检索结果-南通市图书馆

香草扶正合剂对Lewis肺癌小鼠肿瘤组织能量代谢及PD-L2、RGMb表达的影响

在线全文

学校读者我要写书评

暂无评论

中国中医药科技 2025年第1期32卷 20-25页

作者：程权傅华洲杭州市第一人民医院浙江杭州310006

目的:探讨香草扶正合剂(Xiangcao fuzheng Mixture,XCFZ)对Lewis肺癌小鼠的抑瘤效应及机制。方法:采用Lewis肺癌细胞株培养移植法建立肺癌荷瘤小鼠模型,随机分为模型组,顺铂(DDP)组,XCFZ低、中、高剂量组,联合组,每组10只;造模第2天开始... 详细信息

目的:探讨香草扶正合剂(Xiangcao fuzheng Mixture,XCFZ)对Lewis肺癌小鼠的抑瘤效应及机制。方法:采用Lewis肺癌细胞株培养移植法建立肺癌荷瘤小鼠模型,随机分为模型组,顺铂(DDP)组,XCFZ低、中、高剂量组,联合组,每组10只;造模第2天开始XCFZ低、中、高剂量组分别予9.3、18.6、37.2 g/kg XCFZ灌胃;顺铂组以5 mg/kg顺铂溶液隔天腹腔注射,同时以等量双蒸水灌胃,联合组以XCFZ中剂量灌胃和顺铂腹腔注射联合给药,模型组按照等量双蒸水灌胃,每天1次,连续给药14 d;称取小鼠瘤质量及去瘤后体质量并计算抑瘤率,Real-time PCR法检测肺癌组织三磷酸腺苷合酶β亚基(ATP 5B)mRNA表达及细胞色素C氧化酶亚单IV(COX IV)mRNA表达水平;高效液相色谱法(HPLC)检测一磷酸腺苷(AMP)、二磷酸腺苷(ADP)、三磷酸腺苷(ATP)含量。TUNEL染色观察肿瘤细胞凋亡。Western blot检测肿瘤组织细胞程序性死亡配体-2(PD-L2)和反义导向分子(RGMb)表达水平。结果:与模型组比较,联合组抑瘤率最高,其次为顺铂组,XCFZ中剂量组。与模型组比较,XCFZ中剂量组去瘤后体质量升高(P<0.05),顺铂组降低(P<0.05)。与模型组比较,顺铂组及XCFZ高、中剂量组ATP 5B mRNA,COX IV mRNA表达显著降低(P<0.05)。与模型组比较,各给药组ATP含量均降低(P<0.05或P<0.01);与顺铂组比较,联合组ATP含量显著降低(P<0.05或P<0.01);与模型组比较,联合组及XCFZ各剂量组ADP/ATP及AMP/ATP显著升高(P<0.05或P<0.01);与顺铂组比较,XCFZ中剂量组ADP/ATP及AMP/ATP显著升高(P<0.05或P<0.01)。TUNEL染色可见模型组肿瘤细胞凋亡稀少,而各给药组肿瘤细胞凋亡明显增多;各给药组肿瘤细胞稀疏,细胞间空洞较多;DDP组和联合组可见大量坏死细胞,肿瘤细胞抑制萎缩,伴大量碎裂和固缩细胞核及多见胞间空洞。与模型组比较,各用药组PD-L2和RGMb蛋白表达水平显著降低(P<0.05);与顺铂组比较,联合组及XCFZ中、高剂量组PD-L2/RGMb蛋白表达显著降低(P<0.05或P<0.01)。结论:XCFZ对Lewis肺癌小鼠具有抑瘤作用,且可提高小鼠的体质量,其机制可能与抑制肿瘤组织能量代谢和下调PD-L2和RGMb蛋白表达相关。

