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Virologica Sinica 2021年第2期36卷 176-186页

作者： Yisha Zhuo Zeheng Guo Tongtong ba Cheng Zhang Lihua He Cuiping Zeng Hanchuan Dai College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China

African swine fever(ASF)is an infectious transboundary disease of domestic pigs and wild boar and spreading throughout *** is no vaccine and treatment *** immune escape strategies of African swine fever virus(ASFV)are crucial factors affecting immune prevention and vaccine ***360 genes have been implicated in the modulation of the IFN-Ⅰ*** molecular mechanisms contributing to innate immunity are poorly *** this study,we demonstrated that ASFV MGF360-12 L(MGF360 families 12 L protein)significantly inhibited the mRNA transcription and promoter activity of IFN-βand NF-κB,accompanied by decreases of IRF3,STING,TBK1,ISG54,ISG56 and AP-1 m RNA ***,MGF360-12 L might suppress the nuclear localization of p50 and p65 mediated by classical nuclear localization signal(NLS).Additionally,MGF360-12 L could interact with KPNA2,KPNA3,and KPNA4,which interrupted the interaction between p65 and KPNA2,KPNA3,*** further found that MGF360-12 L could interfere with the NF-κB nuclear translocation by competitively inhibiting the interaction between NF-κB and nuclear transport *** findings suggested that MGF360-12 L could inhibit the IFN-Ⅰproduction by blocking the interaction of importinαand NF-κB signaling pathway,which might reveal a novel strategy for ASFV to escape the host innate immune response.

关键词： African swine fever virus(ASFV) MGF360-12L IFN-Ⅰ NF-κB Nuclear transport

Polymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred(Landrace,Duroc,and Yorkshire)pigs:implications for the vaccine development of African swine fever virus

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Science China(Life Sciences) 2020年第10期63卷 1604-1607页

作者： Limin ba Zhenbao Wang William j Liu Dongxun Wu Wangzhen Xiang Peng Qi Chunna Dong Yanxin Hu Ping Lu jin Xiao Changyuan Yu College of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China Zhongmu Research Institute China Animal Husbandry Industry Co.LTDBeijing 100095China China Animal Husbandry Industry Co. LTDBeijing 100095China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052China Key Laboratory of Zoonosis of Ministry of Agriculture College of Veterinary MedicineChina Agricultural UniversityBeijing 100094China China Animal Health and Epidemiology Center Qingdao 266032China

Dear Editor,Swine major histocompatibility complex(MHC)is a highly polymorphic gene in pigs and is also called swine leukocyte antigen(SLA)(Fan et al.,2018).SLA is divided into three major categories,SLA I(SLA-1,-2,-3),SLA II,and SLA III(Smith et al.,2005).SLA I plays an important role in cellular immunity which can eliminate viruses and other foreign antigens in pigs(Macdonald et al.,2010).SLA-1,SLA-2 and SLA-3 are important SLA I alleles and are highly *** causes extreme difficulties in screening broadly protective cytotoxic lymphocyte(CTL)epitope *** supertype which is built based on peptide-binding specificity of each SLA I can overcome this difficulty to some extent(Thomsen et al.,2013).

关键词： swine Swine protective

Potential Anti-cancer Activity of Furanodiene

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Chinese journal of Cancer Research 2009年第2期21卷 154-158页

作者： Zhen-zhen ba Yan-ping Zheng Hui Zhang Xiu-yan Sun Dong-hai Lin School of Pharmaceutical Science Yantai University Yantai 264005 China

Objective： To study the isolated from the essential oil VIVO anti-tumor activities of furanodiene of the rhizome of Curcuma wenyujin （C15H200）, a primary sesquiterpene compound YH Chen et C. Ling（Wen Ezhu）, in vitro and in Methods： In vitro MTT assay was used to further study the effects of time and dosage on anti-proliferation of furanodiene against the sensitive Hela, Hep-2, HL-60, U251 cells, based on the cytotoxic effects of furanodiene on 12 human malignant tumor cell lines with the essential oil of Wen Ezhn as control., and the half-inhibitory concentration （IC50） was observed. In vivo uterine cervix （U14） tumor cell was selected and the conventional assay method of anti-tumor activity was employed. Furanodiene liposome was administered intraperitoneally, and tumor-inhibitory rate, thymus and spleen indexes were observed. Results： The inhibitive effects on cell proliferation were shown in all of the twelve cell lines and the cytotoxic effects of furanodiene against Hela, Hep-2, HL-60, U251 cells were observed after 12 h of administration, the effect could last for at least 48 h in a dose dependent manner, and the IC50 values were 0.6, 1.7, 1.8, 7.0μg/ml, respectively. Furanodiene was also found to show inhibitive effects on the proliferation of uterine cervix （U14） tumor induced in mice. The tumor inhibition rates were 36.09% （40 mg/kg）, 41.55% （60 mg/kg）, 58.29% （80 mg/kg）, respectively. Conclusion： Furanodiene is one of primary anti-cancer active components in the essential oil of Wen Ezhu, and also a very effective agent against uterine cervix cancer, and has protection effect on the immune function.

