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Enhanced potency of an IgM-like nanobody targeting conserved epitope in SARS-CoV-2 spike N-terminal domain

Signal Transduction and Targeted Therapy 2024年第6期9卷 2726-2737页

作者： bo liu Honghui liu Pu Han Xiaoyun Wang Chunmei Wang Xinxin Yan Wenwen Lei Ke Xu Jianjie Zhou Jianxun Qi Ruiwen Fan Guizhen Wu Wen-xia Tian George F.Gao Qihui Wang College of Veterinary Medicine Shanxi Agricultural University030801 JinzhongChina CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)100101 BeijingChina School of Life Sciences Yunnan University650504 KunmingYunnan ProvinceChina NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)102206 BeijingChina Savaid Medical School University of Chinese Academy of Sciences101408 BeijingChina

Almost all the neutralizing antibodies targeting the receptor-binding domain(RBD)of spike(S)protein show weakened or lost efficacy against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)emerged or emerging variants,such as Omicron and its *** suggests that highly conserved epitopes are crucial for the development of neutralizing ***,we present one nanobody,N235,displaying broad neutralization against the SARS-CoV-2 prototype and multiple variants,including the newly emerged Omicron and its ***-electron microscopy demonstrates N235 binds a novel,conserved,cryptic epitope in the N-terminal domain(NTD)of the S protein,which interferes with the RBD in the neighboring S *** neutralization mechanism interpreted via flow cytometry and Western blot shows that N235 appears to induce the S1 subunit shedding from the trimeric S ***,a nano-IgM construct(MN235),engineered by fusing N235 with the human IgM Fc region,displays prevention via inducing S1 shedding and cross-linking virus *** to N235,MN235 exhibits varied enhancement in neutralization against pseudotyped and authentic viruses in *** intranasal administration of MN235 in low doses can effectively prevent the infection of Omicron sub-variant BA.1 and XBB in vivo,suggesting that it can be developed as a promising prophylactic antibody to cope with the ongoing and future infection.

关键词： doses prevention conserved

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Hydromassage of macular hole edges for large and persistent full-thickness macular holes

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 551-557页

作者： Yi Cai Wen-bo liu Duo Wei Xun Deng Xiao-Xin Li Ming-Wei Zhao Xuan Shi Jian-Hong Liang Department of Ophthalmology Peking University People’s HospitalBeijing 100044China Eye Diseases and Optometry Institute Beijing 100044China Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases Beijing 100044China College of Optometry Peking University Health Science CenterBeijing 100044China Xiamen Eye Center of Xiamen University Xiamen 361102Fujian ProvinceChina Department of Ophthalmology the Second Hospital of Lanzhou UniversityLanzhou 730030Gansu ProvinceChina

●AIM:To introduce the macular hole(MH)hydromassage technique as a potentially beneficial approach for the treatment of large or persistent MH.●METHODS:This retrospective observational case series comprised 16 consecutive patients(17 eyes)diagnosed with *** criteria involved a hole aperture diameter larger than 600μm or the presence of an unclosed MH larger than 600μm following the previous *** MH repair procedures were administered in all cases,involving the manipulation and aspiration of the hole margin through the application of water flow with a soft-tip flute needle.A comprehensive assessment was conducted for each case before and after surgery,and optical coherence tomography(OCT)images were captured at every follow-up point.●RESULTS:The mean preoperative aperture diameter was 747±156μm(range 611-1180μm),with a mean base diameter of 1390±435μm(range 578-2220μm).Following surgery,all cases achieved complete anatomical closure of MH,with 13 cases(76.5%)exhibiting type 1 closure and 4 cases(23.5%)demonstrating type 2 *** significant differences were observed in the preoperative OCT variables between the two closure *** with type 1 closure showed a significantly improved visual acuity(0.70±0.10,range 0.50-0.80)compared to those with type 2 closure(0.90±0.12,range 0.80-1.00,P=0.014).●CONCLUSION:The MH hydromassage technique demonstrates promising results,achieving acceptable closure rates in cases of large or persistent *** technique may serve as an effective adjunctive maneuver during challenging MH surgery.

