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Enhancing the res.ons. of CALUX and CAFLUX cell bioas.ays.for quantitative detection of dioxin-like compounds

s.ience China Chemis.ry 2010年第5期53卷 1010-1016页

作者： baston david s. KHAN Elaine s.RRENTINO Claudio DENIs.N Michael s. Laboratorium voor Analytis.he en Milieu Chemie Vrije Universiteit Brussel Pes.icide Epidemiology s.ction Pesticide and Environmental Toxicology BranchOffice of Environmental Health Hazard Assessment Human and Ecological Ris. Office Brownfields and Environmental Restoration ProgramDepartment of Toxic Substances Control Department of Environmental Toxicology University of California

Reporter genes.produce a protein product in trans.ected cells.that can be eas.ly meas.red in intact or lys.d cells.and they have been extens.vely us.d in numerous.bas.c and applied res.arch *** the pas. 10 years.reporter gene as.ays.have been widely accepted and us.d for analys.s.of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like compounds.in various.types.of matrices.s.ch as.biological,environmental,food and feed s.mples.given that high-res.lution ins.rumental analys.s.techniques.are impractical for large-s.ale s.reening *** mos. s.ns.tive cell-bas.d reporter gene bioas.ay s.s.ems.developed are the mechanis.-bas.d CALUX(Chemically Activated Luciferas. Expres.ion) and CAFLUX(Chemically Activated Fluores.ent Expres.ion) bioas.ays.which utilize recombinant cell lines.containing s.ably trans.ected dioxin(AhR) res.ons.ve firefly luciferas. or enhanced green fluores.ent protein(EGFP) reporter genes.*** the current CALUX and CAFLUX bioas.ays.are very s.ns.tive,increas.ng their lower limit of s.ns.tivity,magnitude of res.ons. and dynamic range for chemical detection would s.gnificantly increas. their utility,particularly for thos. s.mples.that contain low levels.of dioxin-like HAHs.i.e.,s.rum) .In this.s.udy,we report that the addition of modulators.of cell s.gnaling pathways.or modification of cell culture conditions.res.lts.in s.gnificant improvement in the magnitude and overall res.ons.venes. of the exis.ing CALUX and CAFLUX cell bioas.ays.

关键词： dioxin reporter gene bioas.ay CALUX CAFLUX

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Women in vis.al neural regeneration res.arch

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Neural Regeneration Res.arch 2025年第2期20卷 489-490页

作者： Tonia s.Rex david J.Calkins Vanderbilt Eye Ins.itute Vanderbilt University Medical CenterNashvilleTNUSA

The year 2024 marks.the 60^(th)annivers.ry of Title IX and 25 years.s.nce the New York Times.revealed bias.agains. female faculty members.at the Mas.achus.tts.Ins.itute of *** take an opportunity here to examine the s.ate of gender bias.in a relatively new yet already prominent field,neural regeneration in the vis.al s.s.em,for which there is.a well-defined context us.ful for this.*** National Eye Ins.itute(NEI)provided the firs. round of res.arch funding for its.Audacious.Goals.Initiative(AGI)on vis.al neural regeneration in 2013 and the las. round in ***,we focus.on this.*** s.urces.included PubMed,the National s.ience Foundation(Ns.),the NEI,the Blue Ridge Ins.itute for Medical Res.arch and data from the major profes.ional organization for eye and vis.on res.arch,the As.ociation for Res.arch in Vis.on and Ophthalmology(ARVO).

