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基于改进的MobilenetV3热轧钢带表面缺陷分类

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兰州大学学报（自然科学版） 2024年第2期60卷 182-186页

作者：熊政车文刚保永莉刘晓彤昆明理工大学信息工程与自动化学院云南省计算机技术应用重点实验室昆明650500 昆明理工大学生命科学与技术学院昆明650500

提出一种基于轻量化神经网络MobilenetV3-large改进的热轧钢带表面缺陷分类算法,通过剪枝、大量削减卷积层数、调整通道大小和步长,以及修改对应的网络参数快速降低了参数量.为弥补修改模型带来的准确率下降的问题,将激活函数ReLU更换为... 详细信息

提出一种基于轻量化神经网络MobilenetV3-large改进的热轧钢带表面缺陷分类算法,通过剪枝、大量削减卷积层数、调整通道大小和步长,以及修改对应的网络参数快速降低了参数量.为弥补修改模型带来的准确率下降的问题,将激活函数ReLU更换为Hard-Swish,引入置换注意力机制替换原模型中的通道注意力机制,在进一步降低参数量的同时提高运行效率和分类准确率.在NEU-CLS表面缺陷数据集中的试验结果表明,改进后的算法参数量为0.5 MB,相比原模型降低96.89%,训练图片的时间由19.81 ms/幅降至10.73 ms/幅,平均准确率为99.26%,比改进前提高了5.56%,表明改进后的算法可应用于实时分类.

关键词： MobilenetV3算法转移注意力结构性剪枝缺陷分类

Components of Essential Oils in Different Parts of Daucus carota L. var. sativa Hoffm

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Chemical Research in Chinese Universities 2006年第3期22卷 328-334页

作者： WU Yin XU Ze-li li Hong-jun MENG Xiang-ying bao yong-li li Yu-xin Research Center of Agriculture and Medicine C ene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

The components of the essential oils obtained from different parts of Daacus carota ***. saliva Hoffm were analyzed. The percentages of the essential oils extracted are 0. 27% （ mL/100 g material） for the flowers, 0. 0/% for the stems and leaves and 0. 01% for the roots. Fifty-four, Sixty-six and Thirty-three compounds were,respectively, separated and identified from the flowers, the stems and leaves and the roots, among which unsaturated alkene compounds are thirty-nine, thirty-nine and twenty-one, respectively, accounting in turn for up to 90. 21%, 90. 49% and 72. 65% of the total essential oils. Because alkene compounds have double bonds that are easily oxidized, it can he inferred that the components of the essential oils in the different parts of Daucus carota L. var. saliva Hoffm should show an activity of the antioformation of free radicals to some extent.

关键词： Daucus carota L. var. sativa Hoffm Essential oil. GC/MS

Synergistic Effects of Activin A and Fibroblast Growth Factor 2 in the Modulation of Insulin Expression

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Chemical Research in Chinese Universities 2006年第2期22卷 229-232页

作者： bao yong-li JIANG Hong-yu Ji Shou-xian WU Yin MENG Xiang-ying li Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China The First Affiliated Hospital Jilin University Changchun 130021 P. R. China The Second Affiliated Hospital Jilin University Changchun 130041 P. R. China

Diabetes is the most prevalent and serious metabolic disease, and the number of diabetic patients worldwide is increasing. The reduction of insulin biosynthes is in pancreatic E-cells is closely associated with the onset and progression of diabetes, therefore, it is important to search for ways to induce insulin-producing cells in non-E-cells. In the present study, it has been reported that activin A and a basic fibroblast growth factor 2 （ FGF2）, can synergistically increase the insulin mRNA level, in both mouse El4 striatal primary cell cultures and the hippocampal neuronal cell line HT22. Activin A and FGF2 can jointly stimulate the nuclear translocation of Smad3 and specifically activate ERK1/2. It is interesting to note that a specific inhibitor for MEK, U0126, can efficiently block the induction of an insulin promoter activity by activin A and FGF2. This indicates that activin A collaborates with FGF2, giving a signal to induce the insulin gene through selective activation of the ERK-type MAP kinase and Smad3 in mouse striatal and HT22 cells. These data suggest that activin A may act in concert with FGF2 for the development of insulin -positive neurons

关键词： Activin A Fibroblast growth factor 2 (FGF2) Insulin

Analysis of Essential Oils from Flower-buds, Leaves and Stems of Filipendula palmata(Pall.) Maxim.