关键词： macular hole large macular hole persistent macular hole optical coherence tomography surgical technique hydromassage

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Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses

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Virologica Sinica 2024年第1期39卷 169-172页

作者： Yabin Hu Jun Chen Jing Yang Zhanpeng liu Xiaoyue Zhang Qian Wu Lulu liu Shishan Teng Rongzhang He bo liu Xingyu Zheng Rui Lu Dong Pan You Wang Liting Peng Hongying chen Yi-Ping Li Wenpei liu Xiaowang Qu College of Basic Medical Sciences Hengyang Medical SchoolUniversity of South China&MOE Key Lab of Rare Pediatric DiseasesHengyang421001China Translational Medicine Institute The First People's Hospital of ChenzhouHengyang Medical SchoolUniversity of South ChinaChenzhou423000China College of Life Sciences Northwest A&F UniversityYangling712100China Institute of Human Virology Department of Pathogen Biology and Biosecurityand Key Laboratory of Tropical Disease Control of Ministry of EducationZhongshan School of MedicineSun Yat-sen UniversityGuangzhou510080China School of Public Health University of South ChinaHengyang421001China

Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),theseventh coronavirus known to jump from intermediate hosts to humans,has resulted in a worldwide pandemic and caused immense economicdamage(Tan et al.,2020;Zhu et al.,2020).It is believed that coronavirus spillover may occur again in the *** exposure to a bat coro-navirus has been serologically confimed in a rural area of China,indi-cating that the coronavirus spillover occurred,although the origin andfrequency of spillovers are unclear(Wang et al.,2018).

关键词： monoclonal acute

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Individualized anti-thrombotic therapy for acute myocardial infarction complicated with left ventricular thrombus: A case report

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World Journal of Clinical Cases 2024年第4期12卷 835-841页

作者： Yan Song Hua Li Xia Zhang Lei Wang Hong-Yan Xu Zhi-Chao Lu Xiao-Gang Wang bo liu Department of Clinical Pharmacy Zibo Municipal HospitalZibo 255400Shandong ProvinceChina Department of Cardiology Zibo Municipal HospitalZibo 255400Shandong ProvinceChina Department of Pharmacy Zibo Municipal HospitalZibo 255400Shandong ProvinceChina Department of Imaging Zibo Municipal HospitalZibo 255400Shandong ProvinceChina Department of Pulmonary and Critical Care Medicine Department of Clinical MicrobiologyZibo City Key Laboratory of Respiratory Infection and Clinical MicrobiologyZibo City Engineering Technology Research Center of Etiology Molecular DiagnosisZibo Municipal HospitalZibo 255400Shandong ProvinceChina

BACKGROUND Presently,there is no established standard anti-blood clot therapy for patients facing acute myocardial infarction(AMI)complicated by left ventricular thrombus(LVT).While vitamin K antagonists are the preferred choice for oral blood thinning,determining the best course of blood-thinning medication remains *** is unclear if non-vitamin K antagonist oral blood thinners have different effectiveness in treating *** study significantly contributes to the medical *** SUMMARY The blood-thinning treatment of a patient with AMI and LVT was *** blood-thinning therapy included daily enteric-coated aspirin tablets at 0.1 g,daily clopidogrel hydrogen sulfate at 75 mg,and dabigatran etexilate at 110 mg twice *** 15 d,the patient’s LVT did not decrease but instead *** pharmacists comprehensively analyzed the cases from the perspective of the patient’s disease status and drug *** drug regimen was reformulated for the patient,replacing dabigatran etexilate with warfarin,and was administered for six *** clinical pharmacist provided the patient with professional and standardized pharmaceutical *** patient’s condition was discharged after meeting the international normalized ratio value(2-3)*** patient fully complied with the follow-up,and the time in the therapeutic range was 78.57%,with no serious adverse effects during pharmaceutical *** Warfarin proves to be an effective drug for patients with AMI complicated by LVT,and its blood-thinning course lasts for six months.

关键词： Myocardial infarction Left ventricular thrombus Dabigatran etexilate Warfarin Clinical pharmacist Case report

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基于改进粒子滤波的锂离子电池剩余寿命预测

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电力系统保护与控制 2024年第9期52卷 123-131页

作者：刘博尹杰李然哈尔滨理工大学传感器与可靠性工程研究所黑龙江哈尔滨150080 汽车电子驱动控制与系统集成教育部工程研究中心黑龙江哈尔滨150080

针对锂离子电池剩余寿命预测精度低、泛化能力差等问题,提出基于改进粒子滤波的预测方案。首先,提出双高斯模型作为退化经验模型,拟合锂离子电池的容量退化过程。然后,通过先验知识设置退化模型的初始参数,并利用粒子滤波方法进行参数... 详细信息

