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Immunoglobulin G Specific Antibody Level against Ebola Viral Glycoprotein and Nucleoprotein in Ebola Virus Disease Survivors and Their relatives

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Journal of biosciences and Medicines 2021年第4期9卷 179-196页

作者： Nadège Goumkwa Mafopa raoul Emeric Guetiya Wadoum Marta Giovanetti Antonella Minutolo béatrice Dambaya Claude Kwe Yinda Gianluca russo Georges Teto Georgia Ambada Patrick Turay Judith torimiro Alexis Ndjolo Jules roger Kuate Vittorio Colizzi Carla Montesano Chantal bIYA International reference Centre for research on HIV/AIDS Prevention and Management Yaoundé Cameroon University of roma “tor Vergata” Rome Italy Department of biochemistry University of Dschang Dschang Cameroon Departement of Public Health University of Makeni Makeni Sierra Leone National Institute of Health—ISS Rome Italy Faculty of Science University of Yaoundé I Yaoundé Cameroon Intramural research Program National Institute of Health Bethesda Maryland USA University di roma “Sapienza” Rome Italy Holy Spirit Hospital Makeni Makeni Sierra Leone Faculty of Medicine and biomedical Sciences University of Yaoundé I Yaoundé Cameroon

Ebola virus disease is a complex zoonosis that is highly virulent in humans. Despite its sorely pathogenic and lethal nature, survivors of this infection and even asymptomatic cases are able to develop both humoral and cellular immunity against several Ebola virus (EbOV) proteins. We aimed at determining immunoglobulin G (IgG) antibodies level against two Ebola viral antigens, the glycoprotein and the nucleoprotein in Ebola survivors and their relatives. Anti-EbOV glycoprotein (GP) and nucleoprotein (NP) IgG antibodies were quantified using ELISA. We enrolled 199 participants in two different sites as follow: 91 survivors at the Loreto clinic and 70 survivors with 38 relatives of Sierra Leone Association of Ebola Survivors bombali branch (SLAesb) tested for anti-EbOV NP and anti-EbOV GP IgG antibodies. Our findings revealed that the median anti-EbOV IgG level among survivors was 5.7128 U/ml [IQr: 2.793 - 7.783] for anti-EbOV GP IgG and 4.431 U/ml [IQr: 2.083 - 7.696] for anti-EbOV NP IgG. Survivors relatives had a median anti-EbOV GP IgG level of ?0.7128 U/ml [IQr: -0.903 to -0.04327] and -2.711 U/ml [IQr: -4.01 to -1.918] for anti-EbOV NP IgG. We observed that IgG levels in survivors were higher than in relatives with a significant difference of about 0.0001. The median value of anti-EbOV IgG level among seropositive relatives was 0.7043 U/ml [IQr: 0.5686 to 3.716] for anti-EbOV GP IgG and 4.05 U/ml [IQr: 0.2765 to 7.759] for anti-EbOV NP IgG respectively. Interestingly, we observed that 3.30% of Loreto clinic survivors did not developed anti-EbOV NP IgG antibodies;also about 10% survivors of the SLAesb were not reactive to anti-EbOV NP IgG and 1.43% of these survivors did not express antibodies against the Ebola viral glycoprotein. Our work is consistent with previous published studies showing heterogeneity in both survivors and asymptomatic cases of Ebola infection developing adaptive immunity against EbOV proteins.

