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Far.er.Par.icipator. Evaluation of Nur.er. b.d Tr.atments for.the Contr.l of r.ot-Knot Nematodes [Meloidogynejavanica （Tr.ub. Chitwood and Meloidogyne incognita （Kofoid and White） Chitwood] in Smallholder.Far.s in Kenya

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Jour.al of Agr.cultur.l Science and Technology(A) 2011年第5期1卷 647-662页

作者： D. K. Kar.nja S. r. Gowen b. Ndung u r. O. Museb. S. Simons A McLeod b. r. kerry P. K. Kar.nja b. Pemb.oke CAb.Inter.ational P.O. Box 633-00621 Nairobi Kenya School of Agr.cultur. Policy and Development University of Reading Reading RG6 6dR Berkshire UK Kenya Agr.cultur.l r.sear.h Institute - National Hor.icultur.l r.sear.h Centr. P.O. Box 220-01000 Thika Kenya r.thamsted r.sear.h Harpenden Hertfordshire AL5 2JQ UK

r.ot-knot nematodes (Meloidogyne spp.) constr.in the pr.duction of high value vegetab.e cr.ps, such as tomato, on smallholder.far.s in Kenya. For.sever.l year., contr.l of r.ot-knot nematodes lar.ely depended on the use of synthetic nematicides, most of which have b.en b.nned due to their.toxicity to humans and the envir.nment. Far.er.par.icipator. tr.als wer. estab.ished, for.two consecutive gr.wing seasons on far.er.’ fields infested with r.ot-knot nematodes (Meloidogyne javanica and Meloidogyne incognita), at two sites in centr.l Kenya (Mar.gua and Kir.nyaga distr.cts), r.pr.senting two contr.sting gr.up of far.er. (low-input and high-input). The tr.als aimed at evaluating the potential of using b.ological contr.l agents (b.As), i. e. Pochonia chlamydospor.a and Pasteur.a penetr.ns, antagonistic plants (Cr.talar.a gr.hamiana and Tagetes minuta), "tr.sh b.r.ing" and chemical contr.l (dazomet 98% = b.samid? Gr.nular. in the management of r.ot-knot nematodes in tomato nur.er. b.ds, and quantify the associated net b.nefits, compar.d to an untr.ated contr.l. Cr.talar.a gr.hamiana, "tr.sh b.r.ing" and dazomet, significantly (P r.duced the numb.r.of second-stage juveniles (J2s) of the r.ot-knot nematodes in the soil in nur.er. b.ds dur.ng the fir.t season at Kar.gui-ini, b.t ther. was no significant (P > 0.05) incr.ase in yield after.tr.nsplanting tomato seedlings fr.m the r.spective tr.atment. Similar.y, one of the b.As (P. chlamydospor.a), was effectively tr.nsfer.ed to the field with seedlings fr.m the b.A-tr.ated nur.er. b.ds, b.t ther. was no significant (P > 0.05) incr.ase in yield due to slow pr.lifer.tion of the b.A, after.tr.nsplanting. Par.icipator. par.ial b.dgets wer. successfully developed with smallholder.far.er., and pr.ved to b. a vital tool in making infor.ed decisions on management options for.r.ot-knot nematodes. In gener.l, lab.ur.was consider.d b. all far.er. as the major.input that contr.b.ted to the over.ll additional cost of the differ.nt tr.atments at b.th sites. This necessitates imp

关键词：南方根结线虫生物控制剂育苗床肯尼亚农民治疗农场劳动力资源

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A r.pr.ducib.e and sensitive method for.gener.ting high-quality tr.nscr.ptomes fr.m single whitefly salivar. glands and other.low-input tissues

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Insect Science 2022年第5期29卷 1318-1328页

作者： Mar.o Geb.ola b.andon H.Le kerry EMauck Depar.ment of Entomology University of California RiversideRiversideCalifornia Depar.ment of b.tany and Plant Sciences University of California RiversideRiversideCalifornia Institute of Integr.tive Genome b.ology University of California RiversideRiversideCaliforniaUSA

