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The Up-conversion Laser Properties of a Non-polar Organic Dye and the Influence of Solvents and Pumping Wavelengths

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Chinese Chemical Letters 2006年第1期17卷 81-84页

作者： Xiao Qiang YU Xian ZHANG Gui bao XU hua Ping zhao Wei HE Zong Shu SHAO Xian Gang XU Min hua JIANG The National Key Lab of Crystal Materials Shandong University Jinan 250100

A non-polar organic dye, E, E-1, 4-bis[4＇-（N,N-dibutylamino）styryl]-2,5-dimethoxybenzene （DBASDMB）, has been synthesized and characterized, and its structure has been determined. Pumped with a 200fs pulse this dye showed the up-conversion laser properties. The influences of various organic solvents and different pumping wavelength on the laser properties have been demonstrated.

关键词： Up-conversion laser two-photon absorption non-polar organic dye.

Cloning of α-β fusion gene from Clostridium perfringens and its expression

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World Journal of Gastroenterology 2006年第8期12卷 1229-1234页

作者： Jia-Ning Bai Yan Zhang bao-hua zhao College of Life Science Hebei Normal University Shijiazhuang 050016 HebeiProvince China

AIM： To study the cloning of α-β fusion gene from Closindium perfringens and the immunogenidty of 0-6 fusion expression. METHODS： Cloning was accomplished after PCR amplification from strains NCTC64609 and C58-1 of the protective antigen genes of α-toxin and β-toxin. The fragment of the gene was cloned using plasmid pZCPAB. This fragment coded for the gene with the stable expression of α-β fusion gene binding. In order to verify the exact location of the α-β fusion gene, domain plasmids were constructed. The two genes were fused into expression vector pBV221. The expressed α-β fusion protein was identified by ELISA, SDS-PAGE, Western blotting and neutralization assay. RESULTS： The protective co-toxin gene （cpa906） and the β-toxin gene （cpb930） were obtained. The recombinant plasmid pZCPAB carrying α-β fusion gene was constructed and transformed into BL21（DE3）. The recombinant strain BL21（DE3）（pZCPAB） was obtained. After the recombinant strain BL21（DE3）（pZCPAB） was induced by 42℃, its expressed product was about 22.14% of total cellular protein at SDS-PAGE and thin-layer gel scanning analysis. Neutralization assay indicated that the antibody induced by immunization with α-βfusion protein could neutralize the toxicity of α-toxin and β-toxin. CONCLUSION： The obtained α-toxin and β-toxin genes are correct. The recombinant strain BL21（DE3）（pZCPAB） could produce α-β fusion protein. This protein can be used for immunization and is immunogenic. The antibody induced by immunization with α-β fusion protein could neutralize the toxicity of α-toxin and β-toxin.

关键词： Clostridium perfringens α-β fusion gene Cloning and expression

Studies on the Chemical Constituents of Lysimachia capillipes Hemsl

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Chinese Chemical Letters 1998年第12期9卷 0-0页

作者： Chen XIE Li Zhen XU bao hua zhao and Shi Lin YANG(Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and PekingUnion Medical College, Beijing 100094) Chinese Acad Med Sci Inst Med Plant Dev Beijing 100094 Peoples R China Peking Union Med Coll Beijing 100094 Peoples R China

Eight compounds were isolated from the ethanolic extracts of Lysimachia capillipes Hemsl. They were identified as capilliplactone 1, capillipnin 2,beta-daucosterol 3, succinic acid 4, quercetin 5, 3',4',5,5',7-pentahydroxyflavone 6, quercetin-3-O-beta-D-glucopyranoside 7 and kaempferol 8 on the basis of chemical and spectral methods. Among them, 1 and 2 were new, their structures were established as all-cis-5-deoxy-pentonic acid-gamma-lactone and 1,2,4,5 -tetrahydroxy-3-n-pentylbenzen-2,4-di-O-beta-D-glucopyranoside through their spectral data ((HNMR)-H-1, (CNMR)-C-13, DEFT, H-1-H-1 COSY, H-1-C-13 COSY, HMBC etc).

关键词： Lysimachia lysimachia capillipes, capillplactone capillipnin

A Cell-based High-throughput Screening Assay for Farnesoid X Receptor Agonists

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Biomedical and Environmental Sciences 2007年第6期20卷 465-469页

作者： ZHI-HUI ZHENG Guo-PING LV SHU-YI SI YUE-SHENG DONG bao-hua zhao hua ZHANG JIAN-GONG HE Insitute of Medicinal Biotechnology Peking Union Medical College / Chinese Academy of Medical Sciences Beijing 100050 China College of Life Science Hebei Normal University Shijiazhuang 050016 Hebei China New Drug Research ＆ Development Center of North China Pharmaceutical Group Corporation National Microbial Medicine Engineering ＆ Research Center Shijiazhuang 050015 Hebei China