关键词： Immunoglobulin IgG Level Ebola Survivors and relatives Glycoprotein and Nucleoprotein

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嘉陵江典型曲流地质遗迹特征及综合开发--以南充嘉陵江曲流省级地质公园为例

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西华师范大学学报（自然科学版） 2018年第3期39卷 292-298页

作者：李卫朋熊江叶云佳文星跃游泳税攀恒西华师范大学国土资源学院四川南充637009 西华师范大学区域环境演变与保护研究中心四川南充637009 成都理工大学环境与土木工程学院成都610059 西华师范大学文学院四川南充637009

以青居河曲为主体的嘉陵江曲流,在科学研究、科普教育、旅游开发等方面具有较高价值,已建有嘉陵江曲流地质公园,具备国家级地质公园发展潜质。然而其地质遗迹资源价值尚未得以充分体现,公园客源市场乏力。通过SWOT分析对嘉陵江曲流地质... 详细信息

以青居河曲为主体的嘉陵江曲流,在科学研究、科普教育、旅游开发等方面具有较高价值,已建有嘉陵江曲流地质公园,具备国家级地质公园发展潜质。然而其地质遗迹资源价值尚未得以充分体现,公园客源市场乏力。通过SWOT分析对嘉陵江曲流地质遗迹及其地质公园开发条件进行了系统分析。结果表明该公园拥有区位条件、景观类型、美学价值等方面的优势;存在建设投入、特色产品、管理制度的不足;存在周边旅游资源的竞争、分流与屏蔽效应以及曲流自然—人为侵蚀与裁弯取直等威胁,同时拥有生态文明理念与政策支持、日益多元化的旅游需求、不断改善的通达条件等机遇。基于此,提出了推进公园建设与宣传力度、打造特色旅游资源、转变管理模式等策略,以期为公园后期深度开发与建设提供参考依据。

关键词：嘉陵江曲流地质公园青居曲流地质遗迹 SWOT分析综合开发

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Geometric and statistical properties of the mean-field hydrophobic-polar model, the large-small model, and real protein sequences

Geometric and statistical properties of the mean-field hydro...

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CCAST“蛋白质结构分析中的一些问题”研讨会

作者： C.T.Shih Z.Y.Su. J.F.Gwan b.L.Hao C.H.Hsieh J.L.Lo H.C.Lee National Center for High-Performance Computing Hstnchu National Center for High-Performance Computing Hstnchu Forum Modellierung Forschungszentrum Julich. Institute of Theoretical Physics. Academia Sinica National Center for High-Performance Computing Hstnchu Department of Physics and Department of Life Science. National Central University Chungli. Department of Physics and Department of Life Science.

＜正＞ Lattice models, for their coarse-grained nature, are best suited tor the study of the "designability problem," the phenomenon in which most of the about 16000 proteins of known structure have their native conformations concentrated in a relatively small number of about 500 topological classes of conformations. Here it is shown that on a lattice the most highly designable simulated protein structures are those that have the largest number of surface-core switchbacks. A combination of physical, mathematical, and biological reasons that causes the phenomenon is given. by comparing the most foldable model peptides with protein sequences in the Protein Data bank. it is shown that whereas different models may yield similar designabilities, predicted foldable peptides will simulate natural proteins only when the model incorporates the correct physics and biology. in this case if the main folding force arises from the differing hydrophobicity of The residues, but does not originate. say. from the sterie hindrance effect caused by the differing sizes of the residues.

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EXPErIMENTAL VIbrIO EL-tor INFECTED WATEr FISH brEEDING: STresSING resErVOIr esTAbLISHMENT IN LOACH AND FINLesS EELS

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中华医学杂志(英文版) 1987年第2期100卷 145-145页

作者： J b Wang M S Cheng J L Gao Z Y Fan Y W Hong T X Ye Sanitary and Anti-epidemic Station Wuhu District Wuhu Department of biology Anhui Normal University Wuhu Department of Microbiology 2nd Military Medical College Shanghai

Experimental raising of shellfish, amphibians and other fish in water artificially polluted with Vibrio el-tor was started in 1979. The results show that Vibrio el-tor can live in vat water in which both loaches and finless eels are raised 25 weeks despite water renewal every week. Autopsies of all fish showed that 12 of 47 loaches and 7 of 39 eels contained large numbers of Vibrio el-tor in their gall-bladders and intestines, which had caused no pathologic changes. This indicates that loaches and eels ingest the vibrios which then enter the gallbladders and intestines where they multiply and are then excreted into the water. The authors believe that both the loach and finless eel may be reservoirs of Vibrio el-tor, playing a role as a source of cholera infection. Even though our results are from a laboratory situation, they cast new light on the breeding ecology of V. el-tor, and aquatic reservoir transmission of cholera epidemiologically.