关键词：香草扶正合剂肺癌 ATP 5B COX IV PD-L2/RGMb 小鼠

在线全文

学校读者我要写书评

暂无评论

基于自调整模糊分析的煤矿通风量调节方法

自动化技术与应用 2025年第2期44卷 167-171页

作者：白水全王磊国电建投内蒙古能源有限公司察哈素煤矿内蒙古鄂尔多斯017209 煤炭科学技术研究院有限公司矿山智能通风事业部北京100028

在煤矿通风量的调节工作中,面对复杂的工况,调节方法适应性比较差,存在一定的调节延迟和风量误差。提出基于自调整模糊分析的煤矿通风量调节方法。计算风机的各项工作参数,确定风量初值,利用自调整模糊分析技术建立模糊控制器,将风机各... 详细信息

在煤矿通风量的调节工作中,面对复杂的工况,调节方法适应性比较差,存在一定的调节延迟和风量误差。提出基于自调整模糊分析的煤矿通风量调节方法。计算风机的各项工作参数,确定风量初值,利用自调整模糊分析技术建立模糊控制器,将风机各项参数作为输入量,经过模糊处理、模糊决策等流程,计算需风量,同时建立煤矿通风系统能耗方程,以能耗最低为目的,结合需风量计算结果,调节煤矿通风量。实验结果表明:面对不同的工况,提出的调节方法在风量调节上相对误差小,调节延迟低速度快,在实际应用上具有良好的适应性。

关键词：自调整模糊分析煤矿通风网络通风量调节

GREENCOMMUTE： AN INFLUENCE-AWARE PERSUASIVE RECOMMENDATION APPROACH FOR PUBLIC-FRIENDLY COMMUTE OPTIONS

在线全文

学校读者我要写书评

暂无评论

Journal of Systems Science and Systems Engineering 2018年第2期27卷 250-264页

作者： Shiqing Wu quan bai Sotsay Sengvong School of Engineering Computer and Mathematical Sciences Auckland University of Technology New Zealand

Negative impacts produced by transportation sector have increased in parallel with the increase of urban mobility. In this paper, we introduce GreenCommute, a novel recommendation system which can facilitate commuters to take public fi＇iendly commute options, while provide support to alleviate the external cost in society, such as traffic pollution, congestion and accidents. In the meanwhile, a rewarding mechanism for persuading commuters is embedded in the proposed approach for balancing the conflict between personal needs and social aims. The allocation of reward values also takes users＇ influential degrees in the social network into consideration. Experimental results show that the GreenCommute can promote public friendly commute options more effectively in comparison to the traditional recommendation system.

关键词： Recommendation system agent-based modelling social influence reward public transport

EDITORIAL SPECIAL ISSUE ON AGENT-BASED MODELLING FOR COMPLEX SYSTEMS

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Systems Science and Systems Engineering 2018年第2期27卷 130-133页

作者： quan bai Fenghui Ren School of Engineering Computer & Mathematical Sciences Auckland University of Technology Auckland New Zealand School of Computing and lnformation Technology University of Wollongong Wollongong Australia

Computer based modelling and simulation has become useful tools to facilitate humans to understand systems in different domains, such as physics, astrophysics, chemistry, biology, economics, engineering and social science. A complex system is featured with a large number of interacting components （agents, processes, etc.）, whose aggregate activities are nonlinear and self-organized. Complex systems are hard to be simulated or modelled by using traditional computational approaches due to the complex relationships of components and distributed features of resources, and dynamic work environments. Meanwhile, smart systems such as multi-agent systems have demonstrated advantages and great potentials in modelling and simulating complex systems.

关键词：

[Os(N^N)(CO)_2I_2]配合物的密度泛函理论研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

吉林化工学院学报 2013年第9期30卷 4-8页

作者：金丽张建坡李岩郝希云孙秀云白福全吉林化工学院化学与制药工程学院吉林吉林132022 吉林化工学院科研处吉林吉林132022 吉林大学理论化学计算国家重点实验室吉林长春130023

采用DFT和TD-DFT方法对一系列锇配合物[Os(N^N)(CO)2I2]的基态和激发态几何结构、电子结构、吸收光谱和发射光谱进行了系统的理论研究.计算结果显示出,变换配合物1-3的N^N配体,只是些微的影响了基态和激发态的几何结构,但是导致了较大... 详细信息