Objective To develop a high-throughput screening assay for Farnesoid X receptor （FXR） agonists based on mammalian one-hybrid system （a chimera receptor gene system） for the purpose of identifying new lead compounds for dyslipidaemia drug from the chemical library. Methods cDNA encoding the human FXR ligand binding domain （LBD） was amplified by RT-PCR from a human liver total mRNA and fused to the DNA binding domain （DBD） of yeast GAL4 of pBIND to construct a GAL4-FXR （LBD） chimera expression plasmid. Five copies of the GAL4 DNA binding site were synthesized and inserted into upstream of the SV40 promoter of pGL3-promoter vector to construct a reporter plasmid pG5-SV40 Luc. The assay was developed by transient co-transfection with pG5-SV40 Luc reporter plasmid and pBIND-FXR-LBD （189-472） chimera expression plasmid. Results After optimization, CDCA, a FXR natural agonist, could induce expression of the luciferase gene in a dose-dependent manner, and had a signal/noise ratio of 10 and Z＇ factor value of 0.65, Conclusion A stable and sensitive cell-based high-throughput screening model can be used in high-throughput screening for FXR agonists from the synthetic and natural compound library.

关键词： Farnesoid X receptor Agonist High-throughput screening Chimera

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幽门螺杆菌感染与糖尿病

世界华人消化杂志 2016年第8期24卷 1220-1226页

作者：宋淑然赵丽梅赵宝华河北医科大学第二医院检验科河北省石家庄市050000 河北医科大学第二医院消化内科河北省石家庄市050000 河北师范大学生命科学学院河北省石家庄市050024

糖尿病患者幽门螺杆菌(Helicobacter pylori,***)感染率明显增加,***感染作为一个独立因素可促使胰岛素抵抗.糖尿病患者中的***根除率较正常人为低,合并***感染的糖尿病患者血糖波动范围增大,血糖不易被控制,治疗效果不佳,而根除***后... 详细信息

糖尿病患者幽门螺杆菌(Helicobacter pylori,***)感染率明显增加,***感染作为一个独立因素可促使胰岛素抵抗.糖尿病患者中的***根除率较正常人为低,合并***感染的糖尿病患者血糖波动范围增大,血糖不易被控制,治疗效果不佳,而根除***后有助于改善糖尿病的进展.***感染与糖尿病并发症(如糖尿病肾病、动脉粥样硬化、胃轻瘫等)的发生也有一定关系.***感染影响糖尿病的机制可能与系统性炎症反应、血管内皮损伤和激素水平改变(如瘦素和胃饥饿素)等因素有关.

关键词：幽门螺杆菌糖尿病高血糖胰岛素抵抗

艾司西酞普兰与文拉法辛治疗老年焦虑症的疗效分析

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兰州大学学报（医学版） 2011年第3期37卷 37-39,44页

作者：包炤华无锡市精神卫生中心临床心理科江苏无锡214151

目的探讨艾司西酞普兰与文拉法辛治疗老年焦虑症患者的疗效和安全性。方法将64例符合中国精神障碍分类和诊断标准第3版焦虑发作的老年患者随机分为艾司西酞普兰组和文拉法辛组。艾司西酞普兰剂量为10～20mg/d,文拉法辛剂量为150～225mg/... 详细信息

目的探讨艾司西酞普兰与文拉法辛治疗老年焦虑症患者的疗效和安全性。方法将64例符合中国精神障碍分类和诊断标准第3版焦虑发作的老年患者随机分为艾司西酞普兰组和文拉法辛组。艾司西酞普兰剂量为10～20mg/d,文拉法辛剂量为150～225mg/d。疗效采用汉密尔顿焦虑量表(HAMA),不良反应和安全性用副反应量表和实验室检查评定,观察时间为6周。结果艾司西酞普兰组HAMA评分从第1周开始显著下降,文拉法辛组HAMA评分从第2周开始显著下降;艾司西酞普兰组有效率为84.3%,显效率为68.8%,文拉法辛组有效率为81.3%,显效率为64.0%。两组主要不良反应为恶心、口干、困倦、头晕等,其中文拉法辛组的恶心明显高于艾司西酞普兰组。结论艾司西酞普兰治疗老年焦虑症患者安全有效,疗效与文拉法辛相当,但起效快于文拉法辛。

关键词：艾司西酞普兰文拉法辛老年焦虑症

依据Tomita评分的后凸成形术治疗脊柱转移瘤

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中国矫形外科杂志 2018年第23期26卷 2113-2117页

作者：何文野包肇华季一鸣苏州大学附属第一医院江苏省苏州市215006

[目的]评价球囊扩张椎体后凸成形术在治疗Tomita评分4～7分脊柱转移性肿瘤患者的安全性和疗效。[方法]回顾2014年1月～2017年7月的脊柱转移瘤患者,对其进行Tomita评分,选择评分在4～7分的患者31例共65个椎体,对其进行经皮球囊扩张椎体... 详细信息