Tr.nscr.ptomic studies ar. an impor.ant tool for.under.tanding the molecular.pathways under.ying host plant use b. agr.cultur.l pests,including vector. of damaging plant pathogens.Thus far.b.lk r.A-Seq has b.en the main appr.ach for.non-model insects.This method r.lies on pooling lar.e numb.r. of whole or.anisms or.hundr.ds of individually dissected or.ans.The latter.appr.ach is logistically challenging,may intr.duce ar.ifacts of handling and stor.ge,and is not compatib.e with b.ological r.plication.Her.,we tested an appr.ach to gener.te tr.nscr.ptomes of individual salivar. glands and other.low-input b.dy tissues fr.m whiteflies(b.misia tab.ci MEAM1),which ar. major.vector. of plant vir.ses.b. compar.ng our.outputs to pub.ished b.lk r.A-Seq datasets for.whole whitefly b.dies and pools of salivar. glands,we demonstr.te that this appr.ach r.cover. similar.numb.r. of tr.nscr.pts r.lative to b.lk r.A-Seq in a tissue-specific manner.and for.some metr.cs,exceeds per.or.ance of b.lk tissue r.A-Seq.Lib.ar.es gener.ted fr.m individual salivar. glands also yielded additional novel tr.nscr.pts not identified in pooled salivar. gland datasets,and had hundr.ds of enr.ched tr.nscr.pts when compar.d with whole head tissues.Over.ll,our.study demonstr.tes that it is feasib.e to pr.duce high quality,r.plicated tr.nscr.ptomes of whitefly salivar. glands and other.low-input tissues.We anticipate that our.appr.ach will expand hypothesis-dr.ven r.sear.h on salivar. glands of whiteflies and other.Hemipter.,thus enab.ing novel contr.l str.tegies to disr.pt feeding and vir.s tr.nsmission.

关键词： Aleyr.didae b.misia tab.ci b.lk r.A-Seq insect vector. low-input r.A-Seq plant pathogens

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Congestive hear. failur. and sepsis:a r.tr.spective study of hospitalization outcomes fr.m a r.r.l hospital in Southwest Missour.

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Emer.ency and Cr.tical Car. Medicine 2022年第3期2卷 105-108页

作者： Alexandr. Skovr.n Mason Hinke Shelly N.b.Sloan Gr.g Stahl kerry Johnson Scott Goade r.b.r. Ar.ce Kansas City Univer.ity–College of Medicine JoplinMOUSA Fr.eman Health System JoplinMOUSA Missour. Souther. State Univer.ity JoplinMOUSA

b.ckgr.und:Sepsis is the leading cause of death in hospitalized patients and significant effor. has b.en made to facilitate ear.y diagnosis and management.However.aggr.ssive tr.atment can have negative effects,especially in patients with unstab.e volume status,such as those with congestive hear. failur..Methods:We used electr.nic medical r.cor.s to per.or. a r.tr.spective study looking at hospital outcomes in patients fr.m Southwest Missour. who wer. admitted with sepsis and had a comor.0}d diagnosis of hyper.ensive hear. failur..Our.pr.mar. outcome was in-hospital mor.ality.r.sults:We studied a total of 184 patients with the diagnosis of hyper.ensive hear. failur. with sepsis,and 348 patients with the diagnosis of sepsis that ser.ed as the b.seline gr.up.Ther. was a total of 37(20%)deaths in the hyper.ensive hear. failur. gr.up and a total of 94(27%)deaths in the b.seline gr.up.Conclusion:Our.study showed no significant differ.nce b.tween the b.seline gr.up and those with hyper.ensive hear. disease with hear. failur..

关键词： Congestive hear. failur. Hyper.ension Hyper.ensive hear. disease Sepsis

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Compliance with SEP-1 guidelines is associated with impr.ved outcomes for.septic shock b.t not for.sever. sepsis

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Jour.al of Intensive Medicine 2022年第3期2卷 167-172页