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单色准分子激光(308nm)治疗早期菌样肉芽肿的疗效观察

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 57-57页

作者： Nisticò S. Costanzo A. Saraceno r. Chimenti S. 党倩丽 Department of Dermatology Univ. of Rome Policlin. Tor Vergata Viale Oxford 81 00133 Rome I taly

background: Various reports have recently shown the efficacy of narrowband ul traviolet (UV) b phototherapy at 311 nm in the treatment of early stage mycosis fungoides (MF). Objectives: To examine the effectiveness and tolerability of mon ochromatic excimer light (MEL) at 308 nm as a first treatment for early stage MF (stage IA). Methods: Ten lesions from five patients with a clinical and histolo gical diagnosis of MF were treated with repeated applications of MEL until compl ete remission was achieved or up to a maximum of 10 applications, with a cumulat ive dose of 308 nm UVb of between 6 and 12 J cm- 2. All patients were observed every 2 weeks for 2 months, with a 1- year follow-up. results: At present, al l patients are in complete remission, with no side-effects. Conclusions: based on these results, MEL can be considered a useful tool in the treatment of early stage MF.

关键词：准分子激光组织病理诊断片皮临床疗效观察单

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Effects of water extracts of Davallia formosana on ovariectomized mice

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Chinese Herbal Medicines 2018年第1期10卷 66-72页

作者： chia-feng wu cheng-yu chen ming-chang wu jen-shinn lin department of food science national pingtung university of science and technologypingtung 91201china department of medicinal plant development yupintang traditional chinese medicine foundationkaohsiung 000800china

Objective： The Taiwan residents native fern, Davallia formosana, is used to treat bone diseases in traditional Chinese medicine, but, very few animal tests to verify. To study how this fern and its active components alter bone metabolism. Methods： The effects of low and high doses of D. formosana water extracts （DFW, 50 and 200 mg/（kg·d）） and （-）-epicatechin-3-O-D-allopyranoside isolates of DFW （ECAP, 0.06 and 2.5 mg/（kg·d）） on protein lev- els of important indicators of osteoclast and osteoblast activities were examined. Ovariectomized mice were used as a model of osteoporosis. bone morphogenetic protein 2 （bMP-2）, collagen 1 （COL-1）, alka- line phosphatase （ALP）, runt-related transcription factor 2 （rUNX2）, osteocalcin （OCN）, and osteopontin （OPN） were analyzed using western blotting of femur tissue. Interleukin （IL）-1β and tumor necrosis fac- tor （TNF）-a were measured in the serum, and hematoxylin and eosin staining were used to assess bone morphology. results： Treatment with DFW or ECAP significantly induced bMP-2, COL-l, ALP, rUNX2, OCN, and OPN protein expression, indicating stimulation of osteogenesis. The treatments also reduced IL-1β and TNF- expression, indicating inhibition of osteoclastogenesis. In histological examinations, mice treated with DFW or ECAP had more bone trabeculae. The results demonstrate that DFW and ECAP inhibit osteo- clast differentiation and promote osteoblast differentiation, and effectively ameliorate ovariectomy （OVX）- induced osteoporosis. Conclusion： ECAP is a major component of DFW, which may have therapeutic potential for the treatment of diseases associated with excessive osteoclastic and insufficient osteogenic activity. ECAP may not be the specific active molecule because it is unstable in the stomach. However, the major active molecule needs to be confirmed using a relevant pharmacological model.