[目的]评价球囊扩张椎体后凸成形术在治疗Tomita评分4～7分脊柱转移性肿瘤患者的安全性和疗效。[方法]回顾2014年1月～2017年7月的脊柱转移瘤患者,对其进行Tomita评分,选择评分在4～7分的患者31例共65个椎体,对其进行经皮球囊扩张椎体后凸成形术。比较手术前后患者的VAS评分和ODI指数,采用WHO疼痛缓解标准评价随访时症状改善,拍摄X线片,测量比较后凸畸形角(Cobb角)、椎体前缘、中部高度。[结果]所有患者均顺利完成手术,无严重并发症。除1例患者死亡外,30例患者随访12～25个月,平均(17.83±2.86)个月。与术前相比,术后VAS评分、ODI指数显著减少,差异有统计学意义(P0.05)。[结论]对于Tomita评分4～7分的患者应用球囊扩张椎体后凸成形术,能迅速缓解胸腰椎转移瘤引起的疼痛,有效恢复病椎高度,提高生存质量。

关键词：肿瘤转移脊柱病理骨折后凸成形术

Rapid and scalable preparation of highly quaternized polymer networks to achieve efficient simultaneous adsorption of iodide and pertechnetate

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Science China Chemistry 2025年第01期 337-349页

作者： Meiyun Xu Song Gu Fulong Chen Zhe zhao Peng Liu Daoben hua State Key Laboratory of Radiation Medicine and Protection School of Radiological and Interdisciplinary Sciences (RAD-X) Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University

The efficient separation of fission products,such as TcO4-and I-,holds strategic significance for the management of radioactive wastes and environmental *** this study,we propose an ultrafast strategy for scalable preparation of a highly quaternized organic network to facilitate efficient and synchronous separation of iodide and *** network can be prepared within a few minutes at room temperature,using polyethylenimine and 1,2,4,5-tetrakis（bromomethyl）benzene as building *** chlorine replacement,the network,abundantly decorated with quaternary ammonium groups （Cl@QPN）,exhibits an ultrahigh positive charge density of 7.6 mmol/*** enables the rapid and efficient enrichment of target anions through strong electrostatic Coulomb *** a result,Cl@QPN exhibits significantly higher adsorption rate constants of 0.830 g/（mg min） for ReO4-（a nonradioactive surrogate of TcO4-） and 0.677 g/（mg min） for I-compared to other ***,it possesses high adsorption capacities,reaching 1,681 mg/g for ReO4-and 917.4 mg/g for I-.Cl@QPN also demonstrates good selectivity towards target ions and shows efficient adsorption for99TcO4-.Additionally,Cl@QPN exhibits high dynamic processing capacities,handling up to 3,100 and 7,400 kg of simulated streams per kilogram of material for I-and ReO4-,respectively.

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无神经症状单节段胸腰椎爆裂型骨折行后路短节段椎弓根螺钉内固定置入186例

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中国组织工程研究与临床康复 2010年第17期14卷 3185-3188页

作者：王骏骅杨惠林耿德春陈亮包肇华梅盺苏州大学附属第一医院骨科江苏省苏州市215006

背景:胸腰椎骨折治疗方法众多,但是对于无神经症状的爆裂型骨折,采用简单的手术方法治疗,是否在减少创伤的同时能够取得较好的疗效,从而避免手术的扩大化尚不清楚。目的:观察采用单纯后路短节段椎弓根螺钉系统置入治疗无神经症状的单节... 详细信息

背景:胸腰椎骨折治疗方法众多,但是对于无神经症状的爆裂型骨折,采用简单的手术方法治疗,是否在减少创伤的同时能够取得较好的疗效,从而避免手术的扩大化尚不清楚。目的:观察采用单纯后路短节段椎弓根螺钉系统置入治疗无神经症状的单节段胸腰椎爆裂型骨折的疗效。方法:选择2003-09/2008-01苏州大学附属第一医院骨科收治的无神经症状单节段胸腰椎爆裂型骨折患者186例,男152例,女34例,年龄18～65岁。均采用单纯后路短节段椎弓根螺钉内固定置入治疗,椎管内骨块占位采用间接复位。于置入前、置入后及置入后1年余取内固定前摄以伤椎为中心的X射线正侧位片和CT,对X射线平片和CT进行测量,统计伤椎前缘高度、伤椎横截面积内骨块的占有率。结果与结论:伤椎前缘置入前高度平均为正常的42%,术后为98%,内固定取出术前伤椎前缘高度为正常高度的98%。伤椎横截面积内骨块占有率,术前平均为34%,术后平均为13%,内固定取出术前为8%。提示应用后路短节段椎弓根螺钉系统治疗无神经症状的胸腰椎爆裂型骨折,能够提供脊柱足够的稳定性,有效恢复椎体高度、生理弧度和椎管容积。

关键词：胸腰椎骨折椎弓根内固定置入硬组织植入物