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High-density lipoprotein regulates angiogenesis by affecting autophagy via miRNA-181a-5p

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Science China(Life Sciences) 2024年第2期67卷 286-300页

作者： bi-Ang Kang Hua-ming Li Ya-Ting Chen Meng-Jie Deng Yan Li Yue-ming Peng Jian-Jun Gao zhi-Wei Mo Jia-Guo Zhou zhi-Jun Ou Jing-Song Ou Division of Cardiac Surgery Cardiovascular Diseases InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China National-Guangdong Joint Engineering Laboratory for Diagnosis and Treatment of Vascular Diseases Guangzhou 510080China NHC key Laboratory of Assisted Circulation(Sun Yat-sen University) Guangzhou 510080China Guangdong Provincial Engineering and Technology Center for Diagnosis and Treatment of Vascular Diseases Guangzhou 510080China Division of Vascular Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Department of Pharmacology Cardiac and Cerebral Vascular Research CenterZhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Division of Hypertension and Vascular Diseases Department of CardiologyCardiovascular Diseases InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Guangdong Provincial Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China

We previously demonstrated that normal high-density lipoprotein(nHDL)can promote angiogenesis,whereas HDL from patients with coronary artery disease(d HDL)is dysfunctional and impairs *** plays a critical role in angiogenesis,and HDL regulates ***,it is unclear whether n HDL and d HDL regulate angiogenesis by affecting *** cells(ECs)were treated with n HDL and d HDL with or without an autophagy ***,endothelial nitric oxide synthase(e NOS)expression,miRNA expression,nitric oxide(NO)production,superoxide anion(O2^(·-))generation,EC migration,and tube formation were evaluated.n HDL suppressed the expression of miR-181a-5p,which promotes autophagy and the expression of e NOS,resulting in NO production and the inhibition of O2^(·-)generation,and ultimately increasing in EC migration and tube formation.d HDL showed opposite effects compared to n HDL and ultimately inhibited EC migration and tube *** found that autophagy-related protein 5(ATG5)was a direct target of ***5 silencing or miR-181a-5p mimic inhibited n HDL-induced autophagy,e NOS expression,NO production,EC migration,tube formation,and enhanced O2^(·-)generation,whereas overexpression of ATG5 or miR-181a-5p inhibitor reversed the above effects of d ***5 expression and angiogenesis were decreased in the ischemic lower limbs of hypercholesterolemic low-density lipoprotein receptor null(LDLr^(-/-))mice when compared to C57BL/6 ***5 overexpression improved angiogenesis in ischemic hypercholesterolemic LDLr^(-/-)*** together,nHDL was able to stimulate autophagy by suppressing miR-181a-5p,subsequently increasing e NOS expression,which generated NO and promoted *** contrast,d HDL inhibited angiogenesis,at least partially,by increasing miR-181a-5p expression,which decreased autophagy and e NOS expression,resulting in a decrease in NO production and an increase in O2^(·-)*** findings reveal a nov

关键词： high-density lipoprotein angiogenesis autophagy ATG5 miRNA endothelial nitric oxide synthase

基于TORA的移动Ad hoc网络多径路由协议M-TORA

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北京理工大学学报 2006年第5期26卷 442-446页

作者：刘强匡镜明王华毕志明北京理工大学信息科学技术学院电子工程系北京100081

基于临时按序路由算法(TORA),提出一种移动Ad hoc网络(MANET)多径路由协议M-TORA.通过在互联网封装协议(IMEP)分组的头域中加入媒体接入控制(MAC)层信息,M-TORA在节点拥有多条下行链路的情况下能够综合考虑下行链路的路由跳数与MAC层缓... 详细信息

基于临时按序路由算法(TORA),提出一种移动Ad hoc网络(MANET)多径路由协议M-TORA.通过在互联网封装协议(IMEP)分组的头域中加入媒体接入控制(MAC)层信息,M-TORA在节点拥有多条下行链路的情况下能够综合考虑下行链路的路由跳数与MAC层缓存队列长度,从而选择出最佳的下行链路.M-TORA在进行多径路由选择时采用了基于概率的路由选择策略,将网络负荷分散到多条路由中并起到自动负荷平衡的作用.基于网络仿真软件OPNET对TORA和M-TORA的仿真表明:M-TORA路由协议能够有效地降低路由延时,提高网络分组投递率.

