检索结果-南通市图书馆

脂质转移蛋白SiLTP1基因参与谷子耐盐响应初探

在线全文

学校读者我要写书评

暂无评论

作物学报 2025年第1期51卷 58-67页

作者：孟凡花刘敏沈傲刘炜山东省农业科学院农作物种质资源研究所山东济南250100 上海应用技术大学生态技术与工程学院上海201418

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁... 详细信息

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁迫过程。前期本课题组克隆到一个谷子中编码脂质转移蛋白的基因SiLTP1,本研究通过构建其原核表达载体和植物双元过表达载体,获得了该基因的原核表达蛋白及同源转化过表达阳性植株,经进一步筛选获得4个纯合株系。体外耐盐性试验显示, SiLTP1的原核表达蛋白具有一定耐盐性;转基因植株苗期耐盐生理指标测定显示,SiLTP1过表达植株在遭受盐胁迫时,植株体内MDA积累减少,抗氧化酶含量升高,可积累更少的过氧化氢,从而降低胁迫对植株的氧化损伤,显示基因过表达植株具有更好的耐盐性。本研究结果初步揭示, SiLTP1可正向调控谷子的耐盐性,其在谷子耐盐抗逆方面具有潜在功能,为耐盐谷子品种改良及新品种培育提供了理论基础和候选基因资源。

关键词：脂质转移蛋白 SiLTP1 盐胁迫谷子耐盐响应

Strategies for improving ductility of ordered intermetallics

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2016年第1期26卷 1-12页

作者： Z.b.jiao j.H.Luan C.T.liu Center for Advanced Structural Materials Department of Mechanical and Biomedical Engineering College of Science and Engineering City University of Hong Kong

Ordered intermetallics possess attractive high-temperature properties; however, low ductility and brittle fracture limit their use as engineering materials in many cases. This paper provides a comprehensive review on the recent progress in the development of ductile ordered intermetallics and summarizes the strategies used to improve the tensile ductility of ordered intermetallics, including control of ordered crystal structures,engineering grain-boundary structure and chemistry, eliminating environmental embrittlement, microstructure optimization, control of phase stability, and promoting transformation-/twining-induced plasticity. The basic ideas and related mechanisms underlying these ductilizing strategies are discussed. In addition, a brief mention of the current use of intermetallic alloys for structural and corrosion applications is made.

关键词： Ordered intermetallics Ductility Ductilizing strategy Microstructure Alloy design

Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating YAP1 and autophagy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 887-899页

作者： jianan Wang Yuanyuan Ran Zihan Li Tianyuan Zhao Fangfang Zhang juan Wang Zongjian liu Xuechai Chen beijing International Science and Technology Cooperation base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk b homolog 5(ALKbH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(YAP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of YAP1 promoted autophagy,indicating that the mutual regulation between YAP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through YAP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKbH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol YAP1 YTHDF2

Efficient and Stable Perovskite Solar Cells and Modules Enabled by Tailoring Additive Distribution According to the Film Growth Dynamics

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第2期17卷 387-400页

作者： Mengen Ma Cuiling Zhang Yujiao Ma Weile Li Yao Wang Shaohang Wu Chong liu Yaohua Mai Institute of New Energy Technology College of Physics&Optoelectronic EngineeringJinan UniversityGuangzhou 510632People’s Republic of China Key Laboratory of New Semiconductors and Devices of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632People’s Republic of China Guangdong Mellow Energy Co. LimitedYuanming RoadZhuhai 519075GuangdongPeople’s Republic of China

Gas quenching and vacuum quenching process are widely applied to accelerate solvent volatilization to induce nucleation of perovskites in blade-coating *** this work,we found these two pre-crystallization processes lead to different order of crystallization dynamics within the perovskite thin film,resulting in the differences of additive *** then tailor-designed an additive molecule named 1,3-bis(4-methoxyphenyl)thiourea to obtain films with fewer defects and holes at the buried interface,and prepared perovskite solar cells with a certified efficiency of 23.75%.Furthermore,this work also demonstrates an efficiency of 20.18%for the large-area perovskite solar module(PSM)with an aperture area of 60.84 cm^(2).The PSM possesses remarkable continuous operation stability for maximum power point tracking of T_(90)>1000 h in ambient air.

