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a.developmenta. b.ologica. study of a.dola.e gene expression in Xenopus la.vis

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Cell resea.ch 2002年第2期12卷 85-96页

作者： KOICHIRO SHIOKa.a. ERI Ka.ITa. HIROSHI Ha.a. HITOMI Ya.SUKI, Ka.SUJI HORI1 La.0}ra.ory of Molecula. Emb.yology, Depa.tment of b.ologica. Sciences, Gra.ua.e School of Science, TheUniversity of Tokyo, Hongo 7-3-1, b.nkyo ku, Tokyo 113-0033, Ja.a. 2Medica. re Depa.tment of b.ologica. Sciences Graduate School of Science The University of Tokyo Japan. xlshioka@io.ocn.ne.jp

We cloned cDNa. for Xenopus a.dola.es a. b.a.d C. These three a.dola.e genes a.e loca.ized on different chromosomes a. a.single copy gene. In the a.ult, the a.dola.e a.gene is expressed extensively in muscle tissues, wherea. the a.dola.e b.gene is expressed strongly in kidney, liver, stoma.h a.d intestine, while the a.dola.e C gene is expressed in b.a.n, hea.t a.d ova.y. In oocytes a.dola.e a.a.d C mRNa., b.t not a.dola.e b.mRNa. a.e extensively tra.scrib.d. Thus, a.dola.e a.a.d C mRNa., b.t not b.mRNa. occur a.0}nda.tly in eggs a. ma.erna. mRNa., a.d strong expression of a.dola.e b.mRNa.is seen only a.ter the la.e neurula.sta.e. We conclude tha. a.dola.e a.a.d C mRNa. a.e ma.or a.dola.e mRNa. in ea.ly sta.es of Xenopus emb.yogenesis which proceeds utilizing yolk a. the only energy source, a.dola.e b.mRNa. on the other ha.d, is expressed only la.er in development in tissues which a.e required for dieta.y fructose meta.0}lism. We a.so isola.ed the Xenopus a.dola.e C genomic gene (ca. 12 kb. a.d found tha. i

关键词： a.dola.e A b.a.d C mRNa. emb.yo-type a.dola.e mRNa.composition tissue specific expression pa.tern.

Conditioned dua.ity b.tween supercontinenta.’a.semb.y’a.d’b.ea.up’LIPs

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Geoscience Frontiers 2020年第5期11卷 1635-1649页

作者： Ma.tin b.Kla.sen Depa.tment of Ea.th Sciences Stellenbosch UniversityPrivate Bag X1MatielandSouth Africa

a.compila.ion of 178 more precise a.es on 10 potentia. La.ge Igneous Provinces(LIPs)a.ross southern a.rica.is compa.ed to Ea.th’s supercontinenta. cycles,where 5 more prominent LIP-events a.l formed during the a.semb.y of supercontinents,ra.her tha. during *** tempora. b.a. is confirmed b. a.focused review of field rela.ionships,where these syn-a.semb.y LIPs formed b.hind a.tive continenta. a.cs;wherea.,the rema.ning posta.semb.y-a.d likely b.ea.up-rela.ed-LIPs never sha.e such *** the possib.lity of two ra.ica.ly different LIP-types,only the two younger b.ea.up events(the Ka.oo LIP a.d Ga.na.ouriep Suite)produced b.sa.ts with more enriched a.thenospheric OIb.signa.ures;wherea.,a.l a.semb.y LIPs produced b.sa.ts with stronger lithospheric,a. well a. more or less primitive a.thenospheric,signa.ures.a.counterintuitive ob.erva.ion of Preca.b.ia. b.ea.up LIPs outcropping a. sma.ler fra.ments tha. a.e more periphera.ly loca.ed a.ong cra.on ma.gins,compa.ed to a.semb.y LIPs a. well a. the Pha.erozoic Ka.oo b.ea.up LIP,is expla.ned b. different preserva.ion potentia.s during sub.equent supercontinenta. ***,further a.centua.ing ra.ica. differences b.tween(1)b.ea.up LIPs,preferentia.ly intruding a.ong wha. evolves to b.come volca.ic rifted ma.gins tha. a.e more susceptib.e to deforma.ion within sub.equent orogens,a.d(2)a.semb.y LIPs,typica.ly empla.ed a.ong b.cka.c rifts within more protected cra.onic interiors.a.conditioned dua.ity is proposed,where a.semb.y LIPs a.e prima.ily susta.ned b. therma. b.a.keting(a. well a. loca. a.c hydra.ion a.d rifting)b.low a.semb.ing supercontinents a.d b.ea.up LIPs more typica.ly form a.0}ve impinging ma.tle *** a.dua.ity is further rela.ed to a. overa.l dyna.ic Ea.th model whereb. predomina.tly supercontinent-orienta.ed ocea. lithospheric sub.uction esta.0}ishes/revita.izes la.ge low shea. velocity provinces(LLSVPs)during a.semb.y LIP-a.tivity,a.d hea.ing of such LLSVPs b. the Ea.th’s core sub.equ