关键词： Essential oil of Wen Ezhu Furanodiene Anti-cancer Uterine cervix cancer

Reduction of dietary crude protein and feed form:Impact on broiler litter quality,ammonia concentrations,excreta composition,performance,welfare,and meat quality

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Animal Nutrition 2022年第2期9卷 291-303页

作者： Madri Brink Geert P.j.janssens Peter Demeyer Ozer bagci Evelyne Delezie Flanders Research Institute for Agriculture Fisheries and Food(ILVO)Animal Sciences UnitScheldeweg 689090 MelleBelgium Department of Nutrition Genetics and EthologyGhent UniversityHeidestraat 199820 MerelbekeBelgium Flanders Research Institute for Agriculture Fisheries and Food(ILVO)Agricultural Engineering UnitBurg.Van Gansberghelaan 1159820 MerelbekeBelgium Department of Mechanical Engineering Eindhoven University of Technology5600 MB-EindhovenNetherlands.

Nitrogen(N)excreted by poultry is converted to ammonia(NH3),presenting an environmental risk and a health risk to the farmer and animals.A study was performed to investigate the effect of reduced CP and feed form on broiler performance and welfare,meat and litter quality,N utilization,and NH3 concentrations at litter level.A total of 2,232 Ross 308 male broilers was divided into 6 treatments and 6 replicates,which was fed diets in both pellet and mash forms with different CP levels of 205.0 g/kg(H,high),187.5 g/kg(M,intermediate)and 175.0 g/kg(L,low)in the grower phase and 195.0 g/kg(H),180.0 g/kg(M)and 165.6 g/kg(L)in the finisher *** amino acids(AA)were supplemented to maintain digestible AA-to-digestible lysine *** dietary CP content to 187.5 g/kg in the grower phase and 180.0 g/kg in the finisher phase reduced NH3 concentrations at litter level(P<0.001),but a further reduction in dietary CP had no additional *** treatments had better litter qualities and lower incidences of foot and hock lesions than pellet treatments at d 38(P<0.001).In addition,treatments with reduced CP had lower incidence of foot lesions at d 38(P<0.001).Broilers fed pelleted diets had higher ADFI,ADG,and final BW,improved feed conversion ratio(FCR),and heavier carcasses(P<0.001)than those fed mash diets over a production period of 39 *** could not be maintained when birds were fed L CP pelleted *** study demonstrated that,with the supplementation of AA to meet requirements,the concentration of dietary CP can be reduced to 187.5 and 180.0 g/kg in the grower and finisher phases respectively,without impairing broiler performance,meat yield and *** diets were favorable when considering the overall litter quality and welfare of the ***,they could not maintain the same broiler performance and slaughter yield as pelleted *** from the present study may assist the poultry sector towards a socially acceptable lowemission

关键词： Broiler Ammonia Crude protein Feed form Performance

疼痛管理模式下不同绷带包扎时间对全膝关节置换术后患者早期功能恢复的影响

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中国医药指南 2024年第1期22卷 7-9页

作者：巴婕姚晓红曾惠玲刘琼王艳琪中山市中医院骨三科中山528400

目的研究疼痛管理模式下不同绷带包扎时间对全膝关节置换术后患者早期功能恢复的影响。方法选择2022年6月至2023年5月单侧全膝关节置换手术(TKA)患者80例,根据弹力绷带使用时间,24 h为对照组(40例),48 h为研究组(40例);观察两组围手术... 详细信息

目的研究疼痛管理模式下不同绷带包扎时间对全膝关节置换术后患者早期功能恢复的影响。方法选择2022年6月至2023年5月单侧全膝关节置换手术(TKA)患者80例,根据弹力绷带使用时间,24 h为对照组(40例),48 h为研究组(40例);观察两组围手术期术侧下肢的周径、膝关节功能、伤口并发症、相关不良反应,随访期间步速、步频、步幅。结果术后7 d、14 d大腿、膝关节、小腿周径均短于对照组;术后7 d、30 d膝关节活动度(ROM)屈、伸均大于对照组,膝关节功能评分(HSS)高于对照组;术后3个月两组均出现好转,研究组步速、步频、步幅均高于对照组(P0.05)。结论全膝关节置换术后患者在疼痛管理模式下,使用48 d弹力绷带,能够减轻术肢肿胀,增加肢体的屈伸活动,有利于膝关节功能恢复。

