-m rapid diagnostic test (NG-Biotech®). Antibiotic susceptibility profile was determined using CA-SFm/EUCAST guidelines 2019. Data entry and statistical analysis were performed with Excel version 2010 and SPSS 20.0 respectively. Results: Eighty-two ESBL-producing Escherichia coli strains were tested. A group 1 CTX-m ESBL was detected in 75.6% of the strains (n = 62). These strains were highly resistant to cefotaxim (100%), aztreonam (100%), ceftazidim (85.4%) and cefepim (66.1%). They were also resistant to quinolones, gentamycin and sulfadoxine-trimethoprim combination. However, these strains showed sensitivity to ertapenem (100%), cefoxitin (69.3%), tigecyclin (66%), and amikacin (66.1%). The combination of piperacillin and tazobactam was active on 30.6% of the strains against 6.4% for the combination of amoxicillin and clavulanic acid. Conclusion: The CTX-m type ESBL of group 1 was present in the majority of ESBL-producing Escherichia colis trains. Despite the production of this enzyme conferring resistance to most beta-lactam antibiotics, some antibiotics remain active to treat infections caused by these germs.

关键词： Escherichia coli ESBL CTX-m Group Prevalence Antibiotic Resistance

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Prevalence of Intestinal Parasitic Infection among Pre-School and School-Aged Children Attending to Fann University Hospital, Dakar: Results from Retrospective Analysis

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Advances in Infectious Diseases 2022年第4期12卷 847-860页

作者： Khadime Sylla Doudou Sow Souleye Lelo Cheikh B. Fall Isaac Akhenaton manga magatte Ndiaye babacar Faye Thérèse Dieng Roger Clement Tine Département de Parasitologie-mycologie Faculté de Médecine Pharmacie et Odontologie Université Cheikh Anta Diop Dakar Sénégal Laboratoire de Parasitologie-mycologie Centre National Hospitalier Universitaire Fann Dakar Sénégal Service de Parasitologie-mycologie UFR Sciences de la Santé Université Gaston Berger Saint-Louis Sénégal Laboratoire de Parasitologie-mycologie Centre National Hospitalier d’enfant Albert Royer Dakar Sénégal

Background: Intestinal parasitic infections represent a major public health problem worldwide. Pre-school and school children have a high risk of infection. The study aimed to determine the epidemiological aspects of intestinal parasitic infections in preschool and school-aged children in Dakar, Senegal. methods: A retrospective analysis of laboratory records from preschool and school-aged children referred to the Laboratory of Parasitology at the Fann University Hospital in Dakar, Senegal, was carried out. The analysis of the period from 2016 to 2020. Stool samples were examined for the presence of parasites using direct, formal-ether concentration method and the modified Ziehl Neelsen staining technique. Descriptive and analytic analysis was performed with Stata mP 16.1 software. Results: The overall prevalence of intestinal parasitic infection was 26.8% (429/1603). Blastocystis sp. (34%), Entamaeba coli (28%), Giardia intestinalis (9.6%), Endolimax nana (4.9%) and Ascaris lumbricoides (3.5%) were the common parasites found. Out of the 429 children positive for intestinal parasites, protozoa parasites represent 93.9%. Single infection and mixed infection represent 83.7% and 16.3%. The most prevalent associations were E. coli + Blastocystis sp. (6.3%), E. coli + E. nana (3.3%), and G. intestinalis + Blastocystis sp. (1.9%). Children between the ages of 5 - 10 years and those over 10 years had the most parasitic infections with 28.1% [aOR = 1.91;95% CI (1.25 - 2.89)] and 27.4% [aOR = 11.92 (1.07 - 3.07)]. According to the year of sample collection, the prevalence was higher in 2017, 32.6% [aOR = 1.49 (1.11 - 2.01]. The prevalence of intestinal parasitic infection was not significantly related to gender (p = 0.87), season (p = 0.85) and symptomatology (p = 0.19). The main clinical symptoms were dysenteric syndrome, dyspeptic disorders, constipation and abdominal pain. Intestinal parasite carriage in patients with acute diarrhea was 20.4%. In HIV positive with chronic

关键词： Intestinal Parasites Children Epidemiology Senegal

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Breakdown mechanic Location Problem

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Applied mathematics 2012年第5期3卷 467-472页

作者： Cheikh B. Djiba mamadou Balde babacar m. Ndiaye Roger m. Faye Diaraf Seck Laboratory of Information Processing ESP Cheikh Anta Diop University Dakar Senegal Laboratory of mathematics of Decision and Numerical Analysis Cheikh Anta Diop University Dakar Senegal Unité mixte Internationale UMMISCO Institut de Recherche pour le Développement Bondy France

This paper studies the problem of locating breakdown mechanic. We consider a public transport network in which it can provide buses failure. The objective is, taking into account the statistics of breakdowns registered on the network, to locate optimally breakdown mechanics so as to minimize the response time (to ensure the network coverage of break- down mechanics). In this work, we present a binary linear programming model for this location problem which provides assignments-locations of areas served. Once the location made, we discuss dynamic assignment of breakdown mechan- ics depending on their position in the network at a given time t. Numerical simulation results are presented based on real data of urban transportation society of Dakar Dem Dikk.

关键词： Optimization Location Assignment Urban Transportation Network

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