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Asian Pacific Journal of Tropical Medicine 2010年第5期3卷 356-359页

作者： Anindita Majumdar Pranita D Kamble CM Badole bc harinath Jamnalal Bajaj Tropical Disease Research Centre Department of Biochemistry MGIMSSevagramIndia Department of Orthopaedics MGIMSSevagramIndia

Objective:To evaluate inhouse developed SEVA TB peroxidase enzyme immunoassay using cocktail of mycobacterial excretory-secretory antigens(ES-31,ES-43 & EST-6) for antibody detection and their affinity purified antibodies for antigen detection in tuberculosis suspected ***:Inhouse developed SEVA TB peroxidase enzyme immunoassay was evaluated prospectively in 73 suspected pulmonary and 46 extra-pulmonary tuberculosis patients during November 2008~March 2009 in a tertiary hospital located in rural ***:Assay on prospective analysis showed 100% correlation of pulmonary tuberculosis(PTB) and extrapulmonary tuberculosis(EPTB) acid fast bacilli positivity and antitubercular treatment in 11 *** nine PTB and 12 EPTB cases showed negative for EUSA test and were also not given antitubercular *** 30 PTB and 27 EPTB cases showing ELISA positivity were neither acid fast bacilli positive nor antitubercular therapy *** cases may possibly have dormant infection and need further *** EPTB cases ELISA was observed to be more useful than AFB smear ***:This inhouse developed user-friendly peroxidase ELISA can be used as an adjunct test of smear microscopy or culture techniques for routine screening of patients suspected of PTB or EPTB.

关键词： Excretory secretory antigen Cocktail antigen SEVA TB ELISA Pulmonary tuberculosis Extra-pulmonary tuberculosis

Appendiceal inflammation affects the length of stay following appendicectomy amongst children:a myth or reality?

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Frontiers of Medicine 2013年第2期7卷 264-269页

作者： Khurram Siddique Shirin Mirza Gandra harinath Department of General Surgery East Kent Hospitals University NHS Foundation TrustKent CT13NGUnited Kingdom Public Health Solutions(Research&Development Organization) Islamabad 44000Pakistan

The effect of the severity of appendiceal inflammation on post-operative stay in children following appendicectomy has shown conflicting *** study was conducted to determine the association between the severity of appendiceal inflammation and post-operative stay amongst children undergoing open appendicectomy.A retrospective cohort study was conducted at a District General Hospital for two years.A total of 204 patients were included in the study with an age range between 3 and 16 *** were 54.9%while the rest were *** age was 12.5±3 *** association of the severity of appendiceal inflammation and post-operative stay was assessed by multivariable Cox Proportional hazards *** post-operative stay was 2.32 days(95%CI 2.14–2.51).Macroscopically perforated appendix,histological inflammation and post-operative complications were significantly associated with post-operative stay on univariable analysis(P<0.05).Whereas,the multivariable analysis showed that the post-operative stay was significantly prolonged only in case of either perforated appendix or post-operative complications while it remained unaffected by the histological inflammation.

关键词： appendiceal inflammation post-operative stay paediatrics

Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)

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The Crop Journal 2015年第1期3卷 46-56页

作者： Ashok A.Nikam Rachayya M. Devarumath Akash Ahuja harinath Babu Mahadeo G.Shitole Penna Suprasanna Tissue Culture Section Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Molecular Biology and Genetic Engineering Division Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Department of Botany Pune University Plant Stress Physiology & Biotechnology Section Nuclear Agriculture & Biotechnology Division Bhabha Atomic Research Centre

Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and K+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and K+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

关键词： Gamma-rays Mutagenesis Embryogenic callus Saccharum officinarum L Salt tolerance

Synthesis, antibacterial and anti-inflammatory activity of bis(indolyl)methanes

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Chinese Chemical Letters 2016年第1期27卷 16-20页

作者： Santhisudha Sarva Jayaprakash Soora harinath Siva Prasad Sthanikam Selvarajan Ethiraj Mohanasrinivasan Vaithiyalingam Suresh Reddy Cirandur Department of Chemistry Sri Venkateswara University School of Biosciences and Technology VIT University

A series of bioactive bis（indolyl）methanes are synthesized by one-pot green reaction of indole with various substituted aldehydes by microwave irradiation under solvent free conditions. The antibacterial activity against Staphylococcus aureus and anti-inflammatory activity of the synthesized bis（indolyl）-methanes are evaluated in vitro and compared to standard drugs tetracycline and diclofenac,respectively. The majority of the compounds showed good antibacterial and anti-inflammatory ***, compounds 3j, 3i, 3k and 3g exhibited much higher anti-inflammatory activity than the standard diclofenac drug and thus qualify for clinical trials to be used as an anti-inflammatory compound.

关键词： Bis(indolyl)methanes Microwave irradiation Antibacterial activity Anti-inflammatory activity

On Power Allocation for Transmitter and Receiver Selection in MIMO Fading Channels

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通讯和计算机（中英文版） 2011年第6期8卷 483-489页

作者： Shaik Qadeer Niranjan Kumar harinath Reddy Mohammed Zafar Ali Khan Dept. of Electrical Engg M.J. College of Engg. Osmania University Hyderabad AP-500034 India IIIT Hyderabad AP-500008 India Dept. of Electrical Engg. HT Hyderabad AP -502205 India

Magneto Convective Flow of a Non-Newtonian Fluid through Non-Homogeneous Porous Medium past a Vertical Porous Plate with Variable Suction

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Journal of Applied Mathematics and Physics 2016年第2期4卷 233-248页

