检索结果-南通市图书馆

Isolation, Cultivation, and Morphological Characteristics of Hair Follicle Adult Stem Cells in the bulge Region in Mouse and Human

引用

Microscopy Research 2020年第2期8卷 9-30页

作者： bélgica j. molina Héctor j. Finol Center for Electron Microscopy Science Faculty Central University of Venezuela Caracas Venezuela Dermatology Service University Hospital of Caracas Caracas Venezuela

Skin contains various populations of stem cells (SCs). Among these are hair follicle stem cells (HFSCs) in the bulge region. The behavior of HFSCs deserves to be widely studied due to the benefits to be derived from their identification, isolation, and amplification. Skin samples of newborn mice (n = 32) and human adults (n = 10) were used, and the bulge region was isolated and cultured. The isolation and characterization of cells were conducted through immunocytochemistry and immunofluorescence, using mainly CD34 and CD200 monoclonal antibodies. Initially, cells grew slowly from the explant around the bulge region, accruing cells with different morphology in both mouse and human, latter being mostly polygonal;the mouse cells reaching confluence faster (5 to 7 days) than the human (12 to 15 days). It was possible to isolate into subcultures cells with small size (10 - 13 μm diameter), round-shape, scant cytoplasm, central prominent nucleus and with nucleolus, which formed colonies, maintaining their phenotype in a high proportion (77% - 83% and 91% in mouse and human, respectively), without showing changes in their morphology during almost 7 months in the mouse cells, and a month and a half in the human. These results demonstrate that the selection, the isolation, and the conditioned mediums allowed population increases of bulge cells and indicate that cultured cells may retain their sternness in that they maintained their phenotypic characteristics, expressed specific markers for SCs, and showed a high proliferative capacity for long periods. Hair follicles, in mice and humans, are important repositories of multipotent stem cells, due to their tendency to differentiate into keratinocytes. Human HFSCs, obtained by depilation, preserve their potential for proliferation and prove to be easily accessible. This suggests that the bulge cells may present an alternative source of autologous stem cells for tissue engineering and regenerative medicine.

关键词： Stem Cells Hair Follicle bulge Niche Alopecia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification of bulge Stem Cells in Mouse and Human Hair Follicles

引用

Microscopy Research 2018年第3期6卷 19-29页

作者： bélgica j. molina Elda Giansante Héctor j. Finol Center of Electron Microscopy Science Faculty Central University of Venezuela Caracas Venezuela Dermatology Service University Hospital of Caracas Caracas Venezuela

The skin contains various populaions of stem cells, but its characterization has been hampered by lack of markers and unclear location. The hair follicle has a niche for stem cells called a “bulge” which acts as a reservoir of multipotent stem cells. In the study reported here, an immunohistochemical and immunofluorescence analysis was performed on mouse and human tissues in order to determine the possible presence of stem cells of hair follicle through cytokeratin 15 (CK15), CD34, and CD200 markers identified as crucial to the stem cells and to identify the bulge region. Mouse (n = 7) and human (n = 7) skin samples were used. The expression of proteins was determined by the indirect immunoperoxidase technique and a secondary antibody bound to a fluorochrome. The specificity of staining was evaluated by negative controls. The results revealed that the stem cells associated with CD34 and CD200 antibodies were differentially expressed in the interfollicular epidermis, sebaceous glands, and bulge region, indicating that, in mice, CD34 and, in humans, CD200 are more specific than CK15 in detecting bulge cells. It also suggests that CD34 is specific for mouse bulge cells, while CD200 might have specificity for progenitor cells and partially differentiated cells in humans.

