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High-throughput metagenomic analysis of petroleum-contaminated soil microbiome reveals the versatility in xenobiotic aromatics metabolism

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Journal of Environmental Sciences 2017年第6期29卷 25-35页

作者： Yun-juan bao Zixiang Xu Yang Li Zhi Yao Jibin Sun Hui Song National Engineering Laboratory for Industrial Enzymes Chinese Academy of SciencesTianjin 300308China Key Laboratory of Systems Microbial Biotechnology Chinese Academy of SciencesTianjin 300308China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China Key Laboratory of Immune Microenuironment and Disease of the Ministry of Education Tianjin Medical UniversityTianjin 300070China W.M.Keck Center for Transgene Research University of Notre DameNotre DameIN 46556USA

The soil with petroleum contamination is one of the most studied soil ecosystems due to its rich microorganisms for hydrocarbon degradation and broad applications in bioremediation. However, our understanding of the genomic properties and functional traits of the soil microbiome is limited. In this study, we used high-throughput metagenomic sequencing to comprehensively study the microbial community from petroleum-contaminated soils near Tianjin Dagang oilfield in eastern China. The analysis reveals that the soil metagenome is characterized by high level of community diversity and metabolic versatility. The metageome community is predominated by 7-Proteobacteria and a-Proteobacteria, which are key players for petroleum hydrocarbon degradation. The functional study demonstrates over-represented enzyme groups and pathways involved in degradation of a broad set of xenobiotic aromatic compounds, including toluene, xylene, chlorobenzoate, aminobenzoate, DDT, methylnaph- thalene, and bisphenol. A composite metabolic network is proposed for the identified pathways, thus consolidating our identification of the pathways. The overall data demon- strated the great potential of the studied soil microbiome in the xenobiotic aromatics degradation. The results not only establish a rich reservoir for novel enzyme discovery but also provide putative applications in bioremediation.

关键词： Comparative genomicsMetagenomic sequencingTaxonomic analysisOil-contaminated soils

Preparation of chitosan and citric acid crosslinked membrane and its application in quasi-solid supercapacitors

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Rare Metals 2023年第2期42卷 430-437页

作者： Hao-Hang Xu Liang Yue Yang Tang Fan Liu Hong-Jiu Zhu Shu-juan bao Institute for Clean Energy&Advanced Materials School of Materials and EnergySouthwest UniversityChongqing400715China

Quasi-solid electrolytes promote the development of safe and flexible energy storage *** this work,a chitosan and citric acid crosslinked membrane is prepared by a freeze-thaw cross-linking method,in which the chemical crosslinking of chitosan and citric acid increase the viscoelastic behavior of the polymer membrane,and the freeze-thaw assist freeze drying process to create abundant interconnected open-pores and three-dimensional(3D)*** to the good viscoelasticity,excellent electrolyte loading capacity(596%)and high ion conductivity(7.7×10^(-3)S·cm^(-)1),as quasi-solid electrolyte,our proposed chitosan and citric acid crosslinked membrane helps ZnICCFT-ZnSO4lAC hybrid supercapacitor to delivers wide operating voltage,high specific capacity of 100.5 F·g^(-1)and stable cycle life(93%after1000 cycles),which suggests that our proposed freezethaw assisted freeze drying method has great potential in designing quasi-solid state electrolyte for energy storage device.

关键词： Quasi-solid electrolyte Chitosan Freeze-thaw Hybrid supercapacitor

Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura

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Insect Science 2016年第6期23卷 819-828页

作者： bao-juan Zeng Yuan Lv Li-Li Zhang Li-Hua Huang Qi-Li Feng Guangzhou Key Laboratory of Insect Development Regulation and Application Research School of Life Sciences South China Normal University Guangzhou 510631 China