关键词：疼痛不同绷带包扎时间全膝关节置换功能恢复

Diamonds and Other Exotic Minerals Recovered from Peridotites of the Dangqiong Ophiolite, Western Yarlung-Zangbo Suture Zone, Tibet

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Acta Geologica Sinica(English Edition) 2016年第2期90卷 425-439页

作者： XIONG Fahui YANG jingsui ROBINSON Paul T. XU Xiangzhen ba Dengzhu LI Yuan ZHANG Zhongming RONG He CARMA State Key Laboratory for Continental Tectonics and Dynamics Institute of Geology Chinese Academy of Geological Sciences

Various combinations of diamond, moissanite, zircon, quartz, corundum, rutile, titanite, almandine garnet, kyanite, and andalusite have been recovered from the Dangqiong peridotites. More than 80 grains of diamond have been recovered, most of which are pale yellow to reddish-orange to colorless. The grains are all 100-200 μm in size and mostly anhedral, but with a range of morphologies including elongated, octahedral and subhedral varieties. Their identification was confirmed by a characteristic shift in the Raman spectra between 1325 cm^-1 and 1333 cm^-1, mostly at 1331.51 cm^-1 or 1326.96 cm^-1. Integration of the mineralogical, petrological and geochemical data for the Dongqiong peridotites suggests a multi-stage formation for this body and similar ophiolites in the Yarlung-Zangbo suture zone. Chromian spinel grains and perhaps small bodies of chromitite crystallized at various depths in the upper mantle, and encapsulated the UHP, highly reduced and crustal minerals. Some oceanic crustal slabs containing the chromian spinel and their inclusion were later trapped in suprasubduction zones（SSZ）, where they were modified by island arc tholeiitic and boninitic magmas, thus changing the chromian spinel compositions and depositing chromitite ores in melt channels.

关键词： diamond Multi-stage formation Dangqiong ophiolite Yarlung-Zangbo suture zone

US Georgetown University President john DeGioia Leads Delegation to China

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Voice of Friendship 2009年第4期 17-18页

作者： ba Cuicui

Invited by the CPAFFC, john j. DeGioia, president of Georgetown University of the U. S.,and his party visited Gansu, jiangxi and Hubei provinces and Beijing from March 4 to 9. Liu Yandong, state councillor; Li Yuancha... 详细信息

关键词： visited jiangxi party Hubei president delegation c

Alkaline activation of endogenous latent TGFβ1 by an injectable hydrogel directs cell homing for in situ complex tissue regeneration

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Bioactive Materials 2022年第9期7卷 316-329页

作者： Sainan Wang Yuting Niu Peipei jia Zheting Liao Weimin Guo Rodrigo Cotrim Chaves Khanh-Hoa Tran-ba Ling He Hanying bai Sam Sia Laura j.Kaufman Xiaoyan Wang Yongsheng Zhou Yanmei Dong jeremy j.Mao Department of Cariology and Endodontology Peking University School and Hospital of StomatologyBeijing100081China Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China Center for Craniofacial Regeneration Columbia UniversityNew YorkNY10032USA Department of Biomedical Engineering Columbia UniversityNew YorkNY10027USA Department of Chemistry Columbia UniversityNew YorkNY10027USA

Utilization of the body’s regenerative potential for tissue repair is known as in situ tissue ***,the use of exogenous growth factors requires delicate control of the dose and delivery strategies and may be accompanied by safety,efficacy and cost *** this study,we developed,for the first time,a biomaterial-based strategy to activate endogenous transforming growth factor beta 1(TGFβ1)under alkaline conditions for effective in situ tissue *** demonstrated that alkaline-activated TGFβ1 from blood serum,bone marrow fluids and soaking solutions of meniscus and tooth dentin was capable of increasing cell recruitment and early differentiation,implying its broad ***,we engineered an injectable hydrogel(MS-Gel)consisting of gelatin microspheres for loading strong alkaline substances and a modified gelatin matrix for hydrogel click *** vitro models showed that alkaline MS-Gel controllably and sustainably activated endogenous TGFβ1 from tooth dentin for robust bone marrow stem cell *** importantly,infusion of in vivo porcine prepared root canals with alkaline MS-Gel promoted significant pulp-dentin regeneration with neurovascular stroma and mineralized tissue by endogenous proliferative ***,this work offers a new bench-to-beside translation strategy using biomaterial-activated endogenous biomolecules to achieve in situ tissue regeneration without the need for cell or protein delivery.