关键词： Davallia formosana Hayata osteoporosis osteogenesis osteoclastogenesis

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天山冰川物质平衡波动和径流的同步监测

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冰川冻土 1992年第3期14卷 226-232页

作者： M.b.久尔格洛夫 V.N.米哈连科 M.G.库那克霍维奇 S.N.尤希努尔特斯夫刘潮海谢自楚张万昌王纯足原苏联科学院地理研究所中国科学院兰州冰川冻土研究所

为了研究冰川物质平衡和径流形成过程的同步性,1989年夏季在图尤克苏冰川(外伊犁阿拉套山)、萨雷托尔冰川(阿克什腊克山)和乌鲁木齐河1号冰川(天格尔山)开展了同步观测。融水渗浸作用和径流过程在西天山所有高度范围、在中央天山和东天... 详细信息

为了研究冰川物质平衡和径流形成过程的同步性,1989年夏季在图尤克苏冰川(外伊犁阿拉套山)、萨雷托尔冰川(阿克什腊克山)和乌鲁木齐河1号冰川(天格尔山)开展了同步观测。融水渗浸作用和径流过程在西天山所有高度范围、在中央天山和东天山下部地带均有发生。夏季,中央天山4800—5000m以上的山地持续雪的积累,而未形成表面径流。最大负物质平衡出现在西天山的北天山。在冰川负物质平衡的年份里,增补的融水注入河流,这一过程在西天山持续到8月上旬到9月底,而在东天山和中央天山则持续到8月末。

关键词：天山冰川物质平衡波动径流

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基于地理信息系统的四川省卫生资源配置空间分析

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西华师范大学学报（自然科学版） 2018年第3期39卷 299-305页

作者：刘芳徐亚莉唐富强罗秀李健柯雄西华师范大学管理学院四川南充637009 西华师范大学国土资源学院四川南充637009 川北医学院四川南充637007

应用Arc GIS 10.1软件,运用反距离加权(IDW)插值法、空间自相关分析和Getis-Ord Gi9)热点分析的研究方法,探讨2015年四川省卫生资源配置地理空间分布状况,为优化卫生资源配置提供参考。反距离加权(IDW)插值显示,卫生资源集中在成都、攀... 详细信息

应用Arc GIS 10.1软件,运用反距离加权(IDW)插值法、空间自相关分析和Getis-Ord Gi9)热点分析的研究方法,探讨2015年四川省卫生资源配置地理空间分布状况,为优化卫生资源配置提供参考。反距离加权(IDW)插值显示,卫生资源集中在成都、攀枝花地区,并以这些地区为中心向周边地区辐射,而川东北、川西北、凉山地区配置较少。全局空间自相关分析结果显示,床位和护士配置在区域范围内呈随机分布,执业(助理)医师的配置呈正相关性;局部空间自相关分析显示,每千人口卫生机构床位高—高聚集区集中在眉山市,低—低聚集区集中在达州市、广安市;每千人口执业(助理)医师不存在集聚性,呈随机分布;每千人口执业护士低—低聚集区集中在南充市、广安市、达州市。Getis-Ord Gi*热点分析显示,眉山市、资阳市为每千人口卫生机构床位数的"热点区",达州市为每千人口执业护士数的"冷点区",其它地区差异无统计学意义。可以看出,四川省卫生资源空间配置均衡性有待优化。在制定卫生规划时,应考虑到地理要素和空间分布,重视卫生资源配置差异性问题,加强对欠发达地区的投入,缩小地域间差异。

关键词：卫生资源地理信息系统空间分析

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提肌上全盆腔脏器去除术后阴道再造术

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世界核心医学期刊文摘（妇产科学分册） 2006年第12期2卷 41-41页