Juvenile hormone （JH） is one of the key insect hormones that regulate metamorphosis. Juvenile hormone diol kinase （JHDK） is an enzyme involved in JH metabolism and catalyzes JH diol to form a polar end product, JH diol phosphate that has no JH activity. In this study, a JHDK complementary DNA （cDNA） was cloned from Spodoptera litura and the structure and expression of the gene was characterized. The cDNA was 714 base pairs in length and encoded a protein of 183 amino acids with a molecular mass of 21 kDa and an isoelectric point of 4.55. Based on the structure, three putative calcium binding motifs and guanosine triphosphate-binding motifs were predicted in the protein. Modeling of the 3-D structure showed that the protein consisted of eight α-helixes linked with loops, with no β-sheets. The gene was expressed in the epidermis, fat body and midgut of fifth and sixth instar larvae. The expression level in the epidermis was lower than in the fat body and midgut. The gene was expressed at higher levels at the early stages than in the later stages of fifth and sixth instar midgut and fat body. The results suggest that this gene may be involved in the regulation of the JH titer in larvae ofS. litura.

关键词： juvenile hormone juvenile hormone diol kinase metabolism Spodoptera litura

ZnO Additive Boosts Charging Speed and Cycling Stability of Electrolytic Zn–Mn Batteries

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Nano-Micro Letters 2024年第4期16卷 293-304页

作者： Jin Wu Yang Tang Haohang Xu Guandie Ma Jinhong Jiang Changpeng Xian Maowen Xu Shu‑juan bao Hao Chen Institute for Clean Energy&Advanced Materials School of Materials and EnergySouthwest UniversityChongqing 400715People’s Republic of China

Electrolytic aqueous zinc-manganese(Zn–Mn) batteries have the advantage of high discharge voltage and high capacity due to two-electron reactions. However, the pitfall of electrolytic Zn–Mn batteries is the sluggish deposition reaction kinetics of manganese oxide during the charge process and short cycle life. We show that, incorporating ZnO electrolyte additive can form a neutral and highly viscous gel-like electrolyte and render a new form of electrolytic Zn–Mn batteries with significantly improved charging capabilities. Specifically, the ZnO gel-like electrolyte activates the zinc sulfate hydroxide hydrate assisted Mn^(2+) deposition reaction and induces phase and structure change of the deposited manganese oxide(Zn_(2)Mn_(3)O_8·H_(2)O nanorods array), resulting in a significant enhancement of the charge capability and discharge efficiency. The charge capacity increases to 2.5 mAh cm^(-2) after 1 h constant-voltage charging at 2.0 V vs. Zn/Zn^(2+), and the capacity can retain for up to 2000 cycles with negligible attenuation. This research lays the foundation for the advancement of electrolytic Zn–Mn batteries with enhanced charging capability.

关键词： Electrolytic aqueous zinc-manganese batteries Electrolyte pH value ZnO electrolyte additive Fast constant-voltage charging ability

Which adjustment methods are suitable for the wind-induced errors of Geonor T-200BM3 precipitation weighing gauges in a periglacial site?

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Advances in Climate Change Research 2023年第5期14卷 707-719页

作者： Lei WANG Ren-Sheng CHEN Wei-Jun SUN Chun-Tan HAN bao-juan HUAI Yan-Ni ZHAO College of Geography and Environment Shandong Normal UniversityJinan 250358China Qilian Alpine Ecology and Hydrology Research Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China

Single Alter shielded T-200BM3 weighing precipitation gauges are widely used in the measurement of all precipitation types(rainfall,snow and mixed precipitation)in unattended boreal or alpine regions,but their original datasets must be adjusted for undercatch errors caused by wind in snowy,windy and harsh ***,previous researchers have developed many adjustment methods for all precipitation types on different time ***,which adjustment method is suitable for T-200BM3 weighing gauge wind-induced error adjustment in harsh alpine regions is ***,precipitation measurement intercomparison experiments were conducted in the Qilian Mountains from July 2018 to July 2021,and eight adjustment methods;were evaluated for wind-induced errors for daily,individual precipitation event,hourly,and half-hourly time scales.Z2004 outperformed the other adjustment methods in regard to the daily measurements of snow and mixed *** individual snowfall events,M2007 reduced the absolute value of RMSE(bias)from 1.44 to 1.32 mm(0.77-0.24 mm)and could be recommended for snowfall event adjustment.K2017-1 attained a better performance than K2017-2 in regard to half-hourly snowfall and mixed sample adjustment and was more suitable for half-hourly snowfall sample adjustment.K2017-1 reduced the absolute value of bias from 0.07 to 0.00 mm for ***,Z2004,M2007,and K2017-1 yielded better adjustment results for the daily accumulation precipitation amount(>2 mm d−1),individual snowfall events(>2 mm per event),and half-hourly accumulation snowfall or mixed samples(>1 mm 30 min−1),***,further intercomparison in different climate regions is needed for trace precipitation samples.