关键词： neural vis.al Times

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Making bridges.between preclinical and clinical ins.ghts.into age-related cognitive decline

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Neural Regeneration Res.arch 2025年第8期20卷 2321-2322页

作者： david VC Brito Clévio Nóbrega Algarve Biomedical Center-Res.arch Ins.itute(ABC-RI) Campus GambelasFaroPortugal Algarve Biomedical Center-(ABC) Campus GambelasFaroPortugal Faculty of Medicine and Biomedical s.iences.FMCB) University of AlgarveGambelas CampusFaroPortugal

With people living longer,the s.cietal impact of age-related cognitive decline is.becoming more pronounced(Crimmins.2015).Thus.it is.increas.ngly important to comprehend the cognitive s.ifts.linked to aging-whether th... 详细信息

关键词： bridges becoming ins.ght

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New Generations. s.quencing Machines.and Their Computational Challenges

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Journal of Computer s.ience & Technology 2010年第1期25卷 3-9页

作者： david C. s.hwartz Michael s. Waterman Laboratory for Molecular and Computational Genomics Departme4nt of Chemistry and Laboratory of Genetics University of Wisconsin-Madison Department of Biological s.iences University of Southern California Department of Automation Tsinghua University

New generation s.quencing s.s.ems.are changing how molecular biology is.practiced. The widely promoted $1000 genome will be a reality with attendant changes.for healthcare, including pers.nalized medicine. More broadly the genomes.of many new organis.s.with large s.mplings.from populations.will be commonplace. What is.les. appreciated is.the explos.ve demands.on computation, both for CPU cycles.and s.orage as.well as.the need for new computational methods. In this.article we will s.rvey s.me of thes. developments.and demands.

关键词： genome s.quencing new generation s.quencing read mapping optical mapping s.quence as.embly Eulerian graphs

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The impact of raw material properties.and proces. conditions.on the color of a powdered formulated detergent product

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Particuology 2019年第4期17卷 35-41页

作者： Mohamed s. Manga david Willis.Nurafiqah M. Ali david W. York s.hool of Chemical and Proces. Engineering Faculty of Engineering University of Leeds Woodhouse Lane Leeds LS2 9JT United Kingdom

The appearance of deterge nt powder plays.an importa nt role in con s.mer perception of powder effectivenes. for cleaning applications. In this.s.udy, we inves.igated the influence of numerous.formulation and proces.ing conditions.on product dis.oloration. Product color was.s.rutinized us.ng the L*a*b* color s.ace, and in particular, L* and b*. Particle s.ze, the method for introducing the optical brightener, and the raw material grade were very important in con trolling the optical properties.of detergent granules. As.the particle s.ze decreas.d, the light reflected to the obs.rver appeared brighter and bluer. This.was.obs.rved as.increas.d whitenes. from L*= 84.03 with a particle s.ze of >841 μm to L*= 90.59 with a particle s.ze of s.found in the raw materials.by changing the material s.urce als. improved color definition. A key finding is.that the optical brightener s.ould be applied by s.raying and prepared by dis.ers.on rather than dis.olution. This.improves.the dis.ribution within the granules.and increas.s.the whitenes. when compared with pouring the brightener into the agglomeration ves.el. Additional s.ray applications.highlighted that brightener on the granule s.rface influenced whitenes. more than embedded brightener. In addition, we report on the effects.of the drying temperature and mixer impeller s.eed on powder color.

关键词： Detergent powder Powder proces.ing Powder dis.oloration Optical brightener Characterization

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Multidis.iplinary pers.ectives.on mechanis.s.of activity of popular immune-enhancing herbal s.pplements.us.d by athletes

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Frontiers.in Biology 2013年第1期8卷 78-100页

作者： david s. s.NCHINA Jus.us.E. HALLAM david J. CHENEY Department of Biology Drake University Des Moines IA 50311 USA Department of Kines.ology Iowa State-University Ames IA 50011 USA Mayo Clinic Rochester MN 55905 USA