关键词： Stem Cells Hair Follicle bulge CD34 CD200

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Physical activity and amyloid beta in middle-aged and older adults:A systematic review and meta-analysis

引用

j.urnal of Sport and Health Science 2024年第2期13卷 133-144页

作者： María Rodriguez-Ayllon Patricio Solis-Urra Cristina Arroyo-ávila Miriam álvarez-Ortega Pablo molina-García Cristina molina-Hidalgo Manuel Gómez-Río belinda brown Kirk I.Erickson Irene Esteban-Cornej. Department of Epidemiology Erasmus University Medical Center RotterdamRotterdamGD 3015the Netherlands Department of Physical Education and Sports Faculty of Sport SciencesSport and Health University Research Institute(iMUDS)University of GranadaGranada 18071Spain Faculty of Education and Social Sciences Universidad Andres BelloVina del Mar 2531015Chile Department of Nuclear Medicine Virgen de las Nieves University HospitalInstitute of Biosanitary Research of Granada(IBS)Granada 18014Spain Physical Medicine and Rehabilitation Service Virgen de las Nieves University HospitalInstitute of Biosanitary Research of Granada(IBS)Granada 18014Spain Department of Psychology University of PittsburghPittsburghPA 15260USA Centre for Healthy Ageing Health Futures InstituteMurdoch UniversityMurdochWA 6150Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia Advent Health Research Institute Neuroscience Institute OrlandoOrlandoFL 32803USA Physiopathology of Obesity and Nutrition Research Center(CIbERobn) Institute of Health Carlos Ⅲ(ISCⅢ)Madrid 28029Spain

background:One of the pathological hallmarks distinguishing Alzheimer’s disease from other dementias is the accumulation of amyloid beta(Aβ).Higher physical activity is associated with decreased dementia risk,and one potential path could be through Aβlevels *** aimed to explore the relationship between physical activity and Aβin middle-aged and older ***:A systematic search of PubMed,Web of Science,PsycINFO,Cochrane Central Register of Controlled Trials,and SPORTDiscus was performed from inception to April 28,*** were eligible if they included physical activity and Aβdata in adults aged 45 years or ***-level metaanalyses of intervention and observational studies were performed to examine the role of physical activity in modulating Aβ***:In total,37 articles were included(8 randomized controlled trials,3 non-randomized controlled trials,4 prospective longitudinal studies,and 22 cross-sectional studies).The overall effect size of physical activity interventions on changes in blood Aβwas medium(pooled standardized mean difference=-0.69,95%confidence interval(95%CI):-1.41 to 0.03;I^(2)=74.6%).However,these results were not statistically significant,and there were not enough studies to explore the effects of physical activity on cerebrospinal fluid(CSF)and brain Aβ.Data from observational studies were examined based on measurements of Aβin the brain using positron emission tomography scans,CSF,and *** physical activity was positively associated with Aβonly in the CSF(Estimate r=0.12;95%CI:0.05-0.18;I^(2)=38.00%).Conclusion:Physical activity might moderately reduce blood Aβin middle-aged and older ***,results were only near statistical significance and might be interpreted with caution given the methodological limitations observed in some of the included *** observational studies,higher levels of physical activity were positively associated with Aβonly in ***,further research is needed t

关键词： Aging Cognitive impairment Dementia Exercise PET

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plant cell wall-mediated disease resistance:Current understanding and future perspectives

引用

Molecular Plant 2024年第5期17卷 699-724页

作者： Antonio molina Lucıa j.rda Miguel Angel Torres Marina Martın-Dacal Diego j.se berlanga Patricia Fernandez-Calvo Elena Gomez-Rubio Sonsoles Martın-Santamarıa Centro de biotecnología y Genómica de Plantas Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA/CSIC)Campus de Montegancedo UPMPozuelo de Alarcon(Madrid)Spain Departamento de biotecnologıa-biología Vegetal Escuela Técnica Superior de Ingenierıa AgronomicaAlimentarıa y de BiosistemasUPMMadridSpain Centro de Investigaciones biologicas Margarita Salas Consejo Superior de Investigaciones Científicas(CSIC)Ramiro de Maeztu 928040 MadridSpain Misión biológica de Galicia Consejo Superior de Investigaciones Científicas(CSIC)Campus de Montegancedo UPMPozuelo de Alarcón(Madrid)Spain