关键词： Evaluation Wind-induced errors Adjustment methods Weighing gauges Periglacial site

Thermochemical Study of Coordination of Holmium Chloride Hydrate with Diethylammonium Diethyldithiocarbamate

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Chemical Research in Chinese Universities 2004年第6期20卷 766-770页

作者： ZHAO Feng-qi CHEN San-ping JIAO bao-juan REN Yi-xia GAO Sheng-li SHI Qi-zhen Department of Chemistry Shaanxi Key Laboratory of Physico-Inorganic ChemistryNorthwest UniversityXi′an 710069P.R.China Xi′an Modern Chemistry Research Institute Xi′an 710065P.R.China

The complex of holmium chloride hydrate with diethylammonium diethyldithiocarbamate(D-DDC) was synthesized via mixing their solutions in absolute alcohol under a dry N 2 atmosphere. The elemental and chemical analyses show that the complex has the general formula Et 2NH 2[Ho(S 2CNEt 2) 4]. It was also characterized by IR spectroscopy. The enthalpies of the dissolution of holmium chloride hydrate and D-DDC in absolute alcohol at 298.15 K, and the enthalpy changes of liquid-phase reactions of the formation of Et 2NH 2[Ho(S 2CNEt 2) 4] at different temperatures were determined by microcalorimetry. On the basis of experimental and calculated results, three thermodynamic parameters(the activation enthalpy, the activation entropy and the activation free energy), the rate constant and three kinetic parameters(the apparent activation energy, the pre-exponential constant and the reaction order) of the liquid-phase reaction of the complex formation were obtained. The enthalpy change of the solid-phase complex formation reaction at 298.15 K was calculated by means of a thermochemical cycle.

关键词： Holmium chloride hydrate Diethylammonium diethyldithiocarbamate(D-DDC) Microcalori-metric method Thermochemistry

Efficient Catalytic Conversion of Polysulfides by Biomimetic Design of “Branch‑Leaf” Electrode for High‑Energy Sodium–Sulfur Batteries

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Nano-Micro Letters 2021年第3期13卷 155-167页

作者： Wenyan Du Kangqi Shen Yuruo Qi Wei Gao Mengli Tao Guangyuan Du Shu‑juan bao Mingyang Chen Yuming Chen Maowen Xu Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationSchool of Materials and EnergySouthwest UniversityChongqing 400715People’s Republic of China Beijing Computational Science Research Center Beijing 100193People’s Republic of China Center for Green Innovation School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083People’s Republic of China College of Environmental Science and Engineering Fujian Normal UniversityFuzhou 350007People’s Republic of China

Rechargeable room temperature sodium–sulfur(RT Na–S)batteries are seriously limited by low sulfur utilization and sluggish electrochemical reaction activity of polysulfide ***,a 3D“branch-leaf”biomimetic design proposed for high performance Na–S batteries,where the leaves constructed from Co nanoparticles on carbon nanofibers(CNF)are fully to expose the active sites of *** CNF network acts as conductive“branches”to ensure adequate electron and electrolyte supply for the Co *** an effective electrocatalytic battery system,the 3D“branch-leaf”conductive network with abundant active sites and voids can effectively trap polysulfides and provide plentiful electron/ions pathways for electrochemical *** calculation reveals that the Co nanoparticles can induce the formation of a unique Co–S–Na molecular layer on the Co surface,which can enable a fast reduction reaction of the ***,the prepared“branch-leaf”CNF-L@Co/S electrode exhibits a high initial specific capacity of 1201 mAh g^−1 at 0.1 C and superior rate performance.

