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Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri(Hemiptera:Psyllidae)

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Insect Science 2021年第3期28卷 718-734页

作者： Hai-zhong Yu Yu-Ling Huang Zhan-Jun Lu Qin Zhang Hua-Nan Su Yi-Ming Du Long Yi ba-lian zhong Ci-Xiang Chen College of Life Sciences Gannan Normal UniversityGanzhouJiangxiChina National Navel Orange Engineering Research Center GanzhouJiangxiChina China-USA Citrus Huanglongbing Joint Laboratory A Joint Laboratory of The University of Florida and Gannan Normal UniversityGanzhouJiangxiChina Fruit Bureau of Ganzhou GanzhouJiangxiChina

The Asian citrus psyllid,Diaphorina citri is the principal vector of huanglongbing,which transmits Candidatus Liberibacter *** is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and *** specific functions of this enzyme in *** have not been *** this study,three trehalase genes(DcTreJ-1,DcTrel-2,and DcTre2)were identified based on the *** genome *** analysis showed that DcTrel-1 and DcTrel-2 are related to soluble trehalase,whereas DcTre2 is associated with membrane-bound *** expression analysis indicated that DcTrel-1 and DcTrel-2 had the highest expression levels in the head and wing,respectively,and DcTre2 had high expression levels in the fat ***,DcTrel-1 and DcTrel-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone III,but DcTre2 was *** expression levels of DcTrel-1,DcTrel-2,and DcTre2 were significantly up-regulated,which resulted in high mortality after treatment with *** activities and glucose contents were downregulated,but the trehalose content increased after treatment with *** addition,the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with ***,silencing of DcTrel-1,DcTrel-2,and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of *** results indicate that *** trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of ***.

关键词： chitin metabolism Diaphorina citric RNA interference trehalase validamycin

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Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

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Insect Science 2021年第6期28卷 1690-1707页

作者： Hai-zhong Yu Yan-Xin Xie Jie Wang Ying Wang Yi-Min Du He-Gui Wang ba-lian zhong Bo Zhu Xiu-Dao Yu Zhan-Jun Lu College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina College of Life Science Anhui Agricultural UniversityHefeiChina

Validamycin has been widely used as a specific competitive inhibitor of *** our previous research,validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri,resulting in abnormal ***,the mechanism of validamycin's action on *** remains ***,using a comparative transcriptome analysis,464 differentially expressed genes(DEGs)in *** were identified after validamycin treatment.A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in“small molecule process”,“structural molecule ac-tivity”and“transition metal ion binding”.DEGs involved in chitin metabolism,cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain *** RNA interference of *** chitinase-like protein EN03 and *** cuticle protein 7 genes significantly affected *** ***,the recombinant chitinase-like protein EN03 exhibited a chitin-binding property,and an antimicrobial activity against bacillus *** study provides a first insight into the molecular changes in *** after exposure to validamycin and identifies two effective RNA interference targets for *** control.

关键词： chitin-binding Diaphorina citric RNA interference transcriptome sequencing validamycin

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Modeling and Simulation of Lithium Vacuum Evaporation Process Using COMSOL Multiphysics

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Journal of Thermal Science 2024年第1期33卷 86-100页

作者： lian Xuexin zhong Dawen Beijing Key Laboratory of Passive Nuclear Power Safety and Technology North China Electric Power UniversityBeijing 102206China

based on the COMSOL Multiphysics simulation software,this study carried out modeling and numerical simulation for the evaporation process of liquid metal lithium in the vacuum free molecular flow *** motion of lithium atoms in the evaporation process was analyzed through a succession of *** on the available experimental values of the saturated vapor pressure of liquid metal lithium,the relationship between saturated vapor pressure and temperature of liquid lithium in the range of 600 K-900 K was obtained.A two-dimensional symmetric model(3.5 mm×20 mm) was established to simulate the transient evaporation process of liquid lithium at wall temperatures of 750 K,780 K,800 K,810 K,825 K,and 850 K,*** effects of temperature,the evaporation coefficient,back pressure,and length-to-diameter ratio on the evaporation process were studied;the variation trends and reasons of the molecular flux and the pressure during the evaporation process were *** the same time,the evaporation process under variable wall temperature conditions was *** research made the evaporation process of liquid lithium in vacuum molecular flow clearer,and provided theoretical support for the space reactor and nuclear fusion related fields.

关键词： free molecular flow saturated vapor pressure vacuum evaporation liquid lithium metal

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Effect of harvest time on the chemical composition and antioxidant capacity of Gannan navel orange(Citrus sinensis L. Osbeck 'Newhall')juice

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Journal of Integrative Agriculture 2022年第1期21卷 261-272页

作者： ZHANG Jun ZHANG Jing-yi SHAN You-xia GUO Can HE lian ZHANG Lin-yan LING Wei lianG Yan zhong ba-lian National Engineering Research Center of Navel Orange Gannan Normal UniversityGanzhou 341000P.R.China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650P.R.China

The present study investigates the chemical composition and antioxidant capacity of juice from the Gannan navel orange, which is harvested at one-to two-week intervals during the ripening period. The total soluble solid(TSS), total polyphenol content(TPC), total flavonoid content(TFC), sucrose and hesperidin contents gradually increase with the ripening of the fruit, followed by slight declines at the late maturity stage. Contrary to these observations, the contents of titratable acid(TA), vitamin C(Vc), and limonin trend downward throughout the ripening period. However, the contents of fructose, glucose, and narirutin fluctuate throughout the harvest time. Three in vitro antioxidant assays consistently indicate that the harvest time exerts no significant influence(P>0.01) on the antioxidant capacity. Furthermore, principal component analysis(PCA) and Pearson’s correlation test are performed to provide an overview of the complete *** study provides valuable information for evaluating the fruit quality and determining when to harvest the fruit in order to meet the preferences of consumers. Meanwhile, our observations suggest that the fruits subjected to juice processing should be harvested at the late maturity stage to alleviate the "delayed bitterness" problem without compromising the antioxidant capacity and the flavonoid content in the juice.

