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Impact of Phenanthrene on Denitrification Activity and Transcription of Related Functional Genes in Estuarine and Marine Sediments

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Journal of Ocean University of China 2020年第1期19卷 124-134页

作者： SUN Pengfei bai Jie LI Kuiran ZHAO Yangguo TIAN Weijun bai xiaoyan TIAN Yanzhao College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China The Key Laboratory of Marine Environmental Science and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China College of Marine Life Science Ocean University of ChinaQingdao 266003China

The effects of phenanthrene(Phe)on the denitrification activity and denitrifying genes(narG,nirS and nosZ)were evaluated by dose-response experiments in sediments of Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that potential denitrification activity(PDA),N2O,NO3−and NO2−reduction rates of both areas were inhibited with an increase of Phe *** PDA,N2O,NO3−and NO2−reduction rates of both areas was highest and lowest in the control(DRE:0.453,0.427,7.439 and 3.222mgNkg−1 h−1,JZB:0.592,0.555,8.470 and 3.793mgNkg−1 h−1)and highest Phe amended treatments(DRE:0.069,0.001,4.486,and 1.563 mgNkg−1 h−1;JZB:0.114,0.024,5.527 and 2.200 mgNkg−1 h−1).The inhibition rate of PDA was highest,follow by NO2−reduction and then NO3−***,with the increasing of Phe concentrations,total bacteria count and the abundance of denitrifying genes were *** N2O accumulation was promoted with the addition of Phe for both *** on the comparison of EC50 values,denitrifiers harboring three genes were more sensitive to Phe than PDA,and denitrifiers harboring nirS gene were more sensitive,followed by nosZ gene,and then narG ***,according to correlation analysis,the relative abundance of denitrifying genes was much more positively correlated with PDA,NO3−and NO2−reduction than total bacteria *** addition,the denitrification activity and total bacteria count in JZB were more inhibited than that of *** study is useful for understanding the impact of Phe pollution on denitrification in estuary and marine sediments,with profound implications for the management of aquatic ecosystems regarding eutrophication(N-removal)and greenhouse effect.

关键词： phenanthrene effect denitrification denitrifying genes estuarine sediment marine sediment

Anomalous changes of temperature and ozone QBOs in 2015−2017 from radiosonde observation and MERRA-2 reanalysis

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Earth and Planetary Physics 2021年第3期5卷 280-289页

作者： xiaoyan bai KaiMing Huang ShaoDong Zhang ChunMing Huang Yun Gong School of Electronic Information Wuhan UniversityWuhan 430072China Key Laboratory of Geospace Environment and Geodesy Ministry of EducationWuhan 430072China State Observatory for Atmospheric Remote Sensing Wuhan 430072China

Anomalous changes of zonal wind quasi-biennial oscillation(QBO)in winter 2015−2016 have received close *** radiosonde and satellite observations and reanalysis data,we investigate anomalous changes in temperature and ozone QBOs from the lower to middle *** wind shear direction is reversed due to unexpected changes of zonal wind QBO at about 24−30 km,the shortest cold phase at 21−27 km appears in temperature *** is different from the completely interrupted westward phase in zonal wind QBO,while the longest cold phase above almost 27 km lasts for 2−3 years from 2015 to 2017,owing to the absence of corresponding warm *** scale reduction of temperature QBO causes a small temperature anomaly,thus the thermal wind relationship looks seemingly different from that in the other regular QBO *** in the ozone mixing ratio anomaly shows a double-peak with inverse phase,and its phase below(above)30 km is in agreement with(opposite to)the phase of temperature QBO because of different control mechanisms of *** temperature QBO variation,QBO in the ozone mixing ratio anomaly exhibits a less positive phase at 20−30 km in 2016−2017,and a very long positive phase above 30 km from 2015 to *** in total column ozone shows a small peak in winter 2016−2017 since ozone is mainly concentrated at 20 to 30 *** changes of temperature and ozone QBOs due to unexpected QBO zonal wind variation can be well-explained according to thermal wind balance and thermodynamic balance.

关键词： QBO temperature anomaly ozone anomaly thermal wind balance

Investigations on the time evolution of the plasma density in argon electron-beam plasma at intermediate pressure

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Plasma Science and Technology 2017年第3期19卷 47-53页

作者： xiaoyan bai Chen CHEN Hong LI Wandong LIU KTX Laboratory and Department of Modem Physics University of Science and Technology of China Hefei 230026 People＇s Republic of China State Key Laboratory of Intense Pulsed Radiation Simulation and Effect Northwest Institute of Nuclear Technology Xi＇an 710024 People＇s Republic of China

The time evolution of the argon electron-beam plasma at intermediate pressure and low electron beam intensily was *** applying the amplitude modulation with the frequency of 20 Hz on the stable beam current,the plasma evolution was studied.A Faraday cup was used for the measurement of the electron beam current and a single electrostatic probe was used for the measurement of the ion *** results indicated that the ion current was in phase with the electron beam current in the pressure range from 200 Pa to 3000 Pa and in the beam current range lower than 20 mA,the residual density increased approximately linearly with the maximum density in the log-log plot and the fitting coefficient was irrelative to the *** then three kinds of kinetic models were developed and the simulated results given by the kinetic model,without the consideration of the excited atoms,mostly approached to the experimental *** indicated that the effect of the excited atoms on the plasma density can be ignored at intermediate pressure and low electron beam current intensity,which can greatly simplify the kinetic *** the *** decrease of the plasma density when the beam current was suddenly off was studied based on the simplified model and it was found that the decease characteristic at intermediate pressure was approximate to the one at high pressure at low electron beam intensity,which was in good accordance with the experimental results.