关键词： Co nanoparticles Sodium-sulfur batteries Branch-leaf,biomimetic

Reasonable suppression of polysulfides/polyselenides shuttle based on MXene in Na-SeS_(2)batteries

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Rare Metals 2023年第5期42卷 1594-1602页

作者： Qiu-Ju Yang Jing Zhao Wei Gao Wei Zhong Yu-Ruo Qi Jin Han Shu-juan bao Mao-Wen Xu School of Materials and Energy Southwest UniversityChongqing400715China Helmholtz Institute Ulm Ulm89081Germany Karlsruhe Institute of Technology Karlsruhe76021Germany

Metal-sulfur/selenium batteries have become the focus of new-generation energy storage systems due to the advantages of low-cost and high energy ***,it still suffers from the notorious shuttle of polysulfides/polyselenides,poor electronic conductivity and tremendous volume ***,a dual defense system for polysulfides/polyselenides was proposed and constructed based on *** nitrogen-doped porous carbon(NPC)decorated by Ti_(3)C_(2)T_(x)MXene(M@NPC)was employed as the SeS_(2)host(SeS_(2)@M@NPC).Particularly,Ti_(3)C_(2)T_(x)sheets wrapped on NPC guarantee the rapid ion diffusion and serve as the first barrier for SeS_(2)and dissolved sodium polysulfides/***,the few-layered Ti_(3)C_(2)T_(x)sheets coated on glass fiber separators act as the second barrier for alleviating the shuttle of polysulfides/polyselenides through physical interception and chemical *** this elaborate design,the integrated Na-SeS_(2)battery achieves a high specific capacity of 1243 mAh·g^(-1)at 1.0C,revealing a distinct superiority over its counterparts(SeS_(2)@M@NPC,1083mAh·g^(-1)at 0.5C;and SeS_(2)@NPC,823 mAh·g^(-1)at 0.5C).The findings gained in this work provide a creative idea for the construction of durable room-temperature Na-SeS_(2)batteries based on MXenes and their derivative materials.

关键词： MXene Integrated strategy Shuttle effect Room temperature(RT)Na-SeS_(2)batteries

Expression, subcellular localization and protein-protein interaction of four isoforms of EcR/USP in the common cutworm

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Insect Science 2015年第1期22卷 95-105页

作者： Li-Xia Huang Yan-Jun Gong Jun Gu bao-juan Zeng Li-Hua Huang Qi-Li Feng Laboratory of Molecular and Developmental Entomology Guangdong Provincial Key Laboratory of Biotechnology for Plant DevelopmentSchool of Life Sciences South China Normal University Guangzhou China

Ecdysone receptor （EcR） and ultraspiracle （USP） form heterodimers to mediate ecdysteroid signaling during molting and metamorphosis. Various EcR/USP heterodimers have been reported. However, it is unclear what kind of EcR/USP combination is adopted by lepidopteran insects during the larval-pupal metamorphosis and whether the EcR/USP heterodimer varies among different tissues. To address these questions, two isoforms of each EcR and USP were cloned from the common cutworm, their messenger RNA expression patterns were examined by real-time quantitative polymerase chain reaction in different tissues during the larval-pupal metamorphosis and in the midgut in response to hormonal induction. Furthermore, their subcellular localization and protein-protein interaction were explored by transient expression and far-western blotting, respectively. All the four genes were significantly up-regulated in prepuae and/or pupae. The expression profiles of EcRB1 and USP1 were nearly identical to each other in the epidermis, fat body and midgut, and a similar situation also applied to EcRA and USP2. The three genes responded to 20-hydroxyecdysone （20E） induction except for USP2, and USP1 could be up-regulated by both 20E and juvenile hormone. The four proteins mainly localized in the nucleus and the nuclear localization was promoted by 20E. The protein-protein interaction between each EcR and USP was found in vitro. These results suggest that two types of EcR/USP heterodimer （EeRA/USP2 and EcRB 1/USP1） may exist simultaneously in the common cutworm, and the latter should play more important roles during the larval-pupal metamorphosis. In addition, the types of EcR/USP heterodimer do not vary in the tissues which undergo histolysis and regeneration during metamorphosis.

关键词： ecdysone receptor protein interaction subcellular localization ultraspiracle