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Informative ISSR Markers Help Identify Genetically Distinct Accessions of Oryza rufipogon in Yield Improvement

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Rice science 2016年第5期23卷 225-241页

作者： G.HARITHA T.SUDHAKAR D.CHANDRA T.RAM b.divya N.SARLA ICAR Indian Institute of Rice Research

Inter simple sequence repeat（ISSR） polymorphism was used to determine genetic diversity and phylogenetic relationships in 90 genotypes of wild and cultivated species of Oryza from different geographical regions of the world. In all the 17 primers used in ISSR-PCR, a total of 11 464 bands were amplified at 253 band positions/loci. The primer UbC-809 amplified the maximum bands（1 059） at 21 band positions. UbC-810 and UbC-835 amplified the minimum of 391 bands each at 7 and 14 band positions, respectively. The mean polymorphism information content ranged from 0.44 to 0.84 and resolving power ranged from 8.69 to 23.53. Un-weighted pair group method with arithmetic mean dendrogram and population structure based on the 17 primers separated all genotypes into 4 major clusters with a genetic similarity of 53%–100%. The first two clusters consisted of 30 O. rufipogon accessions each. In the third cluster, O. nivara and O. longistaminata grouped as one sub-cluster and all other O. nivara accessions and cultivars grouped as another sub-cluster. The fourth cluster had only five O. rufipogon accessions which can be a source of new genes. Four sub-populations were identified within O. rufipogon and two sub-populations within O. nivara at K = 7. A subset of six primers with high resolving power values were the most informative and grouped all genotypes almost similarly as the 17 primers did. Use of these six highly informative primers in ISSR-PCR is a cost effective and robust method for assessing genetic diversity in large germplasm collections of wild rice species.

关键词： Oryza wild species genetic diversity population structure inter simple sequence repeat

Yield Traits and Associated Marker Segregation in Elite Introgression Lines Derived from O. sativa × O. nivara

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Rice science 2018年第1期25卷 19-31页

作者： G.HARITHA b.P.M.SWAMY M.L.NAIK b.JYOTHI b.divya S.MALATHI N.SARLA Indian Council of Agricultural Research Indian Institute of Rice Research Plant breeding Genetics and Biotechnology Division International Rice Research Institute

Introgression lines(ILs)derived from interspecific crosses are a source of new genetic variability.A total of 55 ILs derived from two crosses Swarna×*** IRGC81848(population A)and Swarna×*** IRGC81832(population b)were characterized for yield and yield-related traits/*** of 103 simple sequence repeat(SSR)markers associated with yield-related QTLs was *** A showed an average of 12.6%homozygous *** alleles and population b showed 10.6%.Interestingly,three SSR markers,RM223,RM128 and RM517,showed conspicuous pattern of *** distribution of parental alleles at three loci RM223,RM128 and RM517 linked to yield-related traits was *** markers flanked to several yield-related ***223,flanking to qyld8.3,was heterozygous in almost all the 55 ILs except in IL10-3S and ***128 on chromosome 1 and RM517 on chromosome 3 were mutually exclusive in 46 out of 55 *** 46 ILs showed either of the marker allele RM128 or RM517 from *** but not ***166S had both RM128and RM517 from *** and the other ILs showed homozygous Swarna allele at RM517 except *** structure assigned the 55 ILs to three sub-populations based on their genomic ***65S,IL166S,IL248S,IL7K and IL250K showed high yields in multi-location trials,and IL248S was released for cultivation as DRRDhan 40.