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Chemical Research in Chinese Universities 2005年第6期21卷 658-662页

作者： WU Yin MENG Xiang-ying liU Xiao-hua bao yong-li WANG Shu-ping li Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

The essential oils of the flower-buds, leaves and stems of Filipendula palmata（ Pall. ） Maxim. were analyzed by GC and GC-MS. Thirty-three, forty-seven and forty-seven compounds in the flower-buds, leaves and stems were identified, respectively. Methyl salicylate exists in a great amount which is up to 70. 10% in the flower-buds. Its amount is also high in other two parts. The data obtained show that it may be one of the main natural mosquito-expelling and pain-alleviating components in the three parts. The flower-buds are the main active part with the mosquito-expelling function.

关键词： Filipendula palmata( Pall. ) Maxim. Essential oil GC-MS

Construction and Characterization of a cDNA Expression library for the North American Ginseng Root Tissues

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Chemical Research in Chinese Universities 2007年第3期23卷 310-313页

作者： WANG Ying WANG Kun bao yong-li WU Yin MENG Xiang-ying li Yu-xin Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

The root of Panax ginseng plant undergoes a specific developmental process to become a biosynthesis and accumulation tissue for ginsenosides. To identify and analyze genes involved in the biosynthesis of ginsenoside, we constructed and characterized a full-length cDNA library for 6-year-old North American ginseng. The titer of primary cDNA library is 1.2 × 10^6 pfu/mL, the titer of amplified library is 2. 6 × 10^10 pfu/mL and the rate of recombinant is above 86%. The insert size ranges from 0. 3 to 2.0 kb. Sequencing results show that 18 of 58 genes are high homologous to the genes （GBRS, GBR3 and GBR1 ） known in GenBank, which are involved in biosynthesis of ginsenoside in North American ginseng plant; 16 of 58 genes are novel genes. The full-length cDNA library of North American ginseng root tissues is essential for the cloning of genes known and it is also an initial key for the screening and cloning of new genes.

关键词： cDNA library North American ginseng SMART

Isolation and Characterization of Proteins Interacting with Activin Type Ⅱ Receptors

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Chemical Research in Chinese Universities 2006年第2期22卷 217-220页

作者： liU Biao bao yong-li WEI Zhuang WU Yin MENG Xiang-ying li Yu-xin YIN Wei-tian Department of Hand Surgery China-japan Union Hospital Jilin University Changchun 130031 P. R. China Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

Regulation of the number of aetivin receptors that are present in the cell membrane plays a key role in the modulation of cellular responses to activin. In order to find the regulators, a novel protein ARIPzip, interacting with activin type II receptors, was searched and identified by using yeast two-hybrid screening. ARIPzip is a splicing variant of ARIP2. This has been discussed previously. ARIPzip can specifically interact with ActR Ⅱ A, and is widely distributed in mouse tissues. Overexpression of ARIPzip can cause the activin-induced transcriptional activities to increase in a dose-dependent manner while the overexpression of ARIV2 can decrease these activities. These data suggest that the C-terminal rezions of ARIP2 and ARIPzip are involved in the regulation of activin signaling.

关键词： Activin Activin receptor Ⅱ A( ActR Ⅱ A) Activin receptor interaction protein(ARIP)

Immunomodulatory effects of flazin from Crassostrea sikamea on splenic lymphocytes of Sprague-Dawley rats

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Chinese Journal of Natural Medicines 2021年第11期19卷 836-843页

作者： KONG Ying WANG li-Hua liU Lei ZHENG li-Hua bao yong-li liU Xiu-Xian WANG Shu-Yue SONG Zhen-Bo National Engineering Laboratory for Druggable Gene and Protein Screening School of Life SciencesNortheast Normal UniversityChangchun 130024China NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products Northeast Normal UniversityChangchun 130117China School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghai 201203China