关键词： navel orange antioxidant harvest time chemical composition

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Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng Zheng Wang Zhou Ying Mo Biao Zheng Miaomiao Han Qin zhong Wenwen Yang Peng Gao Lezhi Yang Jilei Liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

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Estimates of three classical summations on the spaces F_p^(α,q)(R^n)(0 < p ≤ 1)

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Applied Mathematics(A Journal of Chinese Universities) 2014年第3期29卷 329-338页

作者： GAO Gui-lian zhong Yong School of Science Hangzhou Dianzi University Postdoctoral Programme of Economics and Management School of Wuhan University Postdoctoral Programme of China Great Wall Asset Management Corporation

We obtain the boundedness on Fp^α,q（R^n）for the Poisson summation and Gauss summation. Their maximal operators are proved to be bounded from Fp^α,q（R^n）to L∞（R^n）For the maximal operator of the Bochner-Riesz ... 详细信息

关键词： Poisson summation Gauss summation Bochner-Riesz summation Triebel-Lizorkin space.

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Recent Advances in Nickel Catalyzed Carbonylative Reactions via the Insertion of Carbon Monoxide

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Chinese Journal of Chemistry 2024年第2期42卷 177-189页

作者： Xinzhou Chen Gang Chen zhong lian Department of Dermatology State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengduSichuan 610041China

Carbonyl compounds have attracted considerable attention due to their extensive applications in drug ***,they are important synthetic intermediates for the construction of carbon-carbon and carbon-heteroatom ***-metal-catalyzed carbonylation via the insertion of co is one of the most efficient and straightforward strategies to access carbonyl ***,most of the transition-metal-catalyzed carbonylative reactions require expensive and toxic noble-metal ***,there is a growing demand for the exploration of nickel-catalyzed carbonylative reactions via the insertion of CO due to the earth abundance and low cost of *** with the well-established palladium-catalyzed carbonylative reactions,nickel-catalyzed analogous transformations have been relatively *** is primarily because CO strongly binds to nickel,often resulting in catalyst *** recent years,some research groups have focused on using CO surrogates or NN_(2) pincer nickel catalyst to circumvent the formation of Ni(CO)_(4).Nickel-catalyzed carbonylation has been applied in the construction of carbonyl-containing compounds,such as ketones,carboxylic acids,thioesters,acyl chloride and carboxamides.

关键词： Carbonylation Nickel catalysis Carbon monoxide C1 chemistry Carbonyl-containing compounds

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Temporal Second-order Scheme for a Hidden-memory Variable Order Time Fractional Diffusion Equation with an Initial Singularity

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Acta Mathematicae Applicatae Sinica 2024年第4期40卷 1060-1077页

作者： Rui-lian DU Zhi-zhong SUN School of Big Data Changzhou UniversityChangzhou 213164China Department of Mathematics Shanghai UniversityShanghai 200444China School of Mathematics Southeast UniversityNanjing 210096China

In this work,a novel time-stepping L1 formula is developed for a hidden-memory variable-order Caputo’s fractional derivative with an initial *** formula can obtain second-order accuracy and an error estimate is analyzed *** an application,a fully discrete difference scheme is established for the initial-boundary value problem of a hidden-memory variable-order time fractional diffusion *** experiments are provided to support our theoretical results.

关键词： time fractional diffusion equation hidden-memory variable-order fractional derivative error estimate initial singularity

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An end-to-end 3D seismic simulation of underground structures due to point dislocation source by using an FK-FEM hybrid approach

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Frontiers of Structural and Civil Engineering 2022年第12期16卷 1515-1529页

作者： Zhenning ba Jisai FU Zhihui ZHU Hao zhong State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin UniversityTianjin 300072China School of Civil Engineering Tianjin UniversityTianjin 300350China National Engineering Research Center of High Speed Railway Construction Technology Central South UniversityChangsha 410075China School of Civil Engineering Central South UniversityChangsha 410075China

based on the domain reduction idea and artificial boundary substructure method,this paper proposes an FK-FEM hybrid approach by integrating the advantages of FK and FEM(i.e.,FK can efficiently generate high-frequency three translational motion,while FEM has rich elements types and constitutive models).An advantage of this approach is that it realizes the entire process simulation from point dislocation source to underground *** with the plane wave field input method,the FK-FEM hybrid approach can reflect the spatial variability of seismic motion and the influence of source and propagation *** approach can provide an effective solution for seismic analysis of underground structures under scenario of earthquake in regions where strong earthquakes may occur but are not recorded,especially when active faults,crustal,and soil parameters are *** Daikai subway station as an example,the seismic response of the underground structure is simulated after verifying the correctness of the approach and the effects of crustal velocity structure and source parameters on the seismic response of Daikai station are *** this example,the influence of velocity structure on the maximum interlayer displacement angle of underground structure is 96.5%and the change of source parameters can lead to the change of structural failure direction.

关键词： source-to-structure simulation FK-FEM hybrid approach underground structures point dislocation source

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑Yuan Yu Jing Wu Guang Yin Fan‑Zhen Jiao zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation bacterial cellulose Cobalt hydroxycarbonate nanorods

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