采用DFT和TD-DFT方法对一系列锇配合物[Os(N^N)(CO)2I2]的基态和激发态几何结构、电子结构、吸收光谱和发射光谱进行了系统的理论研究.计算结果显示出,变换配合物1-3的N^N配体,只是些微的影响了基态和激发态的几何结构,但是导致了较大的电子结构的差异.配合物1-3的最高占据分子轨道(HOMO)主要由Os原子和CO配体共同占据,而三个配合物的最低空轨道(LUMO)都占据在N^N配体上.因此三个配合物的最低能吸收(488(1),474(2)和559 nm(3))被指认为MLCT/LLCT混合电荷转移跃迁.并且,1-3的最低能发射分别位于585、557和611 nm,具有3[π*(N^N)→d(Os)]和3ππ*(3MLCT/3LLCT)的混合跃迁特征,这和最低能吸收的跃迁特性相一致.

关键词：锇(Ⅱ)配合物电子结构光谱特征 TD-DFT

噬菌体裂解酶TSPphg对金黄色葡萄球菌生物被膜的抑制作用及该过程转录组变化

在线全文

学校读者我要写书评

暂无评论

微生物学杂志 2025年第1期45卷 24-33页

作者：李鑫慈百全王佳佳王峰昆明理工大学生命科学与技术学院云南昆明650500 云南省高校饲用抗生素替代技术工程研究中心云南昆明650500

研究噬菌体裂解酶对细菌生物被膜(Bacterial biofilm,BBF)的作用效果及转录机制。细菌生物被膜的形成是细菌耐药性产生的重要原因之一。金黄色葡萄球菌(Staphylococcus aureus)是常见的易形成生物被膜的致病微生物,生物被膜的形成给临... 详细信息

研究噬菌体裂解酶对细菌生物被膜(Bacterial biofilm,BBF)的作用效果及转录机制。细菌生物被膜的形成是细菌耐药性产生的重要原因之一。金黄色葡萄球菌(Staphylococcus aureus)是常见的易形成生物被膜的致病微生物,生物被膜的形成给临床细菌性感染的治疗和医疗设备表面细菌的清除带来很大的困难。使用结晶紫染色法、XTT(2,3-BIS(2-methoxy-4-nitro-5-sulfonyl)-2H-tetrazolium-5-carboxanilide)实验、荧光显微镜(Fluorescence Microscope,FM)及扫描电子显微镜(Scanning Electron Microscope,SEM)观察等方法研究噬菌体裂解酶TSPphg对金黄色葡萄球菌生物被膜的抑制效果,利用转录组测序得到噬菌体裂解酶TSPphg降解和抑制金黄色葡萄球菌生物被膜过程中的差异基因,并采用加权共表达网络分析(Weighted Correlation Network Analysis,WGCNA)获取其关键模块,进行GO功能注释,并构建其调控因子网络。纯化获得噬菌体裂解酶TSPphg重组蛋白,实验中发现噬菌体裂解酶TSPphg对金黄色葡萄球菌生物被膜形成具有良好的抑制作用,且显微镜下观察到作用后的生物被膜密度显著变小,说明其能达到清除细菌生物被膜的效果。进而通过转录组和WGCNA分析揭示了其中的关键调控模块及分子网络。裂解酶TSPphg显示了良好的金黄色葡萄球菌生物被膜抑制效果,解析出了该过程中的差异基因、特征性调控模块及核心因子网络,为抗生素替代、生物被膜的清除提供了新的思路。

关键词：生物被膜金黄色葡萄球菌噬菌体裂解酶加权共表达网络分析(WGCNA) 代谢通路核心驱动因子

Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2019年第5期16卷 1121-1134页

作者： GU Yun-fu LIU Ting bai Yan XIANG quan-ju ZHANG Xiao-ping CHEN Qiang Department of Microbiology College of Resource Science and Technology Sichuan Agricultural University

