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Protective effect of <i>Camellia sinensis</i>on Methotrexate-Induced Small Intestinal Mucositis in Mice

Food and Nutrition Sciences 2014年第5期5卷 443-448页

作者： Samuel brito de Almeida Manuel Carlos Serra Azul Monteiro Ana Valêska Pinto de Lima Dalgimar beserra de Menezes Sandra Maria Nunes Monteiro Center of endocrinology Metabolism and Nutrition Studies of Brazilian Northeast Medicine Faculty/Federal University of Ceará Fortaleza Brazil department of Nutrition/Health Sciences Center/Federal University of Rio Grande do Norte Natal Brazil. department of Pathology and Forensic Medicine/ Medicine Faculty/Federal University of Ceará Fortaleza Brazil department of Physiology and Pharmacology/Medicine Faculty/Federal University of Ceará Fortaleza Brazil Medicine/Health Sciences Center University of Fortaleza Fortaleza Brazil

background: Green tea has been used as a daily beverage for several years. Anti-inflammatory effect of tea has also been depicted in different papers. Therefore we had set forward this study to examine the potential anti- inflammatory activity of green tea in small intestine mucositis experimental models. Aims: evaluation of anti- inflammatory effects of green tea on mice. Materials and Methods: Green tea decoction (20%) was prepared by soaking 20 g of green tea in 100 ml boiled water separately, soaked for 2 minutes and thereafter filtered. In- flammatory activity was induced using methotrexate (2.75 g/kg/24 h sc), and a protecting effect of mucositis con- dition was investigated by vitamin e and Camellia sinensis decoction. Study design: An experimental study was approved by an Animal ethical Commitee. Results: Green tea decoction (20%) has shown significant anti-in- flammatory effects (65% and 70%) on methotrexate-induced acute mucositis model. In villous atrophy Green tea decoction (10% and 20%) has shown no protecting action at different intestinal segments. but at intestinal crypt hyperplasia, green tea decoction has shown 65.74%, as compared to mucositis group. An increase of apoptotic bodies were acchieved at MTX group, CS reduced this occurrence. Conclusion: Taken together, our data indi- cate that green tea (20%) has a potential anti-inflammatory compared vitamin e antioxidant action and cor- roborates with the current trend of tea being promoted as “health drink”. However more pharmacological and biochemical assays is necessary to elucidate mechanisms.

关键词： Mucositis Vitamin e Camellia Sinensis Morphometry Mice Cathechins

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Nutritional and Mineral Variability in 52 Accessions of Common bean Varieties(Phaseolus vulgaris L.)from Madeira Island

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Agricultural Sciences 2014年第4期5卷 317-329页

作者： Carla S.S.Gouveia Gregório Freitas José H.de brito Jan J.Slaski Miguel A.A.Pinheiro de Carvalho banco de Germoplasma ISOPlexis Universidade da MadeiraFunchalPortugal Servico de Análise de Solos e Plantas Direccao Regional de Agricultura e Desenvolvimento RuralCamachaPortugal bioresource Technologies Alberta Innovates-Technology FuturesEdmontonCanada

The Phaseolus vulgaris L. is one of the traditional and most important leguminous crops in the Island of Madeira. The island’s bioclimatic tiers, agro-ecological environment and traditional farming practices had a great influence on the evolution of regional bean landraces. The variability of the nutritional and mineral seeds composition of 59 accessions of the Madeiran landraces, standard and commercial varieties was evaluated. Wide ranges of variability in biochemical parameters were reported among the Madeiran landraces, being the best sources of protein and mineral nutrition, according to the statistical results and literature comparative evaluation. Specifically, the content (g per 100 g DW) of ash ranged from 3.64-5.67, lipids from 0.57-2.86, protein from 18.55-29.69, starch from 23.40-52.65, soluble sugars from 2.97-6.84, while content of dry matter was from 83.35-93.55. The seeds also contained (per 100 g DW) between 2.55-4.83 g N, 0.30-7.50 g P, 1.30-2.49 g K, 0.10-0.18 g Mg, 4.10-10.00 mg Fe, 50.0-1.40 mg Cu, 2.20-5.00 mg Zn, 0.90-3.80 μg Mn and 0.20-2.40 μg b. This variability implies that the screened germplasm could serve as a source for breeding new varieties with improved biochemical and nutritional traits or could be highly recommended to meet specific dietary requirements. The cultivar Vaginha Grossa (ISOP 713) revealed low carbohydrate content that could be a good food choice for diabetics, while cultivar Vermelho (ISOP 724) bean should be offered as a valuable alternative source of protein and minerals in the local diet.