关键词： Gas quenching Additive distribution buried passivation blade coating Crystallization dynamics

DETERMINATION OF CREEP PROPERTIES OF THERMAL bARRIER COATING(TbC)SYSTEMS FROM THE INDENTATION CREEP TESTING WITH ROUND FLAT INDENTERS

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2004年第4期17卷 503-508页

作者： b.Zhao b.X.Xu j.liu Z.F.Yue School of Mechanics Civil Engineering and Architecture Northwestern Polytechnic University Xi'an710072 ChinaSchool of Mechanics Civil Engineering and Architecture Northwestern Polytechnic University Xi'an710072 ChinaSchool of Mechanics Civil Engineering and Architecture Northwestern Polytechnic University Xi'an710072 ChinaSchool of Mechanics Civil Engineering and Architecture Northwestern Polytechnic University Xi'an710072 China

Indentation creep behavior with cylindrical flat indenters on the thermal barrier coating (TbC) was studied by finite element method (FEM). On ike constant applied indentation creep stress, there is a steady creep rate for each case studied for different creep properties of the TbC system. The steady creep depth rate depends on the applied indentation creep stress and size of the indenters as well as the creep properties of the bond coat of the TbC and the substrate. The possibilities to determine the creep properties of a thermal barrier system from indention creep testing were discussed. As an example, with two different size indenters, the creep properties of bond coat of the TbC system can be derived by an inverse FEM method. This study not only provides a numerical method to obtain the creep properties of the TbC system, but also extends the application of indentation creep method with cylindrical flat indenters.

关键词： thermal barrier coating (TbC) system indention creep testing,finite element creep analysis determination of creep parameters,bond coat

Effect of base Material on Microstructure and Texture Evolution of a Ti–6Al–4V Electron-beam Welded joint

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2017年第5期30卷 499-504页

作者： G.Q.Wang Z.b.Zhao b.b.Yu j.R.liu Q.j.Wang j.H.Zhang R.Yang j.W.Li School of Materials Science and Engineering University of Science and Technology of China Institute of Metal Research Chinese Academy of Sciences Science and Technology on Power beam Processes Laboratory Beijing Aeronautical Manufacturing Technology Research Institute

The effect of base material（bM） on microstructure and crystallographic orientation evolution of a Ti–6Al–4V electron-beam welded joint was investigated. Meanwhile, the crystallographic orientation of prior b grains was studied by advanced electron backscattered diffraction data processing. The inhomogeneity of microstructure within welded joint was formed due to the different microstructures of bM. by comparing microstructure details of the welded joint, including microstructure morphology and crystallographic orientation with those of the base material, it can be found that both the microstructure morphology and crystallographic orientation of the EbW joint would be controlled by bM.

关键词： Electron-beam welding Titanium alloys Prior b orientation Microstructure Texture

AAV2-PDE6b restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi Qiu Mingzhu Yang Xiuxiu jin jingyang liu Weiping Wang Xiaoli Zhang jinfeng Han bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6b ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6b rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6b and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6b injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6b restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6b injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6b ***,bax/bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6b *** data suggest that AAV2-PDE6b-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6b ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

Human neural stem cell-derived extracellular vesicles protect against ischemic stroke by activating the PI3K/AKT/mTOR pathway

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3245-3258页

作者： jiayi Wang Mengke Zhao Dong Fu Meina Wang Chao Han Zhongyue Lv Liang Wang jing liu Stem Cell Clinical Research Center The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of Stem Cell and Precision Medicine DalianLiaoning ProvinceChina Institute(College)of Integrative Medicine Dalian Medical UniversityDalianLiaoning ProvinceChina