beyond their function as structural barriers,plant cell walls are essential elements for the adaptation of plants to environmental *** walls are dynamic structures whose composition and integrity can be altered in response to environmental challenges and developmental *** wall changes are perceived by plant sensors/receptors to trigger adaptative responses during development and upon stress *** cell wall damage caused by pathogen infection,wounding,or other stresses leads to the release of wall molecules,such as carbohydrates(glycans),that function as damage-associated molecular patterns(DAMPs).DAMPs are perceived by the extracellular ectodomains(ECDs)of pattern recognition receptors(PRRs)to activate pattern-triggered immunity(PTI)and disease ***,glycans released from the walls and extracellular layers of microorganisms interacting with plants are recognized as microbe-associated molecular patterns(MAMPs)by specific ECD-PRRs triggering PTI *** number of oligosaccharides DAMPs/MAMPs identified that are perceived by plants has increased in recent ***,the structural mechanisms underlying glycan recognition by plant PRRs remain ***,this knowledge is mainly focused on receptors of the LysM-PRR family,which are involved in the perception of various molecules,such as chitooligosaccharides from fungi and lipo-chitooligosaccharides(i.e.,Nod/MYC factors from bacteria and mycorrhiza,respectively)that trigger differential physiological ***,additional families of plant PRRs have recently been implicated in oligosaccharide/polysaccharide *** include receptor kinases(RKs)with leucine-rich repeat and Malectin domains in their ECDs(LRR-MAL RKs),Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE group(CrRLK1L)with Malectin-like domains in their ECDs,as well as wall-associated kinases,lectin-RKs,and *** characterization of structural basis of glycans recogniti

关键词： Arabidopsis thaliana cell wall damage-associated molecular patterns DAMPs disease resistance glycans oligosaccharides pattern-triggered immunity PTl pattern recognition receptor PRRs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ON THE IDENTIFICATION OF RESIDUAL IMPACT PROPERTIES OF MATERIALS bY ACOUSTO-ULTRASONICS——A PATTERN RECOGNITION APPROACH

引用

Acta Mechanica Sinica 1995年第1期11卷 34-43页

作者： G.j.molina Y.M.Haddad Dept.of Mechanical Engineering University of OttawaOttawaCanada K1N 6N5

This paper deals with the application of Acousto-ultrasonics,in con- j.nction with Pattern Recognition and Classification techniques,to the identification of residual impact properties of a class of polymeric material,namely,Polyvinylchlo- ride(PVC).PVC specimens of different low-energy repeated impact damage states are processed by Acousto-ultrasonics(AU)to retrieve AU signals in the form of dig- italized *** AU signals are grouped as distinct classes,each pertaining to a known level of repeated impact *** features of these AU signals are used to build Pattern Recognition(PR)*** classifiers are used to identify unknown damage states in other PVC specimens by classifying the re- trieved AU signals as belonging to one of the *** obtained results indicate that Acousto-ultrasonics in combination with Pattern Recognition and Classification techniques can be used for the quantitative non-destructive identification of damage states in PVC specimens of unknown low-energy repeated impact conditions.

关键词： pattern recognition acousto-ultrasonics impact damage quantitative non-destructive examination

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fully integrated and broadband Si-rich silicon nitride wavelength converter based on bragg scattering intermodal four-wave mixing