关键词：临时按序路由算法 M-TORA Ad hoc 移动自组织网络多径路由协议

Comprehensive analysis of chemical constituents in Xingxiong injection by high performance liquid chromatography coupled with mass spectrometry

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Chinese Journal of Natural Medicines 2015年第9期13卷 711-720页

作者： GUO Long DOU Li-Li DUAN Li LIU Ke bi zhi-ming LI Ping LIU E-Hu State Key Laboratory of Natural Medicines China Pharmaceutical University

Xingxiong injection(XXI) is a widely used Chinese herbal formula prepared by the folium ginkgo extract and ligustrazine for the treatment of cardiovascular and cerebrovascular diseases. Compared with the pharmacological studies, chemical analysis and quality control studies on this formula are relatively limited. In the present study, a high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-QTOF MS) method was applied to comprehensive analysis of constituents in XXI. According to the fragmentation rules and previous reports, thirty ginkgo flavonoids, four ginkgo terpene lactones, and one alkaloid were identified. A high performance liquid chromatography coupled with triple quadrupole mass spectrometry(HPLC-QQQ MS) method was then applied to quantify ten major constituents in XXI. The method validation results indicated that the developed method had desirable specificity, linearity, precision and accuracy. The total contents of ginkgo flavonoids were about 22.05-25.51 μg·mL-1 and the ginkgo terpene lactones amounts were about 4.41-8.70 μg·m L-1 in six batches of XXI samples, respectively. Furthermore, cosine ratio algorithm and distance measurements were employed to evaluate the similarity of XXI samples, and the results demonstrated a high-quality consistency. This work could provide comprehensive information on the quality control of Xingxiong injection, which be helpful in the establishment of a rational quality control standard.

关键词： Xingxiong injection Favonoids Terpene lactones HPLC-QTOF MS HPLC-QQQ MS

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黄芪皂苷注射液指纹图谱的研究

中国中药杂志 2006年第10期31卷 807-809页

作者：管佳毕志明李萍中国药科大学生药学教研室江苏南京210038

目的：建立黄芪皂苷类成分注射液（包括药材、中间体及注射液制剂）的HPIE-ELSD指纹图谱质量控制方法。方法：Agilent Zorbax SB-C18柱，流动相为A水-B乙腈，二元梯度洗脱：15％～60％B（0—65min），流速0．8mL·mi^-1，蒸发光散射检... 详细信息

目的：建立黄芪皂苷类成分注射液（包括药材、中间体及注射液制剂）的HPIE-ELSD指纹图谱质量控制方法。方法：Agilent Zorbax SB-C18柱，流动相为A水-B乙腈，二元梯度洗脱：15％～60％B（0—65min），流速0．8mL·mi^-1，蒸发光散射检测器，漂移管温度105℃，载气流速2．7L·min^-1。结果：药材、中间体、注射液制剂中标定的各共有峰相对保留时间的RSD值均小于1．0％，单峰面积大于或等于10％峰的峰面积比值也比较稳定，三者的图谱间具有很好的相关性，10批样品间具有很高的相似度。结论：本方法可作为黄芪皂苷注射液质量控制的依据。

关键词：指纹图谱黄芪皂苷注射液

A Chinese Multi-Specialty Delphi Consensus to Optimize RAASi Usage and Hyperkalaemia Management in Patients with Chronic Kidney Disease and Heart Failure

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Chinese Medical Sciences Journal 2024年第2期39卷 79-90页

作者： ming-Hui Zhao Wei Chen Hong Cheng bi-Cheng Liu zhi-Guo Mao Zhuang Tian Gang Xu Jing-Min Zhou Renal Division Peking University First HospitalBeijing 100034China Department of Nephrology The First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510080China Department of Nephrology Capital Medical UniversityBeijing 100029China Department of Nephrology Southeast University Zhongda HospitalNanjing 210009China Department of Nephrology Shanghai Changzheng HospitalShanghai 200003China Department of Cardiology Peking Union Medical College HospitalBeijing 100730China Renal Division Tongji Medical College of Huazhong University of Science and TechnologyWuhan 430030China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai 200032China