关键词： introgression line heterozygosity Oryza nivara wild rice population structure yield simple sequence repeat

Haplotyping of Rice Genotypes Using Simple Sequence Repeat Markers Associated with Salt Tolerance

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Rice science 2016年第6期23卷 317-325页

作者： A.D.CHOWDHURY G.HARITHA T.SUNITHA S.L.KRISHNAMURTHY b.divya G.PADMAVATHI T.RAM N.SARLA ICAR-Indian Institute of Rice Research Division of Crop Improvement Central Soil Salinity Research Institute

Salt stress is a major problem in most of the rice growing areas in the world. A major QTLSaltol associated with salt tolerance at the seedling stage has been mapped on chromosome 1 in *** study aimed to characterize the haplotype diversity at Saltol and additional QTLs associated withsalt tolerance. Salt tolerance at the seedling stage was assessed in 54 rice genotypes in the scale of 1to 9 score at EC = 10 dSm^-1 under controlled environmental conditions. Seven new breeding linesincluding three KMR3/O. rufipogon introgression lines showed similar salt tolerant ability as FL478 andcan be good sources of new genes/alleles for salt tolerance. Simple sequence repeat （SSR） markerRM289 showed only two alleles and RM8094 showed seven alleles. Polymorphic information contentvalue varied from 0.55 for RM289 to 0.99 for RM8094 and RM493. based on 14 SSR markers, the 54lines were clearly separated into two major clusters. Fourteen haplotypes were identified based onSaltol linked markers with FL478 as the reference. Alleles of RM8094 and RM3412 can discriminatebetween the salt tolerant and susceptible genotypes clearly and hence can be useful in marker-assistedselection at the seedling stage. Other markers RM10720 on chromosome 1 and RM149 and RM264 onchromosome 8 can also distinguish tolerant and susceptible lines but with lesser stringency.

关键词： haplotype rice salt tolerance Saltol simple sequence repeat marker

A Hybrid Model for Improving Software Cost Estimation in Global Software Development

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Computers, Materials & Continua 2024年第1期78卷 1399-1422页

作者： Mehmood Ahmed Noraini b.Ibrahim Wasif Nisar Adeel Ahmed Muhammad Junaid Emmanuel Soriano Flores divya Anand Faculty of Computer Science and Information Technology Universiti Tun Hussein Onn Malaysia Parit RajaBatu Pahat86400Malaysia Department of Computer Science COMSATS University Wah Cantt CampusIslamabad47010Pakistan Department of Information Technology The University of HaripurKhyber Pakhtunkhwa22620Pakistan Engineering Research Innovation Group Universidad Europea del AtlanticoSantander39011Spain

Accurate software cost estimation in Global Software Development(GSD)remains challenging due to reliance on historical data and expert *** models,such as the Constructive Cost Model(COCOMO II),rely heavily on historical and accurate *** addition,expert judgment is required to set many input parameters,which can introduce subjectivity and variability in the estimation ***,there is a need to improve the current GSD models to mitigate reliance on historical data,subjectivity in expert judgment,inadequate consideration of GSD-based cost drivers and limited integration of modern technologies with cost *** study introduces a novel hybrid model that synergizes the COCOMO II with Artificial Neural Networks(ANN)to address these *** proposed hybrid model integrates additional GSD-based cost drivers identified through a systematic literature review and further vetted by industry *** article compares the effectiveness of the proposedmodelwith state-of-the-artmachine learning-basedmodels for software cost *** the NASA 93 dataset by adopting twenty-six GSD-based cost drivers reveals that our hybrid model achieves superior accuracy,outperforming existing *** findings indicate the potential of combining COCOMO II,ANN,and additional GSD-based cost drivers to transform cost estimation in GSD.

关键词： Artificial neural networks COCOMO II cost drivers global software development linear regression software cost estimation

Comparative utility of vestibular function tests in patients with peripheral and central vestibular dysfunction

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Journal of Otology 2024年第1期19卷 5-9页