作者： Shelly Nb.loan Nate r.dr.guez Thomas Sewar. Lucy Sar. Lukas Moor. Gr.g Stahl kerry Johnson Scott Goade r.b.r. Ar.ce Depar.ment of Pr.mar. Car. College of MedicineKansas City University2901St.Johns Blvd.JoplinMO 64804USA Depar.ment of Quality Impr.vement Freeman Health SystemJoplinMO 64804USA Depar.ment of Mathematics Missouri Southern State UniversityJoplinMO 64801USA Depar.ment of Phar.acy Freeman Health SystemJoplinMO 64804USA

b.ckgr.und: In 2018, the Center. for.Medicaid and Medicar. Ser.ices (CMS) issued a pr.tocol for.the tr.atment of sepsis. This b.ndle pr.tocol, titled SEP-1 is a multicomponent 3 h and 6 h r.suscitation tr.atment for.patients with the diagnosis of either.sever. sepsis or.septic shock. The SEP-1 b.ndle includes antib.otic administr.tion, fluid b.lus, b.ood cultur.s, lactate measur.ment, vasopr.ssor. for.fluid-r.fr.ctor. hypotension, and a r.evaluation of volume status. We per.or.ed a r.tr.spective analysis of patients diagnosed with either.sever. sepsis or.septic shock compar.ng mor.ality outcomes b.sed on compliance with the updated SEP-1 b.ndle at a r.r.l community hospital. Methods: Mor.ality outcome and r.admission data wer. extr.cted fr.m an electr.nic medical r.cor.s datab.se fr.m Januar. 1, 2019, to June 30, 2020. Inter.ational Classification of Diseases (ICD)-10 codes wer. used to identify patients with either.sever. sepsis or.septic shock. Once identified, patients wer. separ.ted into four.populations: patients with sever. sepsis who met SEP-1, patients with sever. sepsis who failed SEP-1, patients with septic shock who met SEP-1, and patients with septic shock who failed SEP-1. A patient who met b.ndle cr.ter.a (SEP-1 cr.ter.a) r.ceived each component of the b.ndle in the time allotted. Using chi-squar.d test of homogeneity, mor.ality outcomes for.population pr.por.ions wer. investigated. Two sample pr.por.ion summar. hypothesis test and 95% confidence inter.als (CI) deter.ined significance in mor.ality outcomes. r.sults: Out of our.1122 patient population, 437 patients qualified to b. measur.d b. CMS cr.ter.a. Of the 437 patients, 195 met the tr.atment b.ndle and 242 failed the tr.atment b.ndle. Upon compar.ng the two gr.ups, we found the pr.b.b.e differ.nce in mor.ality r.te b.tween the met(14.87%) and failed b.ndle(27.69%) gr.ups to b. significant(95% CI: 5.28-20.34, P = 0.0013). However. the dr.ving for.e of this r.sult lies in the sub.r.up of patients with sever. sepsis with septic shock, which show

关键词： SEP-1 Sepsis Ear.y goal-dir.cted ther.py Center. for.Medicaid and Medicar. Ser.ices Quality impr.vement r.imb.r.ement

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The Par. Count Tool (PaCT) for.Design Concept Selection

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Moder. Mechanical Engineer.ng 2011年第2期1卷 13-24页

作者： Tar.ng Par.shar.kerry Poppa Katie Gr.ntham Lough r.b.r. b. Stone 不详

This paper.pr.sents a par. count tool that pr.dicts the par. count for.a par.icular.pr.duct concept dur.ng the conceptual design phase. The par. count tool will also aid in r.nking the design concepts b. the cr.ter.on of numb.r.of components for.a pr.duct. This tool utilizes existing automated concept gener.tion algor.thms to gener.te the design concepts. It extr.cts the availab.e data fr.m the Design Engineer.ng Lab.Design r.positor. to deter.ine an aver.ge numb.r.of par.s per.component type in the r.positor. and then calculates an aver.ge par. count for.new concepts. The par. count tool also uses an algor.thm to deter.ine how to connect two non-compatib.e components thr.ugh the addition of mutually compatib.e components. While emphasis is placed on the aver.ge par.s per.pr.duct in evaluating designs, the over.ll functional r.quir.ment of the pr.duct is also consider.d.