针对锂离子电池剩余寿命预测精度低、泛化能力差等问题,提出基于改进粒子滤波的预测方案。首先,提出双高斯模型作为退化经验模型,拟合锂离子电池的容量退化过程。然后,通过先验知识设置退化模型的初始参数,并利用粒子滤波方法进行参数更新。针对预测过程中出现的粒子退化问题,提出高斯混合方法进行粒子重采样,拟合重采样过程中粒子复杂的非线性分布和长尾分布,保证预测结果的概率密度分布状况均匀且集中。最后在不同的数据集上进行了实验验证,结果表明所提出的改进粒子滤波方案具有较高的精度和较强的鲁棒性。

关键词：锂离子电池剩余寿命预测粒子滤波高斯混合模型

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全覆盖处方点评对门、急诊抗菌药物合理应用的影响

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中国抗生素杂志 2024年第4期49卷 476-480页

作者：刘波成都市第二人民医院药学部成都610011

目的观察对门、急诊所有抗菌药物处方进行全覆盖大样本量的点评对医院抗菌药物合理用药影响。方法对门、急诊所有抗菌药物处方进行点评,本文通过实践前后对照的方法,将点评模式优化前(2022年)、优化后(2023年)的点评结果即不合格率进行... 详细信息

目的观察对门、急诊所有抗菌药物处方进行全覆盖大样本量的点评对医院抗菌药物合理用药影响。方法对门、急诊所有抗菌药物处方进行点评,本文通过实践前后对照的方法,将点评模式优化前(2022年)、优化后(2023年)的点评结果即不合格率进行比较和统计学处理,计数资料采用χ2检验。结果对比优化前,门急诊抗菌药物处方不合格率进一步降低。2023年与2022年相比明显降低,差异有统计学意义(P<0.05)。结论通过全覆盖处方点评、人工点评结合软件点评、学术交流和行政干预并行等措施可显著降低抗菌药物处方不合格率,持续改进不合理用药现象。

关键词：处方点评抗菌药物不合理使用全覆盖

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环境保护税法与企业绿色创新——基于环境合法性和资源获取的链式中介模型

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研究与发展管理 2024年第4期36卷 113-127页

作者：刘博刘振南昌大学公共政策与管理学院南昌330031 郑州航空工业管理学院商学院郑州450046

环境保护税法是促进企业绿色创新的重要驱动力。基于环境合法性和资源依赖理论,采用2007—2022年中国A股上市公司的非平衡面板数据,以《环境保护税法》实施为准自然实验,使用倾向得分匹配和双重差分方法(PSM-DID),分析了中国环境保护税... 详细信息

环境保护税法是促进企业绿色创新的重要驱动力。基于环境合法性和资源依赖理论,采用2007—2022年中国A股上市公司的非平衡面板数据,以《环境保护税法》实施为准自然实验,使用倾向得分匹配和双重差分方法(PSM-DID),分析了中国环境保护税法对企业绿色创新的影响,环境合法性、绿色补贴和融资约束的链式中介效应以及市场垄断的调节效应。研究发现,环境保护税法的压力机制和激励机制对企业绿色创新具有促进作用;环境保护税法能够促进企业获取环境合法性,拥有环境合法性的企业能够获得绿色补贴和缓解融资约束,进而间接地促进企业绿色创新;市场垄断阻碍了环境保护税法对企业绿色创新的积极推动作用。研究结论不仅为理解环境保护税法如何影响企业绿色创新提供了新的视角,也为法制部门完善环境保护税法、政府部门遏制市场垄断以及企业环境合法性的获取等提供重要启示。

关键词：环境保护税法绿色创新环境合法性市场垄断

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Load Testing in Bridge Maintenance

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Journal of Architectural Research and Development 2024年第3期8卷 63-68页

作者： bo liu Xu Meng Ji Li Zhi Tu China Merchants Chongqing Highway Engineering Testing Center CO. LTD.Chongqing 400060China Chongqing Construction Science Research Inspection and Testing Co. Ltd.Chongqing 400060China

Highway bridges are a crucial component in road transportation *** bridges must be maintained according to usage requirements *** results must be considered before devising a maintenance *** testing is a vital method of assessing the quality and performance of highway *** outcomes of these tests facilitate the formulation of maintenance *** article examines the definition of load testing,its significance,and the process of execution,with the goal of providing support for bridge inspection and maintenance.