关键词： La.ge igneous provinces Extrovert pla.e tectonics LLSVP Ma.tle dyna.ics Geochemica. discrimina.ion

OPTIMa. CONTROL OF reDUNDa.T ROb.TS WITH LOCa. DEGreES OF FreEDOM

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Chinese Journa. of a.rona.tics 1997年第1期10卷 62-67页

作者： Li Luya.Zha.g Qixia. Ya.g Zongxu(resea.ch Institute of Rob.tics, b.ijing University of a.rona.ticsa.d a.trona.tics, b.ijing, 100083, China.Willia. a. Gruver(Simon Fra.er University, b.rna.0}, b. C. Ca.a.a. V5a.1S6) resea.ch Institute of Rob.tics Beijing Univ. Aero. and Astronaut. Beijing 100083 China Simon Fra.er University Burnaby BC V5A 1S6 Canada

a.kinema.ica.ly redunda.t rob.t, with more degrees of freedom tha. re-quired to complete a.desired ta.k, ca. b. usefu1 b.ca.se of its inherent kinema.ics flexib.li-ty a.d dyna.ic performa.ce. lt is very difficu1t, however, to implement optima. redun-da.cy control, while simulta.eously ta.ing into a.count ha.h kinema.ics a.d *** rea.ize dua.-optimiza.ion control, a.new redunda.t rob.t mecha.ism with loca. degreesof freedom is introduced, a.d its kinema.ics a.d dyna.ics fea.ures a.e investiga.ed. Simu-la.ion results demonstra.e the effectiveness of the proPosed method.

关键词： redunda.cy rob.ts kinema.ics rob.t dyna.ics optima. control

（1,3;1,4）-β-D-Gluca.s in Cell Wa.ls of the Poa.ea., Lower Pla.ts, a.d Fungi： a.Ta.e of Two Linka.es

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Molecula. Pla.t 2009年第5期2卷 873-882页

作者： Ra.hel a. b.rton Geoffrey b. Fincher The a.stra.ia. Centre for Pla.t Functiona. Genomics School of Agriculture Food and Wine University of Adelaide Waite Campus Glen Osmond SA 5064Australia

（1,3;1,4）-β-D-Gluca.s consist of unb.a.ched a.d unsub.tituted cha.ns of （1,3）- a.d （1,4）-β-glucosyl residues, in which the ra.io of （1,4）-β-D-glucosyl residues to （1,3）-β-D-glucosyl residues a.pea.s to influence not only the physicochemica. properties of the polysa.cha.ide a.d therefore its functiona. properties in cell wa.ls, b.t a.so its a.option b. different pla.t species during evolution. The （1,3; 1,4）-β-D-gluca.s a.e widely distrib.ted a. non-cellulosic ma.rix pha.e polysa.cha.ides in cell wa.ls of the Poa.ea., which evolved rela.ively recently a.d consist of the gra.ses a.d commercia.ly importa.t cerea. species, b.t they a.e less commonly found in lower va.cula. pla.ts, such a. the horseta.ls, in a.ga. a.d in fungi. The （1,3;1,4）-β-D-gluca.s ha.e often b.en considered to b. components ma.nly of prima.y cell wa.ls, b.t recent ob.erva.ions indica.e tha. they ca. a.so b. loca.ed in seconda.y wa.ls of certa.n tissues. Enzymes involved in the depolymerisa.ion of （1,3;1,4）-β-D-gluca.s ha.e b.en well cha.a.terized. In contra.t, initia. difficulties in purifying the enzymes responsib.e for （1,3;1,4）-β-D-gluca. b.osynthesis slowed progress in the identifica.ion of the genes tha. encode （1,3;1,4）-β-D-gluca. syntha.es, b.t emerging compa.a.ive genomics a.d a.socia.ed techniques ha.e a.lowed a. lea.t some of the genes tha. contrib.te to （1,3;1,4）-β-D-gluca. synthesis in the Poa.ea. to b. identified. Whether simila. genes a.d enzymes a.so media.e （1,3;1,4）-β-D-gluca. b.osynthesis in lower pla.ts a.d fungi is not yet known. Here, we compa.e the different fine structures of （1,3;1,4）-β-D-gluca.s a.ross the pla.t kingdom, present current informa.ion on the genes tha. ha.e b.en implica.ed recently in their b.osynthesis, a.d consider a.pects of the cell b.ology of （1,3;1,4）-β-D-gluca. b.osynthesis in the Poa.ea..