Objective Variations are present in common clinical practices regarding best practice in managing hyperkalaemia(HK),there is therefore a need to establish a multi-specialty approach to optimal renin angiotension-aldosterone system inhibitors(RAASi)usage and HK management in patients with chronic kidney disease(CKD)&heart failure(HF).This study aimed to establish a multi-speciality approach to the optimal use of RAASi and the management of HK in patients with CKD and *** A steering expert group of cardiology and nephrology experts across China were convened to discuss challenges to HK management through a nominal group *** group then created a list of 41 statements for a consensus questionnaire,which was distributed for a further survey in extended panel group of cardiologists and nephrologists across *** was assessed using a modified Delphi technique,with agreement defined as"strong"(≥75%and<90%)and"very strong"(≥90%).The steering group,data collection,and analysis were aided by an independent *** A total of 150 responses from 21 provinces across China were recruited in the *** were comprised of an even split(n=75,50%)between cardiologists and *** 41 statements achieved the 75%consensus agreement threshold,of which 27 statements attained very strong consensus(≥90%agreement)and 14 attained strong consensus(agreement between 75%and 90%).Conclusion Based on the agreement levels from respondents,the steering group agreed a set of recommendations intended to improve patient outcomes in the use of RAASi therapy and HK management in China.

关键词： Sardiorenal syndrome chronic kidney failure heart failure hyperkalemia multidisciplinary communication RAASi

The pseudospectrum and spectrum(in)stability of quantum corrected Schwarzschild black hole

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Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 55-75页

作者： Li-ming Cao Jia-Ning Chen Liang-bi Wu Libo Xie Yu-Sen Zhou School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUCASHangzhou 310024China Peng Huanwu Center for Fundamental Theory Hefei 230026China Interdisciplinary Center for Theoretical Study and Department of Modern Physics University of Science and Technology of ChinaHefei 230026China

In this study,we investigate the pseudospectrum and spectrum(in)stability of quantum corrected Schwarzschild black ***,we use the hyperboloidal framework to cast the quasinormal mode(QNM)problem into an eigenvalue problem associated with a non-selfadjoint operator,and then the spectrum and pseudospectrum are ***,the invariant subspace method is exploited to improve the computational efficiency for *** investigation into the spectrum(in)stability entails two main *** the one hand,we calculate the spectra of the quantum corrected black hole,then by the means of the migration ratio,the impact of the quantum correction effect on the Schwarzschild black hole has been *** results indicate that the so-called“migration ratio instability”will occur for small black holes with small angular momentum number *** the eikonal limit,the migration ratios remain the same for each *** the other hand,we study the spectrum(in)stability of the quantum corrected black hole by directly adding some particular perturbations into the effective potential,where perturbations are located at the event horizon and null infinity,*** are two interesting observations under the same perturbation energy ***,perturbations at infinity are more capable of generating spectrum instability than those at the event ***,we find that the peak distribution can lead to the instability of QNM spectrum more efficiently than the average distribution.

关键词： quasinormal modes pseudospectrum hyperboloidal coordinate

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CALD1 facilitates epithelial-mesenchymal transition progression in gastric cancer cells by modulating the PI3K-Akt pathway

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 1029-1045页

作者： Wen-Qian Ma ming-Chang Miao Ping-An Ding bi-Bo Tan Wen-Bo Liu Shuo Guo Li-Mian Er zhi-Dong Zhang Qun Zhao Department of Endoscopy The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050011Hebei ProvinceChina Hebei Key Laboratory of Precision Diagnosis and Comprehensive Treatment of Gastric Cancer Shijiazhuang 050011Hebei ProvinceChina Department of Radiotherapy The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050011Hebei ProvinceChina The Third Department of Surgery The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050011Hebei ProvinceChina