关键词： Highway bridge Load test and inspection Bridge maintenance

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Development of a new Cox model for predicting long-term survival in hepatitis cirrhosis patients underwent transjugular intrahepatic portosystemic shunts

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World Journal of Gastrointestinal Surgery 2024年第2期16卷 491-502页

作者： Yi-Fan Lv Bing Zhu Ming-Ming Meng Yi-Fan Wu Cheng-Bin Dong Yu Zhang bo-Wen liu Shao-Li You Sa Lv Yong-Ping Yang Fu-Quan liu Liver Disease Minimally Invasive Diagnosis and Treatment Center Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Liver Vascular Disease Diagnosis and Treatment Center The Fifth Medical Center of Chinese People's Liberation Army General HospitalBeijing 100039China

BACKGROUND Transjugular intrahepatic portosystemic shunt(TIPS)placement is a procedure that can effectively treat complications of portal hypertension,such as variceal bleeding and refractory ***,there have been no specific studies on predicting long-term survival after TIPS *** To establish a model to predict long-term survival in patients with hepatitis cirrhosis after *** A retrospective analysis was conducted on a cohort of 224 patients who un-derwent TIPS *** univariate and multivariate Cox regression analyses,various factors were examined for their ability to predict survival at 6 years after ***,a composite score was formulated,encompassing the indication,shunt reasonability,portal venous pressure gradient(PPG)after TIPS,percentage decrease in portal venous pressure(PVP),indocyanine green retention rate at 15 min(ICGR15)and total bilirubin(Tbil)***,the performance of the newly developed Cox(NDC)model was evaluated in an in-ternal validation cohort and compared with that of a series of existing *** The indication(variceal bleeding or ascites),shunt reasonability(reasonable or unreasonable),ICGR15,post-operative PPG,percentage of PVP decrease and Tbil were found to be independent factors affecting long-term survival after TIPS *** NDC model incorporated these parameters and successfully identified patients at high risk,exhibiting a notably elevated mortality rate following the TIPS procedure,as observed in both the training and validation ***,in terms of predicting the long-term survival rate,the performance of the NDC model was significantly better than that of the other four models[Child-Pugh,model for end-stage liver disease(MELD),MELD-sodium and the Freiburg index of post-TIPS survival].CONCLUSION The NDC model can accurately predict long-term survival after the TIPS procedure in patients with hepatitis cirrhosis,help identify high-risk patients

关键词： Transjugular intrahepatic portosystemic shunt Long-term survival Predictive model

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A telomere-to-telomere genome assembly of Zhonghuang 13,a widely-grown soybean variety from the original center of Glycine max

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The Crop Journal 2024年第1期12卷 142-153页

作者： Anqi Zhang Tangchao Kong Baiquan Sun Shizheng Qiu Jiahe Guo Shuyong Ruan Yu Guo Jirui Guo Zhishuai Zhang Yue liu Zheng Hu Tao Jiang Yadong liu Shuqi Cao Shi Sun Tingting Wu Huilong Hong Bingjun Jiang Maoxiang Yang Xiangyu Yao Yang Hu bo liu Tianfu Han Yadong Wang Center for Bioinformatics School of Computer Science and TechnologyHarbin Institute of TechnologyHarbin 150001HeilongjiangChina Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Soybean(Glycine max)stands as a globally significant agricultural crop,and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary ***,previous soybean genome assemblies have harbored gaps and incompleteness,which have constrained in-depth investigations into ***,we present Telomere-to-Telomere(T2T)assembly of the Chinese soybean cultivar Zhonghuang 13(ZH13)genome,termed ZH13-T2T,utilizing PacBio Hifi and ONT ultralong *** employed a multi-assembler approach,integrating Hifiasm,NextDenovo,and Canu,to minimize biases and enhance assembly *** assembly spans 1,015,024,879 bp,effectively resolving all 393 gaps that previously plagued the reference *** annotation efforts identified 50,564 high-confidence protein-coding genes,707 of which are ***13-T2T revealed longer chromosomes,421 not-aligned regions(NARs),112 structure variations(SVs),and a substantial expansion of repetitive element compared to earlier ***,we identified 25.67 Mb of tandem repeats,an enrichment of 5S and 48S rDNAs,and characterized their genotypic *** summary,we deliver the first complete Chinese soybean cultivar T2T *** comprehensive annotation,along with precise centromere and telomere characterization,as well as insights into structural variations,further enhance our understanding of soybean genetics and evolution.

关键词： Soybean Telomere-to-Telomere assembly Zhonghuang 13 Structure variations

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