关键词： electron-beam plasma kinetic model time evolution residual density phase lag

Reliability of a Tissue Microarray in Detecting Thyroid Transcription Factor-1 Protein in Lung Carcinomas

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Chinese Journal of Clinical Oncology 2007年第1期4卷 9-15页

作者： xiaoyan bai Hong Shen Department of Pathology Southern Medical University GuangZhou 510515 China

OBJECTIVE To compare the expression of the thyroid transcription factor-1 （TTF-1） in human normal adult type Ⅱ alveolar epithelial cells, embryonic pneumocytes and cancer cells of lung carcinoma and metastatic lymph nodes using a tissue microarray （TMA） along with paired conventional full sections, and to investigate the reliability of tissue microarrays in detecting protein expression in lung carcinoma. METHODS A lung carcinoma TMA including 765 cores was constructed. TTF-1 protein expression in both TMA and paired conventional full sections were detected by the immunohistochemical SP method using a monoclonal antibody to TTF-1. A PU （Positive Unit） of TTF-1 protein was assessed quantitatively by the Leica Q500MC image analysis system with results from the paired conventional full sections as controls. RESULTS There was no significance between TMA and paired conventional full sections in TTF-1 expression in different nuclei of the lung tissue. CONCLUSION TTF-1 protein expression in lung carcinoma detected by TMA was highly concordant with that of paired full sections. TMA is a reliable method in detecting protein expression.

关键词： lung carcinoma transcription factor-1(TTF-1) liability. tissue microarray thyroid immunohistochemistry reliability.

Study of latch-up immunization in bulk CMOS integrated circuits exposed to transient ionizing radiation

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Science China(Technological Sciences) 2012年第11期55卷 3242-3247页

作者： LI RuiBin CHEN Wei LIN DongSheng YANG ShanChao bai xiaoyan WANG GuiZhen LIU Yan QI Chao MA Qiang Northwest Institute of Nuclear Technology Xi'an 710024China

This paper presents experimental results of transient gamma irradiation effects on two kinds of *** is a two-stage circuit consisting of a bipolar power device L7805CV and a bulk complementary metal-oxide-semiconductor(CMOS) device IDT6116,the other is a two-stage circuit consisting of a bipolar power device L7805CV and the equivalent circuit of the parasitic P-N-P-N structure in bulk CMOS *** results show that the L7805CV's output interruption after transient irradiation can prevent latch-up from occurring on the second stage *** demanded minimum interruption duration to avoid latch-up varies with dose rate,and this is confirmed by the experimental results.

关键词： transient radiation' latch up dose rate bulk CMOS device

Impact of TID on response to pulsed X-ray irradiation in the bipolar operational amplifier

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Science China(Technological Sciences) 2015年第2期58卷 390-396页

作者： LI Rui Bin WANG Gui Zhen CHEN Wei MA Qiang LIU Yan LIN Dong Sheng YANG ShanChao bai xiaoyan QI Chao JIN XiaoMing State Key Laboratory of Intense Pulsed Radiation Simulation and Effect Northwest Institute of Nuclear Technology

Groups of a typical operational amplifier-μA741 were irradiated in a cobalt unit, each group accumulating a different total ionizing dose （TID）. The results showed that the TID caused power consumption current and slew rate （SR） to degenerate in ultra-linearity, owing to a severe reduction in the current gain of the internal LPNP transistors. Pulsed X-ray irradiation experiments were carried out on the μA741 groups with different values, and the results revealed that the impact on the response to the pulsed X-ray irradiation was greater when the devices absorbed more TID. The mechanism for this is explained on the basis of the circuit construction of the μA741; the sensitive parameters of the circuit were obtained via simulation on SP1CE. The simulation results additionally showed that if the sensitive parameters were optimized, the duration of interruption caused by the pulsed X-ray irradiation would be reduced significantly. In addition, several proposals are provided for hardening the devices.