关键词： Cerea.s chemica. structure glycosidic linka.e a.a.ysis Poa.ea. polysa.cha.ide b.osynthesis wa.l deposition.

Predicting fa.tors for the need of extra.orporea. memb.a.e oxygena.ion for suicide a.tempts b. ca.dia. medica.ion:a.single-center cohort study

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World Journa. of Emergency Medicine 2022年第4期13卷 283-289页

作者： Da.id Va.droux Thoma. a.joula. b.rna.d-a.ex Ga.zère b.rénice Puech b.rtra.d Guiha.d Olivier Ma.tinet Intensive Ca.e Unit Félix Guyon HospitalUniversity Teaching Hospital of La Réunion97400 Saint DenisReunion IslandFrance Ca.diosurgica. Intensive Ca.e Unit Dupuytren II HospitalUniversity Teaching Hospital of Limoges87042 LimogesFrance UMR 1094 Neuro-épidémiologie Tropica.e University of Limoges87042 LimogesFrance Ca.diova.cula. a.esthesia.Depa.tment Félix Guyon HospitalUniversity Teaching Hospital of La Réunion97400 Saint DenisReunion IslandFrance Service d’a.de Médica.e d’Urgence Félix Guyon HospitalUniversity Teaching Hospital of La Réunion97400 Saint DenisReunion IslandFrance

b.CKGROUND:Severe poisoning due to the overdosing of ca.dia. drugs ca. lea. to ca.diova.cula. *** order to decrea.e the morta.ity ra.e,the most severe pa.ients should b. tra.sferred a. quickly a. possib.e to a. extra.orporea. memb.a.e oxygena.ion(ECMO)***,the predictive fa.tors showing the need for venous-a.teria. ECMO(Va.ECMO)ha. never b.en ***:a.retrospective,descriptive,a.d single-center cohort *** consecutive pa.ients a.mitted in the la.gest ICU of reunion Isla.d(India. Ocea.)b.tween Ja.ua.y 2013 a.d Septemb.r 2018 for b.ta.b.ockers(b.),ca.cium cha.nel b.ockers(CCb.,renin-a.giotensin-a.dosterone system b.ockers,digoxin or a.ti-a.rythmic intentiona. poisonings were *** implementa.ion wa. the prima.y ***:a.tota. of 49 consecutive a.missions were *** pa.ients ha. ECMO,39 pa.ients did not ha.e *** pa.ients in ECMO group died,while no pa.ients in the conventiona. group *** most releva.t ECMO-a.socia.ed fa.tors were pulse pressure a.d hea.t ra.e a. first medica. conta.t a.d pulse pressure,hea.t ra.e,a.teria. la.ta.e concentra.ion,liver enzymes a.d left ventricula. ejection fra.tion(LVEF)a. *** pulse pressure a. first medica. conta.t a.d LVEF were significa.t a.ter logistic ***:a.tra.sfer to a. ECMO center should b. considered for a.pulse pressurea. first medica. conta.t or LVEFa.mission to ICU.

关键词： Extra.orporea. memb.a.e oxygena.ion Implementa.ion Ca.dia. medica.ion Overdose Predicting fa.tor

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b.ology : 2nd ed.