关键词： Phaseolus vulgaris L. beans Genetic Resources Proximal Analysis Nutritional Value Mineral Composition Variability Food Quality

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Germination of encholirium spectabile *** Schult.&*** in Response to Temperature and Water Stress

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American Journal of Plant Sciences 2014年第15期5卷 2237-2246页

作者： Marlene Feliciano Figueiredo Francisco Carlos barboza Nogueira Charles Lobo Pinheiro Selma Freire de brito Sebastiao Medeiros Filho University of Vale do Acarau—UVA Campus BetaniaCity of SobralBrazil Seed Analysis Laboratory Department of PhytotecnicFederal University of Ceara—UFCCampus do PiciCity of FortalezaBrazil

A study was conducted to examine the effects of different temperatures and water stress on encholirium spectabile seeds. We evaluated the germination percentage, speed and time of germination of small (4.01 mm), at temperatures of 15°C, 20°C, 25°C, 30°C, 35°C, 40°C and 45°C and a photoperiod of 12 hours of light. Seeds were subjected to osmotic potentials of 0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2 and -1.4 MPa, induced by solutions of polyethylene glycol. The results indicated an excellent germination of medium and large seeds at the temperatures of 20°C, 25°C, 30°C and 35°C. The temperature of 35°C provided the best mean germination time of large seeds and a higher speed rate of medium and large seeds. e. spectabile seeds germinate in a wide range of water deficit of -0.2 to -1.2 MPa. Germination reduced at concentrations of -1.0 and -1.2 MPa. No germination occurred at -1.4 MPa. Larger seeds showed the higher germination potential than medium and small seeds at the temperatures of 25°C, 30°C and 35°C and in the range ofwater deficit of -0.2 to -1.2 Mpa.

关键词： Seeds Size Native Species Osmotic Potential Polyethylene Glycol Semiarid Region

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An egg-shell bifunctional CeO_(2)-modified NiPd/Al_(2) O_(3) catalyst for petrochemical processes involving selective hydrogenation and hydroisomerization

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Journal of Rare earths 2021年第11期39卷 1382-1388,I0003页

作者： Franklin J.Mendez Javier A.Alves Yahse Rojas-Challa Oscar Corona Yanet Villasana Julia Guerra German Garcia-Colli Osvaldo M.Martinez Joaquin L.brito Centro de Quimica Instituto Venezolano de Invesdgaciones CientfficasCarretera PanamericanaKm 11 Altos de PipeCaracas1020AVenezuela departamento de estado Solido Instituto de FisicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaCiudad de Mexico04510Mexico departamento de Ingenieria Quimica Facultad de Ingenieria(UNLP)and Centro de Investigacion y Desarrollo en Ciencias Aplicadas"Dr.J.J.Ronco"(CINDECA)CCT-La Plata-CONICET-UNLPLa Plata1900Argentina departamento de Quimica Analitica Facultad de QufmicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaCiudad de Mexico04510Mexico Instituto Tecnoldgico Venezolano del Petroleo SA. Petrole os de Venezuela SA.Urb.Santa Rosa-Sector El TamborLos Teques1201Venezuela Instituto Superior de Formacion Docente Salome Urena Esquina Leonardo D'VinciSanto Domingo10114Republica Dominicana biomass to Resources Group Universidad Regional Amazonica IkiamKm 7 via MuyunaTena150150Ecuador departamento de Termodinamica y Fenomenos de Transferencia Universidad Simon BolivarValle de SartenejasCaracas1086Venezuela

