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Reduced Expression and Genetic Alterations of ING4 in Human Stomach Adencarcinoma

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Reduced Expression and Genetic Alterations of ING4 in Human ...

中国遗传学会“发育、遗传和疾病”研讨会

作者： Ming Li~1 Yan Jin~1 Changlong Guo~1 jing bai~1 feng Chen~1 Songbin Fu~(1,2) 1.Laboratory of Medical Genetics,Harbin Medical University,Harbin,150081,China 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin,150081,China

ING4 is a member oflNG gene family and harbors the PHD domain and NLS ***4 is reported to interact with tumor suppressor p53 and found to be involved in various biological activities, including cell cycle arrest,regulation of gene transcription,DNA repair and apoptosis,ING41 is located in nucleus whereas other three variants are located in *** clarify the role of ING4 in human stomach adencarcinoma,we first compared ING4 expression in four human gastric adenocarcinoma cell lines by RT-PCR and Real-time ***/NG4 mRNA level was found in all cell lines,while there is lower ING4 expression found in differentiated cell ***,fourty samples of human stomach adenocarcinoma tissues and matched no-tumor tissues were investigated using RT-PCR and Real-time PCR. ING4 expression level is significantly decreased in stomach adenocarcinoma tissues（P＜0.05）and markedly related to gender and infiltration（P＜0.05）.Then,Expression of ING4 is analyzed among other fourty human stomach adenocarcinoma samples without matched normal tissues and fourty human stomach adenocarcinoma with matched normal tissues,through tissue microarray analysis ING4 expression was found to locate in the nucleus and cytoplasm,especially and interestingly in the parietal ceil,while expression of ING4 is stronger than cytoplasmic *** expression of ING4 was reduced greatly in stomach adenocarcinoma tissues compared with normal stomach tissues（P＜0.01）,but it has no significant relationship with the clinicopathologicai parameters in patients of stomach *** last,we checked mutations in four human gastric adenocarcinoma cell lines and nine random tumor samples using clone sequence,when we found complete and correct ORF sequences of/NG41,/NG42 and ING44, and various mutations in splice variants ING41 and/NG42 as *** these findings suggest that the inhabitation of ING4 gene expression and mutations of ING4 may be involved in the initiation and proceeding of stomach a

关键词： Stomach adenocarcinoma Anti-oncogene ING4

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浅谈CAI在大学体育专业理论课中的应用

体育科技文献通报 2008年第5期16卷 127-128页

作者：韩玉彬白敬锋田蕾蕾北京体育大学研究生部北京100084 中国传媒大学体育部北京100024 景德镇陶瓷学院体育系江西景德镇333001

随着现代教育技术手段的不断进步,CAI进入大学体育专业理论课堂是一种发展的必然。文章论述了体育专业理论课中CAI课件的主要形式,阐明了CAI课件应用的优点,指出了CAI课件应用中要注意的问题。

关键词：大学体育课程

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肿瘤热疗的区域多点温控研究

中国医学物理学杂志 2008年第4期25卷 712-715页

作者：陈树湛白景峰陈亚珠上海交通大学生物医学仪器研究所上海200030

目的:温度控制是肿瘤热疗过程中至关重要的环节,本文结合多点测温系统和射频肿瘤消融系统,以提高肿瘤热疗的疗效及安全性为出发点,探讨肿瘤热疗的单点温控以及区域多点温控的方法和策略。方法:分析并综合模糊控制和PID控制算法的优缺点... 详细信息

目的:温度控制是肿瘤热疗过程中至关重要的环节,本文结合多点测温系统和射频肿瘤消融系统,以提高肿瘤热疗的疗效及安全性为出发点,探讨肿瘤热疗的单点温控以及区域多点温控的方法和策略。方法:分析并综合模糊控制和PID控制算法的优缺点,提出了性能更好的模糊-改进PID温控方法,用于对热疗进行单点温控,再利用区域多点温控策略实现区域多点温控。结果:离体猪肝实验表明,模糊控制的动态特性较好,而稳态特性较差;改进PID控制的稳态特性较好,而动态特性较差;模糊—改进PID控制方法综合了模糊控制和改进PID控制的优点,增强了温度控制的动态性能和稳态性能;区域多点温控策略能很好地实现区域实时温度控制。结论:本文提出的模糊—改进PID控制方法和区域多点温控策略能进一步改善热疗系统的性能。