Amplicon sequencing of functional genes is a powerful technique to explore the diversity and abundance of microbes involved in biogeochemical processes. One such key process, denitrification, is of particular importance because it can transform nitrate(NO3-) to N2 gas that is released to the atmosphere. In nitrogen limited alpine wetlands, assessing bacterial denitrification under the stress of wetland desertification is fundamental to understand nutrients, especially nitrogen cycling in alpine wetlands, and thus imperative for the maintenance of healthy alpine wetland ecosystems. We applied amplicon sequencing of the nirS gene to analyze the response of denitrifying bacterial community to alpine wetland desertification in Zoige, China. Raw reads were processed for quality, translated with frameshift correction, and a total of 95,316 nirS gene sequences were used for rarefaction analysis, and 1011 OTUs were detected and used in downstream analysis. Compared to the pristine swamp soil, edaphic parameters including water content, organic carbon, total nitrogen, total phosphorous, available nitrogen, available phosphorous and potential denitrification rate were significantly decreased in the moderately degraded meadow soil and in severely degraded sandy soil. Diversity of the soil nirS-type denitrifying bacteria communities increased along the Zoige wetland desertification, and Proteobacteria and Chloroflexi were the dominant denitrifying bacterial species. Genus Cupriavidus(formerly Wautersia), Azoarcus, Azospira, Thiothrix, and Rhizobiales were significantly(P<0.05) depleted along the wetland desertification succession. Soil available phosphorous was the key determinant of the composition of the nirS gene containing denitrifying bacterial communities. The proportion of depleted taxa increased along the desertification of the Zoige wetland, suggesting that wetland desertification created specific physicochemical conditions that decreased the microhabitats for bacteria

关键词： Wetland desertification Amplicon sequencing nirS bacteria Differential abundance analysis

Oxidation behavior of artificial magnetite pellets

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2017年第6期24卷 603-610页

作者： Han-quan Zhang Jin-tao fu School of Resource and Civil Engineering Wuhan Institute of Technology Wuhan 430205 China Western Mining Company Xi’ning 810001 China

The oxidation behavior of artificial magnetite pellets was investigated through measurements of the oxidation degree and mineralogical analysis. The results show that artificial magnetite pellets are much easier to oxidize than natural magnetite. The oxidation is controlled through two different reaction mechanisms. The oxidation of artificial magnetite is dominated by internal diffusion, with an activation energy of 8.40 kJ/mol, at temperatures less than 800°C, whereas it is controlled by chemical reaction, with a reaction activation energy of 67.79 kJ/mol, at temperatures greater than 800°C. In addition, factors such as the oxygen volume fraction and the pellet diameter strongly influence the oxidation of artificial magnetite: a larger oxygen volume fraction and a smaller pellet diameter result in a much faster oxidation process.

关键词： artificial magnetite pellets oxidation reaction mechanisms

PRESSURE TRANSIENT ANALYSIS OF HETEROGENOUS RESERVOIRS WITH IMPERMEABILITY BARRIER USING PERTURBATION BOUNDARY ELEMENT METHOD

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2005年第1期17卷 102-109页

作者： YINHong-jun HEYing-fu fuChun-quan DepartmentofPetroleumEngineering DaqingPetroleumInstituteDaqing163318China InstituteofPorousflowandFluidMechanics ChineseAcademyofSciencesLangfang065007China

The transient flow mathematical model of arbitrary shaped heterogeneous reservoirs with impermeability barrier is proposed in this paper. In order to establish this model, the perturbation method is employed and the solution of model is expanded into a series in powers of perturbation parameter. By using the Boundary Element Method (BEM) and Duhamel principle, wellbore pressure with effects of skins and wellbore storage is obtained. The type curves are plotted and analyzed considering effects of heterogeneity, arbitrary shape and impermeable barriers. Finally, the results obtained by perturbation boundary element method is compared with the analytical solution and is available for the transient pressure analysis of arbitrary shaped reservoirs.

关键词： heterogeneous reservoirs arbitrary shape impermeable barrier perturbation equation boundary element method pressure transient analysis type curves