作者： S. harinath Reddy M. C. Raju E. Keshava Reddy Department of Humanities and Sciences Annamacharya Institute of Technology and Sciences (Autonomous) Rajampet India Department of Mathematics Jawaharlal Nehru Technological University Hyderabad India

Radiation absorption and chemical reaction effects on unsteady MHD free convective flow of a viscoelastic fluid past a vertical porous plate in the presence of variable suction and heat source is considered. A uniform magnetic field is assumed to be applied in the transverse direction of the flow. The set of non-linear partial differential equations is transformed into a set of ordinary differential equations by super imposing a solution with steady and unsteady part. The set of ordinary differential equations is solved by using regular perturbation scheme. The expressions for velocity, temperature and species concentration fields are obtained and the expressions for Skin friction, Nusselt number and Sherwood number are also derived. The effects of numerous physical parameters on the above flow quantities are studied with the help of graphs and tables.

关键词： MHD Visco-Elastic Fluid Unsteady Flow Chemical Reaction Radiation Absorption Suction

Clinicopathologic diagnosis of dVIN related vulvar squamous cell carcinoma:An extended appraisal from a tertiary women's hospital

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Gynecology and Obstetrics Clinical Medicine 2023年第1期3卷 30-37页

作者： Tiannan Wang Vandana Baloda Lakshmi harinath Terrell Jones Huina Zhang Rohit Bhargava Chengquan Zhao Department of Pathology Magee-Womens HospitalUniversity of Pittsburgh Medical CenterPittsburghPA15213USA Department of Pathology Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCA90033USA Department of Pathology University of Rochester Medical CenterRochesterNY14642USA

Background:Differentiated vulvar intraepithelial neoplasia(dVIN)is a non-human papilloma virus(HPV)-related high-grade precursor lesion to vulvar squamous cell carcinoma(vSCCa).Although TP53 gene mutations have been identified in 80%of dVIN,its role in dVIN pathogenesis as well as malignant transformation is still being poorly *** reproducible diagnostic criteria and ambiguous p53 immunostaining patterns,along with morphologic discordance still pose a diagnostic ***:A series of 60 cases of dVIN-related vSCCa along with adjacent dVIN were *** features as well as immunohistochemical results were recorded on the resection-confirmed dVIN-related ***:The average age of the patients was 71 ***-five cases(58.4%)of dVIN-related vSCCa were moderately differentiated,fourteen cases(23.3%)were poorly differentiated,and the remaining eleven cases(18.3%)were ***-nine cases(48.3%)were found to have lichen sclerosus adjacent to *** terms of p53 and p16 expression in dVIN-related vSCCa and the adjacent dVIN,fifty-five(91.7%)dVIN showed mutant p53 immunostaining pattern with strong positive expression in 80%cases(basal/para-basal expression)and null pattern expression in 11.7%***(8.3%)dVIN showed p53 wild-type staining *** wild-type pattern were seen in 5%of vSCCa and p53 null pattern were seen in 13.3%*** cases demonstrated atypical staining patterns:two cases showed p53 null expression in dVIN but p53 overexpression in invasive carcinoma;three cases exhibited p53 null expression in invasive carcinoma,with the adjacent dVIN showing basal and para-basal mutant(2 cases)and wild-type(1 case)p53 expression patterns.A single case demonstrated p53 wild-type pattern in dVIN and overexpression in invasive *** addition,65%dVIN were p16 negative and 31.7%dVIN had patchy p16 ***:Clinical and prognostic value of the ambiguous/inconsistent patterns are u

关键词： Differentiated vulvar intraepithelial neoplasia Vulvar squamous cell carcinoma Clinicopathologic features Immunostaining patterns

应用单克隆抗体酶联免疫吸附试验双抗体法检测人体丝虫病循环抗原

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中国寄生虫学与寄生虫病杂志 1987年第1期 3-7页

作者：郑惠君,陶增厚,M.V.R.Reddy,B.C.harinath,W.F.Piessens 贵州省寄生虫病研究所贵州省寄生虫病研究所印度Mahatma Gandhi医学科学研究所生化研究室印度Mahatma Gandhi医学科学研究所生化研究室美国哈佛大学公共卫生学院热带病学教研组

应用兔抗微丝蚴抗体及抗班氏丝虫微丝蚴代谢抗原(ES34株)或抗马来丝虫3期幼虫抗原(HC11株)单克隆抗体(McAb)酶联免疫吸附试验(ELISA)双抗体法检测人体丝虫病循环抗原时,微丝蚴血症者阳性率分别为94.5％(103/109)及89.0％(97/109),且ES3... 详细信息

应用兔抗微丝蚴抗体及抗班氏丝虫微丝蚴代谢抗原(ES34株)或抗马来丝虫3期幼虫抗原(HC11株)单克隆抗体(McAb)酶联免疫吸附试验(ELISA)双抗体法检测人体丝虫病循环抗原时,微丝蚴血症者阳性率分别为94.5％(103/109)及89.0％(97/109),且ES34株单克隆抗体ELISA检测结果显示微丝蚴密度与抗原滴度呈正相关;部分微丝蚴血症者尿液中亦可测得丝虫抗原;晚期丝虫病人阳性率分别为57.4％(31/54)及61.1％(33/54);获自美国及中国贵阳非丝虫病流行区正常人血清阳性率分别为0～4.1％及2.8～4.1％;30份肠道蠕虫感染者血清全部为阴性。血清中丝虫循环抗原的存在似与活动性感染有关。

关键词：循环抗原双抗体法血清丝虫病蠕虫病微丝单克隆抗体