引用

Photonics Research 2024年第3期12卷 I0001-I0010页

作者： VALERIO VITALI THALÍA DOMÍNGUEZ bUCIO HAO LIU j.SÉMANUEL LUQUE GONZÁLEZ FRANCISCO j.RADO-ROMERO ALEj.NDRO ORTEGA-MOÑUX GLENN CHURCHILL j.MES C.GATES j.MES HILLIER NIKOLAOS KALFAGIANNIS DANIELE MELATI j.NS H.SCHMID ILARIA CRISTIANI PAVEL CHEbEN j.GONZALO WANGÜEMERT-PÉREZ ÑIGO molina-FERNÁNDEZ FREDERIC GARDES COSIMO LACAVA PERIKLIS PETROPOULOS Optoelectronics Research Centre University of SouthamptonSouthampton SO171BJUK Electrical Computer and Biomedical Engineering DepartmentUniversity of PaviaPavia 27100Italy Telecommunication Research Institute(TELMA) Universidad de MálagaCEI Andalucı´a TECHE.T.S.I.Telecomunicación29010 MálagaSpain School of Science and Technology Nottingham Trent UniversityNottingham NG118NSUK Department of Electrical Engineering Eindhoven University of TechnologyEindhoven 5600 MBThe Netherlands Department of Materials Science and Engineering University of IoanninaIoannina 45110Greece Centre de Nanosciences et de Nanotechnologies UniversitéParis-SaclayCNRS91120 PalaiseauFrance Advanced Electronics and Photonics Research Center National Research Council CanadaOttawaOntario K1A 0R6Canada

Intermodal four-wave mixing(FWM)processes have recently attracted significant interest for all-optical signal processing applications thanks to the possibility to control the propagation properties of waves exciting distinct spatial modes of the same *** allows,in principle,to place signals in different spectral regions and satisfy the phase matching condition over considerably larger bandwidths compared to intramodal ***,the demonstrations reported so far have shown a limited bandwidth and suffered from the lack of on-chip components designed for broadband manipulation of different *** demonstrate here a silicon-rich silicon nitride wavelength converter based on bragg scattering intermodal FWM,which integrates mode conversion,multiplexing and de-multiplexing functionalities *** system enables wavelength conversion between pump waves and a signal located in different telecommunication bands(separated by 60 nm)with a 3 db bandwidth exceeding 70 nm,which represents,to our knowledge,the widest bandwidth ever achieved in an intermodal FWM-based system.

关键词： scattering pump modal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

超高分辨光子计数CT评估冠状动脉支架通畅性：与有创法冠状动脉造影的对照研究

引用

国际医学放射学杂志 2024年第5期 619-620页

作者： M.T.Hagar M.Soschynski R.Saffar M.F.molina-Fuentes j.Weiss A.Rau 苗彦许振华

目的与有创冠状动脉造影（ICA）对照，评价超高分辨光子计数CT血管成像（UHR PCD-CTA）检测冠状动脉支架通畅性的准确度。方法前瞻性纳入2022年8月—2023

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Involvement of sweet pepper Ca LOX2 in j.smonate-dependent induced defence against Western flower thrips

引用

j.urnal of Integrative Plant biology 2019年第10期61卷 1085-1098页

作者： Sandeep j.Sarde Klaas bouwmeester j.on Venegas-molina Anj. David Wilhelm boland Marcel Dicke Laboratory of Entomology Wageningen University P.O. Box 16 6700 AA Wageningen The Netherlands Laboratory of Phytopathology Wageningen University P.O. Box 16 6700 AA Wageningen The Netherlands Department of bioorganic Chemistry Max Planck Institute for Chemical Ecology Hans-Knoll-Straβe 8 D-07745 Jena Germany

Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell-content-feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in the defense of pepper plants against Western flower thrips (Frankliniella occidentalis). This was done through a combination of in-silico, transcrip-tional, behavioral *** datashowthat CaLOX2 is involved in j.smonic acid (j.) biosynthesis and mediates plant resistance. Expression of the j.-related marker genes, CaLOX2 and CaPIN II, was induced by thrips feeding. Silencing of CaLOX2 in pepper plants through virus-induced gene silencing (VIGS) resulted in low levels of CaLOX2 transcripts, as well as significant reduction in the accumulation of j., and its derivatives, upon thrips feeding compared to control plants. CaLOX2-silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that CaLOX2 mediates j.-dependent signaling, resulting in defenseagainstthrips. Furthermore,exogenous application of j. to pepper plants increased plant resistance to thrips, constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis.