作者： Cameron b.Fattahi Christopher Zaro Janice J.Chung Richard F.Lewis divya A.Chari Department of Otolaryngology-Head and Neck Surgery UMASS Memorial Medical CenterUniversity of Massachusetts Chan Medical SchoolWorcesterMAUSA Jenks Vestibular Physiology Laboratory Massachusetts Eye and EarBostonMAUSA Department of Otolaryngology-Head and Neck Surgery Massachusetts Eye and EarHarvard Medical SchoolBostonMAUSA Department of Neurology Harvard Medical SchoolBostonMAUSA

background: bithermal caloric irrigation, video head impulse test(vHIT), and rotational testing are commonly used to assess peripheral vestibular function, but the relative clinical utility of each test in differentiating patients with peripheral vestibulopathy is ***: To determine whether(1) the combination of two or more vestibular tests enhances diagnostic utility over a single test;(2) abnormal test results on vestibular tests correlate with one ***: Retrospective analysis of data collected from multidisciplinary vestibular clinics at two academic medical centers from 2016 to ***: 150 patients(54.10 ± 15.09 years, 88 females) were included. No individual test was significantly better at predicting the presence of peripheral vestibular damage(p > 0.05). vHIT test results improved significantly when combined with either the caloric test(p = 0.007) or rotary chair test(p = 0.039). Caloric and rotational testing had high sensitivity(74.65% and 76.06%, respectively) and specificity(83.54% and 78.48%, respectively). vHIT demonstrated excellent specificity(89.87%) but poor sensitivity(47.89%). Caloric, vHIT, and rotary chair tests results did not correlate with one another(p > 0.05).Conclusions: Vestibular function tests have comparable diagnostic utility, yet each offers unique advantages. Caloric and rotational testing may be best suited for screening peripheral damage and vHIT may function ideally as a confirmatory test.

关键词： vestibular patients specificity

Should we recycle the graphite from spent lithium-ion batteries?The untold story of graphite with the importance of recycling

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Journal of Energy Chemistry 2022年第8期31卷 351-369,I0010页

作者： Subramanian Natarajan Madhusoodhanan Lathika divya Vanchiappan Aravindan Department of Chemistry Indian Institute of Science Education and Research(IISER)

Demand for graphite in the forthcoming years to develop Li-ion batteries(LIbs)with the goal of driving electric vehicles(EV)and its requirement in multifarious energy storage applications as an *** emerging sector of LIb-based EVs,along with portable electronics,produces an inevitable volume of batteries in the e-waste *** main reason for the lower percentage of recycling(at present,ble in a massive amount of spent LIbs,are urgently needed to be updated to ensure the reuse of all *** approach lifts the recycling process to develop an economic one besides the geostrategic and environmental policy ***,we summarize the importance of graphite and its demand and specify the reasons to recycle the graphite from spent LIbs along with its development as an anode in ***,the approach of the current recycling process of graphite in lab-scale and industries for various applications,including energy storage,are discussed with the highlights of future progress.

关键词： Recycling Lithium-ion batteries Graphite Anode Capacitor

Geodetic Number and Geo-Chromatic Number of 2-Cartesian Product of Some Graphs

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Open Journal of Discrete Mathematics 2022年第1期12卷 1-16页

作者： Medha Itagi Huilgol b. divya Department of Mathematics Bengaluru City University Central College Campus Bengaluru India Department of Mathematics Bangalore University Bengaluru India

A set S ⊆ V (G) is called a geodetic set if every vertex of G lies on a shortest u-v path for some u, v ∈ S, the minimum cardinality among all geodetic sets is called geodetic number and is denoted by . A set C ⊆ V (G) is called a chromatic set if C contains all vertices of different colors in G, the minimum cardinality among all chromatic sets is called the chromatic number and is denoted by . A geo-chromatic set Sb>cb> ⊆ V (G) is both a geodetic set and a chromatic set. The geo-chromatic number of G is the minimum cardinality among all geo-chromatic sets of G. In this paper, we determine the geodetic number and the geo-chromatic number of 2-cartesian product of some standard graphs like complete graphs, cycles and paths.