The catalytic performance during the 1-butyne hydrogenation using two reduced Al_(2) O_(3)-supported Pdbased catalysts was carried out in a total recirculation system with an external fixed-bed *** lab-prepared egg-shell NiPd/CeO_(2)-Al_(2) O_(3) catalyst(NiPdCe) with Pd loading=0.5 wt%,Ni/Pd atomic ratio=1 and CeO_(2) loading=3 wt% was synthesized and characterized,and it was compared with an egg-shell Al_(2) O_(3)-supported Pd based commercial catalyst(PdCC).The reduced catalysts were characterized by X-ray diffraction,X-ray photoelectron spectroscopy,and high-resolution transmission electron *** textural characteristics and ammonia temperature-programmed desorption profiles of the fresh(unreduced) catalysts were also *** catalysts show high 1-butyne conversion and selectivity to 1-butene,but the catalysts also present important differences between hydroisomerizing and hydrogenating *** catalyst shows higher capability for hydroisomerization reactions,while the PdCC catalyst exhibits higher hydrogenating *** observed catalytic performances can be interesting for some industrial processes and can provide a guideline for the development of a Pd-based catalyst with specific catalytic properties.

关键词： 1-butyne bifunctional catalyst egg-shell Hydrogenation Hydroisomerization NiPd catalyst

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Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2006年第6期7卷 429-435页

作者： AbReU P.R.C. ALMeIDA M.C. beRNARDO R.M. beRNARDO L.C. brito L.C. GARCIA e.A.C. FONSeCA A.S. beRNARDO-FILHO M. department of biophysics and biometry Institute of Biology Roberto Alcantara Gomes University of Estate of Rio de Janeiro Rio de Janeiro 20551030 Brazil department of Physiology Biological Sciences and Health Center Federal University of Sergipe So Cristóvo Sergipe 49100000 Brazil department of Pharmacology and Psychobiology Institute of Biology Roberto Alcantara Gomes University of Estate of Rio de Janeiro Rio de Janeiro 20551030 Brazil

Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory dis-turbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (bC) are labelled with techne-tium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of bC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of bC with 99mTc. blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in bC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of bC with 99mTc. The data showed significant (Peration of ATI in bC from blood incubated with guava extract.

关键词：抗氧化剂锝放射性同位素番石榴汁血液要素

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Co-Inoculation of Soybean with bradyrhizobium and Azospirillum Promotes early Nodulation

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American Journal of Plant Sciences 2015年第10期6卷 1641-1649页

作者： Amaral Machaculeha Chibeba Maria de Fátima Guimaraes Osmar Rodrigues brito Marco Antonio Nogueira Ricardo Silva Araujo Mariangela Hungria department of Agronomy Universidade Estadual de Londrina(UEL)LondrinaBrazil embrapa Soja LondrinaBrazil Total biotecnologia Indústria e Comércio Ltda CuritibaBrazil

Soybean inoculation with elite strains of bradyrhizobium to improve nodulation, N2 fixation, and grain yield is well established worldwide. However, when grown in soils where N is deficient, soybean undergoes an initial phase of N starvation that may last up to 20 days after seedling germination due to the lack of synchronism between the phase when seed N reserves are exhausted and the moment when plants begin to benefit from the nitrogen fixed by the bacteria. Practices that promote early nodulation may play a key role in reducing the N starvation period. Azospirillum is a plant growth promoting rhizobacteria (PGPR) that can stimulate root hair formation and root growth, creating more sites for early root infection and nodule formation by N2-fixing bradyrhizobium spp. In this study, the effects of co-inoculating soybeans with bradyrhizobium spp. and Azospirillum brasilense on nodulation precocity and N2 fixation were evaluated under greenhouse and field conditions. Nodule number and dry weight, as well as plant and root dry weight and N accumulated in shoots at 15, 18, 21, 24 and 30 days after emergence (DAe) were evaluated in response to inoculation with bradyrhizobium spp. alone or when co-inoculated with Azospirillum sp. In the greenhouse, co-inoculated plants nodulated precociously as indicated by a significant increase (p Azospirillum helps plants to overcome environmental stresses.

