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Heterologous expression,chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata

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Asian Pacific Journal of Tropical Medicine 2014年第2期7卷 85-92页

作者： w.wP.Rodrigo R.S Dassanayake E.H.Karunanayake Y.I.N.Silva Gunawardene O.VDS.J.weerasena Department of Chemistry Faculty of ScienceUniversity of Colombo90Cumaratunga Munidasa Mawatha.Colombo 03Sri Lanka Institute of biochemistry Molecular Biology and BiotechnologyUniversity of Colombo90Cumaratunga Munidasa MawathaColombo 03Sri Lanka Molecular Medicine Unit Faculty of MedicineUniversity of Kelaniya

Objective:To clone,express and purify a putative parasitic nematode specific protein of Setaria digitata(***),filarial nematode that infects livestock and cause significant economic losses in Far East and Asia to he used for structural and functional ***:To characterize uneharacterized gene of,***(SDUG),the herterologous expression of SDUG was carried out in the pET[cloned into pET45b(+)]expression system initially and co-expression of SDUC using chaperoiie plasmids pG-KJE8,pGro 7,pKJE7,pG-Tf2 and pTf16 containing chapcrone proteins of dnaK-dnaJ-grpE-groES-gro-E,groES-groEL,dnaK-dnaJ-grpE,groES-groEL-tig,and tig respectively,was carried out ***:Expression of SDUG was seen when Escherichia coli strain bI.21(DE3)is used,while concentrating protein largely into the insoluble *** co-expression of SDUG using chaperoiie plasmid mediated system indicated a significant increase of the protein in the soluble *** the chaperon plasniid sets,the highest amount of recombinant SDUP in the soluble fraction was seen when pGro7 was used in the presence of2 mg/mL L-arabinosc and 0.6M IPTG concentration in the culture medium and for 3 h of incubation at the temperature of 28℃.Recombinant SDUG was purified both from soluble and insoluble fractions using Ni affinity ***-PAGE and western blot analyses of these proteins revealed a single band having expected size of^24 ***:SDUG seems to be more aggregate-prone and hydrophobic in nature and such protein can make soluble by correct selecting the inducer concentrations and induction temperature and its duration.

关键词： Setaria digitata Uncharacterized gene Chaperon Co-expression

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Cyclosporine and Hepatitis C

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Open Journal of Organ Transplant Surgery 2012年第4期2卷 32-36页

作者： Ryan Caballes Mark w. Russo Department of Medicine Carolinas Medical Center Charlotte NC Transplant Center 3rd floor Annex bldg 1000 Blythe Blvd Carolinas Medical Center Charlotte NC

End stage liver disease from hepatitis C is a leading indication for liver transplantation. Recurrent hepatitis C after liver transplant may lead to cirrhosis and graft failure in up to 25% of recipients five years after liver transplantation. Anti-viral therapy is challenging after liver transplantation due to increased side effects including cytopenias and decreased efficacy compared to the nontransplant population. Tacrolimus and cyclosporine are the most common immunosuppressants used to prevent graft rejection. Tacrolimus is more potent than cyclosporine and may be preferred to cyclosporine. However, cyclosporine may have activity against hepatitis C and may have a theoretical advantage to tacrolimus in hepatitis liver transplant recipients. Cyclosporine may inhibit NS5b and NS5A protein complexes and increase endogenous interferon activity. Cyclophilin inhibitors without immunosuppressive properties are under development and represent a novel mechanisms for inhibiting HCV replication.

关键词： Immunosuppression Liver Transplant

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评估开发用于支持MR检查安全的便携磁警报传感器

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国际医学放射学杂志 2011年第6期 610-611页

作者： C. Martin T. Frauenrath C. zerdem w. Renz T. Niendorf 谢明伟 berlin Ultrahigh Field Facility (b.U.F.F.) Max-Delbrueck Center for Molecular MedicineRobert-Roessle-Strasse 1013125 Berlin Germany

目的本研究主要目的在于设计一个小型且可移动的磁警报传感器,详细评估其传感器激活与非激活态的一致性及其对磁场的敏感性,并展示其在1.5、3.0及7.0TMR边缘磁场环境中的应用。方法磁警报传感器由一系列叶状

关键词： MRI MR安全性磁警报传感器高场MRI 超高场MRI

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How Often will It Rain?

How Often Will It Rain?