Human neural stem cell-derived extracellular vesicles exhibit analogous functions to their parental cells,and can thus be used as substitutes for stem cells in stem cell therapy,thereby mitigating the risks of stem cell therapy and advancing the frontiers of stem cell-derived *** lays a foundation for the development of potentially potent new treatment modalities for ischemic ***,the precise mechanisms underlying the efficacy and safety of human neural stem cell-derived extracellular vesicles remain unclear,presenting challenges for clinical *** promote the translation of therapy based on human neural stem cell-derived extracellular vesicles from the bench to the bedside,we conducted a comprehensive preclinical study to evaluate the efficacy and safety of human neural stem cell-derived extracellular vesicles in the treatment of ischemic *** found that administration of human neural stem cell-derived extracellular vesicles to an ischemic stroke rat model reduced the volume of cerebral infarction and promoted functional recovery by alleviating neuronal *** human neural stem cell-derived extracellular vesicles reduced neuronal apoptosis by enhancing phosphorylation of phosphoinositide 3-kinase,mammalian target of rapamycin,and protein kinase b,and these effects were reversed by treatment with a phosphoinositide 3-kinase *** findings suggest that human neural stem cell-derived extracellular vesicles play a neuroprotective role in ischemic stroke through activation of phosphoinositide 3-kinase/protein kinase b/mammalian target of rapamycin signaling ***,we showed that human neural stem cell-derived extracellular vesicles have a good in vivo safety ***,human neural stem cell-derived extracellular vesicles are a promising potential agent for the treatment of ischemic stroke.

关键词： behavior exosome extracellular vesicles ischemic stroke mammalian target of rapamycin(mTOR) middle cerebral artery occlusion neural stem cells neuronal apoptosis phosphoinositide 3-kinase(PI3K) protein kinase b(AKT)

Exosomes originating from neural stem cells undergoing necroptosis participate in cellular communication by inducing TSC2 upregulation of recipient cells following spinal cord injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3273-3286页

作者： Shiming Li jianfeng Li Guoliang Chen Tao Lin Penghui Zhang Kuileung Tong Ningning Chen Shaoyu liu Shenzhen Key Laboratory of bone Tissue Repair and Translational Research Department of Orthopedic SurgeryThe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdong ProvinceChina Department of Orthopedic Surgery The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedics and Traumatology Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedic Surgery The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedic Surgery The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

We previously demonstrated that inhibiting neural stem cells necroptosis enhances functional recovery after spinal cord *** exosomes are recognized as playing a pivotal role in neural stem cells exocrine function,their precise function in spinal cord injury remains *** investigate the role of exosomes generated following neural stem cells necroptosis after spinal cord injury,we conducted singlecell RNA sequencing and validated that neural stem cells originate from ependymal cells and undergo necroptosis in response to spinal cord ***,we established an in vitro necroptosis model using neural stem cells isolated from embryonic mice aged 16-17 days and extracted *** results showed that necroptosis did not significantly impact the fundamental characteristics or number of *** sequencing of exosomes in necroptosis group identified 108 differentially expressed messenger RNAs,104 long non-coding RNAs,720 circular RNAs,and 14 microRNAs compared with the control *** of a competing endogenous RNA network identified the following hub genes:tuberous sclerosis 2(Tsc2),solute carrier family 16 member 3(Slc16a3),and forkhead box protein P1(Foxp1).Notably,a significant elevation in TSC2 expression was observed in spinal cord tissues following spinal cord ***2-positive cells were localized around SRY-box transcription factor 2-positive cells within the injury ***,in vitro analysis revealed increased TSC2 expression in exosomal receptor cells compared with other *** assessment of cellular communication following spinal cord injury showed that Tsc2 was involved in ependymal cellular communication at 1 and 3 days post-injury through the epidermal growth factor and midkine signaling *** addition,Slc16a3 participated in cellular communication in ependymal cells at 7 days post-injury via the vascular endothelial growth factor and macrophage migration inhibitory factor signaling

关键词： cellular communication competing endogenous RNA exosomes Foxp1 necroptosis neural stem cells Slc16a3 spinal cord injury transcriptome sequencing Tsc2