关键词：热疗模糊—PID控制多点测温区域温控

单针水冷式射频消融过程中组织热损伤区域的有限元分析

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中国医学物理学杂志 2007年第3期24卷 197-199页

作者：范晓筠白景峰陈亚珠上海交通大学生物医学仪器研究所上海200030

目的:提出单针水冷式射频消融系统在治疗过程中对生物组织热损伤的仿真分析。方法:建立电-热场耦合有限元模型,描述射频消融的电场强度、特征吸收率以及水冷式射频针周围组织的温度分布。考虑了组织的电导不变及电导随温度变化的两种情... 详细信息

目的:提出单针水冷式射频消融系统在治疗过程中对生物组织热损伤的仿真分析。方法:建立电-热场耦合有限元模型,描述射频消融的电场强度、特征吸收率以及水冷式射频针周围组织的温度分布。考虑了组织的电导不变及电导随温度变化的两种情况,比较两者的最大电场强度与热损伤区域(超过50℃边界)。结果:在相同的治疗电压和治疗时间下,电导变化模型比电导不变模型的射频最大电场强度增高7.9%,热损伤范围增大19.6%。结论:本文对射频消融术的治疗参数选择有着较好的临床参考价值。

关键词：水冷式射频有限元热损伤区域

New exploration on pathogenesis and early diagnosis of gestational diabetes

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World Journal of Clinical Cases 2025年第1期13卷 1-5页

作者： Hua bai Department of Neurology The Third Affiliated Hospital of Guizhou Medical UniversityDuyun 558099Guizhou ProvinceChina

Gestational diabetes mellitus(GDM)refers to varying degrees of abnormal glucose metabolism that occur during pregnancy and excludes patients pre-viously diagnosed with *** is a unique among the four subtypes of diabetes classified by the international World Health Organization *** GDM patients constitute a small proportion of the total number of diabetes cases,the incidence of GDM has risen significantly over the past decade,posing substantial risk to pregnant women and ***,it warrants considerable *** pathogenesis of GDM is generally considered to resemble that of type II diabetes,though it may have distinct *** blood biochemical proteins in the context of GDM can help elucidate its pathogenesis,thereby facilitating more effective prevention and management *** article reviews this critical clinical issue to enhance the medical community's sufficient understanding of GDM.

关键词： Gestational diabetes mellitus Pathogenesis Proteomics RBP4 ANGPTL8

Effect of cooling temperature on the microstructure and corrosion behavior of X80 pipeline steel

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International Journal of Minerals,Metallurgy and Materials 2020年第3期27卷 347-353页

作者： jing Ma Fan feng bai-qing Yu Hai-feng Chen Li-feng Fan School of Materials Hebei University of Science and TechnologyShijiazhuang 050018China Hebei Key Laboratory of Material Near-Net Forming Technology Shijiazhuang 050018China School of Transportation Inner Mongolia UniversityHohhot 010070China

Dual-phase accelerated cooling(DPAC) was applied to X80 pipeline steel to obtain its microstructure with different amounts of bainite and ferrite. The microstructure, hardness, and polarization behaviors of the steel, cooled to different temperatures, were investigated. Results showed that, with decreasing cooling temperature, the amount of polygon ferrite(PF) increased while that of acicular ferrite(AF) decreased. The amount of bainite correspondingly decreased, except when cooled to 760°C. Moreover, the grain size of ferrite increased. The corrosion behaviors of different phases were distinct. Martensite/austenite(M/A) islands presented at the grain boundary of the PF phase caused small pits. Numerous micro-corrosion cells were formed in the AF and bainite phases, where micropores were prone to form. X80 pipeline steel cooled to 700°C had the best corrosion resistance in the simulated seawater. The decreased amount of the PF phase reduced the area of cathode, resulting in slight corrosion. About 40 vol% of the bainite phase provided strength while the PF phase provided adequate ductility to the X80 steel. It was concluded that the appropriate cooling temperature was 700°C for ideal corrosion resistance and mechanical properties.