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2008年海峡两岸气象科学技术研讨会

作者： Susan Solomon Robert w.Portmann NOAA Earth System Research Laboratory BoulderColorado

＜正＞Daily precipitation data from climate change simulations using the latest generation of coupled climate system models are analyzed for potential future changes in precipitation *** the emission scenarios SRES b1（a low projection）,A1b（a medium projection）,and A2（a high projection）during the 21st century,all the models consistently show a shift towards more intense and extreme precipitation for the globe as a whole and over various *** both SRES b1 and A2,most models show decreased daily precipitation frequency and all the models show increased daily precipitation intensity. The multi-model averaged percentage increase in the precipitation intensity（2.0%/ K）is larger than the magnitude of the precipitation frequency decrease（-0.7%/K）. However,for very heavy precipitation（＞50 mm/d）,the percentage increase in frequency is much larger than the increase in intensity（31.2%vs.2.4%）,so that there is a shift in the frequency distribution of precipitation towards increased *** climate model-projected increases in daily precipitation intensity are,however,smaller than that based on simple thermodynamics（～7%/K）.Multi-model ensemble means show that precipitation amount increases during the 21st century over high latitudes,as well as over currently wet regions in low-and mid-latitudes more than other *** increase mostly results from a combination of increased frequency and *** the dry regions in the subtropics,precipitation amount generally declines because of decreases in both frequency and *** indicates that wet regions may get wetter and dry regions may become drier mostly because of simultaneous increase（decrease）of precipitation frequency and intensity.

关键词： Climate change Precipitation Frequency Intensity

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水稻白叶枯病(Xanthom onas campestris ***)抗性遗传研究 Ⅲ.两个成株抗性基因X_a-6与X_a-3的等位性分析

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作物学报 1991年第3期17卷 233-237页

作者：章琦施爱农王春莲阙更生 T.w.Mew 中国农业科学院作物育种栽培所国际水稻所

研究了两个品种早生爱国3号和辛尼斯对菲律宾水稻白叶枯病菌4个生理小种、日本小种1和我国菌株的成株抗性遗传和抗性基因的等位关系。这两个抗病品种与感病品种沈农1033杂交所得的 F_1、F_2和 b_1F_1群体的成株抗性反应,表明它们均携有... 详细信息

研究了两个品种早生爱国3号和辛尼斯对菲律宾水稻白叶枯病菌4个生理小种、日本小种1和我国菌株的成株抗性遗传和抗性基因的等位关系。这两个抗病品种与感病品种沈农1033杂交所得的 F_1、F_2和 b_1F_1群体的成株抗性反应,表明它们均携有一对显性基因。将早生爱国3号同辛尼斯杂交进行直接等位性测定,并将邳早15和蚌珠芒分别与辛尼斯和早生爱国3号杂交进行间接等位测定,这5个组合的 F_2群体都没有分离出感病植株,表明这4个品种的成株抗性都是由同一基因控制的,并且验证了辛尼斯在 X_a-3位点上具有一等位基因。

关键词：水稻白叶枯病抗病性遗传

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流感嗜血杆菌性流感引起的眼内炎

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世界核心医学期刊文摘（眼科学分册） 2005年第3期1卷 53-54页

作者： Yoder D.M. Scott I.U. Flynn Jr. H.w. Miller D. 黎黎

To investigate the clinical settings, management strategies, antibiotic sensit ivities, and visual acuity outcomes for eyes with endophthalmitis caused by Haem ophilus influenzae. Retrospective, noncomparative, consecutive case series. The medical records were reviewed of all patients treated for culture proven H. inf luenzae endophthalmitis at a single institution between January 1, 1980, and Dec ember 31, 2002. Visual acuity and antibiotic sensitivities. The study included 1 6 eyes of 16 patients with a median age of 68 years (range, 6 months-83 years) and a median follow up of 26 months (range, 2 months-15 years). Clinical setti ngs included post trabeculectomy (n=7), postcataract surgery (n=6), post pars plana vitrectomy (n=1), post secondary intraocular lens insertion (n=1), and po stsuture removal from an extracapsular cataract wound (n=1). Eleven (69%) cases were of delayed onset ( > 6 weeks from surgery/event), with a median interval b etween surgery/event and presentation with endophthalmitis of 18 months (range, 44 days-21 years); 5 (31%) cases were of acute onset (median, 6 days; range, 2 -14 days). Presenting visual acuity was hand movements or better in 7 (44%) ey es. A vitreous tap and inject was performed initially in 9 (56%) eyes, and a vi trectomy was performed initially in the remaining 7 (44%) eyes. All eyes receiv ed intravitreal antibiotics on the day of presentation, and 11 (69%) received i ntravitreal dexamethasone. In vitro testing of the H. influenzae isolates reveal ed that 14 of 16 (88%) were sensitive to vancomycin, ampicillin, and trimethopr im/sulfa methoxazole; 15 of 16 (94%) were sensitive to aminoglycosides (1 isol a te was resistant to gentamicin); and all were sensitive to cephalosporins, fluor oquinolones, and carbapenems. The organisms were sensitive to at least 1 of the initial antibiotics administered in all cases. Final visual acuity was 5/200 or better in 6 (38%) eyes, and 6 (38%) eyes had a final visual acuity of no light pe

关键词：流感嗜血杆菌眼内炎玻璃体切除抗生素敏感性晶状体囊外摘除小梁切除手术玻璃体切割术睫状体平坦部晶体植入临床病史

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世界卫生组织人类微小病毒b19DNA核酸扩增技术国际标准化的首次建立