关键词： biological Nitrogen Fixation Inoculation Soybean Symbiosis

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eXAFS study of cation reordering in NaYF_4:Yb^(3+),Tb^(3+) up-conversion luminescence materials

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Journal of Rare earths 2014年第3期32卷 226-229页

作者： H.F.brito J.Hls T.Laamanen T.Laihinen M.Lastusaari L.Pihlgren L.C.V.Rodrigues T.Soukka Instituto de Química Universidade de S?o Paulo department of Chemistry University of Turku Turku University Centre for Materials and Surfaces (MatSurf) department of Chemistry University of Turku FI-20014 Turku Finland Turku University Centre for Materials and Surfaces (MatSurf) Turku Finland University of Turku Graduate School (UTUGS) Doctoral Programme in Physical and Chemical Sciences Graduate School of Materials Research (GSMR) department of biochemistry University of Turku

The NaYF4：yb3＋,Tb3＋（Xyb： 0.20, XTb： 0.04） materials were prepared using the co-precipitation method, lne as-preparea material was washed either with or without water in addition to ethanol and thereafter annealed for 5 h at 500℃. This resulted in materials with moderate or very high up-conversion luminescence intensity, respectively. The structural study carried out with X-ray powder diffraction revealed microstrains in the rare earth （R） sublattice that were relaxed for the material with very high up-conversion intensity thus decreasing energy losses. The local structural details were investigated with R LⅢ and Y K edge ex- tended X-ray absorption fine structure （eXAFS） using synchrotron radiation. Around 10 tool.% of the Yb3＋ ions were found to occupy the Na site in the material with very high up-conversion intensity. These Yb species formed clusters with the Tb3＋ ions occupying the regular Na/R sites. Such clustering enhanced the energy transfer between Yb3＋ and Tb3＋ thus intensifying the up-conversion emission.

关键词： eXAFS up-conversion ytterbium terbium cation reordering

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Production of Manganese Peroxidase by Trametes villosa on Unexpensive Substrate and Its Application in the Removal of Lignin from Agricultural Wastes

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Advances in bioscience and biotechnology 2014年第14期5卷 1067-1077页

作者： Marilia Lordelo Cardoso Silva Volnei brito de Souza Veronica da Silva Santos Helio Mitoshi Kamida Joao Ronaldo T.de Vasconcellos-Neto Aristoteles Goes-Neto Maria Gabriela bello Koblitz Technology department State University of Feira de Santana(UEFS)Feira de SantanaBrasil biology department State University of Feira de Santana(UEFS)Feira de SantanaBrasil Food Technology department Federal University of the State of Rio de Janeiro(UNIRIO)Rio de JaneiroBrasil

Manganese peroxidase (MnP) is a ligninolytic enzyme that is involved in the removal of lignin from the cell wall of plants. This removal facilitates the access of hydrolytic enzymes to the carbohydrate polymers that are hydrolyzed to simple sugars, which allows the subsequent fermentation to obtain bioproducts, such as ethanol. In this work, response surface methodology (RSM) was employed to optimize the culture conditions on unexpensive substrate for MnP secretion by Trametes villosa. Three independent variables were evaluated (i.e., temperature, moisture content and pH). The crude extract containing MnP was used in the delignification experiment and it caused a reduction in lignin content for all residues tested: 35.05 ± 1.45 (%) for the sugar cane bagasse;63.11 ± 0.06 (%) for the sisal fiber and 39.61 ± 0.39 (%) for the coconut shell, under the reaction conditions tested after 4 hours of fermentation. The preliminary results exhibited the potential application of this enzyme in the removal of lignin from plant residues. However, the conditions should be evaluated and optimized for each residue type.