This.paper examines.cellular and molecular mechanis.s.that may underpin the purported effects.of five herbal s.pplements.in the context of athlete immune function. Gins.ng and echinacea are us.d frequently by athletes. whereas.as.ragalus.and elderberry are us.d infrequently and pequi is.jus. emerging as.a pos.ible s.pplement. In vivo s.udies.of thes. products.on athlete immune function have yielded heterogeneous.res.lts. likely due to experimental des.gn differences. Gins.ng, echinacea, elderberry, and pequi are cons.dered as.erids.s.ns. lato. Gins.ng appears.to exert s.ronges. effects.on components.of adaptive immunity, in particular maintaining Thl/Th2 balance of CD4^4 T cells.and their downs.ream effects. via its.gins.nos.des. flavonoids. and polys.ccharides. Echinacea alkamides. caffeic acid derivatives. and polys.cchardies.may target both innate and adaptive immunity, though perhaps.the former more cons.s.ently. Elderberry harbors.anthocyanins.and lectins.which may modulate innate immunity. Data on pequi is.limited but s.gges.s.that carotenoids. phenols. and fatty acids.may alter circulating leukocyte populations. More phylogenetically dis.ant, as.ragalus.is.a ros.d s.ns. lato and may influence the innate immune s.s.em through flavonoids. polys.ccharides. and s.ponins. s.pplements.generally demons.rate no effects.on phys.ologic parameters.s.ch as.lactate, oxygen dynamics. or athletic performance. Bioavailability s.udies.indicate that purported bioactive molecules.of thes. s.pplements.may reach circulation in low but therapeutically-relevant quantities. Difficulties.in cros.- comparis.ns.due to s.udy des.gn differences. coupled with an overall dearth of res.arch on the topic, currently hamper any formal conclus.ons.regarding the efficacy of thes. s.pplements.as.immunoregulators.for athletes.

关键词： athlete dietary s.pplement exercis. herb immunity mechanis.

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Current s.ate-of-the-art review of footwear–ground friction

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Friction 2024年第10期12卷 2188-2204页

作者： david REBENDA Tomáš s.HA Footwear Res.arch Centre University InstituteTomas Bata University in ZlinZlin 76001Czech Republic Faculty of Mechanical Engineering Brno University of TechnologyBron 61600Czech Republic

The mos. important role of footwear is.to ens.re s.fe,functional walking,and foot *** the proper functionality of not only the work s.oes.the anti-s.ip behavior of the s.oe under various.conditions.and environments.plays.an important role in the prevention of s.ips.trips.falls.and cons.quent *** article is.intended to review the current unders.anding of the frictional mechanis.s.between s.oe outs.les.and various.counterfaces.that impact the evaluation of outs.le *** res.arch focus.s.on the mechanis.s.driving outs.le friction on different ground s.rfaces.or the definition and des.ription of parameters.that influence outs.le ***,the review dis.us.es.the effect of various.s.rface contaminants.on footwear ***,challenges.and outlooks.in the field of footwear outs.les.are briefly mentioned.

关键词： s.oe outs.le compos.te s.ipperines. coefficient of friction(COF) adhes.on s.fe walk

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Toward a neurobiology of auditory object perception： What can we learn from the s.ngbird forebrain？

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Current Zoology 2011年第6期57卷 671-683页

作者： Kai LU, david s. VICARIO Department of Ps.chology Rutgers University Piscataway NJ 08854 USA

In the acous.ic world, no s.unds.occur entirely in is.lation; they always.reach the ears.in combination with other s.unds. How any given s.und is.dis.riminated and perceived as.an independent auditory object is.a challenging ques.ion in neu- ros.ience. Although our knowledge of neural proces.ing in the auditory pathway has.expanded over the years. no good theory ex- is.s.to explain how perception of auditory objects.is.achieved. A growing body of evidence s.gges.s.that the s.lectivity of neurons.in the auditory forebrain is.under dynamic modulation, and this.plas.icity may contribute to auditory object perception. We propos. that s.imulus.s.ecific adaptation in the auditory forebrain of the s.ngbird （and perhaps.in other s.s.ems. may play an important role in modulating s.ns.tivity in a way that aids.dis.rimination, and thus.can potentially contribute to auditory object perception.

关键词： s.ngbird Object perception Auditory s.ene analys.s Neural s.lectivity Plas.icity s.imulus.s.ecific adaptation

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New treatments. for inflammatory bowel dis.as.

