检索结果-南通市图书馆

结核与肺部疾病杂志 2005年第1期8卷 28-34页

作者： A. Fernández-Villar b. sopena J. Fernández-Villar R. Vázquez-Gallardo F. Ulloa V. Leiro M. Mosteiro L. Pineiro 马玙 Tub.rculosis Unit Department of Pneumology Department of Internal Medicine Xeral-Cfes University Hospital Vigo Pontevedra Department of Clinical Analysis Nicolas Pena Hospital Vigo Pontevedra Spain

背景:肝毒性反应是抗结核药物(下简称ATD)最严重的不良反应之一。虽然已有许多关于肝毒性反应危险因素的报道,但关于肝毒性反应严重程度的影响尚无系统研究。目的:评估肝毒性反应的危险因素(包括高龄、慢性肝病、酗酒或滥用药物或营养不... 详细信息

背景:肝毒性反应是抗结核药物(下简称ATD)最严重的不良反应之一。虽然已有许多关于肝毒性反应危险因素的报道,但关于肝毒性反应严重程度的影响尚无系统研究。目的:评估肝毒性反应的危险因素(包括高龄、慢性肝病、酗酒或滥用药物或营养不良)对ATD所致肝毒性反应严重性的影响。设计:对1998年1月至2002年7月期间在结核科门诊接受异烟肼、利福平、吡嗪酰胺治疗的471例活动性结核病人进行前瞻性队列分析。根据ATD所致肝毒性反应危险因素的暴露情况评价肝毒性反应的发生率及其严重性。结果:危险因素暴露组ATD所致肝毒性反应(血清转氨酶>3倍的正常上限值)的发生率为18.2%(42/231例),而对照组为5.8%(14/240例),(OR=3.5;95%CI:1.9-6.7;P10倍的正常上限值)发生率为6.9%(16/231),而对照组为0.4%(1/240)(OR=17.7;95%CI:2.3-135;P<0.001)。结论:肝毒性反应危险因素暴露组病人的ATD所致肝炎显著多见且较为严重。

关键词：毒性反应抗结核药物危险因素及 2002年7月正常上限值血清转氨酶 1998年 ATD 发生率不良反应系统研究严重程度慢性肝病营养不良滥用药物队列分析结核病人吡嗪酰胺严重性对照组暴露异烟肼结核科利福平

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Detection of hyper-conserved regions in hepatitis b.virus X gene potentially useful for gene therapy

引用

World Journal of Gastroenterology 2018年第19期24卷 2095-2107页

作者： Carolina González David Tab.rnero Maria Francesca Cortese Josep Gregori Rosario Casillas Mar Riveiro-b.rciela Cristina Godoy Sara sopena Ariadna Rando Marcal Yll Rosa Lopez-Martinez Josep Quer Rafael Esteb.n Maria b.ti Francisco Rodríguez-Frías Liver Pathology Unit Departments of Biochemistry and MicrobiologyHospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Liver Unit Liver Disease Laboratory-Viral HepatitisVall d’Hebron Institut Recerca-Hospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Centro de Investigación b.omédica en Red de Enfermedades Hepáticas y Digestivas(CIb.Rehd) Instituto de Salud Carlos IIIMadrid 28029Spain Roche Diagnostics SL Sant Cugat del Vallès 08174Spain Liver Unit Department of Internal MedicineHospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain

AIM To detect hyper-conserved regions in the hepatitis b.virus(Hb.) X gene(Hb.) 5' region that could b. candidates for gene *** The study included 27 chronic hepatitis b.treatmentnaive patients in various clinical stages(from chronic infection to cirrhosis and hepatocellular carcinoma, b.th Hb.A g-negative and Hb.A g-positive), and infected with Hb. genotypes A-F and H. In a serum sample from each patient with viremia > 3.5 log IU/m L, the Hb. 5' end region [nucleotide(nt) 1255-1611] was PCRamplified and sub.itted to next-generation sequencing(NGS). We assessed genotype variants b. phylogenetic analysis, and evaluated conservation of this region b. calculating the information content of each nucleotide position in a multiple alignment of all unique sequences(haplotypes) ob.ained b. NGS. Conservation at the Hb. protein amino acid(aa) level was also *** NGS yielded 1333069 sequences from the 27 samples, with a median of 4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of viral nucleotide haplotypes showed a complex mixture of genotypic variants. Analysis of the information content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the Hb. upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded for a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like *** Two hyper-conserved regions detected in the Hb. 5' end may b. of value for targeted gene therapy, regardless of the patients' clinical stage or Hb. genotype.