关键词： Ligninolytic enzymes basidiomycete Sugarcane bagasse Coconut Shell Sisal Fiber

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Antitumor effects of electrochemical treatment

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Chinese Journal of Cancer Research 2013年第2期25卷 223-234页

作者： Héctor Manuel Camué Ciria Maraelys Morales González Lisset Ortíz Zamora Luis enrique bergues Cabrales Gustavo Victoriano Sierra González Luciana Oliveira de Oliveira Rodrigo Zanella Antonio Carlos buzaid Orlando Parise Luciana Macedo brito Cesar Augusto Antunes Teixeira Marina das Neves Gomes Gleyce Moreno Venicio Feo da Veiga Marcos Telló Carla Holandino Research department National Centre of Applied Electromagnetism Oriente University Pharmacy department Oriente University Finlay Institute La lisa. La Habana Cuba Veterinary Teaching Hospital Federal University of Rio Grande do Sul. Porto Alegre Sírio e Libanês Hospital Faculty of Pharmacy Medicine Department Federal University of Rio de Janeiro Professor Paulo de Góes Institute of Microbiology Federal University of Rio de Janeiro Pontifical Catholic University of Rio Grande do Sul. Porto Alegre

electrochemical treatment is an alternative modality for tumor treatment based on the application of a low intensity direct electric current to the tumor tissue through two or more platinum electrodes placed within the tumor zone or in the surrounding areas. This treatment is noted for its great effectiveness, minimal invasiveness and local effect. Several studies have been conducted worldwide to evaluate the anfitumoral effect of this therapy. In all these studies a variety of biochemical and physiological responses of tumors to the applied treatment have been obtained. by this reason, researchers have suggested various mechanisms to explain how direct electric current destroys tumor ceils. Although, it is generally accepted this treatment induces electrolysis, electroosmosis and electroporation in tumoral tissues. However, action mechanism of this alternative modality on the tumor tissue is not well understood. Although the principle of electrochemical treatment is simple, a standardized method is not yet available. The mechanism by which electrochemical treatment affects tumor growth and survival may represent more complex process. The present work analyzes the latest and most important research done on the electrochemical treatment of tumors. We conclude with our point of view about the destruction mechanism features of this alternative therapy. Also, we suggest some mechanisms and strategies from the thermodynamic point of view for this therapy. In the area of electrochemical treatment of cancer this tool has been exploited very little and much work remains to be done. electrochemical treatment constitutes a good therapeutic option for patients that have failed the conventional oncology methods.

关键词： Direct electric current electrochemical treatment antimmoral treatment tumor tumor electrolysis rumor entropy dissipative structure

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Above-Optimum Temperature-Induced Differential Photosynthetic and Roots Morphometric Responses in Two Contrasting Rice Genotypes

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Journal of Agricultural Science and Technology(b) 2017年第5期7卷 309-319页

作者： Diogo da Silva Moural Italo Lucas de Moraes Rodrigo da Silva Armestol Rafael Silva da Silva Femanda Reolon Junior borela Paulo Ricardo Reis Fagundes Giovani Greigh de brito Sidnei deuner Institute of biology Department of Botany Federal University of Pelotas (UFPel) Capao do Leao 96010-900 RS Brazil eMbRAPA Temperate Climate Brazilian Agricultural Research Corporation Pelotas 96010-971 RS Brazil

Temperature thresholds during the rice (Oryza sativa L.) cycle development have been reported to affect plant metabolism. Therefore, this study aimed to evaluate the effects of the above-optimal temperature on photosynthetic and morphometric parameters in two contrasting irrigated rice genotypes (bRS Querência—-sensitive and Nagina 22 (N22)—-tolerant). Plants of both genotypes were grown in rhizotrons and always maintained under two temperature conditions: Out—-under environmental temperature conditions, and In—-rhizotrons inside a plastic structure, which increased the temperature about 3 °C to 5 °C above the external one. In the genotype N22, there was an increase (twofold higher) in CO2 assimilation in plants cultivated under elevated temperature (In). In bRS Querência, the stomatal conductance (gs) and transpiration rate (e) decreased upon high temperature. The rise in the temperature also negatively impacted in the modulated fluorescence parameters in both genotypes. Increases in root dry weight (RDW) and shoot dry weight (SDW) were also observed in the genotype N22 when compared to bRS Querência upon temperature rise. In general, the N22 genotype showed greater response to the above-optimal temperature due to its intrinsic thermotolerance traits over bRS Querência. Thus, N22 appeared as a potential donor of heat tolerance genes aiming to obtain new cultivar to face current global warming.

关键词：温度调节遗传型合并新栽培变种分光温度上升 Oryza bRS

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