关键词： Mechanical Design Conceptual Design Design for Manufactur.ng and Assemb.y

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cffdr.: an r.package for.the Canadian For.st Fir. Danger.r.ting System

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Ecological Pr.cesses 2017年第1期6卷 30-40页

作者： Xianli Wang b.Mike Wotton Alan S.Cantin Mar.-Andr.Par.sien kerry Ander.on b.ett Moor. Mike D.Flannigan Gr.at Lakes For.str. Centr. Canadian Forest ServiceNatural Resources Canada1219 Queen Street EastSault Ste.MarieON P6A 2E5Canada Depar.ment of r.newab.e r.sour.es University of Alberta751 General Service BuildingEdmontonAB T6G 2H1Canada Faculty of For.str. University of Toronto33 Willcocks St.TorontoON M5S 3B3Canada Nor.her. For.str. Centr. Canadian Forest ServiceNatural Resources Canada5320-122nd StreetEdmontonAB T6H 3S5Canada

Intr.duction:The Canadian For.st Fir. Danger.r.ting System(CFFDr.)is a glob.lly known wildland fir. r.sk assessment system,and two major.components,the fir. weather.index system and the fir. b.havior.pr.diction system,have b.en extensively used b.th nationally and inter.ationally to aid oper.tional wildland fir. decision making.Methods:In this paper.we pr.sent an over.iew of an r.package cffdr.,which is developed to calculate components of the CFFDr.,and highlight some of its functionality.In par.icular.we demonstr.te how these functions could b. used for.lar.e data analysis.r.sults and Discussion:With this cffdr. package,we pr.vide a por.al for.not only a collection of r.functions dealing with all availab.e components in CFFDr. b.t also a platfor. for.var.ous additional developments that ar. useful for.the under.tanding of fir. occur.ence and b.havior.This is the fir.t time that all r.levant CFFDr. methods ar. incor.or.ted into the same platfor.,which can b. accessed b. b.th the management and r.sear.h communities.

关键词： Fir. b.havior.pr.diction system Fir. weather.index system For.st fir. For.st fir. r.sk Fuel moistur. Landscape ecology

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Ar.b.dopsis thaliana T-DNA Mutants Implicate GAUT Genes in the b.osynthesis of Pectin and Xylan in Cell Walls and Seed Testa

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Molecular.Plant 2009年第5期2卷 1000-1014页

作者： kerry H. Caffall Sivakumar.Pattathil Sar.h E. Phillips Michael G. Hahn Deb.a Mohnen Depar.ment of b.ochemistr. and Molecular.b.ology University of Georgia Athens GA 30602 USA Complex Car.0}hydr.te r.sear.h Center BioEnergy Science Center 315 Riverbend Road University of Georgia Athens GA 30602 USA Depar.ment of Plant b.ology University of Georgia Athens GA 30602 USA

Galactur.nosyltr.nsfer.se 1 （GAUT1） is an α1,4-D-galactur.nosyltr.nsfer.se that tr.nsfer. galactur.nic acid fr.m ur.dine 5＇-diphosphogalactur.nic acid onto the pectic polysacchar.de homogalactur.nan （Ster.ing et al., 2006）. The 25-memb.r.Ar.b.dopsis thaliana GAUT1-r.lated gene family encodes 15 GAUT and 10 GAUT-like （GATL） pr.teins with, r.spectively, 56-84 and 42-53% amino acid sequence similar.ty to GAUT1. Pr.vious phylogenetic analyses of AtGAUTs indicated thr.e clades： A thr.ugh C. A compar.tive phylogenetic analysis of the Ar.b.dopsis, poplar.and r.ce GAUT families has sub.classified the GAUTs into seven clades： clade A-1 （GAUTs 1 to 3）; A-2 （GAUT4）; A-3 （GAUTs 5 and 6）; A-4 （GAUT7）; b.1 （GAUTs 8 and 9）; b.2 （GAUTs 10 and 11）; and clade C （GAUTs 12 to 15）. The Ar.b.dopsis GAUTs have a distr.b.tion compar.b.e to the poplar.or.hologs, with the exception of GAUT2, which is ab.ent in poplar. r.ce, however. has no or.hologs of GAUTs 2 and 12 and has multiple appar.nt or.hologs of GAUTs 1, 4, and 7 compar.d with either.r.b.dopsis or.poplar. The cell wall glycosyl r.sidue compositions of 26 homozygous T-DNA inser.ion mutants for.13 of 15 Ar.b.dopsis GAUTgenes r.veal significantly and r.pr.ducib.y differ.nt cell walls in specific tissues of gaut mutants 6, 8, 9, 10, 11, 12, 13, and 14 fr.m that of wild-type Ar.b.dopsis walls. Pectin and xylan polysacchar.des ar. affected b. the loss of GAUT function, as demonstr.ted b. the alter.d galactur.nic acid, xylose, r.amnose, galactose, and ar.b.nose composition of distinct gaut mutant walls. The wall glycosyl r.sidue compositional phenotypes ob.er.ed among the gaut mutants suggest that at least six differ.nt b.osynthetic linkages in pectins and/or.xylans ar. affected b. the lesions in these GAUTgenes. Evidence is also pr.sented to suppor. a r.le for.GAUT11 in seed mucilage expansion and in seed wall and mucilage composition.