关键词： Endogenous TGFβ1 Injectable alkaline hydrogel Cell homing Pulp-dentin complex In situ tissue regeneration

SR8278改善时差所致小鼠泪腺功能紊乱的转录组学分析

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眼科新进展 2024年第4期44卷 264-269页

作者：黄深振齐迪裴晓婷路顶立司宏丽黄杜鎏睿张文潇巴梦茹轩书婷李志杰郑州大学人民医院河南省人民医院河南大学人民医院河南省立眼科医院河南省眼科研究所河南省郑州市450003

目的探讨核受体亚家族1 D组成员1(NR1D1)合成拮抗剂SR8278在改善时差诱导的小鼠眶外泪腺结构和功能损伤方面的作用及其机制。方法 36只健康的8~10周龄野生型C57BL/6j小鼠在12 h光照/12 h黑暗循环的昼夜节律箱内适应2周后随机分为3组:... 详细信息

目的探讨核受体亚家族1 D组成员1(NR1D1)合成拮抗剂SR8278在改善时差诱导的小鼠眶外泪腺结构和功能损伤方面的作用及其机制。方法 36只健康的8~10周龄野生型C57BL/6j小鼠在12 h光照/12 h黑暗循环的昼夜节律箱内适应2周后随机分为3组:正常组(12 h光照/12 h黑暗循环的昼夜节律箱饲养)、时差组(8 h前移的12 h光照/12 h黑暗循环的昼夜节律箱饲养)及时差+SR8278组(8 h前移的12 h光照/12 h黑暗循环的昼夜节律箱饲养,并给予25 mg·kg^(-1)的SR8278干预),每组12只,共干预5 d。干预结束后,收集各组小鼠的活动度和核心体温,记录小鼠的泪液分泌量,并对泪腺组织进行称重和泪腺细胞大小测量。采用免疫组织化学方法对小鼠眶外泪腺进行组织学评估。提取泪腺RNA进行包含NR1D1的高通量RNA-Seq分析,并对获得的转录组数据进行KEGG和GO功能富集分析。结果与正常组相比,时差组小鼠白天活动度较高,夜间活动度较低,白天核心体温较高,夜间核心体温较低,差异均有统计学意义(均为P<0.05)。与时差组相比,时差+SR8278组小鼠白天活动度较低,夜间活动度较高,白天核心体温较低,夜间核心体温较高,差异均有统计学意义(均为P<0.05)。与正常组相比,时差组小鼠泪腺重量及泪液分泌量降低,泪腺细胞增大,差异均有统计学意义(均为P<0.05);与时差组相比,时差+SR8278组小鼠泪腺重量及泪液分泌量增加,泪腺细胞减小,差异均有统计学意义(均为P<0.05)。与正常组相比,时差组小鼠夜晚泪腺中NR1D1表达量较高;与时差组相比,时差+SR8278组小鼠夜晚泪腺中NR1D1表达量较低,差异均有统计学意义(均为P<0.05)。生物信息学分析显示,时差组和时差+SR8278组共有947个显著性差异基因,其中显著性上调基因43个,显著性下调基因904个,信号通路差异最大的为Notch信号通路。结论 SR8278通过靶向抑制NR1D1,有效增强了时差小鼠的泪液分泌功能,其过程可能是通过Notch信号通路完成的。

关键词：时差 SR8278 泪腺功能紊乱小鼠

INFLUENCE OF PULSED MAGNETIC FIELD ON MICROSTRUC-TURES AND MACRO-SEGREGATION IN 2124 Al-ALLOY

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Acta Metallurgica Sinica(English Letters) 2002年第4期15卷 380-384页

作者： C.Y.ban,j.Z.Cui,Q.X.ba,G.M.Lu and B.j.ZhangP.O. Box 317, The Key Laboratory of Electromagnetic Processing of Materials, Ministry of Education, Northeastern University, Shenyang 110004, China Manuscript received 10 October 2001 Northeastern Univ. Lab. of Electr. Proc. of Mat. Shenyang 110004 China

The structures and macro-segregation of 2124 Al-alloy were studied when a pulsed magnetic field (PMF) was applied during solidification. It is found through experi-ments that a remarkable change occurs in the solidification structures of 2124 Al-alloy under pulsed magnetic field. The eutectic phase at grain boundaries change from thick continuous eutectic network to thin discontinuous one, and the distribution of solute elements was also homogenized. The typical negative segregation phenomenon of Cu in common solidification condition was restrained, and the segregation of Mg decreased.

关键词： aluminium alloy pulsed magnetic field microstructure segrega-tion