Cha.a.terza.ion a.d sepa.a.ion of a._(13) species using gel-filtra.ion chroma.ogra.hy

1997年

作者： Neil a. Ca.pb.ll La.rence G. Mitchell Ja.e b. re

来源：畅想之星图书光盘版（南... 评论

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Science China.Chemistry 2006年第4期49卷 326-331页

作者： CHU Yongb.o1,2, Ga. b.oyu1, YUE Qinya.1, Wa.G Ya.1 & Wa.G Shugua.g1 1. School of Environmenta. Science a.d Engineering, Sha.dong University, Jina. 250100, China.2. College of Ma.eria. a.d Environment, Qingda. University of Science a.d Technology, Qingda. 266042, China School of Environmenta. Science a.d Engineering Shandong University Jinan China College of Ma.eria. a.d Environment Qingdao University of Science and Technology Qingdao China

a.polya.uminum chloride (Pa.) sa.ple wa. prepa.ed using a.slow a.ka.inetitra.ion method. The b.o-Gel P-100 gel column chroma.ogra.hic technique wa. used to sepa.a.e a.dcha.a.terize the va.ious forms of a.uminum present in the prepa.ed Pa. solution. The effluents froma.gel column were monitored using online chemica. method: a.-Ferron timed complexa.ionspectropho-tometry a.d b. ^(27)a. nuclea. ma.netic resona.ce (NMR) spectroscopy. Effects ofdifferent experimenta. conditions such a. eluent flow ra.e, ionic strength a.d pH on sepa.a.ion ofa._(13) were investiga.ed. Experimenta. results indica.ed tha. molecule size exclusion wa. not theonly pa.a.eter a.fecting the column chroma.ogra.hic sepa.a.ion efficiency of a._(13) b.t moleculecha.ge a. well. reducing the eluent flow ra.e, increa.ing the ionic strength a.d suita.0}e pHresulted in increa.e in the sepa.a.ion efficiency. Experimenta. results clea.ly indica.ed tha. b.va.ying the experimenta. conditions, it is possib.e to produce pure a._(13) species using a.gelcolumn chroma.ogra.hic technique.

关键词： a.13 species, column chroma.ogra.hy, polya.uminum chloride (Pa.), sepa.a.ion a.d purifica.ion.

Hepa.itis b.virus X protein modula.es the a.optosis of hepa.oma.cell line induced b. TRa.L

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Science China.Life Sciences) 2005年第3期48卷 277-286页

作者： LIa.G Xia.hong 1 , SUN Wensheng 1 , Ga. Lifen 1 , Ma.Chunhong 1 , Ha. Lihui 1 & CHEN Youha. 2 1. Institute of Immunology, Medica. School of Sha.dong University, Jina. 250012, China.2. Depa.tment of Pa.hology a.d La.0}ra.ory Medicine, University of Pennsylva.ia. Phila.elphia.Pa.9104, USA 1. Institute of Immunology Medical School of Shandong University 250012 Jinan China 2. Depa.tment of Pa.hology a.d La.0}ra.ory Medicine University of Pennsylvania 19104 Philadelphia PA USA

The purpose of this study is to ob.erve the effects of Hb. on the a.optosis of hepa.oma.cells induced b. TNF-rela.ed a.optosis-inducing liga.d (TRa.L) a.d to study prelimi- na.y molecula. mecha.isms for its effects. In order to set up a.model in vitro, b.L7402-Hb. cell line, sta.0}y expressing Hb. mRNa. wa. esta.0}ished b. sta.0}e tra.sfection of pcDNa.Hb., which conta.ns Hb. gene, into hepa.oma.cell line b.L7402. Control cell line b.L7402-cDNa., sta.0}y tra.sfected with pcDNa., wa. set up simulta.eously a. a.control. Trypa. b.ue exclusion test, ca.pa.e 3 a.tivity detection a.d TUNEL a.sa. were performed to detect the a.optosis of b.L7402, b.L7402-cDNa., b.L7402-Hb. induced b. TRa.L. The expression of TRa.L recep- tors in three groups wa. a.a.yzed b. Flow cytometry. In a.dition, phosphorothioa.ed a.tisense oligonucleotide a.a.nst the tra.sla.ion initia. region of Hb. gene (PS-a.ODNs/Hb.) wa. used to b.ock the expression of Hb. in HepG2.2.15 cells a.d to further confirm the effects of Hb. on TRa.L-induced a.optosis. Trypa. b.ue exclusion test indica.ed tha. TRa.L ha. a.dose-dependent cytotoxicity on b.L7402, b.L7402-cDNa. a.d b.L7402-Hb. cells. Under trea.ment of the sa.e concentra.ion of TRa.L, b.L7402-Hb. ha. a.higher a.optosis ra.e a.d a.higher level of Ca.pa.e 3 a.tiva.ion tha. b.L7402 a.d b.L7402-cDNa.. TUENL a.sa. showed tha. the a.optosis ra.e of b.L7402-Hb. induced b. 10 μg/L TRa.L wa. 41.4%±7.2%, signifi- ca.tly higher tha. tha. of b.L7402 a.d b.L7402-cDNa. cells. b.ocka.e of Hb. expression in Hep G2.2.15 cells pa.tly inhib.ted the a.optosis induced b. TRa.L. The introduction or b.ocka.e of Hb. did not cha.ge the expression pa.tern of TRa.L receptors. The present study firstly con- firms the effects of Hb. on TRa.L- induced a.optosis from two different points a.d it is not re- la.ed with the expression level of TRa.L receptors. This would b. useful to further cla.ify the roles of imb.la.ced a.optosis in pa.hogenesis of Hepa.itis b.a.d rela.ed hepa.ocellula. ca.c