Crassostrea sikamea(***)is an important edible and medicinal seafood in *** the present study,a compound named flazin was separated and identified from the ethyl acetate extract of ***(EAECs)for the first *** addition,the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium(MTS)assay revealed that EAECs and flazin inhibited the transformation of splenic lymphocytes in ***,flazin(20μg·mL^(−1))altered the populations of splenic lymphocyte ***-time quantitative PCR(RT-qPCR)analysis and enzyme-linked immunosorbent assay(EliSA)showed that flazin suppressed the mRNA expression and secretion of TNF-αand IL-2,and reversed Concanavalin A(ConA)-induced mRNA up-regulation and protein secretion of TNF-αand *** blot results showed that flazin reversed ConA-induced increases in p-ERK1/2 and p-p38 in *** conclusion,flazin exhibits effective immunomodulatory function and may be useful for treating immune-related disorders,which indicates the application potential of *** as a functional food or immunomodulator.

关键词： Flazin Crassostrea sikamea Cytokine secretion Immunomodulatory effect Splenic lymphocyte

Mutation Analysis of AVPR2 and AQP2 Gene in Chinese Patients with Congenital Nephrogenic Diabetes Insipidus

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Chemical Research in Chinese Universities 2008年第3期24卷 312-315页

作者： WANG Ying li Hong-jun YU Zhen-xiang bao yong-li WU Yin YU Chun-lei MENG Xiang-ying li Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China Out-patient Department China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China Department of Respiratory Medicine First Hospital of Jilin University Changchun 130021 P. R. China Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Northeast Normal University Changchun 130024 P. R. China

To detect mutations of the aquaporin 2 gene（AQP2） and the arginine vasopressin V2 receptor gene（AVPR2） of Chinese congenital nephrogenic diabetes insipidus, and to establish the foundation for further studying the emergence mechanism of the disease and clinical diagnosis, all the exons and part of introns of AQP2 and AVPR2 genes were amplified with intronic primers, using genomic DNA extracted from three patients with congenital nephrogenic diabetes insipidus and two mothers as template, PCR product was ligated into a T-vector and then sequenced. The result was compared with the database sequence to identify the mutable sites via a BLAST search, the incidence of every mutation was analyzed, and the putative transcription factor binding sites that maybe disturbed were analyzed by MAPPER. Mutation g.1394A〉G in exon 3 of AVPR2 was detected in all the subjects, g.861C〉T（S167L） in exon 2 of AVPR2 and IVS1＋3G〉A in intron of AQP2 were detected, respectively, in two patients, and c.836A〉C in 3′ untranslated region of AQP2 was detected in two patients and one mother. Four mutations were identified. g.1394A〉G of AVPR2 and c.836A〉C of AQP2 have high incidence in patients with nephrogenic diabetes insipidus. Detection on the two sites may become auxiliary diagnosis index of congenital nephrogenic diabetes insipidus.

关键词： Nephrogenic diabetes insipidus Gene mutation AQP2 A VPR2

Preparation of Polyclonal Antibodies Against Testis-specific Protease 50 and Characterization of Antibody Specificity

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Chemical Research in Chinese Universities 2006年第3期22卷 308-311页

作者： ZHANG Jing YANG Ming XU Ze-li bao yong-li WU Yin WANG Yue-zeng MENG Xiang-ying JU Xing-da GU Yang li Yu-xin Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin University Changchun 130031 P. R. China

Testis-specific protease 50 （TSP50） is a testis-specific oncogene, which is abnormally activated in most tested patients with breast cancer. This property makes it an attractive molecular marker and a promising target for the diagnosis and therapy of breast cancer. In order to obtain the protective and specific polyclonal antibodies for further research, TSPS0 cDNA was amplified by RT-PCR from normal human testicular tissue, and inserted into eukaryotic expression vector PeDNA3.1. Rabbit anti-TSPS0 polyclonal antibodies were prepared by means of intramuscular injection of peDNA3.1-TSPS0 into the rabbits. Titem of the anti-sera were measured by EliSA and Western blotting with the E. coli cell lysate containing the induced GST-TSPS0 fusion protein as an antigen. In addition, we examined the expression of TSPS0 in both breast cancer cell line MCF-7 and breast cancer tissue by immunofluorescent and immunohistochemistry analysis.

关键词： Breast cancer EliSA Polyclonal antibody TSPSO