关键词： X80 pipeline steel Dual-phase accelerated cooling microstructure corrosion behavior

Inhibitory role of focal adhesion kinase on anoikis in lung cancer cell,A549

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Inhibitory role of focal adhesion kinase on anoikis in lung ...

遗传学进步与人口健康高峰论坛

作者： Gaiyun Liu~1 Xiangning Meng~1 Jiaxi Wang~1 Yan Jin~1 jing bai~1 feng Chen~1 Songbin Fu~(1,2) (1.Laboratory of Medical Genetics,Harbin Medical University,Harbin,China 150081 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin,China 150081)

关键词：

FN promotes migration and invasion of A549 lung cancer cells through the activation of focal adhesion kinase

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FN promotes migration and invasion of A549 lung cancer cells...

遗传学进步与人口健康高峰论坛

作者： Xiangning Meng~1 Gaiyun Liu~1 Jiaxi Wang~1 Yan Jin~1 jing bai~1 feng Chen~1 Songbin Fu~(1,2) (1.Laboratory of Medical Genetics,Harbin Medical University,Harbin,China 150081 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin,China 150081)

Higher levels of focal adhesion kinase(FAK)are expressed in lung metastatic ***,the signaling pathways and their mechanisms that control lung cancer cell migration and invasion,important componets of cancer metastasis,are not well *** sought to identify the mechanism of integrin-mediated phosphorylation of FAK and its downstream signals as well as the role of FAK in lung cancer *** results demonstrated that phosphorylated FAK(Tyr397,Tyr861 and Tyr925)and its downstream signals such as,Src,P13K,AKT,ERK,and JNK were detected in lung cancer cells A549 attached to

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Inhibitory Role of Focal Adhesion Kinase on Anoikis in Lung Cancer Cell,A549

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Inhibitory Role of Focal Adhesion Kinase on Anoikis in Lung ...

中国遗传学会“发育、遗传和疾病”研讨会

作者： Gaiyun Liu~1 Xiangning Meng~1 Jiaxi Wang~1 Yah Jin~1 jing bai~1 feng Chen~1 Songbin Fu~(1,2) 1.Laboratory of Medical Genetics,Harbin Medical University,Harbin,150081,China 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin,150081,China

Resistance to anoikis is a characteristic of malignant cells with increased tumorigenesis and *** FAK activity has been strongly implicated in the development,growth,progression,and metastasis of human *** this report,we studied the functional role of FAK as well as its downstream mediators in human lung cancer cell line,***,in cells overexpressing siRNA-FAK, more apoptotic cells and downregulation ofp-ERK、p-Pl3K、p-SRC、p-P38 were ***, in detached A549 cells,enhanced FAK phosphorylations(Tyr397,Tyr861,Tyr925)were detected in a time-dependent *** provided evidence that activities of the downstream signaling(p-ERK,p-JNK, p-P38,p-PI3K/AKT,p-SRC)were changed in the detached A549 ***,specific inhibitors of MEKI、PI3K and SRC,PD98059、LY294002 and PP2 were used to confirm the importance of ERK、PI3K and SRC in modulating cancer cell ***,our data suggested SRC might be one of the key protein tyrosine kinases responsible for the detachment-induced FAK activation in the lung tumor *** 3K/AKT,MAPK-ERK,and perhaps MAPK-P38,but not MAPK-JNK appear to be the key downstream effecters of FAK in mediating the cell survival *** increased FAK activity upon cell detachment may contribute to the metastasis potential of malignant tumors.

关键词： Lung cance Anoikis Focal adhesion kinase