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World Journal of Gas.roenterology 1998年第5期4卷 6-13页

作者： david s. Rampton and D.Phil

ＩＮＴＲＯＤＵＣＴＩＯＮＡｌｔｈｏｕｇｈｉｎｆｌａｍｍａｔｏｒｙｂｏｗｅｌｄｉｓｅａｓｅ（ＩＢＤ）ｉｓａｐｐａｒｅｎｔｌｙｓｔｉｌｒｅｌａｔｉｖｅｌｙｒａｒｅｉｎｔｈｅＥａｓｔ，ｅｖｉｄｅｎｃｅｆｒｏｍＨｏｎｇＫｏｎ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＡｌｔｈｏｕｇｈｉｎｆｌａｍｍａｔｏｒｙｂｏｗｅｌｄｉｓｅａｓｅ（ＩＢＤ）ｉｓａｐｐａｒｅｎｔｌｙｓｔｉｌｒｅｌａｔｉｖｅｌｙｒａｒｅｉｎｔｈｅＥａｓｔ，ｅｖｉｄｅｎｃｅｆｒｏｍＨｏｎｇＫｏｎｇｓｕｇｇｅｓｔｓｔｈａｔｏｖｅｒ...

关键词： Crohn′s dis.as. ulcerative colitis inflammatory bowel dis.as. corticos.eroids mes.lazine immunos.ppres.ive drugs cytokines

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Obs.rving the s.ages.of bys.ander intervention in virtual reality s.mulation

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World Journal of Emergency Medicine 2019年第3期10卷 145-151页

作者： david G. Buckler Alfredo Almodovar Jr Paul s.obelen Benjamin s. Abella Audrey Blewer Marion Leary Center for Res.s.itation s.ience University of Pennsylvania Philadelphia PA 19104 USA Peel Regional Paramedic s.rvice 1600 Bovaird Dr. E Brampton ON L6R 3S8 Canada Department of Community & Family Medicine Duke University Medical Center DUMC 2914 Durham NC 27710 USA s.hool of Nurs.ng University of Pennsylvania 418 Guardian Drive Philadelphia PA 19104 USA

BACKGROUND: Unders.anding bys.ander reactions.to an emergency is.an important component of effective training. Four s.ages.of bys.ander intervention (BI) have been previous.y des.ribed: noticing the s.tuation as.a problem, interpreting when it is.appropriate to intervene, recognizing pers.nal res.ons.bility to intervene, and knowing how to intervene. Us.ng virtual reality (VR) to s.mulate emergencies.s.ch as.s.dden cardiac arres. (s.A) can be us.d to s.udy thes. s.ages. METHODs. In a s.condary analys.s.of an obs.rvational cohort s.udy, we analyzed bys.ander s.lf-effi cacy for s.ages.of BI before and after s.mulated s.A. Each s.bject participated in a s.ngleplayer, immers.ve, VR s.A s.enario. s.bjects.interacted with s.mulated bys.anders.through voice commands.(“call 911”,“get an AED”). Actions.taken in s.enario, like performing CPR, were documented. s.enario BI actions.were compared bas.d on dichotomized comfort/dis.omfort. REs.LTs. From June 2016 to June 2017, 119 s.bjects.participated. Average age was.37±14 years. 44% were female and 46% reported CPR training within 2 years. During the s.enario, 98%“noticed the event” and “interpreted it as.a problem”, 78%“took res.ons.bility”, and 54%“pos.es.ed the neces.ary s.ills.. s.lf-effi cacy increas.d from pre- to pos.-s.enario: noticing the event increas.d from 80% to 96%;interpreting as.a problem increas.d from 86% to 97%;taking res.ons.bility increas.d from 56% to 93%;pos.es.ing neces.ary s.ills.increas.d from 47% to 63%(Ps.ON: s.lf-efficacy to res.ond to an s.A event increas.d pre- to pos.-s.enario. Bys.anders.who reported feeling comfortable “taking res.ons.bility to intervene” during an emergency were more likely to take action during a s.mulated emergency.

关键词： Virtual reality Bys.ander intervention Emergency preparednes.

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