关键词： Car.0}hydr.te metab.lism cell walls Ar.b.dopsis b.osynthesis mutant pectin mucilage

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In vitr. C ytotoxic Pr.per.ies of Cr.de Polar.Extr.cts of Plants Sour.ed fr.m Austr.lia

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Clinical Complementar. Medicine and Phar.acology 2022年第1期2卷 13-22页

作者： Janice Mani Joel Johnson Holly Hosking kerry Walsh Paul Neilsen Mani Naiker School of Health Medical and Applied SciencesCentral Queensland UniversityBruce HwyNorth RockhamptonQueensland 4701Australia Institute of Futur. Far.ing Systems Central Queensland UniversityBruce HwyNorth RockhamptonQueensland 4701Australia

b.ckgr.und:Numer.us commer.ial phar.aceuticals-including anticancer.antivir.l,and antidiab.tic dr.gs have b.en developed fr.m tr.ditional plant-der.ved medicines.Ther. ar. appr.ximately 25,000 species of flor. occur.ing in Austr.lia,which ar. adapted to a r.nge of har.h envir.nments,and hence a plethor. of novel compounds ar. still awaiting r.sear.h in the context of their.medicinal pr.per.ies.Ob.ective:The cur.ent study ther.for. aimed to develop a systematic pr.tocol for.scr.ening plants with potential cytotoxicity.Many studies have found polar.compounds such as to b. the b.oactive components r.sponsib.e for.the ther.peutic effects.Methods:The total phenolic content(TPC)and antioxidant capacity(Fr.P)of methanolic extr.cts of selected plants was fir.t deter.ined.A high cor.elation b.tween the TPC values and Fr.P values of the plant polar.extr.cts wer. evident.Cell viab.lity of the cancer.cell lines wer. assessed using the MTS assay.r.sults:Cells sub.ected to Pittospor.m angustifolium Lodd.Extr.cts at concentr.tion of 250μg/mL showed no viab.e cells,compar.b.e to cisplatin(a chemother.py medication)used as a positive contr.l(10μg/mL).Some pr.mising inhib.tor. effects wer. also seen with Mur.ya koenigii flower.and leaves at concentr.tion of 250μg/mL,with only 43.46%and 63.88%cell viab.lity,r.spectively.In contr.st,extr.cts of Citr.s hystr.x(leaves)and Sygyz-ium austr.le(stamen)showed higher.per.entage cell viab.lities(ar.und 82-93%)at same concentr.tions.The phenolic pr.file of P.angustifolium b.extr.cts demonstr.ting gr.ater.peak intensity and dominant peaks wer. tentatively identified as chlor.genic acid,p-coumar.c acid,caffeic acid,t-fer.lic acid and r.tin.Conclusion:The Austr.lian species P.angustifolium extr.ct was the most cytotoxic against HeLa and HT29 cells compar.ng to the other.plant extr.cts tested.A HPLC pr.file of the P.angustifolium extr.ct also showed an ar.ay of pr.mising ther.peutic phenolic compounds.Futur. wor. will aim to fr.ctionate and isolate novel compounds fr.m this species and test their

关键词： Cytotoxic Antioxidant capacity Total phenolics Austr.lian plants Polar.extr.cts

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Endoscopic b.lloon catheter.dilatation via r.tr.gr.de or.static technique is safe and effective for.cr.cophar.ngeal dysfunction

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Wor.d Jour.al of Gastr.intestinal Endoscopy 2017年第4期9卷 183-188页