BACKGROUND CALD1 has been discovered to be abnormally expressed in a variety of malignant tumors,including gastric cancer(GC),and is associated with tumor progression and immune infiltration;however,the roles and mechanisms of CALD1 in epithe-lial-mesenchymal transition(EMT)in GC are *** To investigate the role and mechanism of CALD1 in GC progression,invasion,and *** In this study,the relationship between CALD1 and GC,as well as the possible network regulatory mechanisms of CALD1,was investigated by bioinformatics and validated by ***1-siRNA was synthesized and used to trans-fect GC *** activity was measured using the CCK-8 method,cell migration and invasive ability were measured using wound healing assay and Transwell assay,and the expression levels of relevant genes and proteins in each group of cells were measured using qRT-PCR and Western blot.A GC cell xenograft model RESULTS bioinformatics results showed that CALD1 was highly expressed in GC tissues,and CALD1 was significantly higher in EMT-type GC tissues than in tissues of other types of *** prognosis of patients with high expression of CALD1 was worse than that of patients with low expression,and a prognostic model was constructed and *** experimental results were consistent with the results of the bioinformatics *** expression level of CALD1 in GC cell lines was all higher than that in gastric epithelial cell line GES-1,with the strongest expression found in AGS and MKN45 *** activity was significantly reduced after CALD1-siRNA trans-fection of AGS and MKN45 *** ability of AGS and MKN45 cells to migrate and invade was reduced after CALD1-siRNA transfection,and the related mRNA and protein expression was *** to bioinfor-matics findings in GC samples,the CALD1 gene was significantly associated with the expression of members of the PI3K-AKT-mTOR signaling pathway as well as the EMT signaling pathway,and was closely rel

关键词： Gastric tumor CALD1 Epithelial-mesenchymal transition Gene disruption Invasion Migration bioinformatics

Microphysical Characteristics of Rainfall Based on Long-Term Observations with a 2DVD in Yangbajain,Tibet

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Advances in Atmospheric Sciences 2024年第9期41卷 1721-1734页

作者： ming LI Yongheng bi Yonghai SHEN Yinan WANG Ciren Nima Tianlu CHEN Daren LYU Key Laboratory for Cosmic Rays of the Ministry of Education Tibet UniversityLhasa 850000China Key Laboratory of Middle Atmosphere and Global Environment Observation(LAGEO) Institute of Atmospheric PhysicsChinese Academy of ScienceBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China

Raindrop size distribution(DSD)plays a crucial role in enhancing the accuracy of radar quantitative precipitation estimates in the Tibetan Plateau(TP).However,there is a notable scarcity of long-term,high-resolution observations in this *** address this issue,long-term observations from a two-dimensional video disdrometer(2DVD)were leveraged to refine the radar and satellite-based algorithms for quantifying precipitation in the hinterland of the *** was observed that weak precipitation(Rbits characteristics similar to maritime-like *** constrained relationships between the slopeΛand shapeμ,D_(m)and N_(w)of gamma DSDs were ***,we established a correlation between the equivalent diameter and drop axis ratio and found that raindrops on the TP attain a nearly spherical ***,the application of the rainfall retrieval algorithms of the dual-frequency precipitation radar in the TP is improved based on the statistical results of the DSD.

关键词： Tibetan Plateau raindrop size distribution 2DVD dual frequency radar microphysical features

Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

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Phyton-International Journal of Experimental Botany 2024年第5期93卷 1057-1066页

作者： Yulin Luo Panpan Yang Mengmeng bi Leifeng Xu Fang Du Jun ming College of Horticulture Shanxi Agricultural UniversityJinzhong030801China State Key Laboratory of Vegetable biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing100081China College of Urban and Rural Construction Shanxi Agricultural UniversityJinzhong030801China

The xylem undergoes physiological changes in response to various environmental conditions during the process of plant *** understand these physiological changes,it is extremely important to observe the transport of *** this study,the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin *** for introducing a fluorescent tracer into the xylem of the stems were ***,the transport rule of 5(6)-Carboxyfluorescein diacetate(CFDA)in the xylem of the stem of *** was studied by fluorescence dye in live cells tracer *** results showed that the vascular bundles of *** were scattered in the basic tissue,the peripheral vascular bundles were smaller and densely distributed,and the closer to the center,the larger the volume of vascular bundles and the more sparsely *** vascular bundles of *** are limited external tenacity vascular bundles,which are composed of phloem and *** most suitable method for CFDA labeling the xylem of isolated stem segments of *** was solution soaking for 24 *** running speed of CF in the isolated stem was 0.3 cm/h,which was consistent with the running speed of the material in the *** could be transported between the xylem and parenchyma cells,indicating that the material transport in the xylem could be through the symplastic *** above results laid a foundation for the study of the xylem transport mechanism and the xylem pathogen disease of lily.

关键词： Lilium xylem transport stem fluorescent dye CFDA