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国外医学（输血及血液学分册） 2003年第1期26卷 82-83页

作者： J. Saldanha N.Lehe M.w.Yu et al 刘敬闪尹志峰河北省血液中心河北省医药物资供销公司

人类微小病毒b19(b19)可引起传染性红斑(EI),溶血性贫血病人的暂时再障危机(TAC),并且与胎儿的死亡,关节炎以及慢性贫血有关。b19在人群中感染很普遍并且多数人群终生带有这种病毒(大约40～60％的成年人20岁以后有b19抗体)。用于制备血... 详细信息

人类微小病毒b19(b19)可引起传染性红斑(EI),溶血性贫血病人的暂时再障危机(TAC),并且与胎儿的死亡,关节炎以及慢性贫血有关。b19在人群中感染很普遍并且多数人群终生带有这种病毒(大约40～60％的成年人20岁以后有b19抗体)。用于制备血液产品的大部分血浆中含有b19。

关键词：世界卫生组织人类微小病毒 b19DNA 核酸扩增技术国际标准化

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Iatrogenic extrahepatic bile duct injury in 182 patients: causes and management

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Hepatobiliary & Pancreatic Diseases International 2002年第2期1卷 265-269页

作者： Yang F.-Q. Dai X.-w. wang L. Yu Y. Department of Hepatobiliary Surgery Second Hospital China Medical University Shenyang 110004 China

Objective: To describe the causes and treatment ofiatrogenic bile duct injury caused by ***: 182 patients with iatrogenic extrahepaticbile duct injury from 4 university hospitals of Chinawere reviewed. Details of primary cholecystectomy,biliary reconstruction as well as postoperative ma-nagement were recorded. All patients were followedup for at least 6 months (6 months to 9 years, medi-an 3.5 years). The adequacy of repair was assessedby regular evaluation of the patients clinical statusand liver function variables. Hepatobiliary b-ultra-sonography was used routinely in the follow up of pa-tients, and magnetic resonance cholangiopancreatog-raphy was applied in the patients suggestive of ***: In 152 patients, bile duct injury happenedduring open cholecystectomy, and in 30 patients dur-ing laparoscopic cholecystectomy. All the injuries de-veloped during anterograde cholecystectomy (at theCalot’s triangle). All the patients with these injuriesunderwent choledochocholedochostomy or Roux-en-Ycholedochojejunostomy with good results (161 pa-tients), recurrent stricture (11), and death (10).Conclusions: During cholecystectomy, the Calot’s tri-angle should be identified anatomically, but retro-grade cholecystectomy is the optimal choice. bileduct injury should be discovered as soon as possibleand be managed timely. Different operative methodsare optional according to the degree of injury and thepostoperative period.

关键词： iatrogenic injury bile duct laparoscopic cholecystectomy choledochojejunostomy magnetic resonance cholangiopancreatography biliary hepatic cirrhosis

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双色激光共振增强的气相C6H6分子的光电离谱

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量子电子学 1986年第4期 297-298页

作者：范俊颖 G.Müller J.L.Lyman w.E.Schmid K.L.Kompa 中国科学院上海光学精密机械研究所 Max-Planck-Institut fur Quautenoptik D-8046 Garching b.Munchen west Germany Los Alamos Natianal Laboratory New Mexico 87545 U.S.A. 中国科学院上海光学精密机械研究所

我们的实验研究了高于离化阈值约为3000cm-1的离化谱特性。实验证明离子的产额不但与中间电子能级1b2u的振动态有关,也依赖于离化激光的波长。从得到的离子谱的结构和强度我们可以得到离子的电子基态2E1g的振动频率及分子参数,可以达... 详细信息

我们的实验研究了高于离化阈值约为3000cm-1的离化谱特性。实验证明离子的产额不但与中间电子能级1b2u的振动态有关,也依赖于离化激光的波长。从得到的离子谱的结构和强度我们可以得到离子的电子基态2E1g的振动频率及分子参数,可以达到较高的精度。自离化Rydberg能级对离化截面的贡献非常显著。某些自离化共振峰可以确认为是这些Rrdberg

关键词：光电离 C6H6 离化 Rydberg 振动能级电子能级分子参数产额共振峰电子态

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Numerical simulation of tumor angiogenesis under the effect of Endostatin:considering mechanical environment in matrix and inhibiting effect of anti-angiogenic factor

Numerical simulation of tumor angiogenesis under the effect ...

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第九届全国生物力学学术会议

作者： Kalkabay Gulnar M.w.Collins Department of Mechanics and Engineering Science Fudan University brunel Institute for bioengineering Brunel University

To investigate tumor angiogenesis under the influence of Endostatin,mathematical modeling and numerical simulation of tumor angiogenesis are performed,with the mechanical environment in matrix,the inhibiting effects of Angiostatin and Endostatin into *** model assumes that the migration of endothelial cells is influenced mainly by four factors:random motility,convection induced by ECM traction,chemotaxis in response to tumor angiogenic factors

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