作者： rettenmaier M.A. Goldstein b.H. Micha J.P. brown Ⅲ J.V. 朱亮 Gynecologic Oncology Associates Hoag Cancer Center351 Hospital RoadSuite 507Newport BeachCA 92663United States

background:There are several available techniques for neovaginal reconstruction following exenterative gynecologic surgery. However,all methods are associated with prolonged operative time and increased morbidity. The Apogee and PerigeeTM vaginal vault and prolapse repair systems are innovative and minimally invasive procedures that may prove to be effective in controlling the levator defect and reconstructing the vagina in patients undergoing supra-levator pelvic exenteration. Case:We present a patient who underwent supra-levator total pelvic exenteration for treatment of recurrent squamous cell carcinoma of the cervix. Vaginal reconstruction was performed with the Apogee and Perigee systems utilizing the porcine mesh (InteXen) from American Medical Systems. The patient did well without any postoperative vaginal or small bowel complications. Conclusion:The Apogee and Perigee systems comprise an innovative technique for vaginal vault reconstruction and prolapse repair. These systems may prove useful in reconstruction of the pelvis following ultra-radical pelvic procedures for recurrent gynecologic malignancies.

关键词：盆腔脏器去除术阴道再造术提肌宫颈鳞状上皮癌阴道穹隆妇科恶性肿瘤美国医疗修复系统手术时

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电碳酸氢钠协同转运蛋白1(NbCe1)在小鼠皮质星形胶质细胞中的功能性表达依赖于S255-257并受mtor调节

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神经损伤与功能重建 2019年第7期14卷 F0003-F0003页

作者： Khakipoor S Giannaki M Theparambil SM Zecha J Küster b Heermann S Deitmer JW roussa E 杜一星(编译) Department of Molecular Embryology Institute for Anatomy and Cell Biology Faculty of Medicine University of Freiburg Albertstrasse 17 D-79104 Freiburg Germany Department of Neuroanatomy Institute for Anatomy and Cell Biology Faculty of Medicine University of Freiburg Albertstrasse 17 D-79104 Freiburg Germany Department of General Zoology FB Biology University of Kaiserslautern P.B. 3049 D-67653 Kaiserslautern Germany 不详

电碳酸氢钠协同转运蛋白1,NbCe1(SLC4A4)是星形胶质细胞中表达的主要碳酸氢根转运蛋白。它对碳酸氢根高度敏感,并且是细胞内、细胞外和突触pH值的主要调节剂,从而调节神经元兴奋性。尽管已知NbCe1的这些基本功能,然而NbCe1介导的星形胶... 详细信息

电碳酸氢钠协同转运蛋白1,NbCe1(SLC4A4)是星形胶质细胞中表达的主要碳酸氢根转运蛋白。它对碳酸氢根高度敏感,并且是细胞内、细胞外和突触pH值的主要调节剂,从而调节神经元兴奋性。尽管已知NbCe1的这些基本功能,然而NbCe1介导的星形胶质细胞对细胞外pH值变化反应的分子机制尚不明确。使用原代小鼠皮质星形胶质细胞培养,本研究探索长期细胞外代谢碱中毒对NbCe1调节的影响;并通过免疫印迹,表面蛋白的生物素化,使用H+敏感染料2,7-双(羧乙基)-5-(6)-羧基荧光素记录细胞内H+,和磷酸化蛋白质组学分析阐明该调节作用潜在的分子机制。结果显示代谢性碱中毒后NbCe1活性显著下调,而NbCe1的蛋白质丰度或表面表达并不受影响。在碱中毒期间,NbCe1活性下调时细胞内H+变化的速率在野生型星形胶质细胞中降低,但在NbCe1缺陷小鼠的皮质星形胶质细胞中不变。激活mtor信号传导能够拯救碱中毒诱导的NbCe1活性降低。此外,质谱分析揭示S255-257的组成型磷酸化,而突变分析揭示这些残基对NbCe1转运活性至关重要。本研究证明了一种通过调节NbCe1的功能表达而实现的新的mtor调节机制。该种机制不仅适用于星形胶质细胞,也适用于上皮细胞中的NbCe1。

关键词：星形胶质细胞癫痫生长因子 pH值

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