关键词： Cartesian Product Grid Graphs Geodetic Set Geodetic Number Chromatic Set Chromatic Number Geo-Chromatic Set Geo-Chromatic Number

Nonlinear mixing-based terahertz emission in inclined rippled density plasmas

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Chinese Physics b 2023年第6期32卷 384-390页

作者： K Gopal A P Singh S divya Rajdhani College Department of Physics&ElectronicsUniversity of DelhiNew DelhiIndia Computational Plasma Dynamics Laboratory Rajdhani CollegeDepartment of Physics&ElectronicsUniversity of DelhiNew DelhiIndia

We propose to investigate the THz field generation using nonlinear mixing mechanism of laser beat wave with inclined rippled density *** laser pulses with frequencies(ω_(1),ω_(2)) and wave vectors(k_(1),k_(2)) co-propagate and resultant laser beat wave forms at beat frequency(ω_(1)-ω_(2)).Laser beat wave imparts a nonlinear force on the ambient electrons and pushes them outward with nonlinear velocity v_(NL).Coupling of induced density perturbation and nonlinear velocity v_(NL)generates nonlinear currents at laser beat frequency that further generates electromagnetic field E_((ω_(1)-ω_(2))) in terahertz(THz)*** the present scheme,density ripples are introduced at an angle with respect to laser propagation and flat Gaussian index(f) is introduced in laser field profile that transform curved top of Gaussian field envelope into flat top field *** combined effect of flat laser pulses with inclined density ripples in plasmas shows 10-fold enhancement in THz field amplitude when flat-Gaussian index(f) varies from 1 to ***,the THz field intensifies when density ripples inclination increases upto a certain angle and then decreases.

关键词： terahertz inclined ripple flat laser laser plasma interaction

The mediating role of sleep qualityon the relationship between internet gaming disorder and perceived stress and suicidal behaviour among Indian medical students

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General Psychiatry 2023年第3期36卷 175-188页

作者： Sunny Garg Aakanksha Kharb Deepika Verma Ritu Antil binika Khanna Ritika Sihag divya Lamba Psychiatry Bhagat Phool Singh Government Medical College for WomenKhanpur KalanSonipatHaryanaIndia MbbS Internship Bhagat Phool Singh Government Medical College for WomenKhanpur KalanSonipatHaryanaIndia MbbS Student Final Prof Bhagat Phool Singh Government Medical CollegeforWomenKhanpur KalanSonipatHaryanaIndia

background In the recent digital era,individuals with internet gaming disorder(IGD)have reported a much higher prevalence of poor sleep quality,perceived stress and suicidal ***,the underlying mechanisms for these psychological problems remain *** The primary aims of this study were to explore the mediating role of sleep quality on the relationship between IGD and the health outcomes of perceived stress and suicidal behaviour and to assess the prevalence and risk factors for IGD among medical *** A cross-sectional study enrolling 795 medical students from two medical colleges in a rural area of North India was conducted from April to May *** study participants were chosen using a stratified random sampling approach.A self-administered questionnaire was used to collect data,including sociodemographic and personal information and gaming *** study also included the Gaming Disorder and Hazardous Gaming Scale,the Pittsburgh Sleep Quality Index,the Perceived Stress Scale-10 and the Suicide behaviors Questionnaire-Revised to measure IGD,sleep quality,perceived stress and suicidal behaviour,*** logistic regression for the risk factors and Pearson's correlation test for the relationship between variables were ***'PROCESS macro for SPSS was employed to carry out mediation *** Among the 348 gamers with a mean age of 21.03(SD 3.27)years,the prevalence of IGD was 15.23%(95%confidence interval:11.6%to 19.4%).In the correlational analysis,small to large(r:0.32-0.72)significant relationships between scores of IGD and other health outcomes were *** indirect effect(b=0.300)via sleep quality accounted for 30.55%of the total effect(b=0.982)of IGD on perceived stress(partially mediated),while sleep quality(b=0.174)accounted for 27.93%of the total effect(b=0.623)of IGD on suicidal behaviour(partially mediated).The factors of being male,living in a single-parent family,using the inte

关键词： behaviour internet media