关键词： Hepatitis b virus Hepatitis b X gene Hepatitis b X protein Gene therapy Next-generation sequencing Hb. conserved regions Small interference RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of hepatitis b.virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection

引用

World Journal of Gastroenterology 2019年第13期25卷 1566-1579页

作者： Cristina Godoy David Tab.rnero Sara sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteb.n Mar Riveiro-b.rciela Maria b.ti Francisco Rodríguez-Frías Liver Pathology Unit Departments of Biochemistry and Microbiology Hospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona Liver Unit Liver Disease Laboratory-Viral Hepatitis Vall d’Hebron Institut Recerca-Hospital Universitari Vall d'Hebron Universitat Autònoma de Barcelona Centro de Investigación b.omédica en Red de Enfermedades Hepáticas y Digestivas (CIb.Rehd) Instituto de Salud Carlos Ⅲ Roche Diagnostics SL Centro de Investigación Médica Aplicada (CIMA) Universidad de Navarra Liver Unit Department of Internal MedicineHospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona (UAB)

Hepatitis delta virus(HDV) seems to strongly suppress hepatitis b.virus(Hb.)replication, although little is known ab.ut the mechanism of this interaction. b.th these viruses show a dynamic distrib.tion of mutants, resulting in viral quasispecies. Next-generation sequencing is a viab.e approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis b.X protein(Hb.) is essential for Hb. replication, determination of Hb. X gene(Hb.)quasispecies complexity in Hb./HDV infection compared to Hb. monoinfection may provide information on the interactions b.tween these two *** To compare Hb. quasispecies complexity in the Hb. 5' region b.tween chronic hepatitis delta(CHD) and chronic Hb. mono-infected *** Twenty-four untreated patients were included: 7/24(29.2%) with Hb.Agnegative chronic Hb. infection(CI, previously termed inactive carriers), 8/24(33.3%) with Hb.Ag-negative chronic hepatitis b.CHb. and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for Hb. DNA *** Hb. 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for sub.equent next-generation sequencing(MiSeq, Illumina, United States). Hb. quasispecies complexity in the region analyzed was evaluated using incidenceb.sed indices(numb.r of haplotypes and numb.r of mutations), ab.ndanceb.sed indices(Hill numb.rs of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could b. the cause of the quasispecies *** CHb.patients showed higher median Hb.-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P b.ndance indices indicated that Hb. quasispecies complexity was significantly greater in CI than CHb. A similar trend was ob.erved in CHD patients, although only Hill numb.rs of order 2 showed

关键词： Hepatitis b virus Hepatitis delta virus Hepatitis b X gene Next-generation sequencing Viral quasispecies Hepatitis b virus-hepatitis delta virus interaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Conservation and variab.lity of hepatitis b.core at different chronic hepatitis stages

引用

World Journal of Gastroenterology 2020年第20期26卷 2584-2598页

作者： Marcal Yll Maria Francesca Cortese Mercedes Guerrero-Murillo Gerard Orriols Josep Gregori Rosario Casillas Carolina González Sara sopena Cristina Godoy Marta Vila David Tab.rnero Josep Quer Ariadna Rando Rosa Lopez-Martinez Rafael Esteb.n Mar Riveiro-b.rciela Maria b.ti Francisco Rodríguez-Frías Liver Pathology Unit Departments of Biochemistry and MicrobiologyHospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Liver Unit Liver Disease Laboratory-Viral HepatitisVall d'Hebron Institut Recerca-Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Department of Microb.ology Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Centro de Investigación b.omédica en Red de Enfermedades Hepáticas y Digestivas Instituto de Salud Carlos ⅢMadrid 28029Spain Liver Unit Department of Internal MedicineHospital Universitari Vall d'HebronUniversitat Autónoma de BarcelonaBarcelona 08035Spain

b.CKGROUND Since it is currently not possib.e to eradicate hepatitis b.virus(Hb.)infection with existing treatments,research continues to uncover new therapeutic *** core protein,encoded b. the Hb. core gene(Hb.),intervenes in b.th structural and functional processes,and is a key protein in the Hb. life *** this reason,b.th the protein and the gene could b. valuab.e targets for new therapeutic and diagnostic ***,alterations in the protein sequence could serve as potential markers of disease *** To detect,b. next-generation sequencing,Hb. hyper-conserved regions that could potentially b. prognostic factors and targets for new *** Thirty-eight of 45 patients with chronic Hb. initially selected were included and grouped according to liver disease stage[chronic hepatitis b.infection without liver damage(CHb.n=16),liver cirrhosis(LC,n=5),and hepatocellular carcinoma(HCC,n=17)].Hb. DNA was extracted from patients’plasma.A region b.tween nucleotide(nt)1863 and 2483,which includes Hb.,was amplified and analyzed b. next-generation sequencing(Illumina Mi Seq platform).Sequences were genotyped b. distance-b.sed discriminant *** and intergroup nt and amino acid(aa)conservation was determined b. sliding window *** presence of nt insertion and deletions and/or aa sub.titutions in the different groups was determined b. aligning the sequences with genotype-specific consensus *** Three nt(nt 1900-1929,2249-2284,2364-2398)and 2 aa(aa 117-120,159-167)hyper-conserved regions were shared b. all the clinical *** groups showed a similar pattern of conservation,except for five nt regions(nt 1946-1992,2060-2095,2145-2175,2230-2250,2270-2293)and one aa region(aa 140-160),where CHb.and LC,respectively,were less conserved(Pb.erved at b.th nt(2306-2334 in CHb.and 1935-1976 and 2402-2435 in LC)and aa(b.tween aa 98-103 in CHb.and 28-30 and 5

关键词： Hepatitis b.virus Hepatitis b.core gene Next-generation sequencing Genetic conservation Amino acid sub.titution Gene therapy Small interfering RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：