关键词： TID pulsed X-ray irradiation operational amplifier simulation

Generation of functionally mature neurons from a telomerase-immortalized human glial progenitor cell line

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Neural Regeneration Research 2009年第2期4卷 106-110页

作者： Yun bai xiaoyan Zhang Aili Lu Juniun Xiao Li Shen Department of Cell Biology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China

BACKGROUND： It has long been thought that neurons and glial cells are produced from distinct progenitor pools, but recent studies suggest that the glial progenitor cell in the subventricular zone can generate neurons in the adult rodent brain. OBJECTIVE： To investigate the ability of a telomerase-immortalized human glial progenitor cell line to differentiate into functionally mature neurons. DESIGN, TIME AND SETTING： The cellular and molecular biology experiment was performed at the Cell Biology Laboratory in the School of Basic Medical Sciences, Peking University Health Science Center, between July 2007 and May 2008. MATERIALS： A telomerase reverse transcriptase immortalized human glial progenitor cell line, was established in our laboratory. Dibutyryl cyclic AMP was purchased from Sigma （USA）. Specific antibodies against glial fibrillary acidic protein, ~ -tubulin-Ⅲand A2B5 were purchased from Chemicon, USA. Polyclonal antibodies against nestin and MAP2ab were obtained from Neomarker, USA. METHODS： The telomerase immortalized human glial progenitor cell line was passaged and maintained in growth medium consisting of DMEM/F12 （1：1） with N2 supplement （1%, v/v）, L-Glutamine （2 mmol/L）, epidermal growth factor （20 ng/mL）, basic fibroblast growth factor （20 ng/mL） and 3% fetal bovine serum. Neuronal differentiation was induced by the addition of 1 mmol/L dibutyryl cyclic AMP and 10% fetal bovine serum. MAIN OUTCOME MEASURES： Neuronal differentiation was evaluated by RT-PCR, quantitative PCR, immunofluorescence staining, Western blot analysis and electrophysiology. RESULTS： After dibutyryl cyclic AMP induction in the telomerase immortalized human glial progenitor cells, the expression of neuronal-specific marker mRNAs and proteins increased significantly. Concurrently, an apparent fast inward Na^＋ current was evoked in the cells after induction. CONCLUSION： This study suggests that some human glial progenitor ceils are indeed capable of generating functionall

关键词： telomerase immortalization human glial progenitor cells neuronal differentiation

Bibliometrics and visual analysis for clinical research on intervention of traditional Chinese medicine nursing technology on rheumatoid arthritis

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Journal of Integrative Nursing 2022年第1期4卷 20-25页

作者： Ling TANG Jianni QU Hong GUO Mei WANG Nini XU xiaoyan bai Xueyan FAN Zhixuan XU Yihui TIAN Fen ZHOU Department of Nursing Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina School of Nursing Beijing University of Chinese MedicineBeijingChina School of Nursing and Rehabilitation Shandong UniversityShandongChina#contributed equally to this work.

Objective:The objective of this study is to explore the current situation,clinical research hot spots,and trends of traditional Chinese medicine(TCM)nursing technology in rheumatoid arthritis(RA),to draw a panorama of clinical research,to provide the basis and clues for subsequent high-level evidence integration and further in-depth *** and Methods:Seven databases including China National Knowledge Infrastructure,Chinese Scientific Journal Database(VIP),Wanfang Database,Chinese Biomedical Literature Database(Sino-Med),PubMed,Embase,and the Cochrane Library were *** bibliometric method and visualization software CiteSpace were used to conduct a multi-dimensional analysis of the included ***:A total of 805 pieces of literature were included(of them,one was written in German and four in English),and the number of published literature showed an increasing trend year by *** were only 30(3.73%)pieces of literature published in nursing *** hot spots of the co-occurrence map were concentrated in:(1)the nursing of fumigation in RA;(2)the efficacy evaluation of TCM nursing technology on pain,joint malformation,and joint dysfunction caused by *** included literature themes focused on five TCM technologies:fumigation,moxibustion,acupoint patching,acupoint injection,and Chinese herbal *** beginning year and strength in TCM fumigation were 2012 and 5.2,and those in moxibustion were 2011 and ***:The related studies are on the *** has entered the field of international readers with clear research hot ***,there are still shortcomings such as little literature published in Chinese nursing core journals,few non-Chinese-related documents,and a lack of international exchanges and *** current research hot spots in this field are TCM fumigation,and the cutting-edge trend of future research may be fumigation and moxibustion *** is suggested that further research can focus on ev

关键词： Literature metrology rheumatoid arthritis traditional Chinese medicine nursing technology visual analysis Wangbi

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人民币汇率制度改革历程及逻辑

世界经济研究 2008年第12期 29-34页

作者：白晓燕武汉大学经济与管理学院

本文从回顾人民币汇率制度改革的历程入手,多角度论证推动人民币汇率制度改革的内在逻辑。研究认为,人民币汇率制度改革以国内经济体制改革进程为内在约束,注重汇率的实用性、改革的渐进性、与其他经济体制改革顺序安排的合理性,并且逐... 详细信息

本文从回顾人民币汇率制度改革的历程入手,多角度论证推动人民币汇率制度改革的内在逻辑。研究认为,人民币汇率制度改革以国内经济体制改革进程为内在约束,注重汇率的实用性、改革的渐进性、与其他经济体制改革顺序安排的合理性,并且逐渐遵守国际规范和兼顾国际反应,走出了一条以市场为导向、独具特色的汇率制度改革道路。从近期和中期来看,深化人民币汇率制度改革的重点是完善汇率形成机制和加强外汇市场建设。

关键词：人民币汇率制度改革汇率形成机制