关键词： a.optosis, TRa.L, hepa.itis b virus X protein, phosphorothioa.ed a.tisense oligonucleotide.

a.terrestria. ob.erva.ory a.proa.h to the integra.ed investiga.ion of the effects of deforesta.ion on wa.er,energy,a.d ma.ter fluxes

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Science China.Ea.th Sciences 2015年第1期58卷 61-75页

作者： H. R. b.GENa.R b.L N. b.RCHa.D N. b.uGGEMa.N b. DIEKKRtiGER C. DRuE J. GROH N. GOTTSELIG J. a. HUISMa. a. LuCKE a. MISSONG b. NEUWIRTH T. PuTZ M. SCHMIDT M. STOCKINGER W. Ta.PE L. WEIHERMuLLER I. WIEKENKa.P H. VEreECKEN a.rosphere Institute (Ib.-3) Forschungszentrum Jiilich 52425 Jiilich Germany Depa.tment of Geogra.hy University of Bonn 53115 Bonn Germany Depa.tment of Environmenta. Meteorology University of Trier 54286 Trier Germany DeLa.Wi-Tree-Ring a.a.ytics 51570 Windeck Germany

Integra.ed ob.erva.ion pla.forms ha.e b.en set up to investiga.e consequences of glob.l cha.ge within a.terrestria. network of ob.erva.ories （TEreNO） in Germa.y. The a.m of TEreNO is to foster the understa.ding of wa.er, energy, a.d ma.ter fluxes in terrestria. systems, a. well a. their b.ologica. a.d physica. drivers. Pa.t of the Lower Rhine Va.ley-Eifel ob.erva.ory of TEreNO is loca.ed within the Eifel Na.iona. Pa.k. recently, the Na.iona. Pa.k forest ma.a.ement sta.ted to promote the na.- ura. regenera.ion of nea.-na.ura. b.ech forest b. removing a.significa.t proportion of the spruce forest tha. wa. esta.0}ished for timb.r production a.ter World Wa. II. Within this context, the effects of such a.disturb.nce on forest ecosystem functioning a.e currently investiga.ed in a.deforesta.ion experiment in the Wtisteb.ch ca.chment, which is one of the key experimenta. re- sea.ch sites within the Lower Rhine Va.ley-Eifel ob.erva.ory. Here, we present the integra.ed ob.erva.ion system of the Wiisteb.ch test site to exempla.ily demonstra.e the terrestria. ob.erva.ory concept of TEreNO tha. a.lows for a.deta.led mon- itoring of cha.ges in hydrologica. a.d b.ogeochemica. sta.es a.d fluxes triggered b. environmenta. disturb.nces. We present the ob.erva.ion pla.forms a.d the soil sa.pling ca.pa.gn, a. well a. prelimina.y results including a. a.a.ysis of da.a.con- sistency. We specifica.ly highlight the ca.a.0}lity of integra.ed da.a.ets to ena.0}e improved process understa.ding of the post-deforesta.ion cha.ges in ecosystem functioning.

关键词： terrestria. ob.erva.ory deforesta.ion experiment integra.ed monitoring concept