关键词： Western flower thrips sweet pepper CaLOX2 j.smonate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Secondary male hypogonadism： a prevalent but overlooked comorbidity of obesity

引用

Asian j.urnal of Andrology 2018年第6期20卷 531-538页

作者： Maria molina-Vega Araceli Mufioz-Garach Miguel Damas-Fuentes j.sO Carlos Fernfindez-Garcia Francisco j.inahones Department of Endocrinology and Nutrition Virgen de la Victoria Universitary HospitalMalaga 29010Spain Institute of biomedical Research in Malaga（IbIMA） Virgen de la Victoria Universitary HospitalMalaga 29010Spain CIbER Pathophysiology of Obesity and Nutrition（Cb06/003） Institute of Health Carlos III（ISClII）Madrid 28029Spain.

Male hypogonadism associated with obesity is a very prevalent condition and is increasing in parallel with the epidemic prevalence of obesity. Low testosterone levels promote higher fat mass with reduced lean mass. Male hypogonadism is related to an increase in associated cardiometabolic complications, such as hypertension, type 2 diabetes mellitus, the metabolic syndrome, and cardiovascular disease. Its influence as a comorbidity of obesity is becoming more evident and should be evaluated and treated in at-risk patients. Mechanisms involved in this relationship include body composition changes, the presence of adipokines, insulin resistance, and other factors, some of which are still unknown. Weight loss and treatment to replace testosterone levels improve the metabolic profile and quality of life in patients with obesity and hypogonadism; these beneficial effects depend on treatment modality and duration of therapy. The use of testosterone replacement therapy may be indicated, as it has not been shown to increase cardiovascular risk, and retrospective studies suggest a reduction in events in men with metabolic syndrome and type 2 diabetes.

关键词： adipose tissue male hypogonadism obesity testosterone

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Selection of Maize Genotypes with Tolerance to Osmotic Stress Associated with Salinity

引用

Agricultural Sciences 2016年第2期7卷 82-92页

作者： Mónica b. Collado Mónica b. Aulicino Miguel j. Arturi María del C. molina Instituto Fitotécnico de Santa Catalina Facultad de Ciencias Agrarias y Forestales Universidad Nacional de La Plata Provincia de Buenos Aires Argentina CONICET

Thirteen different inbred lines in relation to the type of grain and life cycles were characterized by testing for osmotic stress associated with salinity. The identification of tolerant genotypes would be an effective strategy to overcome the saline stress. Osmotic stress reduces immediately the expansion of the roots and young leaves which determine a reduction in the size of the plant. A completely randomized design was adopted to test seedlings under controlled conditions of light and temperature. Two treatments were used: 0 mM NaCl (as control) and 100 mM NaCl. After 15 days of complete salinization, the seedlings were harvested and several morphological traits were measured. The morphological traits of growth were leaf growth (Ar1, Ar2, Ar3 and Ar4), dry masses of shoot and root (SDM and RDM, respectively). Also, traits associated with water economy were registered: leaf water loss (LWL) and relative water content (RWC). The morphological traits were expressed in relative terms, while the traits associated with the economy of water were expressed in absolute terms. Uni and multivariate techniques were applied to identify genotypes with divergent behaviors to osmotic stress tolerance. Also, a Tolerance Index was employed to identify superior genotypes. Four clusters were obtained after applying a Cluster Analysis and Principal Component Analysis (PCA). The genotypes were compared to each other with a test of DMS. The results obtained with different statistical techniques converged. Some variables presented a differential weight classification of genotypes. The morphological traits like RDM, SDM, Ar3, Ar4 and Ar5 were the most discriminating. Tolerance Index allowed to classify genotypes, thus SC2 and AD3 lines were that reached highest value of the index and therefore would be tolerant lines, while AF3 and LP3 had a low index and were seen as sensible.

关键词： Maize Salinity Osmotic Stress Tolerance Selection Index

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：