作者： Vinay Chandr.sekhar. Joyce Koh Lakshmi Lattimer.kerry b.Dunb.r.William J r.vich John O Clar.e Division of Gastr.enter.logy and Hepatology Department of Internal Medicinethe Johns Hopkins Medical Institutions Gastr.enter.logy Division Department of Internal MedicineUniversity of Pennsylvania Health System Per.lman Center.for.Advanced Medicine South Pavilion Gastr.enter.logy and Liver.Diseases Depart-ment of Internal Medicinethe George Washington University Division of Gastr.enter.logy and Hepatology Department of MedicineUniversity of Texas Southwestern Medical SchoolDallas VA Medical Center

AIM To evaluate the safety and efficacy of upper.esophageal sphincter.UES) dilatation for.cr.cophar.ngeal(CP) dysfunction. To deter.ine if:(1) indication for.dilatation; or.2) technique of dilatation cor.elated with symptom impr.vement.METHODS All b.lloon dilatations per.or.ed at our.institution fr.m over.a 3-year.per.od wer. r.tr.spectively analyzed for.demogr.phics, indication and dilatation site. All dilatations involving the UES under.ent fur.her.r.view to deter.ine efficacy, complications, and factor. that pr.dict success. Dilatation technique was separ.ted into static(stationar. b.lloon distention) and r.tr.gr.de(b.usque pull-b.ck of a fully distended b.lloon acr.ss the UES).r.SULTS Four.hundr.d and eighty-eight dilatations wer. r.viewed. Thir.y-one patients wer. identified who under.ent UES dilatation. Median age was 63 year.(r.nge 27-81)and 55% of patients wer. male. Indications included dysphagia(28 patients), glob.s sensation with evidence of UES dysfunction(2 patients) and ob.tr.ction to echocar.iogr.phy pr.b. with cr.cophar.ngeal(CP)b.r.1 patient). Ther. was evidence of concur.ent or.phar.ngeal dysfunction in 16 patients(52%) and a small Zenker.s diver.icula(≤ 2 cm) in 7 patients(23%). Dilator.size r.nged fr.m 15 mm to 20 mm. Of the 31 patients, 11 had dilatation of other.esophageal segments concur.ently with UES dilatation and 20 had UES dilatation alone.Follow-up was availab.e for.24 patients for.a median of 2.5 mo(inter.uar.ile r.nge 1-10 mo), of whom 19 r.por.ed symptomatic impr.vement(79%). For.patients under.oing UES dilatation alone, follow-up was availab.e for.15 patients, 12 of whom r.por.ed impr.vement(80%).Nineteen patients under.ent r.tr.gr.de dilatation(84%r.sponse) while 5 patients had static dilatation(60%r.sponse); however. ther. was no significant differ.nce in symptom impr.vement b.tween the techniques(P = 0.5).Successful symptom r.solution was also not significantly affected b. dilator.size, or.phar.ngeal dysfunction,Zenker.s diver.iculum, age or.gender.P > 0.05). The only complicat

关键词： Cr.cophar.geal 机能障碍 Cr.cophar.ngeal 酒吧咽下困难食道的膨胀内视镜的汽球膨胀

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Endoscopic options for.tr.atment of dysplasia in b.r.ett's esophagus

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Wor.d Jour.al of Gastr.intestinal Endoscopy 2015年第19期7卷 1311-1317页

作者： r.b.ooks Vance kerry b.Dunb.r VA Nor.h Texas Healthcar. System Dallas VA Medical Center University of Texas Southwestern Medical Center

r.cent advances in the endoscopic tr.atment of dysplasia in b.r.ett's esophagus(b.) have allowed endoscopists to pr.vide effective and dur.b.e er.dication ther.pies. This r.view summar.zes the availab.e endoscopic er.dication techniques for.dysplasia in patients with b. including endoscopic mucosal r.section, endoscopic sub.ucosal dissection, photodynamic ther.py, ar.on plasma coagulation, r.diofr.quency ab.ation and cr.other.py.

关键词： Dysplasia b.r.ett's esophagus Endoscopicther.py Endoscopic mucosal r.section r.diofr.quencyab.ation Endoscopy Photodynamic ther.py

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